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dc.contributorRadović, Čedomir
dc.creatorAndreeva, Madlena
dc.creatorMetodiev, Nikola
dc.creatorCekić, Bogdan
dc.creatorStefanov, Rossen
dc.date.accessioned2020-01-10T06:12:34Z
dc.date.available2020-01-10T06:12:34Z
dc.date.issued2019
dc.identifier.isbn978-86-82431-76-3
dc.identifier.urihttp://r.istocar.bg.ac.rs/handle/123456789/671
dc.description.abstractThe aim of the study was to evaluate how the process of cryopreservation influenced the morphological status of ram sperm. The experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed – 15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse E200, Japan) by staining with SpermBlue (Microptic, Spain), performed according to the manufacturer's protocol, and observed under bright field with magnification of ×40. Cryopreservation of semen straws was done by the method of Cassou (1964). After cryopreservation, the percentage of spermatozoa with normal morphology decreased and those with abnormal morphology increased. This difference was more pronounced at rams from SPBM compared to SB rams. After freezing, the percentage of spermatozoa with damage to the head at rams from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low temperatures of cryopreservation had a detrimental effect to the rams’ sperm structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.en
dc.language.isoensr
dc.publisherInstitute for Animal Husbandry, Belgrade-Zemunsr
dc.relationNational Scientific Program for young scientists and postdoctoral fellows, funded by the Bulgarian Ministry of Education and Science (MES) with РМС № 577 (17.08.2018)sr
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceProceedings of the 12th International Symposium Modern Trends in Livestock Productionsr
dc.subjectramsr
dc.subjectcryopreservationsr
dc.subjectspermatozoasr
dc.subjectmorphology statussr
dc.titleStudy of the effects of low temperatures on the morphological status of ram spermatozoaen
dc.typeconferenceObjectsr
dc.rights.licenseBYsr
dcterms.abstractСтефанов, Россен; Aндреева, Мадлена; Методиев, Никола; Цекић, Богдан;
dc.citation.spage373
dc.citation.epage381
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_ristocar_671
dc.identifier.fulltexthttp://r.istocar.bg.ac.rs/bitstream/id/3526/bitstream_3526.pdf
dc.type.versionpublishedVersionsr


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Приказ основних података о документу