TY  - JOUR
AU  - Zorc, Minja
AU  - Škorput, Dubravko
AU  - Gvozdanović, Kristina
AU  - Margeta, Polona
AU  - Karolyi, Danijel
AU  - Luković, Zoran
AU  - Salajpal, Krešimir
AU  - Savić, Radomir
AU  - Munoz, Maria
AU  - Bovo, Samuele
AU  - Đurkin Kušec, Ivona
AU  - Radović, Čedomir
AU  - Kušec, Goran
AU  - Čandek Potokar, Marjeta
AU  - Dovč, Peter
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/818
AB  - Background: The importance of local breeds as genetic reservoirs of valuable genetic variation is well established.
Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local
pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from
Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka
and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide
polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were
compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and
identifed genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS).
Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka
and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed.
The analysis of admixture between breeds revealed potential gene fow between the Mangalitsa and Moravka, and
between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and
Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identifed
genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which
harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS
approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance.
Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the
existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to
estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed
genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under
selection pressure.
PB  - BMC
T2  - Genetic Selection Evolution
T1  - Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia
DO  - https://doi.org/10.1186/s12711-022-00718-6
ER  - 

@article{
author = "Zorc, Minja and Škorput, Dubravko and Gvozdanović, Kristina and Margeta, Polona and Karolyi, Danijel and Luković, Zoran and Salajpal, Krešimir and Savić, Radomir and Munoz, Maria and Bovo, Samuele and Đurkin Kušec, Ivona and Radović, Čedomir and Kušec, Goran and Čandek Potokar, Marjeta and Dovč, Peter",
year = "2022",
abstract = "Background: The importance of local breeds as genetic reservoirs of valuable genetic variation is well established.
Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local
pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from
Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka
and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide
polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were
compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and
identifed genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS).
Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka
and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed.
The analysis of admixture between breeds revealed potential gene fow between the Mangalitsa and Moravka, and
between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and
Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identifed
genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which
harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS
approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance.
Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the
existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to
estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed
genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under
selection pressure.",
publisher = "BMC",
journal = "Genetic Selection Evolution",
title = "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia",
doi = "https://doi.org/10.1186/s12711-022-00718-6"
}

Zorc, M., Škorput, D., Gvozdanović, K., Margeta, P., Karolyi, D., Luković, Z., Salajpal, K., Savić, R., Munoz, M., Bovo, S., Đurkin Kušec, I., Radović, Č., Kušec, G., Čandek Potokar, M.,& Dovč, P.. (2022). Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetic Selection Evolution
BMC..
https://doi.org/https://doi.org/10.1186/s12711-022-00718-6

Zorc M, Škorput D, Gvozdanović K, Margeta P, Karolyi D, Luković Z, Salajpal K, Savić R, Munoz M, Bovo S, Đurkin Kušec I, Radović Č, Kušec G, Čandek Potokar M, Dovč P. Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetic Selection Evolution. 2022;.
doi:https://doi.org/10.1186/s12711-022-00718-6 .

Zorc, Minja, Škorput, Dubravko, Gvozdanović, Kristina, Margeta, Polona, Karolyi, Danijel, Luković, Zoran, Salajpal, Krešimir, Savić, Radomir, Munoz, Maria, Bovo, Samuele, Đurkin Kušec, Ivona, Radović, Čedomir, Kušec, Goran, Čandek Potokar, Marjeta, Dovč, Peter, "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia" in Genetic Selection Evolution (2022),
https://doi.org/https://doi.org/10.1186/s12711-022-00718-6 . .

TY  - JOUR
AU  - Bozzi, Riccardo
AU  - Skrlep, M.
AU  - Lenoir, Herveline
AU  - Lebret, Benedicte
AU  - Garcia Gasco, J. M.
AU  - Petig, M.
AU  - Charneca, Rui
AU  - Paixim, H.
AU  - Karolyi, Danijel
AU  - Radović, Čedomir
AU  - Gallo, Maurizio
AU  - Geraci, Claudia
AU  - Usai, G.
AU  - Quintanilla, R.
AU  - Araujo, José Pedro
AU  - Razmaite, V.
AU  - Gvozdanović, Kristina
PY  - 2018
UR  - https://www.uco.es/ucopress/az/index.php/az/article/view/3560
UR  - http://epserbia.open.ac.rs/123456789/191
UR  - http://r.istocar.bg.ac.rs/handle/123456789/577
UR  - https://prodinra.inra.fr/record/430277
AB  - The paper reports the results of a survey on the demographic and phenotypic characterization of 20 European local pig breeds involved in the H2020 TREASURE project including information on: demographic parameters, main morphological features, reproductive information, additional information collected at herd-level (i.e. temperament, holding, mating practices), origins and development of the breeds. Almost all the breeds (18 out of 20) possess a herd book even if the starting year is highly variable (from 1980 to 2006). Number of breeding females ranged from 24 (Moravka) to over 200,000 heads (Ibérico). Male/female ratio varied greatly with the highest values for the Italian breeds probably due to the different policy of animal recording. Almost all the breeds undergo a conservation program whereas really few are interested by other conservation techniques and for less than five breeds data related to effective number and inbreeding coefficient are easily available. Average values for teat number, litter size and weaned piglets are 12, 8 and 6 respectively with a great potential for their improvement. The depicted scenario is highly diversified and the data collected represent the starting point for the achievement of a collective trademark under the umbrella of the TREASURE project.
PB  - Cordoba : UCOPress. Cordoba University Press
T2  - Archivos de Zootecnia
T1  - Survey of demographic and phenotypic data of local pig breeds of TREASURE project
VL  - 67
IS  - Supplement
SP  - 1
EP  - 4
DO  - 10.21071/az.v67iSupplement.3560
ER  - 

@article{
author = "Bozzi, Riccardo and Skrlep, M. and Lenoir, Herveline and Lebret, Benedicte and Garcia Gasco, J. M. and Petig, M. and Charneca, Rui and Paixim, H. and Karolyi, Danijel and Radović, Čedomir and Gallo, Maurizio and Geraci, Claudia and Usai, G. and Quintanilla, R. and Araujo, José Pedro and Razmaite, V. and Gvozdanović, Kristina",
year = "2018",
abstract = "The paper reports the results of a survey on the demographic and phenotypic characterization of 20 European local pig breeds involved in the H2020 TREASURE project including information on: demographic parameters, main morphological features, reproductive information, additional information collected at herd-level (i.e. temperament, holding, mating practices), origins and development of the breeds. Almost all the breeds (18 out of 20) possess a herd book even if the starting year is highly variable (from 1980 to 2006). Number of breeding females ranged from 24 (Moravka) to over 200,000 heads (Ibérico). Male/female ratio varied greatly with the highest values for the Italian breeds probably due to the different policy of animal recording. Almost all the breeds undergo a conservation program whereas really few are interested by other conservation techniques and for less than five breeds data related to effective number and inbreeding coefficient are easily available. Average values for teat number, litter size and weaned piglets are 12, 8 and 6 respectively with a great potential for their improvement. The depicted scenario is highly diversified and the data collected represent the starting point for the achievement of a collective trademark under the umbrella of the TREASURE project.",
publisher = "Cordoba : UCOPress. Cordoba University Press",
journal = "Archivos de Zootecnia",
title = "Survey of demographic and phenotypic data of local pig breeds of TREASURE project",
volume = "67",
number = "Supplement",
pages = "1-4",
doi = "10.21071/az.v67iSupplement.3560"
}

Bozzi, R., Skrlep, M., Lenoir, H., Lebret, B., Garcia Gasco, J. M., Petig, M., Charneca, R., Paixim, H., Karolyi, D., Radović, Č., Gallo, M., Geraci, C., Usai, G., Quintanilla, R., Araujo, J. P., Razmaite, V.,& Gvozdanović, K.. (2018). Survey of demographic and phenotypic data of local pig breeds of TREASURE project. in Archivos de Zootecnia
Cordoba : UCOPress. Cordoba University Press., 67(Supplement), 1-4.
https://doi.org/10.21071/az.v67iSupplement.3560

Bozzi R, Skrlep M, Lenoir H, Lebret B, Garcia Gasco JM, Petig M, Charneca R, Paixim H, Karolyi D, Radović Č, Gallo M, Geraci C, Usai G, Quintanilla R, Araujo JP, Razmaite V, Gvozdanović K. Survey of demographic and phenotypic data of local pig breeds of TREASURE project. in Archivos de Zootecnia. 2018;67(Supplement):1-4.
doi:10.21071/az.v67iSupplement.3560 .

Bozzi, Riccardo, Skrlep, M., Lenoir, Herveline, Lebret, Benedicte, Garcia Gasco, J. M., Petig, M., Charneca, Rui, Paixim, H., Karolyi, Danijel, Radović, Čedomir, Gallo, Maurizio, Geraci, Claudia, Usai, G., Quintanilla, R., Araujo, José Pedro, Razmaite, V., Gvozdanović, Kristina, "Survey of demographic and phenotypic data of local pig breeds of TREASURE project" in Archivos de Zootecnia, 67, no. Supplement (2018):1-4,
https://doi.org/10.21071/az.v67iSupplement.3560 . .

TY  - CONF
AU  - Bozzi, Riccardo
AU  - Škrlep, Martin
AU  - Lenoir, Herveline
AU  - Lebret, Benedicte
AU  - Garcia Casco, Juan Maria
AU  - Petig, Matthias
AU  - Charneca, Rui
AU  - Paixim, Hugo
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Geraci, Claudia
AU  - Usai, Graziano
AU  - Quintanilla, Raquel
AU  - Araujo, José Pedro
AU  - Razmaite, Violeta
AU  - Gvozdanović, Kristina
AU  - Radović, Čedomir
PY  - 2017
UR  - https://zenodo.org/record/344953
UR  - http://epserbia.open.ac.rs/123456789/188
UR  - http://r.istocar.bg.ac.rs/handle/123456789/569
UR  - http://hdl.handle.net/2158/1071637
UR  - https://hal.archives-ouvertes.fr/hal-01603537
AB  - Local genetic resources represent an important reservoir of biodiversity in the animal sector. Sustainable use of these resources can be an important source of income for farmers taking advantage of the peculiar characteristics of their products.
PB  - Portalegre : Instituto Politécnico de Portalegre
C3  - Book of Abstracts of the 9th International Symposium on Mediterranean Pig, 3-5, November, 2016, Portalegre, Portugal
T1  - Survey of Demographic and Phenotypic Data of Local Pig Breeds of Treasure Project
DO  - 10.5281/zenodo.344953
ER  - 

@conference{
author = "Bozzi, Riccardo and Škrlep, Martin and Lenoir, Herveline and Lebret, Benedicte and Garcia Casco, Juan Maria and Petig, Matthias and Charneca, Rui and Paixim, Hugo and Karolyi, Danijel and Gallo, Maurizio and Geraci, Claudia and Usai, Graziano and Quintanilla, Raquel and Araujo, José Pedro and Razmaite, Violeta and Gvozdanović, Kristina and Radović, Čedomir",
year = "2017",
abstract = "Local genetic resources represent an important reservoir of biodiversity in the animal sector. Sustainable use of these resources can be an important source of income for farmers taking advantage of the peculiar characteristics of their products.",
publisher = "Portalegre : Instituto Politécnico de Portalegre",
journal = "Book of Abstracts of the 9th International Symposium on Mediterranean Pig, 3-5, November, 2016, Portalegre, Portugal",
title = "Survey of Demographic and Phenotypic Data of Local Pig Breeds of Treasure Project",
doi = "10.5281/zenodo.344953"
}

Bozzi, R., Škrlep, M., Lenoir, H., Lebret, B., Garcia Casco, J. M., Petig, M., Charneca, R., Paixim, H., Karolyi, D., Gallo, M., Geraci, C., Usai, G., Quintanilla, R., Araujo, J. P., Razmaite, V., Gvozdanović, K.,& Radović, Č.. (2017). Survey of Demographic and Phenotypic Data of Local Pig Breeds of Treasure Project. in Book of Abstracts of the 9th International Symposium on Mediterranean Pig, 3-5, November, 2016, Portalegre, Portugal
Portalegre : Instituto Politécnico de Portalegre..
https://doi.org/10.5281/zenodo.344953

Bozzi R, Škrlep M, Lenoir H, Lebret B, Garcia Casco JM, Petig M, Charneca R, Paixim H, Karolyi D, Gallo M, Geraci C, Usai G, Quintanilla R, Araujo JP, Razmaite V, Gvozdanović K, Radović Č. Survey of Demographic and Phenotypic Data of Local Pig Breeds of Treasure Project. in Book of Abstracts of the 9th International Symposium on Mediterranean Pig, 3-5, November, 2016, Portalegre, Portugal. 2017;.
doi:10.5281/zenodo.344953 .

Bozzi, Riccardo, Škrlep, Martin, Lenoir, Herveline, Lebret, Benedicte, Garcia Casco, Juan Maria, Petig, Matthias, Charneca, Rui, Paixim, Hugo, Karolyi, Danijel, Gallo, Maurizio, Geraci, Claudia, Usai, Graziano, Quintanilla, Raquel, Araujo, José Pedro, Razmaite, Violeta, Gvozdanović, Kristina, Radović, Čedomir, "Survey of Demographic and Phenotypic Data of Local Pig Breeds of Treasure Project" in Book of Abstracts of the 9th International Symposium on Mediterranean Pig, 3-5, November, 2016, Portalegre, Portugal (2017),
https://doi.org/10.5281/zenodo.344953 . .