Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms
Authors
Stanojković, Aleksandar
Radović, Čedomir

Stanojković-Sebić, Aleksandra
Gogić, Marija

Mandić, Violeta

Nišavić, Jakov
Petričević, Maja

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The goal of this study was to determine antimicrobial
susceptibility of 34 Streptococcus suis strains isolated from healthy and diseased
pigs from two pig farms. Disk diffusion was the method used to asses antibacterial
susceptibility of S. suis according to CLSI (Clinical and Laboratory Standards
Institute) and EUCAST standard protocols. Overnight cultures of S. suis grown on
Colombia 5% blood agar (HIMEDIA, India) were suspended in Todd Hewitt broth
(Oxoid Limited, England) and suspension was adjusted to 0,5 McFarland standard.
Using sterile swabs suspension was spreaded to Mueller Hinton agar supplemented
with 5% defibrinated sheep blood, after which standadized discs containing
antimicrobial substances were placed on the agar surface. Plates were incubated at
37 °C in aerobic conditions usually for 18 hours, but no more than 24 hours after
which diameter of S. suis inhibition zones were measured. Among 34 isolated S.
suis strains all were resistant to tetracycline (TET)..., lincomycin (L) and
clindamycin (CC). Susceptibility was very low to sulfamethoxazole-trimethoprim
(SXT) combination (94,1% resistance), 94,1% of strains were sensitive to
penicillin G (P), amoxicillin (AMX), erythromycin (E), enrofloxacin (ENR) and
azithromycin (AZM) while sensitivity to cephalosporins of the first (cephalexin-
CN), third (cefotaxime – CTX, ceftriaxone - CRO) and fourth ( cefepime-FEP)
generation, as well as the sensitivity to florfenicol (FFC) and vancomycin (VAN)
was absolute (100%). Therefore we can conclude that cephalosporins are very
reasonable choice in treatment of human cases of S. suis meningitis but also as a
treatment of choice for the S .suis pig septicemia.
Keywords:
Streptococcus suis / antimicrobial susceptibility / pig / antibioticsSource:
Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade, 2019, 148-154Publisher:
- Institute for Animal Husbandry, Belgrade-Zemun
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RIStocarTY - CONF AU - Stanojković, Aleksandar AU - Radović, Čedomir AU - Stanojković-Sebić, Aleksandra AU - Gogić, Marija AU - Mandić, Violeta AU - Nišavić, Jakov AU - Petričević, Maja PY - 2019 UR - http://r.istocar.bg.ac.rs/handle/123456789/676 AB - The goal of this study was to determine antimicrobial susceptibility of 34 Streptococcus suis strains isolated from healthy and diseased pigs from two pig farms. Disk diffusion was the method used to asses antibacterial susceptibility of S. suis according to CLSI (Clinical and Laboratory Standards Institute) and EUCAST standard protocols. Overnight cultures of S. suis grown on Colombia 5% blood agar (HIMEDIA, India) were suspended in Todd Hewitt broth (Oxoid Limited, England) and suspension was adjusted to 0,5 McFarland standard. Using sterile swabs suspension was spreaded to Mueller Hinton agar supplemented with 5% defibrinated sheep blood, after which standadized discs containing antimicrobial substances were placed on the agar surface. Plates were incubated at 37 °C in aerobic conditions usually for 18 hours, but no more than 24 hours after which diameter of S. suis inhibition zones were measured. Among 34 isolated S. suis strains all were resistant to tetracycline (TET), lincomycin (L) and clindamycin (CC). Susceptibility was very low to sulfamethoxazole-trimethoprim (SXT) combination (94,1% resistance), 94,1% of strains were sensitive to penicillin G (P), amoxicillin (AMX), erythromycin (E), enrofloxacin (ENR) and azithromycin (AZM) while sensitivity to cephalosporins of the first (cephalexin- CN), third (cefotaxime – CTX, ceftriaxone - CRO) and fourth ( cefepime-FEP) generation, as well as the sensitivity to florfenicol (FFC) and vancomycin (VAN) was absolute (100%). Therefore we can conclude that cephalosporins are very reasonable choice in treatment of human cases of S. suis meningitis but also as a treatment of choice for the S .suis pig septicemia. PB - Institute for Animal Husbandry, Belgrade-Zemun C3 - Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade T1 - Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms SP - 148 EP - 154 UR - https://hdl.handle.net/21.15107/rcub_ristocar_676 ER -
@conference{ author = "Stanojković, Aleksandar and Radović, Čedomir and Stanojković-Sebić, Aleksandra and Gogić, Marija and Mandić, Violeta and Nišavić, Jakov and Petričević, Maja", year = "2019", abstract = "The goal of this study was to determine antimicrobial susceptibility of 34 Streptococcus suis strains isolated from healthy and diseased pigs from two pig farms. Disk diffusion was the method used to asses antibacterial susceptibility of S. suis according to CLSI (Clinical and Laboratory Standards Institute) and EUCAST standard protocols. Overnight cultures of S. suis grown on Colombia 5% blood agar (HIMEDIA, India) were suspended in Todd Hewitt broth (Oxoid Limited, England) and suspension was adjusted to 0,5 McFarland standard. Using sterile swabs suspension was spreaded to Mueller Hinton agar supplemented with 5% defibrinated sheep blood, after which standadized discs containing antimicrobial substances were placed on the agar surface. Plates were incubated at 37 °C in aerobic conditions usually for 18 hours, but no more than 24 hours after which diameter of S. suis inhibition zones were measured. Among 34 isolated S. suis strains all were resistant to tetracycline (TET), lincomycin (L) and clindamycin (CC). Susceptibility was very low to sulfamethoxazole-trimethoprim (SXT) combination (94,1% resistance), 94,1% of strains were sensitive to penicillin G (P), amoxicillin (AMX), erythromycin (E), enrofloxacin (ENR) and azithromycin (AZM) while sensitivity to cephalosporins of the first (cephalexin- CN), third (cefotaxime – CTX, ceftriaxone - CRO) and fourth ( cefepime-FEP) generation, as well as the sensitivity to florfenicol (FFC) and vancomycin (VAN) was absolute (100%). Therefore we can conclude that cephalosporins are very reasonable choice in treatment of human cases of S. suis meningitis but also as a treatment of choice for the S .suis pig septicemia.", publisher = "Institute for Animal Husbandry, Belgrade-Zemun", journal = "Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade", title = "Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms", pages = "148-154", url = "https://hdl.handle.net/21.15107/rcub_ristocar_676" }
Stanojković, A., Radović, Č., Stanojković-Sebić, A., Gogić, M., Mandić, V., Nišavić, J.,& Petričević, M.. (2019). Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade Institute for Animal Husbandry, Belgrade-Zemun., 148-154. https://hdl.handle.net/21.15107/rcub_ristocar_676
Stanojković A, Radović Č, Stanojković-Sebić A, Gogić M, Mandić V, Nišavić J, Petričević M. Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade. 2019;:148-154. https://hdl.handle.net/21.15107/rcub_ristocar_676 .
Stanojković, Aleksandar, Radović, Čedomir, Stanojković-Sebić, Aleksandra, Gogić, Marija, Mandić, Violeta, Nišavić, Jakov, Petričević, Maja, "Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms" in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade (2019):148-154, https://hdl.handle.net/21.15107/rcub_ristocar_676 .