Due to the ecological concerns and preservation of genetic
resources, the characterization of genetic structure of the brown trout (Salmo trutta)
was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of
10 individuals, and after that molecular PCR-RFLP methods were used for
distinguishing between Atlantic and Danubian lineages based on control region of
the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the
nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated
that out of 10 individuals included in this study five belonged to the allochtonous
Atlantic lineage and remaining five belonged to the native Danubian lineage of
brown trout. However, results of molecular analyses showed a high percentage of
allochthonous genes among the individuals, which confirms the hybridization
between these two lineages. Also, the results showed that the selection based on the
phenotype is not adequate. In order to continue with pr...oper broodstock
management, it is necessary to eliminate allochtonous individuals of the Atlantic
lineage from the broodstock.
TY - JOUR
AU - Molerović, Nikola
AU - Raskovic, Bozidar
AU - Đedović, Radica
AU - Ostojić Andrić, Dušica
AU - Marković, Zoran
AU - Marić, Saša
PY - 2019
UR - http://r.istocar.bg.ac.rs/handle/123456789/657
AB - Due to the ecological concerns and preservation of genetic
resources, the characterization of genetic structure of the brown trout (Salmo trutta)
was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of
10 individuals, and after that molecular PCR-RFLP methods were used for
distinguishing between Atlantic and Danubian lineages based on control region of
the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the
nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated
that out of 10 individuals included in this study five belonged to the allochtonous
Atlantic lineage and remaining five belonged to the native Danubian lineage of
brown trout. However, results of molecular analyses showed a high percentage of
allochthonous genes among the individuals, which confirms the hybridization
between these two lineages. Also, the results showed that the selection based on the
phenotype is not adequate. In order to continue with proper broodstock
management, it is necessary to eliminate allochtonous individuals of the Atlantic
lineage from the broodstock.
PB - Institute for Animal Husbandry, Belgrade-Zemun
T2 - Biotechnology in Animal Husbandry
T1 - Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia
VL - 35
IS - 3
SP - 289
EP - 299
DO - https://doi.org/10.2298/BAH1903289M
ER -
@article{
author = "Molerović, Nikola and Raskovic, Bozidar and Đedović, Radica and Ostojić Andrić, Dušica and Marković, Zoran and Marić, Saša",
year = "2019",
abstract = "Due to the ecological concerns and preservation of genetic
resources, the characterization of genetic structure of the brown trout (Salmo trutta)
was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of
10 individuals, and after that molecular PCR-RFLP methods were used for
distinguishing between Atlantic and Danubian lineages based on control region of
the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the
nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated
that out of 10 individuals included in this study five belonged to the allochtonous
Atlantic lineage and remaining five belonged to the native Danubian lineage of
brown trout. However, results of molecular analyses showed a high percentage of
allochthonous genes among the individuals, which confirms the hybridization
between these two lineages. Also, the results showed that the selection based on the
phenotype is not adequate. In order to continue with proper broodstock
management, it is necessary to eliminate allochtonous individuals of the Atlantic
lineage from the broodstock.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia",
volume = "35",
number = "3",
pages = "289-299",
doi = "https://doi.org/10.2298/BAH1903289M"
}
Molerović, N., Raskovic, B., Đedović, R., Ostojić Andrić, D., Marković, Z.,& Marić, S.. (2019). Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 35(3), 289-299.
https://doi.org/https://doi.org/10.2298/BAH1903289M
Molerović N, Raskovic B, Đedović R, Ostojić Andrić D, Marković Z, Marić S. Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia. in Biotechnology in Animal Husbandry. 2019;35(3):289-299.
doi:https://doi.org/10.2298/BAH1903289M .
Molerović, Nikola, Raskovic, Bozidar, Đedović, Radica, Ostojić Andrić, Dušica, Marković, Zoran, Marić, Saša, "Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia" in Biotechnology in Animal Husbandry, 35, no. 3 (2019):289-299,
https://doi.org/https://doi.org/10.2298/BAH1903289M . .
