Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
Authors
Cekić, Bogdan
Kanzova, Hristiyana
Petrov, Georgi
Maksimović, Nevena

Ćosić, Ivan

Milovanović, Aleksandar
Article (Published version)
Metadata
Show full item recordAbstract
The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm.
Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.
Keywords:
ram / sperm / cryopreservation / biochemical parameters / vitality / ovan / sperma / krioprezervacija / biohemijski parametri / vitalnostSource:
Biotechnology in animal husbandry, 2022, 38, 1, 31-39Publisher:
- Institute for Animal Husbandry, Belgrade-Zemun, Serbia
Funding / projects:
Collections
Institution/Community
RIStocarTY - JOUR AU - Cekić, Bogdan AU - Kanzova, Hristiyana AU - Petrov, Georgi AU - Maksimović, Nevena AU - Ćosić, Ivan AU - Milovanović, Aleksandar PY - 2022 UR - http://r.istocar.bg.ac.rs/handle/123456789/797 AB - The current study aimed to examine the percentage changes of viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For this purpose, five rams were examined, and two ejaculates were obtained from each ram. Ejaculates are collected by the method of artificial vagina, during the insemination campaign. All ejaculates were diluted with a 6AG extender and frozen by the Cassou’s sequin method. Sperm viability was determined by eosin and nigrosine smears, and enzyme activity was examined spectrophotometrically. As a result, the percentage of vital sperm after cryopreservation decreased by 15% (P ≤ 0.001). The freezing and thawing process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed enzymes, in relation to sperm vitality, could be used as indicators to optimize the protocols for cryopreservation of ram’s sperm. AB - Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu, ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤ 0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i koraka u procesima zamrzavanja i odmrzavanja sperme ovnova. PB - Institute for Animal Husbandry, Belgrade-Zemun, Serbia T2 - Biotechnology in animal husbandry T1 - Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams VL - 38 IS - 1 SP - 31 EP - 39 DO - 10.2298/BAH2201031C ER -
@article{ author = "Cekić, Bogdan and Kanzova, Hristiyana and Petrov, Georgi and Maksimović, Nevena and Ćosić, Ivan and Milovanović, Aleksandar", year = "2022", abstract = "The current study aimed to examine the percentage changes of viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For this purpose, five rams were examined, and two ejaculates were obtained from each ram. Ejaculates are collected by the method of artificial vagina, during the insemination campaign. All ejaculates were diluted with a 6AG extender and frozen by the Cassou’s sequin method. Sperm viability was determined by eosin and nigrosine smears, and enzyme activity was examined spectrophotometrically. As a result, the percentage of vital sperm after cryopreservation decreased by 15% (P ≤ 0.001). The freezing and thawing process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed enzymes, in relation to sperm vitality, could be used as indicators to optimize the protocols for cryopreservation of ram’s sperm., Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu, ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤ 0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.", publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia", journal = "Biotechnology in animal husbandry", title = "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams", volume = "38", number = "1", pages = "31-39", doi = "10.2298/BAH2201031C" }
Cekić, B., Kanzova, H., Petrov, G., Maksimović, N., Ćosić, I.,& Milovanović, A.. (2022). Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 38(1), 31-39. https://doi.org/10.2298/BAH2201031C
Cekić B, Kanzova H, Petrov G, Maksimović N, Ćosić I, Milovanović A. Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry. 2022;38(1):31-39. doi:10.2298/BAH2201031C .
Cekić, Bogdan, Kanzova, Hristiyana, Petrov, Georgi, Maksimović, Nevena, Ćosić, Ivan, Milovanović, Aleksandar, "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams" in Biotechnology in animal husbandry, 38, no. 1 (2022):31-39, https://doi.org/10.2298/BAH2201031C . .