Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
Autori
Cekić, Bogdan
Kanzova, Hristiyana
Petrov, Georgi
Maksimović, Nevena

Ćosić, Ivan

Milovanović, Aleksandar
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm.
Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.
Ključne reči:
ram / sperm / cryopreservation / biochemical parameters / vitality / ovan / sperma / krioprezervacija / biohemijski parametri / vitalnostIzvor:
Biotechnology in animal husbandry, 2022, 38, 1, 31-39Izdavač:
- Institute for Animal Husbandry, Belgrade-Zemun, Serbia