The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membran...e permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome
TY - JOUR
AU - Barna, Tomislav
AU - Apić, Jelena
AU - Milovanović, Aleksandar
AU - Maksimović, Nevena
AU - Mašić, Aleksandar
AU - Lazarević, Marina
AU - Pavlović, Miloš
PY - 2021
UR - http://r.istocar.bg.ac.rs/handle/123456789/762
AB - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome
PB - TUBITAK
T2 - Turkish Journal of Veterinary and Animal Sciences
T1 - Quality and fertility of extended boar semen after prolonged transport
VL - 45
IS - 5
SP - 920
EP - 929
DO - 10.3906/vet-2012-63
ER -
@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Marina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome",
publisher = "TUBITAK",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/vet-2012-63"
}
Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
TUBITAK., 45(5), 920-929.
https://doi.org/10.3906/vet-2012-63
Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/vet-2012-63 .
Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Marina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/vet-2012-63 . .
,