TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/970
AB  - The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022
VL  - 39
IS  - 2
SP  - 205
EP  - 218
DO  - https://doi.org/10.2298/BAH2302205K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022",
volume = "39",
number = "2",
pages = "205-218",
doi = "https://doi.org/10.2298/BAH2302205K"
}

Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 39(2), 205-218.
https://doi.org/https://doi.org/10.2298/BAH2302205K

Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry. 2023;39(2):205-218.
doi:https://doi.org/10.2298/BAH2302205K .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022" in Biotechnology in Animal Husbandry, 39, no. 2 (2023):205-218,
https://doi.org/https://doi.org/10.2298/BAH2302205K . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/905
AB  - Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_905
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_905"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):80-80,
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/904
AB  - Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_904
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_904"
}

Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904

Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):79-79,
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/903
AB  - Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 566
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_903
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "566-576",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_903"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. 2023;:566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia (2023):566-576,
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Radović, Čedomir
PY  - 2021-02-25
UR  - http://r.istocar.bg.ac.rs/handle/123456789/730
AB  - The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.
T2  - Crop Protection
T1  - Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels
VL  - 144
SP  - 105601
DO  - https://doi.org/10.1016/j.cropro.2021.105601
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Radović, Čedomir",
year = "2021-02-25",
abstract = "The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.",
journal = "Crop Protection",
title = "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels",
volume = "144",
pages = "105601",
doi = "https://doi.org/10.1016/j.cropro.2021.105601"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Radović, Č.. (2021-02-25). Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection, 144, 105601.
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Vasić T, Radović Č. Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection. 2021;144:105601.
doi:https://doi.org/10.1016/j.cropro.2021.105601 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Radović, Čedomir, "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels" in Crop Protection, 144 (2021-02-25):105601,
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Petrović, Tanja
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Radović, Čedomir
AU  - Delić, Nikola
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/732
AB  - The aim of this study was to establish the total fungal (mould)
count in 79 commercial pig farm feed samples (42 samples for piglets, 29 samples 
for fattening pigs and 8 samples for sows) collected from the Serbian feed 
producers during the three-year period (2017–2019), as well as to evaluate the
percentage of contamination samples by fungi, especially species from Aspergillus,
Fusarium and Penicillium genera. Using standard mycological methods, the total 
count and identification of fungi were determined. Total fungal count was ranging 
from 1 × 102 to 1.41 × 105 cfu g–1 in the feed samples for piglets. Furthermore, in 
the feed samplesfor fattening pigs it ranged from 1 × 102 to 2.54 × 105 cfu g–1
, and 
from 1 × 102 to 1.93 × 105 cfu g–1 in the feed samples for sows. It has established 
the impermissible limit of total fungal count in 3.45% and 4.76% of a feed for 
fattening pigs and feed for piglets, respectively. Statistical analysis of the total 
number of fungi did not establish significant differences between the examined 
feed groups. Fusarium species were present in the most feed samples for fattening 
pigs (65.52%), followed by the feed for sows (62.50%) and piglets (47.62%). 
Aspergillus species were determined in 59.52, 58.62 and 37.50% feed samples for 
piglets, fattening pigs and sows, respectively. Penicillium species contaminated the 
lowest percentage of feed samples for fattening pigs (27.59%) and the highest 
percentage of feed samples for sows (37.50%). In a small number of samples 
Alternaria, Mucor, and Rhizopus species were identified. Based on these results, it 
can be concluded that the investigation of fungal contamination is an important 
indicator of a hygienic condition of feed intended for the nutrition of farm pigs. In 
addition, the percentage of fungal contamination of the examined samples indicates 
a potentially high risk to animal health. Due to that, a regular mycological 
evaluation is necessary to assess nutritional quality as one of the fundamental 
criteria for feed safety.
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of pig farm feeds
VL  - 37
IS  - 2
SP  - 139
EP  - 147
DO  - 10.2298/BAH2102139K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Radović, Čedomir and Delić, Nikola",
year = "2021",
abstract = "The aim of this study was to establish the total fungal (mould)
count in 79 commercial pig farm feed samples (42 samples for piglets, 29 samples 
for fattening pigs and 8 samples for sows) collected from the Serbian feed 
producers during the three-year period (2017–2019), as well as to evaluate the
percentage of contamination samples by fungi, especially species from Aspergillus,
Fusarium and Penicillium genera. Using standard mycological methods, the total 
count and identification of fungi were determined. Total fungal count was ranging 
from 1 × 102 to 1.41 × 105 cfu g–1 in the feed samples for piglets. Furthermore, in 
the feed samplesfor fattening pigs it ranged from 1 × 102 to 2.54 × 105 cfu g–1
, and 
from 1 × 102 to 1.93 × 105 cfu g–1 in the feed samples for sows. It has established 
the impermissible limit of total fungal count in 3.45% and 4.76% of a feed for 
fattening pigs and feed for piglets, respectively. Statistical analysis of the total 
number of fungi did not establish significant differences between the examined 
feed groups. Fusarium species were present in the most feed samples for fattening 
pigs (65.52%), followed by the feed for sows (62.50%) and piglets (47.62%). 
Aspergillus species were determined in 59.52, 58.62 and 37.50% feed samples for 
piglets, fattening pigs and sows, respectively. Penicillium species contaminated the 
lowest percentage of feed samples for fattening pigs (27.59%) and the highest 
percentage of feed samples for sows (37.50%). In a small number of samples 
Alternaria, Mucor, and Rhizopus species were identified. Based on these results, it 
can be concluded that the investigation of fungal contamination is an important 
indicator of a hygienic condition of feed intended for the nutrition of farm pigs. In 
addition, the percentage of fungal contamination of the examined samples indicates 
a potentially high risk to animal health. Due to that, a regular mycological 
evaluation is necessary to assess nutritional quality as one of the fundamental 
criteria for feed safety.",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of pig farm feeds",
volume = "37",
number = "2",
pages = "139-147",
doi = "10.2298/BAH2102139K"
}

Krnjaja, V., Stanojković, A., Petrović, T., Mandić, V., Bijelić, Z., Radović, Č.,& Delić, N.. (2021). Fungal contamination of pig farm feeds. in Biotechnology in Animal Husbandry, 37(2), 139-147.
https://doi.org/10.2298/BAH2102139K

Krnjaja V, Stanojković A, Petrović T, Mandić V, Bijelić Z, Radović Č, Delić N. Fungal contamination of pig farm feeds. in Biotechnology in Animal Husbandry. 2021;37(2):139-147.
doi:10.2298/BAH2102139K .

Krnjaja, Vesna, Stanojković, Aleksandar, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Radović, Čedomir, Delić, Nikola, "Fungal contamination of pig farm feeds" in Biotechnology in Animal Husbandry, 37, no. 2 (2021):139-147,
https://doi.org/10.2298/BAH2102139K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Lukić, Miloš
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Nikolić, Milica
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/682
AB  - The objective of the present study was to investigate the susceptibility of maize hybrids to the natural occurrence of toxigenic fungal species, in particular toxigenic Aspergillus and Fusarium species, and mycotoxins (aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins B1, B2 and B3 (FBs)). Grain samples of six commercial maize hybrids (MAS 34.B – FAO 300, MAS 40.F, MAS 48.L, KWS Konfites and ZP 427 – FAO 400, and MAS 56.A – FAO 500) were collected at harvest in 2018. A total of seven fungal genera, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium and Rhizopus, were identified of which only species from the genus Fusarium were present on maize grains of all hybrids tested. The incidence of Fusarium spp. was higher in the hybrids MAS 48.L (54.6%), and MAS 56.A (53.3%), compared to MAS 40.F (37.3%), KWS Konfites (28%), MAS 34.B (22.6%) and ZP 427 (12%) hybrids. Among the identified Fusarium species (F. graminearum, F. proliferatum, F. subglutinans and F. verticillioides), F. proliferatum was present in all hybrids, ranging from 9.3% (ZP 427) to 30.7% (MAS 48.L ), whereas F. subglutinans was present in two hybrids, MAS 40.F (16%) and MAS 56.A (9.3%). The incidence of F. graminearum ranged from 0% (KWS Konfites) to 9.3% (MAS 34.B), while the incidence of F. verticillioides ranged from 0% (MAS 34.B and ZP 427) to 21.3% (MAS 48.L). In the samples, Aspergillus species were not identified. The effect of maize hybrids was significant on the level of mycotoxins. MAS 34.B hybrid had a statistically significantly higher levels of AFB1 and DON than other hybrids. The FBs level was the highest in the hybrid MAS 34.B (1202 μg kg-1) and the lowest in the hybrid KWS Konfites (88.33 μg kg-1). However, the FBs level did not differ between hybrids MAS 34.B, MAS 40.F, and MAS 56.L, MAS 40.F, MAS 48.L, and MAS 56.A, and KWS Konfites and ZP 427. In all hybrids, AFB1, DON, and FBs levels were below the maximum permissible levels stipulated by the legislation of the European Union and the Republic of Serbia in unprocessed maize.
The agro-ecological conditions in 2018 favored the development of Fusarium species on maize grains of the hybrids tested, especially fumonisin producing species.
T2  - Biotechnology in Animal Husbandry
T1  - Natural toxigenic fungal and mycotoxin occurrence in maize hybrids
VL  - 36
IS  - 1
SP  - 75
EP  - 85
DO  - 10.2298/BAH2001075K
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Lukić, Miloš and Petrović, Tanja and Stanković, Slavica and Nikolić, Milica",
year = "2020",
abstract = "The objective of the present study was to investigate the susceptibility of maize hybrids to the natural occurrence of toxigenic fungal species, in particular toxigenic Aspergillus and Fusarium species, and mycotoxins (aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins B1, B2 and B3 (FBs)). Grain samples of six commercial maize hybrids (MAS 34.B – FAO 300, MAS 40.F, MAS 48.L, KWS Konfites and ZP 427 – FAO 400, and MAS 56.A – FAO 500) were collected at harvest in 2018. A total of seven fungal genera, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium and Rhizopus, were identified of which only species from the genus Fusarium were present on maize grains of all hybrids tested. The incidence of Fusarium spp. was higher in the hybrids MAS 48.L (54.6%), and MAS 56.A (53.3%), compared to MAS 40.F (37.3%), KWS Konfites (28%), MAS 34.B (22.6%) and ZP 427 (12%) hybrids. Among the identified Fusarium species (F. graminearum, F. proliferatum, F. subglutinans and F. verticillioides), F. proliferatum was present in all hybrids, ranging from 9.3% (ZP 427) to 30.7% (MAS 48.L ), whereas F. subglutinans was present in two hybrids, MAS 40.F (16%) and MAS 56.A (9.3%). The incidence of F. graminearum ranged from 0% (KWS Konfites) to 9.3% (MAS 34.B), while the incidence of F. verticillioides ranged from 0% (MAS 34.B and ZP 427) to 21.3% (MAS 48.L). In the samples, Aspergillus species were not identified. The effect of maize hybrids was significant on the level of mycotoxins. MAS 34.B hybrid had a statistically significantly higher levels of AFB1 and DON than other hybrids. The FBs level was the highest in the hybrid MAS 34.B (1202 μg kg-1) and the lowest in the hybrid KWS Konfites (88.33 μg kg-1). However, the FBs level did not differ between hybrids MAS 34.B, MAS 40.F, and MAS 56.L, MAS 40.F, MAS 48.L, and MAS 56.A, and KWS Konfites and ZP 427. In all hybrids, AFB1, DON, and FBs levels were below the maximum permissible levels stipulated by the legislation of the European Union and the Republic of Serbia in unprocessed maize.
The agro-ecological conditions in 2018 favored the development of Fusarium species on maize grains of the hybrids tested, especially fumonisin producing species.",
journal = "Biotechnology in Animal Husbandry",
title = "Natural toxigenic fungal and mycotoxin occurrence in maize hybrids",
volume = "36",
number = "1",
pages = "75-85",
doi = "10.2298/BAH2001075K"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Lukić, M., Petrović, T., Stanković, S.,& Nikolić, M.. (2020). Natural toxigenic fungal and mycotoxin occurrence in maize hybrids. in Biotechnology in Animal Husbandry, 36(1), 75-85.
https://doi.org/10.2298/BAH2001075K

Krnjaja V, Mandić V, Bijelić Z, Lukić M, Petrović T, Stanković S, Nikolić M. Natural toxigenic fungal and mycotoxin occurrence in maize hybrids. in Biotechnology in Animal Husbandry. 2020;36(1):75-85.
doi:10.2298/BAH2001075K .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Lukić, Miloš, Petrović, Tanja, Stanković, Slavica, Nikolić, Milica, "Natural toxigenic fungal and mycotoxin occurrence in maize hybrids" in Biotechnology in Animal Husbandry, 36, no. 1 (2020):75-85,
https://doi.org/10.2298/BAH2001075K . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Miličić, Danijel
AU  - Petrović, Tanja
AU  - Krnjaja, Vesna
AU  - Stanojević, Slađana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/609
AB  - Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a w values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.
PB  - MDPI, Basel, Switzerland
T2  - Toxins
T1  - Mycotoxins and mycotoxin producing fungi in pollen: Review
VL  - 11
IS  - 2
SP  - 64
DO  - 10.3390/toxins11020064
ER  - 

@article{
author = "Kostić, Aleksandar and Miličić, Danijel and Petrović, Tanja and Krnjaja, Vesna and Stanojević, Slađana and Barać, Miroljub and Tešić, Živoslav and Pešić, Mirjana",
year = "2019",
abstract = "Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a w values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.",
publisher = "MDPI, Basel, Switzerland",
journal = "Toxins",
title = "Mycotoxins and mycotoxin producing fungi in pollen: Review",
volume = "11",
number = "2",
pages = "64",
doi = "10.3390/toxins11020064"
}

Kostić, A., Miličić, D., Petrović, T., Krnjaja, V., Stanojević, S., Barać, M., Tešić, Ž.,& Pešić, M.. (2019). Mycotoxins and mycotoxin producing fungi in pollen: Review. in Toxins
MDPI, Basel, Switzerland., 11(2), 64.
https://doi.org/10.3390/toxins11020064

Kostić A, Miličić D, Petrović T, Krnjaja V, Stanojević S, Barać M, Tešić Ž, Pešić M. Mycotoxins and mycotoxin producing fungi in pollen: Review. in Toxins. 2019;11(2):64.
doi:10.3390/toxins11020064 .

Kostić, Aleksandar, Miličić, Danijel, Petrović, Tanja, Krnjaja, Vesna, Stanojević, Slađana, Barać, Miroljub, Tešić, Živoslav, Pešić, Mirjana, "Mycotoxins and mycotoxin producing fungi in pollen: Review" in Toxins, 11, no. 2 (2019):64,
https://doi.org/10.3390/toxins11020064 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://istocar.bg.ac.rs/wp-content/uploads/2019/03/06.BAH-19-1-10-26.2.2019.-Krnjaja-et-al-1-1.pdf
UR  - http://r.istocar.bg.ac.rs/handle/123456789/610
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method.
The total fungal count was in the range from 1 x 102 (2 log CFU g–1) to 1.83 x 105 CFU g–1 (5.26 log CFU g–1). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g–1, whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g–1. In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 μg kg–1, with an average of 4.47 and 4.56 μg kg–1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits.
The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz razliĉitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razređenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA).
Ukupan broj gljiva bio je od 1 x 102 (2 log CFU g–1) do 1,83 x 105 CFU g–1 (5.26 log CFU g–1). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g–1, dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g–1. U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 μg kg–1, sa proseĉnim sadržajem od 4,47 μg kg–1 u uzorcima hrane za piliće, i 4,56 μg kg–1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju znaĉaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljiviĉnih) kontaminenata u lancu ishrane.
PB  - Belgrade : Institute for animal husbandry
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 

@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method.
The total fungal count was in the range from 1 x 102 (2 log CFU g–1) to 1.83 x 105 CFU g–1 (5.26 log CFU g–1). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g–1, whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g–1. In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 μg kg–1, with an average of 4.47 and 4.56 μg kg–1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits.
The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz razliĉitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razređenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA).
Ukupan broj gljiva bio je od 1 x 102 (2 log CFU g–1) do 1,83 x 105 CFU g–1 (5.26 log CFU g–1). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g–1, dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g–1. U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 μg kg–1, sa proseĉnim sadržajem od 4,47 μg kg–1 u uzorcima hrane za piliće, i 4,56 μg kg–1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju znaĉaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljiviĉnih) kontaminenata u lancu ishrane.",
publisher = "Belgrade : Institute for animal husbandry",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}

Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z.. (2019). Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry
Belgrade : Institute for animal husbandry., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K

Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry. 2019;35(1):61-69.
doi:10.2298/BAH1901061K .

Krnjaja, Vesna, Petrović, Tanja, Stanković, Slavica, Lukić, Miloš, Škrbić, Zdenka, Mandić, Violeta, Bijelić, Zorica, "Mycobiota and aflatoxin B1 in poultry feeds" in Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Jauković, Marko
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/626
AB  - Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
T1  - The effect of climate conditions on aflatoxin contamination of cereal grains and feeds
SP  - 580
EP  - 591
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_626
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Jauković, Marko",
year = "2019",
abstract = "Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia",
title = "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds",
pages = "580-591",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_626"
}

Krnjaja, V., Stanković, S., Obradović, A., Petrović, T., Mandić, V., Bijelić, Z.,& Jauković, M.. (2019). The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun., 580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626

Krnjaja V, Stanković S, Obradović A, Petrović T, Mandić V, Bijelić Z, Jauković M. The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia. 2019;:580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Jauković, Marko, "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds" in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia (2019):580-591,
https://hdl.handle.net/21.15107/rcub_ristocar_626 .