Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia

2018
Authors
Nikšić, Dragan
Pantelić, Vlada

Ostojić Andrić, Dušica

Perišić, Predrag
Petričević, Veselin

Lazarević, Marina

Petričević, Maja

Article (Published version)
Metadata
Show full item recordAbstract
The objective of this study was to determine the frequency of the kappa-casein and beta-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA kappa-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and beta-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for beta-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00...% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population.
Keywords:
beta-lactoglobulin / kappa-casein / polymorphism / PCR-RFLP / Simmental breedSource:
Genetika-Belgrade, 2018, 50, 2, 659-668Publisher:
- Serbian Genetics Society, Belgrade
Funding / projects:
- Development of vegetable cultivars and hybrids intended for outdoor and indoor production (RS-31030)
DOI: 10.2298/GENSR1802659N
ISSN: 0534-0012