Obradović, Ana

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  • Obradović, Ana (23)
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Author's Bibliography

Trichothecene chemotypes of Fusarium graminearum species complex in Serbia

Obradović, Ana; Stepanović, Jelena; Krnjaja, Vesna; Lučev, Milica; Stanković, Slavica

(University of Zagreb Faculty of Agriculture, Zagreb, Croatia, 2024)

TY  - CONF
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Lučev, Milica
AU  - Stanković, Slavica
PY  - 2024
UR  - http://r.istocar.bg.ac.rs/handle/123456789/986
AB  - The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.
PB  - University of Zagreb Faculty of Agriculture, Zagreb, Croatia
C3  - Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
T1  - Trichothecene chemotypes of Fusarium graminearum species complex in Serbia
SP  - 225
EP  - 225
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_986
ER  - 
@conference{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Lučev, Milica and Stanković, Slavica",
year = "2024",
abstract = "The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.",
publisher = "University of Zagreb Faculty of Agriculture, Zagreb, Croatia",
journal = "Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia",
title = "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia",
pages = "225-225",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_986"
}
Obradović, A., Stepanović, J., Krnjaja, V., Lučev, M.,& Stanković, S.. (2024). Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
University of Zagreb Faculty of Agriculture, Zagreb, Croatia., 225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986
Obradović A, Stepanović J, Krnjaja V, Lučev M, Stanković S. Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia. 2024;:225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986 .
Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Lučev, Milica, Stanković, Slavica, "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia" in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia (2024):225-225,
https://hdl.handle.net/21.15107/rcub_ristocar_986 .

Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma

Obradović, Ana; Krnjaja, Vesna; Bulajić, Aleksandra; Nikolić, Milica; Savić, Iva; Stanković, Slavica

(Društvo za zaštitu bilja Srbije, 2023)

TY  - CONF
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/966
AB  - Vrste u okviru Fusarium graminearum kompleksa (Fg) su jedne od najvažnijih 
patogena strnih žita i kukuruza. Kompleks Fg sadrži najmanje 15 filogenetski različitih vrsta. 
Ove vrste prouzrokuju bolest poznatu kao fusarioza klasa pšenice, ječma i pirinča, kao i trulež 
klipa kukuruza. Pored toga što dovodi do gubitka prinosa, zaraženo zrno je lošijeg kvaliteta, 
uključujući kontaminaciju mikotoksinima od kojih su najznačajniji nivalenol (NIV), 
deoksinivalenol (DON) i njegove acetilovane forme (3ADON i 15ADON). U ovom istraživanju, 
ispitivana je patogenost izolata, pripadnost određenom hemotipu kao i potencijal za sintezu 
mikotoksina kod 22 izolata Fusarium graminearum sakupljenih sa zrna ječma na 12 lokaliteta 
u Srbiji. Određivanje hemotipa kao i potencijal sinteze mikotoksina urađen je pomoću tečne 
hromatografije sa tandem masenom spektrofotometrijom (HPLC/LC-MS). Proces 
prečišćavanja uzoraka je izvršen u koloni MycoSep 113 Trich i MycoSep 230 Niv (Romer Labs, 
USA). Kao mobilna faza korišćena je voda-acetonitril (90:10 v/v) za 3ADON i 15ADON. 
Komponente su razdvojene na Acclaim Polar Advantage II, C18 (150 × 4.6 mm, 3 μm) koloni 
na temperaturi od 25 oC. Patogenost ispitivanih izolata testirana je in vitro (u laboartoriji) na 
listu ječma. Veštačka inokulacija lista ječma obavljena je po metodi koju su opisali Imathiu i 
sar. (2009). Od 22 ispitivana izolata, dva izolata su pripadala 3ADON hemotipu, dok su svi 
ostali pripadali 15ADON hemotipu. Izolati sa 3ADON hemotipom su sintetisali niske 
koncentarcije ukupnog deoksinivalenola (4,12-11,09 µg/g), dok su izolati sa 15ADON 
hemotipom sintetisali veće koncentracije 8,74-102,32 µg/g. Rezultati u ovom radu su pokazali 
da između patogenosti izolata i sinteze mikotoksina u in vitro testu nije utvrđena značajna 
pozitivna korelacija (r = 0,32).
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.
T1  - Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma
SP  - 12
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_966
ER  - 
@conference{
author = "Obradović, Ana and Krnjaja, Vesna and Bulajić, Aleksandra and Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2023",
abstract = "Vrste u okviru Fusarium graminearum kompleksa (Fg) su jedne od najvažnijih 
patogena strnih žita i kukuruza. Kompleks Fg sadrži najmanje 15 filogenetski različitih vrsta. 
Ove vrste prouzrokuju bolest poznatu kao fusarioza klasa pšenice, ječma i pirinča, kao i trulež 
klipa kukuruza. Pored toga što dovodi do gubitka prinosa, zaraženo zrno je lošijeg kvaliteta, 
uključujući kontaminaciju mikotoksinima od kojih su najznačajniji nivalenol (NIV), 
deoksinivalenol (DON) i njegove acetilovane forme (3ADON i 15ADON). U ovom istraživanju, 
ispitivana je patogenost izolata, pripadnost određenom hemotipu kao i potencijal za sintezu 
mikotoksina kod 22 izolata Fusarium graminearum sakupljenih sa zrna ječma na 12 lokaliteta 
u Srbiji. Određivanje hemotipa kao i potencijal sinteze mikotoksina urađen je pomoću tečne 
hromatografije sa tandem masenom spektrofotometrijom (HPLC/LC-MS). Proces 
prečišćavanja uzoraka je izvršen u koloni MycoSep 113 Trich i MycoSep 230 Niv (Romer Labs, 
USA). Kao mobilna faza korišćena je voda-acetonitril (90:10 v/v) za 3ADON i 15ADON. 
Komponente su razdvojene na Acclaim Polar Advantage II, C18 (150 × 4.6 mm, 3 μm) koloni 
na temperaturi od 25 oC. Patogenost ispitivanih izolata testirana je in vitro (u laboartoriji) na 
listu ječma. Veštačka inokulacija lista ječma obavljena je po metodi koju su opisali Imathiu i 
sar. (2009). Od 22 ispitivana izolata, dva izolata su pripadala 3ADON hemotipu, dok su svi 
ostali pripadali 15ADON hemotipu. Izolati sa 3ADON hemotipom su sintetisali niske 
koncentarcije ukupnog deoksinivalenola (4,12-11,09 µg/g), dok su izolati sa 15ADON 
hemotipom sintetisali veće koncentracije 8,74-102,32 µg/g. Rezultati u ovom radu su pokazali 
da između patogenosti izolata i sinteze mikotoksina u in vitro testu nije utvrđena značajna 
pozitivna korelacija (r = 0,32).",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.",
title = "Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma",
pages = "12-12",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_966"
}
Obradović, A., Krnjaja, V., Bulajić, A., Nikolić, M., Savić, I.,& Stanković, S.. (2023). Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma. in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.
Društvo za zaštitu bilja Srbije., 12-12.
https://hdl.handle.net/21.15107/rcub_ristocar_966
Obradović A, Krnjaja V, Bulajić A, Nikolić M, Savić I, Stanković S. Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma. in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija.. 2023;:12-12.
https://hdl.handle.net/21.15107/rcub_ristocar_966 .
Obradović, Ana, Krnjaja, Vesna, Bulajić, Aleksandra, Nikolić, Milica, Savić, Iva, Stanković, Slavica, "Diverzitet i patogenost različitih hemotipova Fusarium graminearum kompleksa poreklom sa zrna ječma" in Zbornik rezimea radova. XVII simpozijum o zaštiti bilja, Zlatibor, 27.- 30. 11. 2023. godine, Srbija. (2023):12-12,
https://hdl.handle.net/21.15107/rcub_ristocar_966 .

Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Petrović, Tanja; Stanišić, Nikola; Delić, Nikola

(Institute for Animal Husbandry, Belgrade-Zemun, 2023)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/970
AB  - The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022
VL  - 39
IS  - 2
SP  - 205
EP  - 218
DO  - https://doi.org/10.2298/BAH2302205K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "The aim of this study was to evaluate the occurrence of total 
aflatoxins (AFs) and type-B fumonisins (FBs) in 65 maize grain samples collected 
during harvest in 2018 (13 samples), 2019 (11 samples), 2020 (9 samples), 2021 
(14 samples) and 2022 (18 samples) from different locations in suburb of Belgrade 
(Republic of Serbia). The average levels of AFs and FBs in mycotoxin-positive 
samples were 5.43 and 2910 µg kg–1
(2018), 5.28 and 2710 µg kg–1
(2019), 2.35 
and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1
(2021) and 5.32 and 20310 µg 
kg–1
(2022), respectively. In 23.08% (2018), 18.18% (2019), 22.22% (2020), 
64.29% (2021) and 27.78% (2022) of maize samples, the co-occurrence of AFs and 
FBs was established. The maximum limits of 10 µg kg–1
for AFs in maize and 4000 
µg kg–1
for FBs in unprocessed maize prescribed by regulations of Serbia and the 
European Union were exceeded for AFs in 14.29% (2021) and 5.56% (2022) of 
maize samples and for FBs in 7.69% (2018), 66.67% (2020), 28.57% (2021) and 
41.67% (2022) of maize samples. Multiple linear regression analyses showed a 
statistically significant influence of climate factors (air temperature, relative 
humidity and total rainfall) in July-September (2018-2022) on FBs levels. These 
results indicate the need for continuous monitoring of the health status of harvested 
maize grains and risk assessment of the potential presence of mycotoxins in the 
food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022",
volume = "39",
number = "2",
pages = "205-218",
doi = "https://doi.org/10.2298/BAH2302205K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 39(2), 205-218.
https://doi.org/https://doi.org/10.2298/BAH2302205K
Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022. in Biotechnology in Animal Husbandry. 2023;39(2):205-218.
doi:https://doi.org/10.2298/BAH2302205K .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Occurrence of aflatoxins and fumonisins in maize grains harvested in the territory of Belgrade (R. Serbia) from 2018 to 2022" in Biotechnology in Animal Husbandry, 39, no. 2 (2023):205-218,
https://doi.org/https://doi.org/10.2298/BAH2302205K . .

Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji

Obradović, Ana; Krnjaja, Vesna; Stanković, Goran; Nikolić, Milica; Savić, Iva; Stanković, Slavica

(Društvo genetičara Srbije, 2023)

TY  - CONF
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Stanković, Goran
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/967
AB  - U ovom istraživanju je analizirana učestalost prisustva toksigenih vrsta gljiva 
na zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 
434, ZP 560, ZP 606, ZP 666). Uzorci su prikupljeni u vreme berbe, iz tri različita 
lokaliteta (Zemun Polje, Bečej, Šabac) u Srbiji. Koristeći standardne mikološke 
metode izolacije, istraživači su izolovali toksigene vrste gljiva. Dobijene kolonije 
su prečišćene kako bi se dobila čista kultura gljiva. Ove kulture su potom korišćene 
za identifikaciju različitih vrsta gljiva. Kulture gljiva su gajene na različitim podlogama kao što su krompir-dekstroza podloga (PDA), podloga sa fragmentima 
sterilnog lista karanfila (CLA) i sintetička podloga (SNA). Inkubacija kultura je 
obavljena pri kontrolisanoj temperaturi od 25±1ºC. Identifikacija izolovanih vrsta 
je izvršena prema Nelsonui sar. (1983). Statistička analiza prikupljenih podataka 
izvedena je korišćenjem analize varijanse (ANOVA), uz primenu paketa Statistica 
10 (StatSoft, Inc., SAD). Značajnost razlika između srednjih vrednosti parametara 
je ocenjena putem F-testa pri nivou značajnosti od P ≤ 0,05. Tokom mikoloških 
istraživanja zrna kukuruza, potvrđeno je prisustvo toksigenih gljiva iz tri roda: 
Aspergillus, Fusarium i Penicillium. U lokalitetima koji su obuhvaćeni istraživanjem, gljiva Fusarium verticillioides je bila najčešće izolovana, sa maksimalnom 
učestalošću od 36% kod većine ispitivanih hibrida. Prisustvo Aspergillus spp. 
se kretalo od 0 do 19%, dok je prisustvo Penicillium spp. variralo od 0 do 25%.
Imajući u vidu povoljne agroekološke uslove u Srbiji za razvoj toksigenih gljiva i 
njihovih mikotoksina, neophodno je redovno sprovoditi kontrolu nad sadržajem 
mikotoksina u zrnu kukuruza, kako tokom žetve tako i nakon nje.
PB  - Društvo genetičara Srbije
PB  - Društvo selekcionera i semenara Republike Srbije
C3  - Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.
T1  - Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_967
ER  - 
@conference{
author = "Obradović, Ana and Krnjaja, Vesna and Stanković, Goran and Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2023",
abstract = "U ovom istraživanju je analizirana učestalost prisustva toksigenih vrsta gljiva 
na zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 
434, ZP 560, ZP 606, ZP 666). Uzorci su prikupljeni u vreme berbe, iz tri različita 
lokaliteta (Zemun Polje, Bečej, Šabac) u Srbiji. Koristeći standardne mikološke 
metode izolacije, istraživači su izolovali toksigene vrste gljiva. Dobijene kolonije 
su prečišćene kako bi se dobila čista kultura gljiva. Ove kulture su potom korišćene 
za identifikaciju različitih vrsta gljiva. Kulture gljiva su gajene na različitim podlogama kao što su krompir-dekstroza podloga (PDA), podloga sa fragmentima 
sterilnog lista karanfila (CLA) i sintetička podloga (SNA). Inkubacija kultura je 
obavljena pri kontrolisanoj temperaturi od 25±1ºC. Identifikacija izolovanih vrsta 
je izvršena prema Nelsonui sar. (1983). Statistička analiza prikupljenih podataka 
izvedena je korišćenjem analize varijanse (ANOVA), uz primenu paketa Statistica 
10 (StatSoft, Inc., SAD). Značajnost razlika između srednjih vrednosti parametara 
je ocenjena putem F-testa pri nivou značajnosti od P ≤ 0,05. Tokom mikoloških 
istraživanja zrna kukuruza, potvrđeno je prisustvo toksigenih gljiva iz tri roda: 
Aspergillus, Fusarium i Penicillium. U lokalitetima koji su obuhvaćeni istraživanjem, gljiva Fusarium verticillioides je bila najčešće izolovana, sa maksimalnom 
učestalošću od 36% kod većine ispitivanih hibrida. Prisustvo Aspergillus spp. 
se kretalo od 0 do 19%, dok je prisustvo Penicillium spp. variralo od 0 do 25%.
Imajući u vidu povoljne agroekološke uslove u Srbiji za razvoj toksigenih gljiva i 
njihovih mikotoksina, neophodno je redovno sprovoditi kontrolu nad sadržajem 
mikotoksina u zrnu kukuruza, kako tokom žetve tako i nakon nje.",
publisher = "Društvo genetičara Srbije, Društvo selekcionera i semenara Republike Srbije",
journal = "Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.",
title = "Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_967"
}
Obradović, A., Krnjaja, V., Stanković, G., Nikolić, M., Savić, I.,& Stanković, S.. (2023). Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji. in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.
Društvo genetičara Srbije., 105-105.
https://hdl.handle.net/21.15107/rcub_ristocar_967
Obradović A, Krnjaja V, Stanković G, Nikolić M, Savić I, Stanković S. Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji. in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija.. 2023;:105-105.
https://hdl.handle.net/21.15107/rcub_ristocar_967 .
Obradović, Ana, Krnjaja, Vesna, Stanković, Goran, Nikolić, Milica, Savić, Iva, Stanković, Slavica, "Učestalost toksigenih vrsta gljiva na zrnu hibrida kukuruza različite FAO grupe zrenja u Srbiji" in Zbornik apstrakata X simpozijuma Društva selekcionera i semenara Republike Srbije i VII simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbije. 16.-18. oktobar 2023. godine, Vrnjačka Banja, Srbija. (2023):105-105,
https://hdl.handle.net/21.15107/rcub_ristocar_967 .

Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar

Krnjaja, Vesna; Mandić, Violeta; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja; Lazarević, Marina

(Institute for Animal Husbandry, Belgrade-Zemun, Serbia, 2023)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/905
AB  - Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_905
ER  - 
@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main source of carbohydrates, fibre, 
proteins, vitamins, minerals and phytochemicals for human consumption. The nutrients from wheat kernels 
can also be used as livestock feed. Fusarium head blight (FHB), caused by fungal species of the Fusarium
genus, is one of the most important wheat diseases worldwide. The lack of FHB management strategies 
results in significant economic losses in yield and quality of wheat kernels. In this study, the influence of 
growing season and wheat cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 2015, was investigated. 
Significant influence of growing season (year) and wheat cultivar on disease (FHB index – FHBI, incidence 
of Fusarium-damaged kernels – FDK and levels of DON) and yield parameters (spike weight – SW, kernel 
weight per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were higher in 2014, 
while SW, KWS, and 100-kernel weight were lower in 2014 than in 2015. The mid-early wheat cultivar 
Simonida had lower FHBI, FDK, and levels of DON and significantly higher SW, KWS, and 100-kernel 
weight than the mid-late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_905"
}
Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905
Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:80-80.
https://hdl.handle.net/21.15107/rcub_ristocar_905 .
Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):80-80,
https://hdl.handle.net/21.15107/rcub_ristocar_905 .

Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Petrović, Tanja; Stanišić, Nikola; Delić, Nikola

(Institute for Animal Husbandry, Belgrade-Zemun, Serbia, 2023)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/904
AB  - Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_904
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_904"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904
Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):79-79,
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar

Krnjaja, Vesna; Mandić, Violeta; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja; Lazarević, Marina

(Institute for Animal Husbandry, Belgrade-Zemun, Serbia, 2023)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Lazarević, Marina
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/903
AB  - Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
T1  - Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar
SP  - 566
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_903
ER  - 
@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Lazarević, Marina",
year = "2023",
abstract = "Wheat is the most important staple food in the world and the main 
source of carbohydrates, fibre, proteins, vitamins, minerals and phytochemicals for 
human consumption. The nutrients from wheat kernels can also be used as 
livestock feed. Fusarium head blight (FHB), caused by fungal species of the 
Fusarium genus, is one of the most important wheat diseases worldwide. The lack 
of FHB management strategies results in significant economic losses in yield and 
quality of wheat kernels. In this study, the influence of growing season and wheat 
cultivar on some FHB and yield component traits, as well as on the trichothecene 
mycotoxin deoxynivalenol (DON) levels during the harvest period in 2014 and 
2015, was investigated. Significant influence of growing season (year) and wheat 
cultivar on disease (FHB index – FHBI, incidence of Fusarium-damaged kernels – 
FDK and levels of DON) and yield parameters (spike weight – SW, kernel weight 
per spike – KWS, and 100-kernel weight) was found. FHBI, FDK, and DON were 
higher in 2014, while SW, KWS, and 100-kernel weight were lower in 2014 than 
in 2015. The mid-early wheat cultivar Simonida had lower FHBI, FDK, and levels 
of DON and significantly higher SW, KWS, and 100-kernel weight than the mid late cultivar NS 40S. There was a significant effect of year × cultivar interaction on 
FHBI, FDK and 100-kernel weight.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia",
title = "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar",
pages = "566-576",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_903"
}
Krnjaja, V., Mandić, V., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Lazarević, M.. (2023). Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903
Krnjaja V, Mandić V, Stanković S, Obradović A, Petrović T, Vasić T, Lazarević M. Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar. in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. 2023;:566-576.
https://hdl.handle.net/21.15107/rcub_ristocar_903 .
Krnjaja, Vesna, Mandić, Violeta, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Lazarević, Marina, "Fusarium and deoxynivalenol contamination of winter wheat depending on growing season and cultivar" in Proceedings of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia (2023):566-576,
https://hdl.handle.net/21.15107/rcub_ristocar_903 .

Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Caro Petrović, Violeta; Gogić, Marija

(2022-07-17)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Caro Petrović, Violeta
AU  - Gogić, Marija
PY  - 2022-07-17
UR  - http://r.istocar.bg.ac.rs/handle/123456789/792
AB  - The main aim of this research was to study the effect of two sowing times (early and late) on Fusarium and fumonisin contamination and some yield component traits of two maize hybrids from the FAO maturity groups 500 (ZP 560) and 600 (ZP 666) within a two-year growing season (2016–2017). F. verticillioides and F. subglutinans have been identified as Fusarium ear rot (FER) pathogens and potential producers of B-type fumonisins (FBs), with F. verticillioides as the predominant Fusarium species in both years. The incidence of F. verticillioides and FB levels were affected by sowing time and maize hybridity. With early sowing and the mid-maturity hybrid ZP 560, F. verticillioides and FB contamination were lower than with late sowing and the late-maturity hybrid ZP 666. Yield parameters also differed significantly between sowing time and maize hybrid treatments. Early sowing increased ear length (EL), number of grains per ear (NGE), grain weight per ear (GWE), and grain yield per hectare (GY). The late-maturity hybrid ZP 666 had higher yield component traits and GY than the mid-maturity hybrid ZP 560. EL, GWE, thousand-grain weight (TGW), and GY were affected by year. Interactions between sowing times and maize hybrids were highly significant (p ≤ 0.01) for FB level, GWE, and GY. The obtained results indicate the importance of applying early sowing to achieve high maize grain yields with lower contamination by F. verticillioides and FBs. Although mid-maturity hybrid ZP 560 and late-maturity hybrid ZP 666 showed significant differences in terms of levels of F. verticillioides and FB contamination, both were susceptible to F. verticillioides, with high FB levels. These results should be useful to breeders of maize hybrids to create genotypes more resistant to these fungal contaminants.
T2  - Agriculture
T1  - Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits
VL  - 12
IS  - 7
SP  - 1042
DO  - https://doi.org/10.3390/agriculture12071042
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Caro Petrović, Violeta and Gogić, Marija",
year = "2022-07-17",
abstract = "The main aim of this research was to study the effect of two sowing times (early and late) on Fusarium and fumonisin contamination and some yield component traits of two maize hybrids from the FAO maturity groups 500 (ZP 560) and 600 (ZP 666) within a two-year growing season (2016–2017). F. verticillioides and F. subglutinans have been identified as Fusarium ear rot (FER) pathogens and potential producers of B-type fumonisins (FBs), with F. verticillioides as the predominant Fusarium species in both years. The incidence of F. verticillioides and FB levels were affected by sowing time and maize hybridity. With early sowing and the mid-maturity hybrid ZP 560, F. verticillioides and FB contamination were lower than with late sowing and the late-maturity hybrid ZP 666. Yield parameters also differed significantly between sowing time and maize hybrid treatments. Early sowing increased ear length (EL), number of grains per ear (NGE), grain weight per ear (GWE), and grain yield per hectare (GY). The late-maturity hybrid ZP 666 had higher yield component traits and GY than the mid-maturity hybrid ZP 560. EL, GWE, thousand-grain weight (TGW), and GY were affected by year. Interactions between sowing times and maize hybrids were highly significant (p ≤ 0.01) for FB level, GWE, and GY. The obtained results indicate the importance of applying early sowing to achieve high maize grain yields with lower contamination by F. verticillioides and FBs. Although mid-maturity hybrid ZP 560 and late-maturity hybrid ZP 666 showed significant differences in terms of levels of F. verticillioides and FB contamination, both were susceptible to F. verticillioides, with high FB levels. These results should be useful to breeders of maize hybrids to create genotypes more resistant to these fungal contaminants.",
journal = "Agriculture",
title = "Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits",
volume = "12",
number = "7",
pages = "1042",
doi = "https://doi.org/10.3390/agriculture12071042"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Caro Petrović, V.,& Gogić, M.. (2022-07-17). Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits. in Agriculture, 12(7), 1042.
https://doi.org/https://doi.org/10.3390/agriculture12071042
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Caro Petrović V, Gogić M. Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits. in Agriculture. 2022;12(7):1042.
doi:https://doi.org/10.3390/agriculture12071042 .
Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Caro Petrović, Violeta, Gogić, Marija, "Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits" in Agriculture, 12, no. 7 (2022-07-17):1042,
https://doi.org/https://doi.org/10.3390/agriculture12071042 . .

Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Caro Petrović, Violeta; Ostojić Andrić, Dušica

(The Balkans Scientific Center of the Russian Academy of Natural Science, 2022)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Caro Petrović, Violeta
AU  - Ostojić Andrić, Dušica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/790
AB  - Fusarium head blight (FHB) is economically the most important wheat disease, 
causing yield and quality losses. Fungal species from the genus Fusarium are the 
main causative agents of FHB, with Fusarium graminearum as the predominant 
species. F. graminearum synthesizes more different secondary metabolites 
(mycotoxins). In wheat, the most studied mycotoxins are trichothecenes and 
zearalenone produced by F. graminearum. Trichothecene deoxynivalenol (DON) 
and its acetylated forms 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON) are the most detected in wheat grains. Strategies for 
controlling FHB and DON in wheat includе different preharvest and postharvest 
measures, emphasizing integrated approaches. The main aim of this review was 
to present some preharvest and postharvest strategies for integrated FHB 
management in wheat production.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Science
C3  - Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“
T1  - Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_790
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Caro Petrović, Violeta and Ostojić Andrić, Dušica",
year = "2022",
abstract = "Fusarium head blight (FHB) is economically the most important wheat disease, 
causing yield and quality losses. Fungal species from the genus Fusarium are the 
main causative agents of FHB, with Fusarium graminearum as the predominant 
species. F. graminearum synthesizes more different secondary metabolites 
(mycotoxins). In wheat, the most studied mycotoxins are trichothecenes and 
zearalenone produced by F. graminearum. Trichothecene deoxynivalenol (DON) 
and its acetylated forms 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON) are the most detected in wheat grains. Strategies for 
controlling FHB and DON in wheat includе different preharvest and postharvest 
measures, emphasizing integrated approaches. The main aim of this review was 
to present some preharvest and postharvest strategies for integrated FHB 
management in wheat production.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Science",
journal = "Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“",
title = "Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_790"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Caro Petrović, V.,& Ostojić Andrić, D.. (2022). Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat. in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“
The Balkans Scientific Center of the Russian Academy of Natural Science..
https://hdl.handle.net/21.15107/rcub_ristocar_790
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Caro Petrović V, Ostojić Andrić D. Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat. in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“. 2022;.
https://hdl.handle.net/21.15107/rcub_ristocar_790 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Bijelić, Zorica, Caro Petrović, Violeta, Ostojić Andrić, Dušica, "Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat" in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“ (2022),
https://hdl.handle.net/21.15107/rcub_ristocar_790 .

Contamination of maize kernels with mycotoxins after harvest

Obradović, Ana; Stanković, Slavica; Krnjaja, Vesna; Kostadinović, Marija; Ristić, Danijela; Jauković, Marko

(2022)

TY  - CONF
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Jauković, Marko
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/795
AB  - The species of the genus Fusarium and Aspergillus are the most common pathogens of maize 
kernels worldwide. The most common species among them are F. verticillioides, F. 
graminearum and A. flavus. These fungi produce a wide spectrum of mycotoxins, among which 
the most common are fusariotoxins: trichothecenes-deoxynivalenol (DON), fumonisins (FBs) 
and aflatoxins: aflatoxin B1 (AFB1). The aim of this study was to examine the mycopopulation 
on maize kernels after harvest, as well as the concentration of their mycotoxins in kernels.
Standard mycological examinations of maize kernels revealed the presence of toxigenic species 
of fungi from three genus, Fusarium, Aspergillus and Penicillium. In the examined samples, the 
species F. verticillioides was most often isolated in majority of hybrids, with a maximum 
incidence of 32%, while the presence of Aspergillus spp. was from 0 to 17%. Mycotoxicological 
analysis of maize kernels was performed by the ELISA method using a commercial kit (Tecna 
S.r.l., Italy). All analysed samples were positive for the presence of at least one mycotoxin. The 
differences between the examined hybrids in the concentration of mycotoxins in the grain were 
statistically significant (P <0.001) for DON and FBs, but not for the content of AFB1. Likewise, 
the interaction between hybrids and localities was statistically significant (P <0.001) for DON 
and FBS content, while it was not statistically significant for AFB1 concentration. The average 
DON concentrations were 127,55 µg kg–1
, FBs 3050,21 µg kg–1
, and AFB1 2,98 µg kg–1
.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Contamination of maize kernels with mycotoxins after harvest
SP  - 195
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_795
ER  - 
@conference{
author = "Obradović, Ana and Stanković, Slavica and Krnjaja, Vesna and Kostadinović, Marija and Ristić, Danijela and Jauković, Marko",
year = "2022",
abstract = "The species of the genus Fusarium and Aspergillus are the most common pathogens of maize 
kernels worldwide. The most common species among them are F. verticillioides, F. 
graminearum and A. flavus. These fungi produce a wide spectrum of mycotoxins, among which 
the most common are fusariotoxins: trichothecenes-deoxynivalenol (DON), fumonisins (FBs) 
and aflatoxins: aflatoxin B1 (AFB1). The aim of this study was to examine the mycopopulation 
on maize kernels after harvest, as well as the concentration of their mycotoxins in kernels.
Standard mycological examinations of maize kernels revealed the presence of toxigenic species 
of fungi from three genus, Fusarium, Aspergillus and Penicillium. In the examined samples, the 
species F. verticillioides was most often isolated in majority of hybrids, with a maximum 
incidence of 32%, while the presence of Aspergillus spp. was from 0 to 17%. Mycotoxicological 
analysis of maize kernels was performed by the ELISA method using a commercial kit (Tecna 
S.r.l., Italy). All analysed samples were positive for the presence of at least one mycotoxin. The 
differences between the examined hybrids in the concentration of mycotoxins in the grain were 
statistically significant (P <0.001) for DON and FBs, but not for the content of AFB1. Likewise, 
the interaction between hybrids and localities was statistically significant (P <0.001) for DON 
and FBS content, while it was not statistically significant for AFB1 concentration. The average 
DON concentrations were 127,55 µg kg–1
, FBs 3050,21 µg kg–1
, and AFB1 2,98 µg kg–1
.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Contamination of maize kernels with mycotoxins after harvest",
pages = "195-195",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_795"
}
Obradović, A., Stanković, S., Krnjaja, V., Kostadinović, M., Ristić, D.,& Jauković, M.. (2022). Contamination of maize kernels with mycotoxins after harvest. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 195-195.
https://hdl.handle.net/21.15107/rcub_ristocar_795
Obradović A, Stanković S, Krnjaja V, Kostadinović M, Ristić D, Jauković M. Contamination of maize kernels with mycotoxins after harvest. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:195-195.
https://hdl.handle.net/21.15107/rcub_ristocar_795 .
Obradović, Ana, Stanković, Slavica, Krnjaja, Vesna, Kostadinović, Marija, Ristić, Danijela, Jauković, Marko, "Contamination of maize kernels with mycotoxins after harvest" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):195-195,
https://hdl.handle.net/21.15107/rcub_ristocar_795 .

Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Nikolić, Milica; Savić, Iva; Mandić, Violeta; Bijelić, Zorica

(2022)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/801
AB  - Fusarium infection and deoxynivalenol (DON) contamination in seven 
durum wheat lines kernel (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, 
ZP DSP 66, and one international durum line Cimmyt 7817) during two harvest seasons 
(2015–2016) has been studied. The four Fusarium species, F. graminearum, F. proliferatum, 
F. sporotrichioides, and F. verticillioides, were identified in 2015. A different structure of 
the Fusarium population, which in addition to F. graminearum, F. sporotrichioides and F. 
verticillioides, also comprised F. poae, F. semitectum, and F. subglutinans, was identified 
in 2016. F. graminearum was the predominant species in the durum wheat lines kernels and 
the potential producer of DON. The other Fusarium spp. were isolated sporadically and with 
a low incidence in the kernels. The incidence of F. graminearum and DON levels were 
significantly affected by the wheat genotypes and studied years and these parameters were 
negatively correlated. The incidence of F. graminearum was significantly higher in 2015 
(75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 2016 
(3.636 mg kg-1) compared to 2015 (1.126 mg kg-1). Statistically, there was a significantly 
higher incidence of F. graminearum in ZP DSP 66 (73.00%) and ZP 120 (72.75%) durum 
wheat lines than in the other durum genotypes. DON level was the highest in durum wheat 
line ZP 120 (3.854 mg kg-1). Considering all treatments tested, the mean DON level was 
2.381 mg kg-1, while the mean incidence of F. graminearum was 69.64%. Тested durum 
wheat lines showed susceptibility to F. graminearum, resulting in high DON levels in kernels. 
The results obtained suggest the importance of using the lines with improved resistance to 
Fusarium head blight in the breeding programs for new durum wheat cultivars.
T2  - Matica Srpska Journal for Natural Sciences
T1  - Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol
VL  - 
IS  - 143
SP  - 27
EP  - 38
DO  - 10.2298/ZMSPN2243027K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Savić, Iva and Mandić, Violeta and Bijelić, Zorica",
year = "2022",
abstract = "Fusarium infection and deoxynivalenol (DON) contamination in seven 
durum wheat lines kernel (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, 
ZP DSP 66, and one international durum line Cimmyt 7817) during two harvest seasons 
(2015–2016) has been studied. The four Fusarium species, F. graminearum, F. proliferatum, 
F. sporotrichioides, and F. verticillioides, were identified in 2015. A different structure of 
the Fusarium population, which in addition to F. graminearum, F. sporotrichioides and F. 
verticillioides, also comprised F. poae, F. semitectum, and F. subglutinans, was identified 
in 2016. F. graminearum was the predominant species in the durum wheat lines kernels and 
the potential producer of DON. The other Fusarium spp. were isolated sporadically and with 
a low incidence in the kernels. The incidence of F. graminearum and DON levels were 
significantly affected by the wheat genotypes and studied years and these parameters were 
negatively correlated. The incidence of F. graminearum was significantly higher in 2015 
(75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 2016 
(3.636 mg kg-1) compared to 2015 (1.126 mg kg-1). Statistically, there was a significantly 
higher incidence of F. graminearum in ZP DSP 66 (73.00%) and ZP 120 (72.75%) durum 
wheat lines than in the other durum genotypes. DON level was the highest in durum wheat 
line ZP 120 (3.854 mg kg-1). Considering all treatments tested, the mean DON level was 
2.381 mg kg-1, while the mean incidence of F. graminearum was 69.64%. Тested durum 
wheat lines showed susceptibility to F. graminearum, resulting in high DON levels in kernels. 
The results obtained suggest the importance of using the lines with improved resistance to 
Fusarium head blight in the breeding programs for new durum wheat cultivars.",
journal = "Matica Srpska Journal for Natural Sciences",
title = "Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol",
volume = "",
number = "143",
pages = "27-38",
doi = "10.2298/ZMSPN2243027K"
}
Krnjaja, V., Stanković, S., Obradović, A., Nikolić, M., Savić, I., Mandić, V.,& Bijelić, Z.. (2022). Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol. in Matica Srpska Journal for Natural Sciences, (143), 27-38.
https://doi.org/10.2298/ZMSPN2243027K
Krnjaja V, Stanković S, Obradović A, Nikolić M, Savić I, Mandić V, Bijelić Z. Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol. in Matica Srpska Journal for Natural Sciences. 2022;(143):27-38.
doi:10.2298/ZMSPN2243027K .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Savić, Iva, Mandić, Violeta, Bijelić, Zorica, "Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol" in Matica Srpska Journal for Natural Sciences, , no. 143 (2022):27-38,
https://doi.org/10.2298/ZMSPN2243027K . .

Toxicological profile pathogenic species on maize in Serbia

Obradović, Ana; Nikolić, Milica; Savić, Iva; Krnjaja, Vesna; Stanković, Slavica

(2022)

TY  - CONF
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/803
AB  - Fusarium and Aspergillus toxigenic species are considered to be the most common pathogens 
in maize. Agroecological conditions in Serbia favour the development of numerous pathogenic 
and toxigenic species of the genus Fusarium and Aspergillus. Global climate change may 
favour the contamination of maize with high levels of aflatoxins and fusariotoxins in temperate 
areas, such as Serbia. Fusarium graminearum synthesises mycotoxins from which the most 
important are type B trichothecene - deoxynivalenol (DON), its acetyl-ester derivatives (3-
АDON and 15-АDON) and nivalenol (NIV). The two most important species of fungi 
responsible for aspergillosis kernel and ear rot are Aspergillus flavus and Aspergillus 
parasiticus, producers of aflatoxins (B1, B2, G1, and G2). 
Isolates of F. graminearum and A. parasiticus identified from maize grain samples collected 
over 12 years from 40 localities in Serbia were analyzed. Production potential of isolates were 
determined by high-performance liquid chromatography method (HPLC).
Results of perennial research showed high variability in the concentrations of mycotoxins 
deoxynivalenol and aflatoxins in maize kernels. Detected concentrations of DON varied from 
10,9 to 154.6 µg/g (average 47,2 µg/g), while 3ADON concentrations ranged from 3.42 to 71.6 
µg/g (average 15,6 µg/g ) and 15ADON from 5.4 to 121.3 µg/g (average 29,8 µg/g). Mycotoxin 
15ADON were predominant in observed samples. The average synthesis of aflatoxin B1 was 
4145.97 µg/kg (range 14.24 to 7361.03 µg/kg) by which the isolates of this group were 
classified into strong producers of AFB1. The average synthesis of aflatoxin B2 was 403.55 
µg/kg, aflatoxin G1 3490.07 µg/kg and G2 198.29 µg/kg averaging, respectivel
C3  - Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia
T1  - Toxicological profile pathogenic species on maize in Serbia
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_803
ER  - 
@conference{
author = "Obradović, Ana and Nikolić, Milica and Savić, Iva and Krnjaja, Vesna and Stanković, Slavica",
year = "2022",
abstract = "Fusarium and Aspergillus toxigenic species are considered to be the most common pathogens 
in maize. Agroecological conditions in Serbia favour the development of numerous pathogenic 
and toxigenic species of the genus Fusarium and Aspergillus. Global climate change may 
favour the contamination of maize with high levels of aflatoxins and fusariotoxins in temperate 
areas, such as Serbia. Fusarium graminearum synthesises mycotoxins from which the most 
important are type B trichothecene - deoxynivalenol (DON), its acetyl-ester derivatives (3-
АDON and 15-АDON) and nivalenol (NIV). The two most important species of fungi 
responsible for aspergillosis kernel and ear rot are Aspergillus flavus and Aspergillus 
parasiticus, producers of aflatoxins (B1, B2, G1, and G2). 
Isolates of F. graminearum and A. parasiticus identified from maize grain samples collected 
over 12 years from 40 localities in Serbia were analyzed. Production potential of isolates were 
determined by high-performance liquid chromatography method (HPLC).
Results of perennial research showed high variability in the concentrations of mycotoxins 
deoxynivalenol and aflatoxins in maize kernels. Detected concentrations of DON varied from 
10,9 to 154.6 µg/g (average 47,2 µg/g), while 3ADON concentrations ranged from 3.42 to 71.6 
µg/g (average 15,6 µg/g ) and 15ADON from 5.4 to 121.3 µg/g (average 29,8 µg/g). Mycotoxin 
15ADON were predominant in observed samples. The average synthesis of aflatoxin B1 was 
4145.97 µg/kg (range 14.24 to 7361.03 µg/kg) by which the isolates of this group were 
classified into strong producers of AFB1. The average synthesis of aflatoxin B2 was 403.55 
µg/kg, aflatoxin G1 3490.07 µg/kg and G2 198.29 µg/kg averaging, respectivel",
journal = "Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia",
title = "Toxicological profile pathogenic species on maize in Serbia",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_803"
}
Obradović, A., Nikolić, M., Savić, I., Krnjaja, V.,& Stanković, S.. (2022). Toxicological profile pathogenic species on maize in Serbia. in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia, 58-58.
https://hdl.handle.net/21.15107/rcub_ristocar_803
Obradović A, Nikolić M, Savić I, Krnjaja V, Stanković S. Toxicological profile pathogenic species on maize in Serbia. in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia. 2022;:58-58.
https://hdl.handle.net/21.15107/rcub_ristocar_803 .
Obradović, Ana, Nikolić, Milica, Savić, Iva, Krnjaja, Vesna, Stanković, Slavica, "Toxicological profile pathogenic species on maize in Serbia" in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia (2022):58-58,
https://hdl.handle.net/21.15107/rcub_ristocar_803 .

Fuzariotoksini na zrnu pšenice

Obradović, Ana; Stanković, Slavica; Krnjaja, Vesna; Nikolić, Milica; Savić, Iva; Stevanović, Milan; Stanković, Goran

(2022)

TY  - CONF
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/802
AB  - Pšenica je na prvom mestu po površinama na kojima se gaji u svetu i značaju u 
prehrambenoj industriji. Različiti načini upotrebe zrna i suve mase žita u ishrani ljudi i 
životinja, kao i u industrijskoj preradi, ukazuju na to da ona imaju veliku upotrebnu vrednost. 
Zrno pšenice u različitim agroekološkim uslovima može biti zaraženo brojnim vrstama gljiva, 
kako u polju, tako i tokom čuvanja u skladištima. Vrste roda Fusarium su najčešći patogeni 
zrna pšenice, koji su posebno značajni, jer imaju sposobnost da sintetišu veliki spektar 
mikotoksina od kojih su najznačajniji fumonizin B1 (FB1), deoksinivalenol (DON) i zearalenon 
(ZEA). Cilj ovog rada bio je da se ispita koncentracija pomenutih mikotoksina u zrnu pšenice 
nakon žetve. 
Analizirano je 20 uzoraka zrna pšenice prikupljene iz različitih agroekoloških uslova u 
Srbiji. Pet grama od svakog osušenog i samlevenog uzorka je ekstrahovano i filtrirano 
rastvorom metanola i vode. Koncentracija mikotoksina je određena pomoću ELISA testa 
prema upustvu proizvođača (Tecna S.r.l., Italy, Celer Fumo Test Kit, Celer DON Gold Test Kit i 
Celer Zon Test Kit). U svim analiziranim uzorcima utvrđeno je prisustvo bar jednog 
ispitivanog mikotoksina. Ustanovljena koncentracija DON iznosila je od 0,596 do 2,258 µg/g 
(prosek 1,152 µg/g), FB1 od 0,852 do 6,253 µg/g (prosek 2,021 µg/кg) dok je mikotoksin ZEA 
bio prisutan u intervalu od 8,42 do 58,25 µg/кg (prosek 22,98 µg/кg). Utvrđene koncentracije 
ZEA i DON su bile niže od maksimalno dozvoljenih koncentracija koje su propisane 
Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani 
i hrani za životinje (Službeni glasnik Republike Srbije, 2019), dok su u pet uzoraka FB1
detektovani u koncentraciji većoj od dozvoljenih u ljudskoj hrani.
C3  - Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine
T1  - Fuzariotoksini na zrnu pšenice
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_802
ER  - 
@conference{
author = "Obradović, Ana and Stanković, Slavica and Krnjaja, Vesna and Nikolić, Milica and Savić, Iva and Stevanović, Milan and Stanković, Goran",
year = "2022",
abstract = "Pšenica je na prvom mestu po površinama na kojima se gaji u svetu i značaju u 
prehrambenoj industriji. Različiti načini upotrebe zrna i suve mase žita u ishrani ljudi i 
životinja, kao i u industrijskoj preradi, ukazuju na to da ona imaju veliku upotrebnu vrednost. 
Zrno pšenice u različitim agroekološkim uslovima može biti zaraženo brojnim vrstama gljiva, 
kako u polju, tako i tokom čuvanja u skladištima. Vrste roda Fusarium su najčešći patogeni 
zrna pšenice, koji su posebno značajni, jer imaju sposobnost da sintetišu veliki spektar 
mikotoksina od kojih su najznačajniji fumonizin B1 (FB1), deoksinivalenol (DON) i zearalenon 
(ZEA). Cilj ovog rada bio je da se ispita koncentracija pomenutih mikotoksina u zrnu pšenice 
nakon žetve. 
Analizirano je 20 uzoraka zrna pšenice prikupljene iz različitih agroekoloških uslova u 
Srbiji. Pet grama od svakog osušenog i samlevenog uzorka je ekstrahovano i filtrirano 
rastvorom metanola i vode. Koncentracija mikotoksina je određena pomoću ELISA testa 
prema upustvu proizvođača (Tecna S.r.l., Italy, Celer Fumo Test Kit, Celer DON Gold Test Kit i 
Celer Zon Test Kit). U svim analiziranim uzorcima utvrđeno je prisustvo bar jednog 
ispitivanog mikotoksina. Ustanovljena koncentracija DON iznosila je od 0,596 do 2,258 µg/g 
(prosek 1,152 µg/g), FB1 od 0,852 do 6,253 µg/g (prosek 2,021 µg/кg) dok je mikotoksin ZEA 
bio prisutan u intervalu od 8,42 do 58,25 µg/кg (prosek 22,98 µg/кg). Utvrđene koncentracije 
ZEA i DON su bile niže od maksimalno dozvoljenih koncentracija koje su propisane 
Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani 
i hrani za životinje (Službeni glasnik Republike Srbije, 2019), dok su u pet uzoraka FB1
detektovani u koncentraciji većoj od dozvoljenih u ljudskoj hrani.",
journal = "Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine",
title = "Fuzariotoksini na zrnu pšenice",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_802"
}
Obradović, A., Stanković, S., Krnjaja, V., Nikolić, M., Savić, I., Stevanović, M.,& Stanković, G.. (2022). Fuzariotoksini na zrnu pšenice. in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine, 48-48.
https://hdl.handle.net/21.15107/rcub_ristocar_802
Obradović A, Stanković S, Krnjaja V, Nikolić M, Savić I, Stevanović M, Stanković G. Fuzariotoksini na zrnu pšenice. in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine. 2022;:48-48.
https://hdl.handle.net/21.15107/rcub_ristocar_802 .
Obradović, Ana, Stanković, Slavica, Krnjaja, Vesna, Nikolić, Milica, Savić, Iva, Stevanović, Milan, Stanković, Goran, "Fuzariotoksini na zrnu pšenice" in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine (2022):48-48,
https://hdl.handle.net/21.15107/rcub_ristocar_802 .

Novel toxigenic species on maize kernels in Southeastern Europe

Stanković, Slavica; Obradović, Ana; Nikolić, Milica; Nikolić, Ana; Krnjaja, Vesna

(2022)

TY  - CONF
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Krnjaja, Vesna
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/794
AB  - In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Novel toxigenic species on maize kernels in Southeastern Europe
SP  - 194
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_794
ER  - 
@conference{
author = "Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Nikolić, Ana and Krnjaja, Vesna",
year = "2022",
abstract = "In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Novel toxigenic species on maize kernels in Southeastern Europe",
pages = "194-194",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_794"
}
Stanković, S., Obradović, A., Nikolić, M., Nikolić, A.,& Krnjaja, V.. (2022). Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794
Stanković S, Obradović A, Nikolić M, Nikolić A, Krnjaja V. Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794 .
Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Nikolić, Ana, Krnjaja, Vesna, "Novel toxigenic species on maize kernels in Southeastern Europe" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):194-194,
https://hdl.handle.net/21.15107/rcub_ristocar_794 .

Fusarium and deoxynivalenol contamination of durum wheat lines kernels

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Nikolić, Milica; Savić, Iva; Mandić, Violeta; Bijelić, Zorica

(2022)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/793
AB  - Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.
C3  - Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia
T1  - Fusarium and deoxynivalenol contamination of durum wheat lines kernels
SP  - 17
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_793
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Savić, Iva and Mandić, Violeta and Bijelić, Zorica",
year = "2022",
abstract = "Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.",
journal = "Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia",
title = "Fusarium and deoxynivalenol contamination of durum wheat lines kernels",
pages = "17-17",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_793"
}
Krnjaja, V., Stanković, S., Obradović, A., Nikolić, M., Savić, I., Mandić, V.,& Bijelić, Z.. (2022). Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia, 17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793
Krnjaja V, Stanković S, Obradović A, Nikolić M, Savić I, Mandić V, Bijelić Z. Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia. 2022;:17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Savić, Iva, Mandić, Violeta, Bijelić, Zorica, "Fusarium and deoxynivalenol contamination of durum wheat lines kernels" in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia (2022):17-17,
https://hdl.handle.net/21.15107/rcub_ristocar_793 .

First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia

Obradović, Ana; Stepanović, Jelena; Krnjaja, Vesna; Bulajić, Aleksandra; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(The American Phytopathological Society, 2022)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/785
AB  - The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (genealogical concordance phylogenetic species recognition) revealed the existence of 15 phylogenetic species within what is now recognized as the Fusarium graminearum species complex (FGSC) (Sarver et al. 2011). During 1996–2008, a collection of FGSC isolates was established at the Maize Research Institute, Zemun Polje, and isolates originating from wheat (5), maize (3), and barley (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5 to 53.1 × 4.6 to 5.4 µm, n = 50) of all 10 isolates on potato dextrose agar (PDA) were consistent with descriptions of F. graminearum (Leslie and Summerell 2006; O’Donnell et al. 2004). Total DNA was isolated from mycelium removed from 7-day-old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3, and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b, and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in GenBank under accession numbers MF974399–MF974408 (TEF-1α), MG063783–MG063792 (β-tubulin), and MF999139–MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006) shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728), and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99 to 100% nucleotide similarity with F. graminearum NRRL 28439 (O’Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterházy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day-old colonies on PDA, and 30 ml of a conidial suspension (1 × 105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1 to 7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4–5.1 for F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON, and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Tóth et al. 2005) and in Korea on barley, corn, and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness, and toxicological profile of F. vorosii.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia
VL  - 106
IS  - 2
SP  - 758
EP  - 758
DO  - https://doi.org/10.1094/PDIS-04-21-0715-PDN
ER  - 
@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2022",
abstract = "The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (genealogical concordance phylogenetic species recognition) revealed the existence of 15 phylogenetic species within what is now recognized as the Fusarium graminearum species complex (FGSC) (Sarver et al. 2011). During 1996–2008, a collection of FGSC isolates was established at the Maize Research Institute, Zemun Polje, and isolates originating from wheat (5), maize (3), and barley (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5 to 53.1 × 4.6 to 5.4 µm, n = 50) of all 10 isolates on potato dextrose agar (PDA) were consistent with descriptions of F. graminearum (Leslie and Summerell 2006; O’Donnell et al. 2004). Total DNA was isolated from mycelium removed from 7-day-old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3, and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b, and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in GenBank under accession numbers MF974399–MF974408 (TEF-1α), MG063783–MG063792 (β-tubulin), and MF999139–MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006) shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728), and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99 to 100% nucleotide similarity with F. graminearum NRRL 28439 (O’Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterházy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day-old colonies on PDA, and 30 ml of a conidial suspension (1 × 105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1 to 7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4–5.1 for F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON, and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Tóth et al. 2005) and in Korea on barley, corn, and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness, and toxicological profile of F. vorosii.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia",
volume = "106",
number = "2",
pages = "758-758",
doi = "https://doi.org/10.1094/PDIS-04-21-0715-PDN"
}
Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2022). First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease
The American Phytopathological Society., 106(2), 758-758.
https://doi.org/https://doi.org/10.1094/PDIS-04-21-0715-PDN
Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease. 2022;106(2):758-758.
doi:https://doi.org/10.1094/PDIS-04-21-0715-PDN .
Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia" in Plant Disease, 106, no. 2 (2022):758-758,
https://doi.org/https://doi.org/10.1094/PDIS-04-21-0715-PDN . .

First report of head blight of wheat caused by Fusarium vorosii in Serbia

Obradović, Ana; Stepanović, Jelena; Krnjaja, Vesna; Bulajić, Aleksandra; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(The American Phytopathological Society, USA, 2021-07)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021-07
UR  - http://r.istocar.bg.ac.rs/handle/123456789/743
AB  - The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (Genealogical Concordance Phylogenetic Species Recognition) revealed the existence of 15 phylogenetic species within what is now recognised as the Fusarium graminearum Species Complex (FGSC) (Sarver et al. 2011). During 1996-2008, a MRIZP collection of FGSC isolates was established and isolates originating from wheat (5), maize (3) and barely (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5-53.1 × 4.6-5.4 µm, No 50) of all 10 isolates on PDA were consistent with descriptions of F. graminearum (O'Donnell et al. 2004, Leslie and Summerell 2006). Total DNA was isolated from mycelium removed from 7-day old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3 and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in NCBI under accession numbers MF974399 - MF974408 (TEF-1α), MG063783 - MG063792 (β-tubulin) and MF999139 - MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006), shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728) and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99% to 100% nucleotide similarity with F. graminearum NRRL 28439 (O'Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterhazy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day colonies on PDA, and 30 ml of a conidia suspension (1x105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1-7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4 -5.1 of F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Toth et al. 2005) and Korea on barley, corn and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness and toxicological profile of F. vorosii.
PB  - The American Phytopathological Society, USA
T2  - Plant Disease
T1  - First report of head blight of wheat caused by Fusarium vorosii in Serbia
DO  - 10.1094/PDIS-04-21-0715-PDN
ER  - 
@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2021-07",
abstract = "The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (Genealogical Concordance Phylogenetic Species Recognition) revealed the existence of 15 phylogenetic species within what is now recognised as the Fusarium graminearum Species Complex (FGSC) (Sarver et al. 2011). During 1996-2008, a MRIZP collection of FGSC isolates was established and isolates originating from wheat (5), maize (3) and barely (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5-53.1 × 4.6-5.4 µm, No 50) of all 10 isolates on PDA were consistent with descriptions of F. graminearum (O'Donnell et al. 2004, Leslie and Summerell 2006). Total DNA was isolated from mycelium removed from 7-day old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3 and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in NCBI under accession numbers MF974399 - MF974408 (TEF-1α), MG063783 - MG063792 (β-tubulin) and MF999139 - MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006), shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728) and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99% to 100% nucleotide similarity with F. graminearum NRRL 28439 (O'Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterhazy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day colonies on PDA, and 30 ml of a conidia suspension (1x105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1-7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4 -5.1 of F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Toth et al. 2005) and Korea on barley, corn and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness and toxicological profile of F. vorosii.",
publisher = "The American Phytopathological Society, USA",
journal = "Plant Disease",
title = "First report of head blight of wheat caused by Fusarium vorosii in Serbia",
doi = "10.1094/PDIS-04-21-0715-PDN"
}
Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2021-07). First report of head blight of wheat caused by Fusarium vorosii in Serbia. in Plant Disease
The American Phytopathological Society, USA..
https://doi.org/10.1094/PDIS-04-21-0715-PDN
Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First report of head blight of wheat caused by Fusarium vorosii in Serbia. in Plant Disease. 2021;.
doi:10.1094/PDIS-04-21-0715-PDN .
Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of head blight of wheat caused by Fusarium vorosii in Serbia" in Plant Disease (2021-07),
https://doi.org/10.1094/PDIS-04-21-0715-PDN . .
3

Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja; Radović, Čedomir

(2021-02-25)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Radović, Čedomir
PY  - 2021-02-25
UR  - http://r.istocar.bg.ac.rs/handle/123456789/730
AB  - The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.
T2  - Crop Protection
T1  - Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels
VL  - 144
SP  - 105601
DO  - https://doi.org/10.1016/j.cropro.2021.105601
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Radović, Čedomir",
year = "2021-02-25",
abstract = "The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.",
journal = "Crop Protection",
title = "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels",
volume = "144",
pages = "105601",
doi = "https://doi.org/10.1016/j.cropro.2021.105601"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Radović, Č.. (2021-02-25). Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection, 144, 105601.
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Vasić T, Radović Č. Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection. 2021;144:105601.
doi:https://doi.org/10.1016/j.cropro.2021.105601 .
Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Radović, Čedomir, "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels" in Crop Protection, 144 (2021-02-25):105601,
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601 . .

The effect of climate conditions on aflatoxin contamination of cereal grains and feeds

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Mandić, Violeta; Bijelić, Zorica; Jauković, Marko

(Institute for Animal Husbandry, Belgrade-Zemun, 2019)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Jauković, Marko
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/626
AB  - Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
T1  - The effect of climate conditions on aflatoxin contamination of cereal grains and feeds
SP  - 580
EP  - 591
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_626
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Jauković, Marko",
year = "2019",
abstract = "Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia",
title = "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds",
pages = "580-591",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_626"
}
Krnjaja, V., Stanković, S., Obradović, A., Petrović, T., Mandić, V., Bijelić, Z.,& Jauković, M.. (2019). The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun., 580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626
Krnjaja V, Stanković S, Obradović A, Petrović T, Mandić V, Bijelić Z, Jauković M. The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia. 2019;:580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Jauković, Marko, "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds" in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia (2019):580-591,
https://hdl.handle.net/21.15107/rcub_ristocar_626 .

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica Ž.; Krnjaja, Vesna; Nikolic, Ana; Ignjatović-Micić, Dragana; Stepanović, Jelena; Duduk, Bojan

(Serbian Genetics Society, Belgrade, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica Ž.
AU  - Krnjaja, Vesna
AU  - Nikolic, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/526
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Serbian Genetics Society, Belgrade
T2  - Genetika-Belgrade
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica Ž. and Krnjaja, Vesna and Nikolic, Ana and Ignjatović-Micić, Dragana and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Serbian Genetics Society, Belgrade",
journal = "Genetika-Belgrade",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S. Ž., Krnjaja, V., Nikolic, A., Ignjatović-Micić, D., Stepanović, J.,& Duduk, B.. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade
Serbian Genetics Society, Belgrade., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković SŽ, Krnjaja V, Nikolic A, Ignjatović-Micić D, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade. 2017;49(1):355-364.
doi:10.2298/GENSR1701355O .
Obradović, Ana, Stanković, Slavica Ž., Krnjaja, Vesna, Nikolic, Ana, Ignjatović-Micić, Dragana, Stepanović, Jelena, Duduk, Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" in Genetika-Belgrade, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O . .
7
3
6

Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia

Obradović, Ana; Stanković, Slavica Ž.; Stevanović, Milan; Krnjaja, Vesna; Bulajić, Aleksandra; Delibašić, Goran

(Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica Ž.
AU  - Stevanović, Milan
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Delibašić, Goran
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/546
AB  - Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum.
AB  - Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia
T1  - Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji
VL  - 45
IS  - 3
SP  - 277
EP  - 286
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4584
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica Ž. and Stevanović, Milan and Krnjaja, Vesna and Bulajić, Aleksandra and Delibašić, Goran",
year = "2017",
abstract = "Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum., Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia, Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji",
volume = "45",
number = "3",
pages = "277-286",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4584"
}
Obradović, A., Stanković, S. Ž., Stevanović, M., Krnjaja, V., Bulajić, A.,& Delibašić, G.. (2017). Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(3), 277-286.
https://hdl.handle.net/21.15107/rcub_agrospace_4584
Obradović A, Stanković SŽ, Stevanović M, Krnjaja V, Bulajić A, Delibašić G. Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia. in Biljni lekar. 2017;45(3):277-286.
https://hdl.handle.net/21.15107/rcub_agrospace_4584 .
Obradović, Ana, Stanković, Slavica Ž., Stevanović, Milan, Krnjaja, Vesna, Bulajić, Aleksandra, Delibašić, Goran, "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia" in Biljni lekar, 45, no. 3 (2017):277-286,
https://hdl.handle.net/21.15107/rcub_agrospace_4584 .

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica Ž.; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko M.

(Institute for Animal Husbandry, Belgrade, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica Ž.
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko M.
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/503
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica Ž. and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko M.",
year = "2016",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S. Ž., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. M.. (2016). Fungal and mycotoxin contamination of maize hybrids in different maturity groups. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković SŽ, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković MM. Fungal and mycotoxin contamination of maize hybrids in different maturity groups. in Biotechnology in Animal Husbandry. 2016;32(1):71-81.
doi:10.2298/BAH1601071K .
Krnjaja, Vesna, Stanković, Slavica Ž., Obradović, Ana, Mandić, Violeta, Bijelić, Zorica, Vasić, Tanja, Jauković, Marko M., "Fungal and mycotoxin contamination of maize hybrids in different maturity groups" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K . .
4

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica Ž.; Petrović, Tanja S.; Vasić, Tanja; Obradović, Ana

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Vasić, Tanja
AU  - Obradović, Ana
PY  - 2015
UR  - http://r.istocar.bg.ac.rs/handle/123456789/453
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  (lt) = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  (lt) = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON. (C) 2014 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Vasić, Tanja and Obradović, Ana",
year = "2015",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  (lt) = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  (lt) = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON. (C) 2014 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Vasić, T.,& Obradović, A.. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković SŽ, Petrović TS, Vasić T, Obradović A. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection. 2015;67:251-256.
doi:10.1016/j.cropro.2014.11.001 .
Krnjaja, Vesna, Mandić, Violeta, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Vasić, Tanja, Obradović, Ana, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" in Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 . .
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