TY  - JOUR
AU  - Vasić, Tanja
AU  - Vojinović, Uroš
AU  - Žujović, Suzana
AU  - Krnjaja, Vesna
AU  - Živković, Sanja
AU  - Marković, Jordan
AU  - Stević, Milan
PY  - 2019
UR  - https://www.tandfonline.com/eprint/AB5HWK3AX3PVSGGPH3NQ/full?target=10.1080%2F03601234.2019.1653735&
UR  - http://r.istocar.bg.ac.rs/handle/123456789/623
AB  - Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azoxystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.
PB  - Taylor & Francis Inc.
T2  - Journal of Environmental Science and Health, Part B
T1  - In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum
DO  - DOI: 10.1080/03601234.2019.1653735
ER  - 

@article{
author = "Vasić, Tanja and Vojinović, Uroš and Žujović, Suzana and Krnjaja, Vesna and Živković, Sanja and Marković, Jordan and Stević, Milan",
year = "2019",
abstract = "Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azoxystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.",
publisher = "Taylor & Francis Inc.",
journal = "Journal of Environmental Science and Health, Part B",
title = "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum",
doi = "DOI: 10.1080/03601234.2019.1653735"
}

Vasić, T., Vojinović, U., Žujović, S., Krnjaja, V., Živković, S., Marković, J.,& Stević, M.. (2019). In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health, Part B
Taylor & Francis Inc...
https://doi.org/DOI: 10.1080/03601234.2019.1653735

Vasić T, Vojinović U, Žujović S, Krnjaja V, Živković S, Marković J, Stević M. In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health, Part B. 2019;.
doi:DOI: 10.1080/03601234.2019.1653735 .

Vasić, Tanja, Vojinović, Uroš, Žujović, Suzana, Krnjaja, Vesna, Živković, Sanja, Marković, Jordan, Stević, Milan, "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum" in Journal of Environmental Science and Health, Part B (2019),
https://doi.org/DOI: 10.1080/03601234.2019.1653735 . .

TY  - CONF
AU  - Vasić, Tanja
AU  - Krnjaja, Vesna
AU  - Marković, Jordan
AU  - Andjelković, Snežana
AU  - Petrović, Mirjana
AU  - Leposavić, Aleksandra
AU  - Terzić, Dragan
PY  - 2019
UR  - http://agrosym.ues.rs.ba/agrosym/agrosym_2019/BOOK_OF_PROCEEDINGS_2019_FINAL.pdf
UR  - http://r.istocar.bg.ac.rs/handle/123456789/630
AB  - Birdsfoot trefoil (Lotus corniculatus L.) is a species adapted to field conditions in the most important livestock production region in the all continents and constitutes a very valuable forage species. It has a high ability to utilize nutrients and has very modest demands on the conditions of its growth. The roots of birdsfoot trefoil are associated with bacteria that fix atmospheric nitrogen and, thusly, its populations increase the availability of nitrogen in the soil. This species often forms dense, fibrous root networks that reduce soil erosion. Isolation of the pathogen was done from the leaves and roots. Black leaf spots were observed on birdsfoot trefoil in field plots in Serbia. These spots were circular to irregular. Single lesions often coalesced to form larger lesions and became dark brown. On the roots systems of a large number of plants birdsfoot trefoil symptoms of light to dark brown necrosis and discoloration of conductive tissues were observed. There has not been a systematic research of birdsfoot trefoil mycoflora in Serbia. This research aims to present the results of preliminary research of mycopopulation of 12 different genotypes of birdsfoot trefoil. Total of 480 plant parts have been examined and 7 genera of fungi were isolated: Alternaria, Fusarium, Phythophthora, Mucor, Sclerotinia, Bipolaris and Rhizoctonia. Considering the importance of birdsfoot trefoil as a fodder crop in Serbia, the aim of this study was to identify phytopathogenic fungi as casual agents of diseases in birdsfoot trefoil for a clearer perception of problems (the extinction of plants, reducing yields, deterioration of the quality of feed and others) arising as a result of the presence of those fungi.
C3  - Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”
T1  - Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.
SP  - 1025
EP  - 1029
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_630
ER  - 

@conference{
author = "Vasić, Tanja and Krnjaja, Vesna and Marković, Jordan and Andjelković, Snežana and Petrović, Mirjana and Leposavić, Aleksandra and Terzić, Dragan",
year = "2019",
abstract = "Birdsfoot trefoil (Lotus corniculatus L.) is a species adapted to field conditions in the most important livestock production region in the all continents and constitutes a very valuable forage species. It has a high ability to utilize nutrients and has very modest demands on the conditions of its growth. The roots of birdsfoot trefoil are associated with bacteria that fix atmospheric nitrogen and, thusly, its populations increase the availability of nitrogen in the soil. This species often forms dense, fibrous root networks that reduce soil erosion. Isolation of the pathogen was done from the leaves and roots. Black leaf spots were observed on birdsfoot trefoil in field plots in Serbia. These spots were circular to irregular. Single lesions often coalesced to form larger lesions and became dark brown. On the roots systems of a large number of plants birdsfoot trefoil symptoms of light to dark brown necrosis and discoloration of conductive tissues were observed. There has not been a systematic research of birdsfoot trefoil mycoflora in Serbia. This research aims to present the results of preliminary research of mycopopulation of 12 different genotypes of birdsfoot trefoil. Total of 480 plant parts have been examined and 7 genera of fungi were isolated: Alternaria, Fusarium, Phythophthora, Mucor, Sclerotinia, Bipolaris and Rhizoctonia. Considering the importance of birdsfoot trefoil as a fodder crop in Serbia, the aim of this study was to identify phytopathogenic fungi as casual agents of diseases in birdsfoot trefoil for a clearer perception of problems (the extinction of plants, reducing yields, deterioration of the quality of feed and others) arising as a result of the presence of those fungi.",
journal = "Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”",
title = "Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.",
pages = "1025-1029",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_630"
}

Vasić, T., Krnjaja, V., Marković, J., Andjelković, S., Petrović, M., Leposavić, A.,& Terzić, D.. (2019). Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.. in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”, 1025-1029.
https://hdl.handle.net/21.15107/rcub_ristocar_630

Vasić T, Krnjaja V, Marković J, Andjelković S, Petrović M, Leposavić A, Terzić D. Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.. in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”. 2019;:1025-1029.
https://hdl.handle.net/21.15107/rcub_ristocar_630 .

Vasić, Tanja, Krnjaja, Vesna, Marković, Jordan, Andjelković, Snežana, Petrović, Mirjana, Leposavić, Aleksandra, Terzić, Dragan, "Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia." in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019” (2019):1025-1029,
https://hdl.handle.net/21.15107/rcub_ristocar_630 .

TY  - JOUR
AU  - Brankov, M.
AU  - Dragičević, Vesna
AU  - Simic, M.
AU  - Filipović, M.
AU  - Kresović, M.
AU  - Mandić, Violeta
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/535
AB  - Dominance of grass weeds in maize crop occurs due to a lack of selective herbicides for their control. With sulphonylurea herbicides this problem became under control, but a problem with selectivity was developed, particularly in maize seed crop. The effect of sulphonylureas and foliar fertiliser on maize lines was evaluated by visual estimation, grain yield, as well as the alterations in the content of antioxidants: free thiolic groups, phenolics and soluble proteins in the leaves. The proteins content did not vary significantly under the influence of herbicides, compared to the control, opposite to free thiolic groups and phenolics. The differences in the content of phenolics and thiolic groups in the treatments with herbicides plus foliar fertiliser indicated that herbicide stress was more rapidly overcome. Most of the genotypes expressed significant increase of grain yield in the treatments with foliar fertiliser, compared to control and analogous treatments with herbicides.
PB  - Scibulcom Ltd, Sofia
T2  - Journal of Environmental Protection and Ecology
T1  - Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser
VL  - 18
IS  - 4
SP  - 1440
EP  - 1449
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_535
ER  - 

@article{
author = "Brankov, M. and Dragičević, Vesna and Simic, M. and Filipović, M. and Kresović, M. and Mandić, Violeta",
year = "2017",
abstract = "Dominance of grass weeds in maize crop occurs due to a lack of selective herbicides for their control. With sulphonylurea herbicides this problem became under control, but a problem with selectivity was developed, particularly in maize seed crop. The effect of sulphonylureas and foliar fertiliser on maize lines was evaluated by visual estimation, grain yield, as well as the alterations in the content of antioxidants: free thiolic groups, phenolics and soluble proteins in the leaves. The proteins content did not vary significantly under the influence of herbicides, compared to the control, opposite to free thiolic groups and phenolics. The differences in the content of phenolics and thiolic groups in the treatments with herbicides plus foliar fertiliser indicated that herbicide stress was more rapidly overcome. Most of the genotypes expressed significant increase of grain yield in the treatments with foliar fertiliser, compared to control and analogous treatments with herbicides.",
publisher = "Scibulcom Ltd, Sofia",
journal = "Journal of Environmental Protection and Ecology",
title = "Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser",
volume = "18",
number = "4",
pages = "1440-1449",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_535"
}

Brankov, M., Dragičević, V., Simic, M., Filipović, M., Kresović, M.,& Mandić, V.. (2017). Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser. in Journal of Environmental Protection and Ecology
Scibulcom Ltd, Sofia., 18(4), 1440-1449.
https://hdl.handle.net/21.15107/rcub_ristocar_535

Brankov M, Dragičević V, Simic M, Filipović M, Kresović M, Mandić V. Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser. in Journal of Environmental Protection and Ecology. 2017;18(4):1440-1449.
https://hdl.handle.net/21.15107/rcub_ristocar_535 .

Brankov, M., Dragičević, Vesna, Simic, M., Filipović, M., Kresović, M., Mandić, Violeta, "Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser" in Journal of Environmental Protection and Ecology, 18, no. 4 (2017):1440-1449,
https://hdl.handle.net/21.15107/rcub_ristocar_535 .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Stanković, Slavica Ž.
AU  - Terzic, Dragan
AU  - Milenković, Jasmina
AU  - Marković, Jordan
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/484
AB  - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB  - Serbian Genetics Society, Belgrade
T2  - Genetika-Belgrade
T1  - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL  - 48
IS  - 1
SP  - 187
EP  - 198
DO  - 10.2298/GENSR1601187V
ER  - 

@article{
author = "Vasić, Tanja and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica Ž. and Terzic, Dragan and Milenković, Jasmina and Marković, Jordan",
year = "2016",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Serbian Genetics Society, Belgrade",
journal = "Genetika-Belgrade",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V"
}

Vasić, T., Krnjaja, V., Jevremović, D., Stanković, S. Ž., Terzic, D., Milenković, J.,& Marković, J.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade
Serbian Genetics Society, Belgrade., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V

Vasić T, Krnjaja V, Jevremović D, Stanković SŽ, Terzic D, Milenković J, Marković J. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade. 2016;48(1):187-198.
doi:10.2298/GENSR1601187V .

Vasić, Tanja, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica Ž., Terzic, Dragan, Milenković, Jasmina, Marković, Jordan, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika-Belgrade, 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Vasić, Tanja
PY  - 2013
UR  - http://r.istocar.bg.ac.rs/handle/123456789/374
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Vasić, Tanja",
year = "2013",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž.,& Vasić, T.. (2013). The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K

Krnjaja V, Lević J, Stanković SŽ, Vasić T. The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina. 2013;28(3):157-165.
doi:10.2298/PIF1303157K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Vasić, Tanja, "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi" in Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K . .