Novel toxigenic species on maize kernels in Southeastern Europe
Конференцијски прилог (Објављена верзија)
Метаподаци
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In recent years, global climate changes have caused the variability of agro-climatic conditions,
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains
during the growing season and could result in economic losses in the production, as well as in
increased risk to human and animal health. These reasons and the fact that new toxigenic species
have been identified in Serbia and its neighbouring countries in a few past years, indicate the
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were
caused by extremely stressful agrometeorological conditions, high temperatures and drought
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from di...fferent samples of cereal kernels was done by using PCR-RFLP analysis
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the
presence of this species in the samples subjected to analysis. Characterization of Fg comlex
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome
fragments were sequenced and analized. Sequences were compared to the data from GeneBank.
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while
only two of them were identified as Fusarium boothii and Fusarium vorosii.