dc.description.abstract | Pirot breed is a highly endangered autochthonous population of
sheep in Serbia, from which several important national brands originate: Pirot
lamb, Pirot carpet and Pirot hard cheese. In the available literature, there are no
data on hematological and biochemical parameters of the blood of this population.
The aim of this paper, which represents the beginning of the research, is to
determine the above indicators in the blood of Pirot Pramenka, which will
contribute to the preservation of this population. Since the breed affects the value
of blood parameters, the obtained results will be used to define reference intervals
for this population and compare with others. The study included 30 clinically
healthy sheep, 2-3 years old, from the area of Stara Planina. Blood samples were
taken by puncture to c. jugularis from which, after coagulation and centrifugation,
blood serum was extracted. Haematological analyzes were performed in the next
24 hours on a Siemens Advia 120 analyzer. The following parameters were
determined: total number of leukocytes, erythrocytes, platelets, hemoglobin
content, hematocrit value, MCV, MCH, PLT and MPV. Biochemical blood tests
were performed using Olympus AU 400 analyzers.The following parameters were
determined: total protein, albumin, globulin, urea, creatinine, glucose, total
bilirubin, cholesterol. Also, the following minerals were determined - calcium,
phosphate and magnesium, and following enzymes: AST, GGT and CK.
Descriptive statistical analysis was performed using the statistical package
STATISTICA (Version 8). Hematological indicators of Pirot pramenka are in
reference intervals for sheep, which indicates good animal health status.
Biochemical parameters of Pirot pramenka blood were within defined
physiological limits, except for creatinine and phosphorus levels, which were
below the lower limit, which is a consequence of dehydration and reduced
alimentary intake. In accordance with the obtained results, it is necessary to correct
the diet. | sr |