TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/905
AB  - Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_905
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_905"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):80-80,
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/903
AB  - Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 566
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_903
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "566-576",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_903"
}

Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903

Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. 2023;:566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia (2023):566-576,
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Krnjaja, Vesna
AU  - Živković, Sanja
AU  - Trkulja, Vojislav
PY  - 2021-05
UR  - http://r.istocar.bg.ac.rs/handle/123456789/742
AB  - In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.
PB  - Verlag Eugen Ulmer GmbH, Germany
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia
DO  - 10.1007/s41348-021-00476-5
ER  - 

@article{
author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Živković, Sanja and Trkulja, Vojislav",
year = "2021-05",
abstract = "In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.",
publisher = "Verlag Eugen Ulmer GmbH, Germany",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia",
doi = "10.1007/s41348-021-00476-5"
}

Vasić, T., Jevremović, D., Krnjaja, V., Živković, S.,& Trkulja, V.. (2021-05). Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection
Verlag Eugen Ulmer GmbH, Germany..
https://doi.org/10.1007/s41348-021-00476-5

Vasić T, Jevremović D, Krnjaja V, Živković S, Trkulja V. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection. 2021;.
doi:10.1007/s41348-021-00476-5 .

Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Živković, Sanja, Trkulja, Vojislav, "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia" in Journal of Plant Diseases and Protection (2021-05),
https://doi.org/10.1007/s41348-021-00476-5 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Radović, Čedomir
PY  - 2021-02-25
UR  - http://r.istocar.bg.ac.rs/handle/123456789/730
AB  - The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.
T2  - Crop Protection
T1  - Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels
VL  - 144
SP  - 105601
DO  - https://doi.org/10.1016/j.cropro.2021.105601
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Radović, Čedomir",
year = "2021-02-25",
abstract = "The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.",
journal = "Crop Protection",
title = "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels",
volume = "144",
pages = "105601",
doi = "https://doi.org/10.1016/j.cropro.2021.105601"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Radović, Č.. (2021-02-25). Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection, 144, 105601.
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Vasić T, Radović Č. Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection. 2021;144:105601.
doi:https://doi.org/10.1016/j.cropro.2021.105601 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Radović, Čedomir, "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels" in Crop Protection, 144 (2021-02-25):105601,
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601 . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Nikolić, Milica
AU  - Vasić, Tanja
AU  - Delić, Nikola
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/738
AB  - The aim of this study was to evaluate fungal infection, with a 
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a 
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian 
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest 
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of 
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F. 
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in 
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in 
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of 
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13) 
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars 
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A 
statistically significant difference in DON level was found between the two tested 
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to 
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant 
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in 
the tested seed of Italian ryegrass indicate on potentially significant participation of 
DON in FHB pathogenesis, as well as a potential risk for the quality seed 
production, feed safety and the food chain in general. In Serbia, this is the first 
report about Fusarium infection and DON presence in ryegrass seed.
C3  - Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631
T1  - Fusarium spp. and deoxynivalenol contamination of ryegrass seeds
SP  - 622
EP  - 631
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_738
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Nikolić, Milica and Vasić, Tanja and Delić, Nikola",
year = "2021",
abstract = "The aim of this study was to evaluate fungal infection, with a 
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a 
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian 
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest 
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of 
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F. 
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in 
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in 
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of 
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13) 
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars 
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A 
statistically significant difference in DON level was found between the two tested 
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to 
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant 
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in 
the tested seed of Italian ryegrass indicate on potentially significant participation of 
DON in FHB pathogenesis, as well as a potential risk for the quality seed 
production, feed safety and the food chain in general. In Serbia, this is the first 
report about Fusarium infection and DON presence in ryegrass seed.",
journal = "Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631",
title = "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds",
pages = "622-631",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_738"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Nikolić, M., Vasić, T.,& Delić, N.. (2021). Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631, 622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Nikolić M, Vasić T, Delić N. Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631. 2021;:622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Nikolić, Milica, Vasić, Tanja, Delić, Nikola, "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds" in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631 (2021):622-631,
https://hdl.handle.net/21.15107/rcub_ristocar_738 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica Ž.
AU  - Obradović, A.
AU  - Vasić, Tanja
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/568
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B1 (AFB1), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha−1 (PD1), 64,000 plants ha−1 (PD2) and 75,000 plants ha−1 (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P ≤ 0.01) at PD3. The effect of year was also significant (P ≤ 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins. © 2018 Elsevier Ltd
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica Ž. and Obradović, A. and Vasić, Tanja and Lukić, Miloš and Bijelić, Zorica",
year = "2019",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B1 (AFB1), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha−1 (PD1), 64,000 plants ha−1 (PD2) and 75,000 plants ha−1 (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P ≤ 0.01) at PD3. The effect of year was also significant (P ≤ 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins. © 2018 Elsevier Ltd",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}

Krnjaja, V., Mandić, V., Stanković, S. Ž., Obradović, A., Vasić, T., Lukić, M.,& Bijelić, Z.. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. in Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021

Krnjaja V, Mandić V, Stanković SŽ, Obradović A, Vasić T, Lukić M, Bijelić Z. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. in Crop Protection. 2019;116:126-131.
doi:10.1016/j.cropro.2018.10.021 .

Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica Ž., Obradović, A., Vasić, Tanja, Lukić, Miloš, Bijelić, Zorica, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" in Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 . .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Vojinović, Uroš
AU  - Žujović, Suzana
AU  - Krnjaja, Vesna
AU  - Živković, Sanja
AU  - Marković, Jordan
AU  - Stević, Milan
PY  - 2019
UR  - https://www.tandfonline.com/eprint/AB5HWK3AX3PVSGGPH3NQ/full?target=10.1080%2F03601234.2019.1653735&
UR  - http://r.istocar.bg.ac.rs/handle/123456789/623
AB  - Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azoxystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.
PB  - Taylor & Francis Inc.
T2  - Journal of Environmental Science and Health, Part B
T1  - In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum
DO  - DOI: 10.1080/03601234.2019.1653735
ER  - 

@article{
author = "Vasić, Tanja and Vojinović, Uroš and Žujović, Suzana and Krnjaja, Vesna and Živković, Sanja and Marković, Jordan and Stević, Milan",
year = "2019",
abstract = "Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azoxystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.",
publisher = "Taylor & Francis Inc.",
journal = "Journal of Environmental Science and Health, Part B",
title = "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum",
doi = "DOI: 10.1080/03601234.2019.1653735"
}

Vasić, T., Vojinović, U., Žujović, S., Krnjaja, V., Živković, S., Marković, J.,& Stević, M.. (2019). In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health, Part B
Taylor & Francis Inc...
https://doi.org/DOI: 10.1080/03601234.2019.1653735

Vasić T, Vojinović U, Žujović S, Krnjaja V, Živković S, Marković J, Stević M. In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health, Part B. 2019;.
doi:DOI: 10.1080/03601234.2019.1653735 .

Vasić, Tanja, Vojinović, Uroš, Žujović, Suzana, Krnjaja, Vesna, Živković, Sanja, Marković, Jordan, Stević, Milan, "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum" in Journal of Environmental Science and Health, Part B (2019),
https://doi.org/DOI: 10.1080/03601234.2019.1653735 . .

TY  - CONF
AU  - Vasić, Tanja
AU  - Krnjaja, Vesna
AU  - Marković, Jordan
AU  - Andjelković, Snežana
AU  - Petrović, Mirjana
AU  - Leposavić, Aleksandra
AU  - Terzić, Dragan
PY  - 2019
UR  - http://agrosym.ues.rs.ba/agrosym/agrosym_2019/BOOK_OF_PROCEEDINGS_2019_FINAL.pdf
UR  - http://r.istocar.bg.ac.rs/handle/123456789/630
AB  - Birdsfoot trefoil (Lotus corniculatus L.) is a species adapted to field conditions in the most important livestock production region in the all continents and constitutes a very valuable forage species. It has a high ability to utilize nutrients and has very modest demands on the conditions of its growth. The roots of birdsfoot trefoil are associated with bacteria that fix atmospheric nitrogen and, thusly, its populations increase the availability of nitrogen in the soil. This species often forms dense, fibrous root networks that reduce soil erosion. Isolation of the pathogen was done from the leaves and roots. Black leaf spots were observed on birdsfoot trefoil in field plots in Serbia. These spots were circular to irregular. Single lesions often coalesced to form larger lesions and became dark brown. On the roots systems of a large number of plants birdsfoot trefoil symptoms of light to dark brown necrosis and discoloration of conductive tissues were observed. There has not been a systematic research of birdsfoot trefoil mycoflora in Serbia. This research aims to present the results of preliminary research of mycopopulation of 12 different genotypes of birdsfoot trefoil. Total of 480 plant parts have been examined and 7 genera of fungi were isolated: Alternaria, Fusarium, Phythophthora, Mucor, Sclerotinia, Bipolaris and Rhizoctonia. Considering the importance of birdsfoot trefoil as a fodder crop in Serbia, the aim of this study was to identify phytopathogenic fungi as casual agents of diseases in birdsfoot trefoil for a clearer perception of problems (the extinction of plants, reducing yields, deterioration of the quality of feed and others) arising as a result of the presence of those fungi.
C3  - Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”
T1  - Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.
SP  - 1025
EP  - 1029
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_630
ER  - 

@conference{
author = "Vasić, Tanja and Krnjaja, Vesna and Marković, Jordan and Andjelković, Snežana and Petrović, Mirjana and Leposavić, Aleksandra and Terzić, Dragan",
year = "2019",
abstract = "Birdsfoot trefoil (Lotus corniculatus L.) is a species adapted to field conditions in the most important livestock production region in the all continents and constitutes a very valuable forage species. It has a high ability to utilize nutrients and has very modest demands on the conditions of its growth. The roots of birdsfoot trefoil are associated with bacteria that fix atmospheric nitrogen and, thusly, its populations increase the availability of nitrogen in the soil. This species often forms dense, fibrous root networks that reduce soil erosion. Isolation of the pathogen was done from the leaves and roots. Black leaf spots were observed on birdsfoot trefoil in field plots in Serbia. These spots were circular to irregular. Single lesions often coalesced to form larger lesions and became dark brown. On the roots systems of a large number of plants birdsfoot trefoil symptoms of light to dark brown necrosis and discoloration of conductive tissues were observed. There has not been a systematic research of birdsfoot trefoil mycoflora in Serbia. This research aims to present the results of preliminary research of mycopopulation of 12 different genotypes of birdsfoot trefoil. Total of 480 plant parts have been examined and 7 genera of fungi were isolated: Alternaria, Fusarium, Phythophthora, Mucor, Sclerotinia, Bipolaris and Rhizoctonia. Considering the importance of birdsfoot trefoil as a fodder crop in Serbia, the aim of this study was to identify phytopathogenic fungi as casual agents of diseases in birdsfoot trefoil for a clearer perception of problems (the extinction of plants, reducing yields, deterioration of the quality of feed and others) arising as a result of the presence of those fungi.",
journal = "Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”",
title = "Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.",
pages = "1025-1029",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_630"
}

Vasić, T., Krnjaja, V., Marković, J., Andjelković, S., Petrović, M., Leposavić, A.,& Terzić, D.. (2019). Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.. in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”, 1025-1029.
https://hdl.handle.net/21.15107/rcub_ristocar_630

Vasić T, Krnjaja V, Marković J, Andjelković S, Petrović M, Leposavić A, Terzić D. Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia.. in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019”. 2019;:1025-1029.
https://hdl.handle.net/21.15107/rcub_ristocar_630 .

Vasić, Tanja, Krnjaja, Vesna, Marković, Jordan, Andjelković, Snežana, Petrović, Mirjana, Leposavić, Aleksandra, Terzić, Dragan, "Fungal pathogens of birdsfoot trefoil (Lotus corniculatus L.) in Serbia." in Proceedings of X International Scientific Agricultural Symposium “Agrosim 2019” (2019):1025-1029,
https://hdl.handle.net/21.15107/rcub_ristocar_630 .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Krnjaja, Vesna
AU  - Leposavić, Aleksandar
AU  - Anđelković, S.
AU  - Živković, Sanja
AU  - Paunović, Svetlana
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/531
AB  - In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia
VL  - 15
IS  - 3
SP  - e10SC02
DO  - 10.5424/sjar/2017153-11297
ER  - 

@article{
author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Leposavić, Aleksandar and Anđelković, S. and Živković, Sanja and Paunović, Svetlana",
year = "2017",
abstract = "In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia",
volume = "15",
number = "3",
pages = "e10SC02",
doi = "10.5424/sjar/2017153-11297"
}

Vasić, T., Jevremović, D., Krnjaja, V., Leposavić, A., Anđelković, S., Živković, S.,& Paunović, S.. (2017). Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia. in Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 15(3), e10SC02.
https://doi.org/10.5424/sjar/2017153-11297

Vasić T, Jevremović D, Krnjaja V, Leposavić A, Anđelković S, Živković S, Paunović S. Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia. in Spanish Journal of Agricultural Research. 2017;15(3):e10SC02.
doi:10.5424/sjar/2017153-11297 .

Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Leposavić, Aleksandar, Anđelković, S., Živković, Sanja, Paunović, Svetlana, "Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia" in Spanish Journal of Agricultural Research, 15, no. 3 (2017):e10SC02,
https://doi.org/10.5424/sjar/2017153-11297 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica Ž.
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko M.
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/503
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica Ž. and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko M.",
year = "2016",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}

Krnjaja, V., Stanković, S. Ž., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. M.. (2016). Fungal and mycotoxin contamination of maize hybrids in different maturity groups. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K

Krnjaja V, Stanković SŽ, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković MM. Fungal and mycotoxin contamination of maize hybrids in different maturity groups. in Biotechnology in Animal Husbandry. 2016;32(1):71-81.
doi:10.2298/BAH1601071K .

Krnjaja, Vesna, Stanković, Slavica Ž., Obradović, Ana, Mandić, Violeta, Bijelić, Zorica, Vasić, Tanja, Jauković, Marko M., "Fungal and mycotoxin contamination of maize hybrids in different maturity groups" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K . .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Stanković, Slavica Ž.
AU  - Terzic, Dragan
AU  - Milenković, Jasmina
AU  - Marković, Jordan
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/484
AB  - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
PB  - Serbian Genetics Society, Belgrade
T2  - Genetika-Belgrade
T1  - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
VL  - 48
IS  - 1
SP  - 187
EP  - 198
DO  - 10.2298/GENSR1601187V
ER  - 

@article{
author = "Vasić, Tanja and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica Ž. and Terzic, Dragan and Milenković, Jasmina and Marković, Jordan",
year = "2016",
abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.",
publisher = "Serbian Genetics Society, Belgrade",
journal = "Genetika-Belgrade",
title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover",
volume = "48",
number = "1",
pages = "187-198",
doi = "10.2298/GENSR1601187V"
}

Vasić, T., Krnjaja, V., Jevremović, D., Stanković, S. Ž., Terzic, D., Milenković, J.,& Marković, J.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade
Serbian Genetics Society, Belgrade., 48(1), 187-198.
https://doi.org/10.2298/GENSR1601187V

Vasić T, Krnjaja V, Jevremović D, Stanković SŽ, Terzic D, Milenković J, Marković J. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade. 2016;48(1):187-198.
doi:10.2298/GENSR1601187V .

Vasić, Tanja, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica Ž., Terzic, Dragan, Milenković, Jasmina, Marković, Jordan, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika-Belgrade, 48, no. 1 (2016):187-198,
https://doi.org/10.2298/GENSR1601187V . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Vasić, Tanja
AU  - Obradović, Ana
PY  - 2015
UR  - http://r.istocar.bg.ac.rs/handle/123456789/453
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  (lt) = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  (lt) = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON. (C) 2014 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Vasić, Tanja and Obradović, Ana",
year = "2015",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  (lt) = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  (lt) = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON. (C) 2014 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}

Krnjaja, V., Mandić, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Vasić, T.,& Obradović, A.. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001

Krnjaja V, Mandić V, Lević J, Stanković SŽ, Petrović TS, Vasić T, Obradović A. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection. 2015;67:251-256.
doi:10.1016/j.cropro.2014.11.001 .

Krnjaja, Vesna, Mandić, Violeta, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Vasić, Tanja, Obradović, Ana, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" in Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 . .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Bulajić, Aleksandra
AU  - Krnjaja, Vesna
AU  - Jevremović, Darko
AU  - Živković, Sanja
AU  - Anđelković, Bojan S.
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/425
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Anthracnose on Alfalfa Caused by Colletotrichum linicola in Serbia
VL  - 98
IS  - 9
SP  - 1276
EP  - 1276
DO  - 10.1094/PDIS-03-14-0258-PDN
ER  - 

@article{
author = "Vasić, Tanja and Bulajić, Aleksandra and Krnjaja, Vesna and Jevremović, Darko and Živković, Sanja and Anđelković, Bojan S.",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Anthracnose on Alfalfa Caused by Colletotrichum linicola in Serbia",
volume = "98",
number = "9",
pages = "1276-1276",
doi = "10.1094/PDIS-03-14-0258-PDN"
}

Vasić, T., Bulajić, A., Krnjaja, V., Jevremović, D., Živković, S.,& Anđelković, B. S.. (2014). First Report of Anthracnose on Alfalfa Caused by Colletotrichum linicola in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1276-1276.
https://doi.org/10.1094/PDIS-03-14-0258-PDN

Vasić T, Bulajić A, Krnjaja V, Jevremović D, Živković S, Anđelković BS. First Report of Anthracnose on Alfalfa Caused by Colletotrichum linicola in Serbia. in Plant Disease. 2014;98(9):1276-1276.
doi:10.1094/PDIS-03-14-0258-PDN .

Vasić, Tanja, Bulajić, Aleksandra, Krnjaja, Vesna, Jevremović, Darko, Živković, Sanja, Anđelković, Bojan S., "First Report of Anthracnose on Alfalfa Caused by Colletotrichum linicola in Serbia" in Plant Disease, 98, no. 9 (2014):1276-1276,
https://doi.org/10.1094/PDIS-03-14-0258-PDN . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Vasić, Tanja
PY  - 2013
UR  - http://r.istocar.bg.ac.rs/handle/123456789/374
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Vasić, Tanja",
year = "2013",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž.,& Vasić, T.. (2013). The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K

Krnjaja V, Lević J, Stanković SŽ, Vasić T. The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi. in Pesticidi i fitomedicina. 2013;28(3):157-165.
doi:10.2298/PIF1303157K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Vasić, Tanja, "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi" in Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K . .

TY  - JOUR
AU  - Živković, Sanja
AU  - Vasić, Tanja
AU  - Trkulja, Vojislav
AU  - Krnjaja, Vesna
AU  - Marković, Jordan
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/314
AB  - Pathogenicity of four isolates of Eutypa lata originating from Serbia (VL 17, VL 27, VL 29, VL 30) and two French reference isolates (8F and BX 1.10), were examined and compared on unrooted cuttings of Cabernet Sauvignon. Additionally, susceptibility to infection by all six isolates of E. lata was tested on unrooted cuttings of six different grapevine cultivars including Riesling white, Opuzenska rana, Sauvignon, Radmilovacki muskat, Beogradska besemena and Negotinski rubin in greenhouse experiments. All tested isolates proved to be pathogenic on Cabernet Sauvignon. Moreover, each grapevine cultivar showed different degrees of susceptibility to infection depending on the E. lata isolate. The effect of different culture media (PDA, MA, GWA, YA, WA and TA) on both sporulation of E. lata isolates and germination of conidia was also studied. Sporulation occurred after 30 days in three media (PDA, MA, GWA) and after 2 months on WA. No sporulation was observed on YA and TA. The ability of conidia to germinate was assessed on PDA, MA, GWA and WA under continuous UV light. Calculation revealed that the average percentage germination of conidia was very low 0,15%.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia
VL  - 17
IS  - 3
SP  - 7379
EP  - 7388
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_314
ER  - 

@article{
author = "Živković, Sanja and Vasić, Tanja and Trkulja, Vojislav and Krnjaja, Vesna and Marković, Jordan",
year = "2012",
abstract = "Pathogenicity of four isolates of Eutypa lata originating from Serbia (VL 17, VL 27, VL 29, VL 30) and two French reference isolates (8F and BX 1.10), were examined and compared on unrooted cuttings of Cabernet Sauvignon. Additionally, susceptibility to infection by all six isolates of E. lata was tested on unrooted cuttings of six different grapevine cultivars including Riesling white, Opuzenska rana, Sauvignon, Radmilovacki muskat, Beogradska besemena and Negotinski rubin in greenhouse experiments. All tested isolates proved to be pathogenic on Cabernet Sauvignon. Moreover, each grapevine cultivar showed different degrees of susceptibility to infection depending on the E. lata isolate. The effect of different culture media (PDA, MA, GWA, YA, WA and TA) on both sporulation of E. lata isolates and germination of conidia was also studied. Sporulation occurred after 30 days in three media (PDA, MA, GWA) and after 2 months on WA. No sporulation was observed on YA and TA. The ability of conidia to germinate was assessed on PDA, MA, GWA and WA under continuous UV light. Calculation revealed that the average percentage germination of conidia was very low 0,15%.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia",
volume = "17",
number = "3",
pages = "7379-7388",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_314"
}

Živković, S., Vasić, T., Trkulja, V., Krnjaja, V.,& Marković, J.. (2012). Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 17(3), 7379-7388.
https://hdl.handle.net/21.15107/rcub_ristocar_314

Živković S, Vasić T, Trkulja V, Krnjaja V, Marković J. Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia. in Romanian Biotechnological Letters. 2012;17(3):7379-7388.
https://hdl.handle.net/21.15107/rcub_ristocar_314 .

Živković, Sanja, Vasić, Tanja, Trkulja, Vojislav, Krnjaja, Vesna, Marković, Jordan, "Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia" in Romanian Biotechnological Letters, 17, no. 3 (2012):7379-7388,
https://hdl.handle.net/21.15107/rcub_ristocar_314 .