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Fusarium and deoxynivalenol contamination of durum wheat lines kernels

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2022
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Authors
Krnjaja, Vesna
Stanković, Slavica
Obradović, Ana
Nikolić, Milica
Savić, Iva
Mandić, Violeta
Bijelić, Zorica
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Abstract
Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and one international durum line Cimmyt 7817) during harvest in two growing seasons (2015- 2016) have estimated. Mycological methods were performed to determine the incidence of Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to quantify the total level of DON. Analysis of data was done by statistical method ANOVA (analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. Correlation analyses were performed by Pearson’s test. Based on morphological characteristics, four Fusarium species, F. graminearum, F. proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different structure of the Fusarium population, which in addition to F. graminearum, F. sporotrichioides and F. verticillioides, also consisted of... F. poae, F. semitectum, and F. subglutinans, was identified in 2016. F. graminearum was the predominant species and the most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and Penicillium have also been isolated. The incidence of F. graminearum and level of DON were significantly affected by the wheat genotypes and investigated years. However, obtained results for these parameters were negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum was 69.64%. Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum and as a consequence higher accumulation of mycotoxin DON. These results indicate the importance of using less susceptible or tolerant lines to the pathogens of FHB and DON accumulation in the selection programs of new durum wheat varieties.

Keywords:
Fusarium spp. / deoxynivalenol / durum wheat lines
Source:
Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022, Matica Srpska, Novi Sad, Serbia, 2022, 17-17

ISBN: 978-86-7946-387-6

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_ristocar_793
URI
http://r.istocar.bg.ac.rs/handle/123456789/793
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
RIStocar
TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/793
AB  - Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.
C3  - Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia
T1  - Fusarium and deoxynivalenol contamination of durum wheat lines kernels
SP  - 17
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_793
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Savić, Iva and Mandić, Violeta and Bijelić, Zorica",
year = "2022",
abstract = "Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.",
journal = "Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia",
title = "Fusarium and deoxynivalenol contamination of durum wheat lines kernels",
pages = "17-17",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_793"
}
Krnjaja, V., Stanković, S., Obradović, A., Nikolić, M., Savić, I., Mandić, V.,& Bijelić, Z.. (2022). Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia, 17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793
Krnjaja V, Stanković S, Obradović A, Nikolić M, Savić I, Mandić V, Bijelić Z. Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia. 2022;:17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Savić, Iva, Mandić, Violeta, Bijelić, Zorica, "Fusarium and deoxynivalenol contamination of durum wheat lines kernels" in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia (2022):17-17,
https://hdl.handle.net/21.15107/rcub_ristocar_793 .

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