Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia
2017
Аутори
Vasić, TanjaJevremović, Darko
Krnjaja, Vesna
Leposavić, Aleksandar
Anđelković, S.
Živković, Sanja
Paunović, Svetlana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.
Кључне речи:
Corylus avellana L. / leaf blight / beta-tubulin / ITS / EF1-alphaИзвор:
Spanish Journal of Agricultural Research, 2017, 15, 3, e10SC02-Издавач:
- Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
Финансирање / пројекти:
- Fruit Research Institute; Institute for Forage Crops (Serbia)
DOI: 10.5424/sjar/2017153-11297
ISSN: 1695-971X
WoS: 000414947500017
Scopus: 2-s2.0-85033378805
Институција/група
RIStocarTY - JOUR AU - Vasić, Tanja AU - Jevremović, Darko AU - Krnjaja, Vesna AU - Leposavić, Aleksandar AU - Anđelković, S. AU - Živković, Sanja AU - Paunović, Svetlana PY - 2017 UR - http://r.istocar.bg.ac.rs/handle/123456789/531 AB - In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut. PB - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid T2 - Spanish Journal of Agricultural Research T1 - Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia VL - 15 IS - 3 SP - e10SC02 DO - 10.5424/sjar/2017153-11297 ER -
@article{ author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Leposavić, Aleksandar and Anđelković, S. and Živković, Sanja and Paunović, Svetlana", year = "2017", abstract = "In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.", publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid", journal = "Spanish Journal of Agricultural Research", title = "Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia", volume = "15", number = "3", pages = "e10SC02", doi = "10.5424/sjar/2017153-11297" }
Vasić, T., Jevremović, D., Krnjaja, V., Leposavić, A., Anđelković, S., Živković, S.,& Paunović, S.. (2017). Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia. in Spanish Journal of Agricultural Research Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 15(3), e10SC02. https://doi.org/10.5424/sjar/2017153-11297
Vasić T, Jevremović D, Krnjaja V, Leposavić A, Anđelković S, Živković S, Paunović S. Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia. in Spanish Journal of Agricultural Research. 2017;15(3):e10SC02. doi:10.5424/sjar/2017153-11297 .
Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Leposavić, Aleksandar, Anđelković, S., Živković, Sanja, Paunović, Svetlana, "Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia" in Spanish Journal of Agricultural Research, 15, no. 3 (2017):e10SC02, https://doi.org/10.5424/sjar/2017153-11297 . .