Quality and fertility of extended boar semen after prolonged transport
Authors
Barna, TomislavApić, Jelena
Milovanović, Aleksandar
Maksimović, Nevena

Mašić, Aleksandar
Lazarević, Marina

Pavlović, Miloš
Article (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membran...e permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome
Keywords:
Import, semen, boars, computer-assisted sperm analyses (CASA), flow cytometrySource:
Turkish Journal of Veterinary and Animal Sciences, 2021, 45, 5, 920-929Publisher:
- TUBITAK
Funding / projects:
Collections
Institution/Community
RIStocarTY - JOUR AU - Barna, Tomislav AU - Apić, Jelena AU - Milovanović, Aleksandar AU - Maksimović, Nevena AU - Mašić, Aleksandar AU - Lazarević, Marina AU - Pavlović, Miloš PY - 2021 UR - http://r.istocar.bg.ac.rs/handle/123456789/762 AB - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome PB - TUBITAK T2 - Turkish Journal of Veterinary and Animal Sciences T1 - Quality and fertility of extended boar semen after prolonged transport VL - 45 IS - 5 SP - 920 EP - 929 DO - 10.3906/vet-2012-63 ER -
@article{ author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Marina and Pavlović, Miloš", year = "2021", abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome", publisher = "TUBITAK", journal = "Turkish Journal of Veterinary and Animal Sciences", title = "Quality and fertility of extended boar semen after prolonged transport", volume = "45", number = "5", pages = "920-929", doi = "10.3906/vet-2012-63" }
Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences TUBITAK., 45(5), 920-929. https://doi.org/10.3906/vet-2012-63
Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929. doi:10.3906/vet-2012-63 .
Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Marina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929, https://doi.org/10.3906/vet-2012-63 . .