Frequency of κ-casein and β-lactoglobulin genotypes in daughters of five simmental bull sires

Abstract

The aim of this study was to determine the frequency of the κ-casein and β-lactoglobulin genotypes of the daughters of 5 Simmental bulls (sires) used for insemination in Serbia. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Cows from which blood samples were taken were the daughters of the following bulls: Rorb B033, Dionis B52, Val B064, Rondi B029 and Res 1433. In all daughters of studied bulls, the presence of all three genotypes was determined, except for daughters of bull sire Res B1433, where the absence of genotype BB was determined. In addition to determining the frequency of genotypes and alleles for both milk protein fractions, based on the χ2 test, a link between the sire and the presence of genotypes was determined, and in both cases a statistically significant relationship (p≤0.001) was found.

Cilj ovog istraživanja bio je da se utvrdi frekvencija genotipova κ-kazeina i β-laktoglobulina ćerki 5 bikova (očeva) sementalske rase koji se koriste za osemenjavanje u Srbiji. Uzorci krvi uzeti su iz ukupno 157 krava simentalske rase u Topličkom i Rasinskom okrugu. Krave od kojih su uzeti uzorci krvi bile su ćerke sledećih bikova: Rorb B033, Dionis B52, Val B064, Rondi B029 i Res 1433. Kod svih ćerki, ispitivanih bikova, utvrđeno je prisustvo sva tri genotipa, osim kod ćerki bika Res B1433, kod kojih je utvrđeno odsustvo genotipa BB. Pored određivanja frekvencija genotipova i alela za obe frakcije proteina mleka, na osnovu χ2 testa urađeno je ispitivanje veze između očeva i zastupljenosti genotipova, i u oba slučaja je utvrđeno da postoji statistički značajna veza (p≤0,001).