Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation
Аутори
Serafimov, MariosMetodiev, Nikola
Kanzova, Hristina
Karadjova, Veronika
Cekić, Bogdan
Andreeva, Madlena
Остала ауторства
Shishkov, StoyanКонференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of the present study was to determine a possible relationship between sperm
parameters and activity of creatinine kinase (CK) in the process of cryopreservation.
To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5
years, were examined. The rams were breeding under the same conditions of feeding,
growing and sexual use. Ejaculates were obtained via the artificial vagina method
and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined
by a computer assisted semen analysis (CASA) to determine total motility, velocity
parameters, and morphological status. The intracellular and extracellular activity of
CK were determined spectrophotometrically. After sperm evaluation, the ejaculates
were frozen using Cassou sequin technology and the same parameters were analyzed
after thawing the samples. From the obtained results we found a 26% decrease in
sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL ...and
BCF also decreased significantly after thawing (P≤0.01), but an increase was found
in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation
also increased the percentage of abnormal sperm by 17%. A higher intracellular CK
activity, which decreased significantly after freeze-thawing (P≤0.001) was measured.
In conclusion, cryopreservation decreased sperm motility and increased the percentage
of abnormal sperm (mostly with tail damage), but we did not establish a relationship
between linear the velocity parameters LIN, STR and WOB, sperm morphological
status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm
quality in the process of cryopreservation.
Кључне речи:
creatinine kinase / sperm / ram / cryopreservationИзвор:
Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria, 11-2021, 205-205Издавач:
- Faculty of Biology, Sofia University, Bulgaria
Институција/група
RIStocarTY - CONF AU - Serafimov, Marios AU - Metodiev, Nikola AU - Kanzova, Hristina AU - Karadjova, Veronika AU - Cekić, Bogdan AU - Andreeva, Madlena PY - 2021-11 UR - http://r.istocar.bg.ac.rs/handle/123456789/741 AB - The aim of the present study was to determine a possible relationship between sperm parameters and activity of creatinine kinase (CK) in the process of cryopreservation. To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5 years, were examined. The rams were breeding under the same conditions of feeding, growing and sexual use. Ejaculates were obtained via the artificial vagina method and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined by a computer assisted semen analysis (CASA) to determine total motility, velocity parameters, and morphological status. The intracellular and extracellular activity of CK were determined spectrophotometrically. After sperm evaluation, the ejaculates were frozen using Cassou sequin technology and the same parameters were analyzed after thawing the samples. From the obtained results we found a 26% decrease in sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and BCF also decreased significantly after thawing (P≤0.01), but an increase was found in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation also increased the percentage of abnormal sperm by 17%. A higher intracellular CK activity, which decreased significantly after freeze-thawing (P≤0.001) was measured. In conclusion, cryopreservation decreased sperm motility and increased the percentage of abnormal sperm (mostly with tail damage), but we did not establish a relationship between linear the velocity parameters LIN, STR and WOB, sperm morphological status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm quality in the process of cryopreservation. PB - Faculty of Biology, Sofia University, Bulgaria C3 - Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria T1 - Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation SP - 205 EP - 205 UR - https://hdl.handle.net/21.15107/rcub_ristocar_741 ER -
@conference{ author = "Serafimov, Marios and Metodiev, Nikola and Kanzova, Hristina and Karadjova, Veronika and Cekić, Bogdan and Andreeva, Madlena", year = "2021-11", abstract = "The aim of the present study was to determine a possible relationship between sperm parameters and activity of creatinine kinase (CK) in the process of cryopreservation. To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5 years, were examined. The rams were breeding under the same conditions of feeding, growing and sexual use. Ejaculates were obtained via the artificial vagina method and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined by a computer assisted semen analysis (CASA) to determine total motility, velocity parameters, and morphological status. The intracellular and extracellular activity of CK were determined spectrophotometrically. After sperm evaluation, the ejaculates were frozen using Cassou sequin technology and the same parameters were analyzed after thawing the samples. From the obtained results we found a 26% decrease in sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and BCF also decreased significantly after thawing (P≤0.01), but an increase was found in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation also increased the percentage of abnormal sperm by 17%. A higher intracellular CK activity, which decreased significantly after freeze-thawing (P≤0.001) was measured. In conclusion, cryopreservation decreased sperm motility and increased the percentage of abnormal sperm (mostly with tail damage), but we did not establish a relationship between linear the velocity parameters LIN, STR and WOB, sperm morphological status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm quality in the process of cryopreservation.", publisher = "Faculty of Biology, Sofia University, Bulgaria", journal = "Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria", title = "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation", pages = "205-205", url = "https://hdl.handle.net/21.15107/rcub_ristocar_741" }
Serafimov, M., Metodiev, N., Kanzova, H., Karadjova, V., Cekić, B.,& Andreeva, M.. (2021-11). Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria Faculty of Biology, Sofia University, Bulgaria., 205-205. https://hdl.handle.net/21.15107/rcub_ristocar_741
Serafimov M, Metodiev N, Kanzova H, Karadjova V, Cekić B, Andreeva M. Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria. 2021;:205-205. https://hdl.handle.net/21.15107/rcub_ristocar_741 .
Serafimov, Marios, Metodiev, Nikola, Kanzova, Hristina, Karadjova, Veronika, Cekić, Bogdan, Andreeva, Madlena, "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation" in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria (2021-11):205-205, https://hdl.handle.net/21.15107/rcub_ristocar_741 .