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Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia

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2016
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Authors
Maletić, Milan
Aleksić, Nevenka
Vejnović, Branislav
Nikšić, Dragan
Kulić, Milan
Djukić, Božidar
Ćirković, Dragan
Article (Published version)
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Abstract
The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype w...as not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.

Keywords:
kappa-casein / beta-lactoglobulin / polymorphism / PCR-RFLP / Holstein-Friesian / Busha cattle
Source:
Mljekarstvo, 2016, 66, 3, 198-205
Publisher:
  • Croatian Dairy Union, Zagreb
Projects:
  • Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)

DOI: 10.15567/mljekarstvo.2016.0304

ISSN: 0026-704X

WoS: 000379483400004

Scopus: 2-s2.0-84979084593
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URI
http://r.istocar.bg.ac.rs/handle/123456789/483
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