TY  - JOUR
AU  - Maletić, Milan
AU  - Aleksić, Nevenka
AU  - Vejnović, Branislav
AU  - Nikšić, Dragan
AU  - Kulić, Milan
AU  - Djukić, Božidar
AU  - Ćirković, Dragan
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/483
AB  - The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.
PB  - Croatian Dairy Union, Zagreb
T2  - Mljekarstvo
T1  - Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia
VL  - 66
IS  - 3
SP  - 198
EP  - 205
DO  - 10.15567/mljekarstvo.2016.0304
ER  - 

@article{
author = "Maletić, Milan and Aleksić, Nevenka and Vejnović, Branislav and Nikšić, Dragan and Kulić, Milan and Djukić, Božidar and Ćirković, Dragan",
year = "2016",
abstract = "The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.",
publisher = "Croatian Dairy Union, Zagreb",
journal = "Mljekarstvo",
title = "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia",
volume = "66",
number = "3",
pages = "198-205",
doi = "10.15567/mljekarstvo.2016.0304"
}

Maletić, M., Aleksić, N., Vejnović, B., Nikšić, D., Kulić, M., Djukić, B.,& Ćirković, D.. (2016). Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo
Croatian Dairy Union, Zagreb., 66(3), 198-205.
https://doi.org/10.15567/mljekarstvo.2016.0304

Maletić M, Aleksić N, Vejnović B, Nikšić D, Kulić M, Djukić B, Ćirković D. Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo. 2016;66(3):198-205.
doi:10.15567/mljekarstvo.2016.0304 .

Maletić, Milan, Aleksić, Nevenka, Vejnović, Branislav, Nikšić, Dragan, Kulić, Milan, Djukić, Božidar, Ćirković, Dragan, "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia" in Mljekarstvo, 66, no. 3 (2016):198-205,
https://doi.org/10.15567/mljekarstvo.2016.0304 . .