The aim of this study was to evaluate fungal infection, with a
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F.
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13)
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars
with a frequency of 0.5%. The frequency of DON po...sitive samples was 100%. A
statistically significant difference in DON level was found between the two tested
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in
the tested seed of Italian ryegrass indicate on potentially significant participation of
DON in FHB pathogenesis, as well as a potential risk for the quality seed
production, feed safety and the food chain in general. In Serbia, this is the first
report about Fusarium infection and DON presence in ryegrass seed.
Keywords:
Fusarium spp. / deoxynivalenol / Italian ryegrass
Source:
Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631, 2021, 622-631
TY - CONF
AU - Krnjaja, Vesna
AU - Mandić, Violeta
AU - Bijelić, Zorica
AU - Stanković, Slavica
AU - Nikolić, Milica
AU - Vasić, Tanja
AU - Delić, Nikola
PY - 2021
UR - http://r.istocar.bg.ac.rs/handle/123456789/738
AB - The aim of this study was to evaluate fungal infection, with a
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F.
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13)
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A
statistically significant difference in DON level was found between the two tested
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in
the tested seed of Italian ryegrass indicate on potentially significant participation of
DON in FHB pathogenesis, as well as a potential risk for the quality seed
production, feed safety and the food chain in general. In Serbia, this is the first
report about Fusarium infection and DON presence in ryegrass seed.
C3 - Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631
T1 - Fusarium spp. and deoxynivalenol contamination of ryegrass seeds
SP - 622
EP - 631
UR - https://hdl.handle.net/21.15107/rcub_ristocar_738
ER -
@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Nikolić, Milica and Vasić, Tanja and Delić, Nikola",
year = "2021",
abstract = "The aim of this study was to evaluate fungal infection, with a
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F.
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13)
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A
statistically significant difference in DON level was found between the two tested
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in
the tested seed of Italian ryegrass indicate on potentially significant participation of
DON in FHB pathogenesis, as well as a potential risk for the quality seed
production, feed safety and the food chain in general. In Serbia, this is the first
report about Fusarium infection and DON presence in ryegrass seed.",
journal = "Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631",
title = "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds",
pages = "622-631",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_738"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Nikolić, M., Vasić, T.,& Delić, N.. (2021). Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631, 622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Nikolić M, Vasić T, Delić N. Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631. 2021;:622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738 .
Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Nikolić, Milica, Vasić, Tanja, Delić, Nikola, "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds" in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631 (2021):622-631,
https://hdl.handle.net/21.15107/rcub_ristocar_738 .
