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Study of the effects of low temperatures on the morphological status of ram spermatozoa

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2019
STUDY OF THE EFFECTS OF LOW TEMPERATURES ON THE MORPHOLOGICAL STATUS OF RAM SPERMATOZOA (428.9Kb)
Authors
Andreeva, Madlena
Metodiev, Nikola
Cekić, Bogdan
Stefanov, Rossen
Contributors
Radović, Čedomir
Conference object (Published version)
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Abstract
The aim of the study was to evaluate how the process of cryopreservation influenced the morphological status of ram sperm. The experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed – 15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse E200, Japan) by staining with SpermBlue (Microptic, Spain), performed according to the manufacturer's protocol, and observed under bright field with magnification of ×40. Cryopreservation of semen straws was done by the method of Cassou (1964). After cryopreservation, the percentage of spermatozoa with normal morphology decreased and those with abnormal morphology increased. This difference was more pronounced at rams from SPBM compared to SB rams. After freezin...g, the percentage of spermatozoa with damage to the head at rams from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low temperatures of cryopreservation had a detrimental effect to the rams’ sperm structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.

Keywords:
ram / cryopreservation / spermatozoa / morphology status
Source:
Proceedings of the 12th International Symposium Modern Trends in Livestock Production, 2019, 373-381
Publisher:
  • Institute for Animal Husbandry, Belgrade-Zemun
Funding / projects:
  • National Scientific Program for young scientists and postdoctoral fellows, funded by the Bulgarian Ministry of Education and Science (MES) with РМС № 577 (17.08.2018)

ISBN: 978-86-82431-76-3

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_ristocar_671
URI
http://r.istocar.bg.ac.rs/handle/123456789/671
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
RIStocar
TY  - CONF
AU  - Andreeva, Madlena
AU  - Metodiev, Nikola
AU  - Cekić, Bogdan
AU  - Stefanov, Rossen
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/671
AB  - The aim  of  the  study  was  to  evaluate how  the  process  of  cryopreservation   influenced   the   morphological   status   of   ram   sperm.   The   experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed –   15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse  E200,  Japan)  by  staining  with  SpermBlue  (Microptic,  Spain),  performed according  to  the  manufacturer's  protocol,  and  observed  under  bright  field  with  magnification  of  ×40.  Cryopreservation  of  semen  straws  was  done  by  the  method  of  Cassou  (1964).  After  cryopreservation,  the  percentage  of  spermatozoa  with  normal  morphology  decreased  and  those  with  abnormal  morphology  increased.  This difference was more pronounced at rams from SPBM compared to SB rams. After  freezing,  the  percentage  of  spermatozoa  with  damage  to  the  head  at  rams  from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low  temperatures  of  cryopreservation  had a  detrimental  effect  to  the  rams’  sperm  structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings of the 12th International Symposium Modern Trends in Livestock Production
T1  - Study of the effects of low temperatures on the morphological status of ram spermatozoa
SP  - 373
EP  - 381
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_671
ER  - 
@conference{
author = "Andreeva, Madlena and Metodiev, Nikola and Cekić, Bogdan and Stefanov, Rossen",
year = "2019",
abstract = "The aim  of  the  study  was  to  evaluate how  the  process  of  cryopreservation   influenced   the   morphological   status   of   ram   sperm.   The   experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed –   15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse  E200,  Japan)  by  staining  with  SpermBlue  (Microptic,  Spain),  performed according  to  the  manufacturer's  protocol,  and  observed  under  bright  field  with  magnification  of  ×40.  Cryopreservation  of  semen  straws  was  done  by  the  method  of  Cassou  (1964).  After  cryopreservation,  the  percentage  of  spermatozoa  with  normal  morphology  decreased  and  those  with  abnormal  morphology  increased.  This difference was more pronounced at rams from SPBM compared to SB rams. After  freezing,  the  percentage  of  spermatozoa  with  damage  to  the  head  at  rams  from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low  temperatures  of  cryopreservation  had a  detrimental  effect  to  the  rams’  sperm  structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings of the 12th International Symposium Modern Trends in Livestock Production",
title = "Study of the effects of low temperatures on the morphological status of ram spermatozoa",
pages = "373-381",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_671"
}
Andreeva, M., Metodiev, N., Cekić, B.,& Stefanov, R.. (2019). Study of the effects of low temperatures on the morphological status of ram spermatozoa. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production
Institute for Animal Husbandry, Belgrade-Zemun., 373-381.
https://hdl.handle.net/21.15107/rcub_ristocar_671
Andreeva M, Metodiev N, Cekić B, Stefanov R. Study of the effects of low temperatures on the morphological status of ram spermatozoa. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production. 2019;:373-381.
https://hdl.handle.net/21.15107/rcub_ristocar_671 .
Andreeva, Madlena, Metodiev, Nikola, Cekić, Bogdan, Stefanov, Rossen, "Study of the effects of low temperatures on the morphological status of ram spermatozoa" in Proceedings of the 12th International Symposium Modern Trends in Livestock Production (2019):373-381,
https://hdl.handle.net/21.15107/rcub_ristocar_671 .

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