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In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum

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2019
Authors
Vasić, Tanja
Vojinović, Uroš
Žujović, Suzana
Krnjaja, Vesna
Živković, Sanja
Marković, Jordan
Stević, Milan
Article (Published version)
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Abstract
Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azo...xystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.

Keywords:
Fungicide sensitivity / DMI / Qol / SDHI / molecular characterization / pathogenicity
Source:
Journal of Environmental Science and Health, Part B, 2019
Publisher:
  • Taylor & Francis Inc.
Projects:
  • Improvement of genetic potential and technologies in forage crops production in function of sustainable animal husbandry development (RS-31057)

DOI: DOI: 10.1080/03601234.2019.1653735

ISSN: 0360-1234

[ Google Scholar ]
URI
https://www.tandfonline.com/eprint/AB5HWK3AX3PVSGGPH3NQ/full?target=10.1080%2F03601234.2019.1653735&
http://r.istocar.bg.ac.rs/handle/123456789/623
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