TY  - CONF
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Lučev, Milica
AU  - Stanković, Slavica
PY  - 2024
UR  - http://r.istocar.bg.ac.rs/handle/123456789/986
AB  - The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.
PB  - University of Zagreb Faculty of Agriculture, Zagreb, Croatia
C3  - Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
T1  - Trichothecene chemotypes of Fusarium graminearum species complex in Serbia
SP  - 225
EP  - 225
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_986
ER  - 

@conference{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Lučev, Milica and Stanković, Slavica",
year = "2024",
abstract = "The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.",
publisher = "University of Zagreb Faculty of Agriculture, Zagreb, Croatia",
journal = "Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia",
title = "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia",
pages = "225-225",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_986"
}

Obradović, A., Stepanović, J., Krnjaja, V., Lučev, M.,& Stanković, S.. (2024). Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
University of Zagreb Faculty of Agriculture, Zagreb, Croatia., 225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986

Obradović A, Stepanović J, Krnjaja V, Lučev M, Stanković S. Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia. 2024;:225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986 .

Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Lučev, Milica, Stanković, Slavica, "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia" in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia (2024):225-225,
https://hdl.handle.net/21.15107/rcub_ristocar_986 .

TY  - CONF
AU  - Mandić, Violeta
AU  - Krnjaja, Vesna
AU  - Simić, Aleksandar
AU  - Petričević, Maja
AU  - Gogić, Marija
AU  - Brankov, Milan
AU  - Stanojković, Aleksandar
PY  - 2023-10-04
UR  - http://r.istocar.bg.ac.rs/handle/123456789/891
AB  - Maize is the top-produced crop in the world. Its chemical composition makes it suitable for human consumption, animal feed, and industrial uses. The total world production of maize in 2021 was 1210 billion tonnes from cultivating about 205 million ha. However, there are many limiting factors for cultivation, such as soil pH. Acidic soils are present on 30%–40% of the world's arable land, while more than 1 billion hectares are alkaline soils. It is important to choose genotypes of crops tolerant to high or low pH. Therefore, we conducted research in laboratory conditions on the influence of pH values (5, 6, 7, and 8) on seed germination and seedling growth of two maize Serbian hybrids ZP 4708 and ZP 5797. The hybrid ZP 4708 had higher germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), ShDW, and SVI have the highest values in treatment with optimal pH 7, and lowest in treatment pH 5. However, these parameters did not
differ significantly between the treatments pH 6 and pH 7.
C3  - Book of abstract 14th International symposium modern trends in livestock production
T1  - Dependence of maize germination and seedlings growth on pH
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_891
ER  - 

@conference{
author = "Mandić, Violeta and Krnjaja, Vesna and Simić, Aleksandar and Petričević, Maja and Gogić, Marija and Brankov, Milan and Stanojković, Aleksandar",
year = "2023-10-04",
abstract = "Maize is the top-produced crop in the world. Its chemical composition makes it suitable for human consumption, animal feed, and industrial uses. The total world production of maize in 2021 was 1210 billion tonnes from cultivating about 205 million ha. However, there are many limiting factors for cultivation, such as soil pH. Acidic soils are present on 30%–40% of the world's arable land, while more than 1 billion hectares are alkaline soils. It is important to choose genotypes of crops tolerant to high or low pH. Therefore, we conducted research in laboratory conditions on the influence of pH values (5, 6, 7, and 8) on seed germination and seedling growth of two maize Serbian hybrids ZP 4708 and ZP 5797. The hybrid ZP 4708 had higher germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), ShDW, and SVI have the highest values in treatment with optimal pH 7, and lowest in treatment pH 5. However, these parameters did not
differ significantly between the treatments pH 6 and pH 7.",
journal = "Book of abstract 14th International symposium modern trends in livestock production",
title = "Dependence of maize germination and seedlings growth on pH",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_891"
}

Mandić, V., Krnjaja, V., Simić, A., Petričević, M., Gogić, M., Brankov, M.,& Stanojković, A.. (2023-10-04). Dependence of maize germination and seedlings growth on pH. in Book of abstract 14th International symposium modern trends in livestock production.
https://hdl.handle.net/21.15107/rcub_ristocar_891

Mandić V, Krnjaja V, Simić A, Petričević M, Gogić M, Brankov M, Stanojković A. Dependence of maize germination and seedlings growth on pH. in Book of abstract 14th International symposium modern trends in livestock production. 2023;.
https://hdl.handle.net/21.15107/rcub_ristocar_891 .

Mandić, Violeta, Krnjaja, Vesna, Simić, Aleksandar, Petričević, Maja, Gogić, Marija, Brankov, Milan, Stanojković, Aleksandar, "Dependence of maize germination and seedlings growth on pH" in Book of abstract 14th International symposium modern trends in livestock production (2023-10-04),
https://hdl.handle.net/21.15107/rcub_ristocar_891 .

TY  - CONF
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/966
AB  - Vrste u okviru Fusarium graminearum kompleksa (Fg) su jedne od najvažnijih 
patogena strnih žita i kukuruza. Kompleks Fg sadrži najmanje 15 filogenetski različitih vrsta. 
Ove vrste prouzrokuju bolest poznatu kao fusarioza klasa pšenice, ječma i pirinča, kao i trulež 
klipa kukuruza. Pored toga što dovodi do gubitka prinosa, zaraženo zrno je lošijeg kvaliteta, 
uključujući kontaminaciju mikotoksinima od kojih su najznačajniji nivalenol (NIV), 
deoksinivalenol (DON) i njegove acetilovane forme (3ADON i 15ADON). U ovom istraživanju, 
ispitivana je patogenost izolata, pripadnost određenom hemotipu kao i potencijal za sintezu 
mikotoksina kod 22 izolata Fusarium graminearum sakupljenih sa zrna ječma na 12 lokaliteta 
u Srbiji. Određivanje hemotipa kao i potencijal sinteze mikotoksina urađen je pomoću tečne 
hromatografije sa tandem masenom spektrofotometrijom (HPLC/LC-MS). Proces 
prečišćavanja uzoraka je izvršen u koloni MycoSep 113 Trich i MycoSep 230 Niv (Romer Labs, 
USA). Kao mobilna faza korišćena je voda-acetonitril (90:10 v/v) za 3ADON i 15ADON. 
Komponente su razdvojene na Acclaim Polar Advantage II, C18 (150 × 4.6 mm, 3 μm) koloni 
na temperaturi od 25 oC. Patogenost ispitivanih izolata testirana je in vitro (u laboartoriji) na 
listu ječma. Veštačka inokulacija lista ječma obavljena je po metodi koju su opisali Imathiu i 
sar. (2009). Od 22 ispitivana izolata, dva izolata su pripadala 3ADON hemotipu, dok su svi 
ostali pripadali 15ADON hemotipu. Izolati sa 3ADON hemotipom su sintetisali niske 
koncentarcije ukupnog deoksinivalenola (4,12-11,09 µg/g), dok su izolati sa 15ADON 
hemotipom sintetisali veće koncentracije 8,74-102,32 µg/g. Rezultati u ovom radu su pokazali 
da između patogenosti izolata i sinteze mikotoksina u in vitro testu nije utvrđena značajna 
pozitivna korelacija (r = 0,32).
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.
T1  - Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma
SP  - 12
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_966
ER  - 

@conference{
author = "Obradović, Ana and Krnjaja, Vesna and Bulajić, Aleksandra and Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2023",
abstract = "Vrste u okviru Fusarium graminearum kompleksa (Fg) su jedne od najvažnijih 
patogena strnih žita i kukuruza. Kompleks Fg sadrži najmanje 15 filogenetski različitih vrsta. 
Ove vrste prouzrokuju bolest poznatu kao fusarioza klasa pšenice, ječma i pirinča, kao i trulež 
klipa kukuruza. Pored toga što dovodi do gubitka prinosa, zaraženo zrno je lošijeg kvaliteta, 
uključujući kontaminaciju mikotoksinima od kojih su najznačajniji nivalenol (NIV), 
deoksinivalenol (DON) i njegove acetilovane forme (3ADON i 15ADON). U ovom istraživanju, 
ispitivana je patogenost izolata, pripadnost određenom hemotipu kao i potencijal za sintezu 
mikotoksina kod 22 izolata Fusarium graminearum sakupljenih sa zrna ječma na 12 lokaliteta 
u Srbiji. Određivanje hemotipa kao i potencijal sinteze mikotoksina urađen je pomoću tečne 
hromatografije sa tandem masenom spektrofotometrijom (HPLC/LC-MS). Proces 
prečišćavanja uzoraka je izvršen u koloni MycoSep 113 Trich i MycoSep 230 Niv (Romer Labs, 
USA). Kao mobilna faza korišćena je voda-acetonitril (90:10 v/v) za 3ADON i 15ADON. 
Komponente su razdvojene na Acclaim Polar Advantage II, C18 (150 × 4.6 mm, 3 μm) koloni 
na temperaturi od 25 oC. Patogenost ispitivanih izolata testirana je in vitro (u laboartoriji) na 
listu ječma. Veštačka inokulacija lista ječma obavljena je po metodi koju su opisali Imathiu i 
sar. (2009). Od 22 ispitivana izolata, dva izolata su pripadala 3ADON hemotipu, dok su svi 
ostali pripadali 15ADON hemotipu. Izolati sa 3ADON hemotipom su sintetisali niske 
koncentarcije ukupnog deoksinivalenola (4,12-11,09 µg/g), dok su izolati sa 15ADON 
hemotipom sintetisali veće koncentracije 8,74-102,32 µg/g. Rezultati u ovom radu su pokazali 
da između patogenosti izolata i sinteze mikotoksina u in vitro testu nije utvrđena značajna 
pozitivna korelacija (r = 0,32).",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.",
title = "Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma",
pages = "12-12",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_966"
}

Obradović, A., Krnjaja, V., Bulajić, A., Nikolić, M., Savić, I.,& Stanković, S.. (2023). Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma. in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.
Društvo za zaštitu bilja Srbije., 12-12.
https://hdl.handle.net/21.15107/rcub_ristocar_966

Obradović A, Krnjaja V, Bulajić A, Nikolić M, Savić I, Stanković S. Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma. in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.. 2023;:12-12.
https://hdl.handle.net/21.15107/rcub_ristocar_966 .

Obradović, Ana, Krnjaja, Vesna, Bulajić, Aleksandra, Nikolić, Milica, Savić, Iva, Stanković, Slavica, "Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma" in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija. (2023):12-12,
https://hdl.handle.net/21.15107/rcub_ristocar_966 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/970
AB  - The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022
VL  - 39
IS  - 2
SP  - 205
EP  - 218
DO  - https://doi.org/10.2298/BAH2302205K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022",
volume = "39",
number = "2",
pages = "205-218",
doi = "https://doi.org/10.2298/BAH2302205K"
}

Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 39(2), 205-218.
https://doi.org/https://doi.org/10.2298/BAH2302205K

Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry. 2023;39(2):205-218.
doi:https://doi.org/10.2298/BAH2302205K .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022" in Biotechnology in Animal Husbandry, 39, no. 2 (2023):205-218,
https://doi.org/https://doi.org/10.2298/BAH2302205K . .

TY  - CONF
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Stanković, Goran
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/967
AB  - U ovom istraživanju je analizirana učestalost prisustva toksigenih vrsta gljiva 
na zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 
434, ZP 560, ZP 606, ZP 666). Uzorci su prikupljeni u vreme berbe, iz tri različita 
lokaliteta (Zemun Polje, Bečej, Šabac) u Srbiji. Koristeći standardne mikološke 
metode izolacije, istraživači su izolovali toksigene vrste gljiva. Dobijene kolonije 
su prečišćene kako bi se dobila čista kultura gljiva. Ove kulture su potom korišćene 
za identifikaciju različitih vrsta gljiva. Kulture gljiva su gajene na različitim podlogama kao što su krompir-dekstroza podloga (PDA), podloga sa fragmentima 
sterilnog lista karanfila (CLA) i sintetička podloga (SNA). Inkubacija kultura je 
obavljena pri kontrolisanoj temperaturi od 25±1ºC. Identifikacija izolovanih vrsta 
je izvršena prema Nelsonui sar. (1983). Statistička analiza prikupljenih podataka 
izvedena je korišćenjem analize varijanse (ANOVA), uz primenu paketa Statistica 
10 (StatSoft, Inc., SAD). Značajnost razlika između srednjih vrednosti parametara 
je ocenjena putem F-testa pri nivou značajnosti od P ≤ 0,05. Tokom mikoloških 
istraživanja zrna kukuruza, potvrđeno je prisustvo toksigenih gljiva iz tri roda: 
Aspergillus, Fusarium i Penicillium. U lokalitetima koji su obuhvaćeni istraživanjem, gljiva Fusarium verticillioides je bila najčešće izolovana, sa maksimalnom 
učestalošću od 36% kod većine ispitivanih hibrida. Prisustvo Aspergillus spp. 
se kretalo od 0 do 19%, dok je prisustvo Penicillium spp. variralo od 0 do 25%.
Imajući u vidu povoljne agroekološke uslove u Srbiji za razvoj toksigenih gljiva i 
njihovih mikotoksina, neophodno je redovno sprovoditi kontrolu nad sadržajem 
mikotoksina u zrnu kukuruza, kako tokom žetve tako i nakon nje.
PB  - Društvo genetičara Srbije
PB  - Društvo selekcionera i semenara Republike Srbije
C3  - Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.
T1  - Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_967
ER  - 

@conference{
author = "Obradović, Ana and Krnjaja, Vesna and Stanković, Goran and Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2023",
abstract = "U ovom istraživanju je analizirana učestalost prisustva toksigenih vrsta gljiva 
na zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 
434, ZP 560, ZP 606, ZP 666). Uzorci su prikupljeni u vreme berbe, iz tri različita 
lokaliteta (Zemun Polje, Bečej, Šabac) u Srbiji. Koristeći standardne mikološke 
metode izolacije, istraživači su izolovali toksigene vrste gljiva. Dobijene kolonije 
su prečišćene kako bi se dobila čista kultura gljiva. Ove kulture su potom korišćene 
za identifikaciju različitih vrsta gljiva. Kulture gljiva su gajene na različitim podlogama kao što su krompir-dekstroza podloga (PDA), podloga sa fragmentima 
sterilnog lista karanfila (CLA) i sintetička podloga (SNA). Inkubacija kultura je 
obavljena pri kontrolisanoj temperaturi od 25±1ºC. Identifikacija izolovanih vrsta 
je izvršena prema Nelsonui sar. (1983). Statistička analiza prikupljenih podataka 
izvedena je korišćenjem analize varijanse (ANOVA), uz primenu paketa Statistica 
10 (StatSoft, Inc., SAD). Značajnost razlika između srednjih vrednosti parametara 
je ocenjena putem F-testa pri nivou značajnosti od P ≤ 0,05. Tokom mikoloških 
istraživanja zrna kukuruza, potvrđeno je prisustvo toksigenih gljiva iz tri roda: 
Aspergillus, Fusarium i Penicillium. U lokalitetima koji su obuhvaćeni istraživanjem, gljiva Fusarium verticillioides je bila najčešće izolovana, sa maksimalnom 
učestalošću od 36% kod većine ispitivanih hibrida. Prisustvo Aspergillus spp. 
se kretalo od 0 do 19%, dok je prisustvo Penicillium spp. variralo od 0 do 25%.
Imajući u vidu povoljne agroekološke uslove u Srbiji za razvoj toksigenih gljiva i 
njihovih mikotoksina, neophodno je redovno sprovoditi kontrolu nad sadržajem 
mikotoksina u zrnu kukuruza, kako tokom žetve tako i nakon nje.",
publisher = "Društvo genetičara Srbije, Društvo selekcionera i semenara Republike Srbije",
journal = "Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.",
title = "Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_967"
}

Obradović, A., Krnjaja, V., Stanković, G., Nikolić, M., Savić, I.,& Stanković, S.. (2023). Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji. in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.
Društvo genetičara Srbije., 105-105.
https://hdl.handle.net/21.15107/rcub_ristocar_967

Obradović A, Krnjaja V, Stanković G, Nikolić M, Savić I, Stanković S. Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji. in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.. 2023;:105-105.
https://hdl.handle.net/21.15107/rcub_ristocar_967 .

Obradović, Ana, Krnjaja, Vesna, Stanković, Goran, Nikolić, Milica, Savić, Iva, Stanković, Slavica, "Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji" in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija. (2023):105-105,
https://hdl.handle.net/21.15107/rcub_ristocar_967 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/905
AB  - Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_905
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_905"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):80-80,
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/903
AB  - Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 566
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_903
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "566-576",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_903"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. 2023;:566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia (2023):566-576,
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

TY  - CONF
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/615
AB  - Увод: Од укупног пољопривредног земљишта у Србији око 4,6% су слана и алкална земљишта. Из тог разлога је непходно пратити толерантност биљних врста према соном стресу. Циљ овог истраживања је био да  се испита ефекат соног стреса на клијање и параметре клијанаца две домаће сорте црвене детелине.
Материјал и метод рада: Огледи су спроведени у у лабораторији Института за сточарство 2017. године. Анализиране су две домаће сорте црвене детелине К-17 и К-39. Семена су стерилисана 2% NaOCl, темељно испрана пет пута млазом дестиловане воде, осушена, помешана у авану са кварцним песком и протрљана ради механичког повређивања семењаче. У стерилисаним пластичним судовима постављено је по сто семена на филтер папиру предходно навлаженом са 10 мл одговарајуће концентрације соли (0, 50, 100, 150 и 200 mM NaCl) у 4 понављања. Судови су стављени у мрак на температури 20±1°C. Након 4 дана утврђена је енергија клијања, а након 7 дана клијавост. Дужина корена и хипокотила мерени су након десет дана на 30 насумично одабраних клијанаца у сваком третману и репликацији. Вигор индекс (ВИ) је израчунат: ВИ (%) = (дужина корена + дужина хипокотила) ×
% клијавости. Експеримент је постављен по рандомизираном блок систему. Добијени подаци су статистички обрађени методом анализе варијансе у програму Статистика 10. Такијев тест је коришћен за поређење средњих вредности на нивоу значајности p≤0.05.
Резултати: Сорта К-17 имала је већу енергију клијања, клијавост и вигор индекс него сорта К-32. Вредности испитиваних параметара се нису разликовале између 0 и 50 mM NaCl. Даље повећање концентрације соли у подлози за наклијавање (≥ 100 mM NaCl) статистички је значајно смањило вредности истраживаних параметара.
Закључак: Испитиване комерцијалне сорте црвене детелине показују осељивост на високу концентрацију соли, иако сорта К-17 има већу енергију клијања, клијавост и вигор индекс него сорта К-32.
PB  - Друштво за крмно биље Републике Србије
C3  - ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије
T1  - Утицај сорте и соног стреса на почетни пораст црвене детелине
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_615
ER  - 

@conference{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna",
year = "2019",
abstract = "Увод: Од укупног пољопривредног земљишта у Србији око 4,6% су слана и алкална земљишта. Из тог разлога је непходно пратити толерантност биљних врста према соном стресу. Циљ овог истраживања је био да  се испита ефекат соног стреса на клијање и параметре клијанаца две домаће сорте црвене детелине.
Материјал и метод рада: Огледи су спроведени у у лабораторији Института за сточарство 2017. године. Анализиране су две домаће сорте црвене детелине К-17 и К-39. Семена су стерилисана 2% NaOCl, темељно испрана пет пута млазом дестиловане воде, осушена, помешана у авану са кварцним песком и протрљана ради механичког повређивања семењаче. У стерилисаним пластичним судовима постављено је по сто семена на филтер папиру предходно навлаженом са 10 мл одговарајуће концентрације соли (0, 50, 100, 150 и 200 mM NaCl) у 4 понављања. Судови су стављени у мрак на температури 20±1°C. Након 4 дана утврђена је енергија клијања, а након 7 дана клијавост. Дужина корена и хипокотила мерени су након десет дана на 30 насумично одабраних клијанаца у сваком третману и репликацији. Вигор индекс (ВИ) је израчунат: ВИ (%) = (дужина корена + дужина хипокотила) ×
% клијавости. Експеримент је постављен по рандомизираном блок систему. Добијени подаци су статистички обрађени методом анализе варијансе у програму Статистика 10. Такијев тест је коришћен за поређење средњих вредности на нивоу значајности p≤0.05.
Резултати: Сорта К-17 имала је већу енергију клијања, клијавост и вигор индекс него сорта К-32. Вредности испитиваних параметара се нису разликовале између 0 и 50 mM NaCl. Даље повећање концентрације соли у подлози за наклијавање (≥ 100 mM NaCl) статистички је значајно смањило вредности истраживаних параметара.
Закључак: Испитиване комерцијалне сорте црвене детелине показују осељивост на високу концентрацију соли, иако сорта К-17 има већу енергију клијања, клијавост и вигор индекс него сорта К-32.",
publisher = "Друштво за крмно биље Републике Србије",
journal = "ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије",
title = "Утицај сорте и соног стреса на почетни пораст црвене детелине",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_615"
}

Mandić, V., Bijelić, Z.,& Krnjaja, V.. (2019). Утицај сорте и соног стреса на почетни пораст црвене детелине. in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије
Друштво за крмно биље Републике Србије., 47-48.
https://hdl.handle.net/21.15107/rcub_ristocar_615

Mandić V, Bijelić Z, Krnjaja V. Утицај сорте и соног стреса на почетни пораст црвене детелине. in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије. 2019;:47-48.
https://hdl.handle.net/21.15107/rcub_ristocar_615 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, "Утицај сорте и соног стреса на почетни пораст црвене детелине" in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије (2019):47-48,
https://hdl.handle.net/21.15107/rcub_ristocar_615 .