Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

2017
Authors
Obradović, AnaStanković, Slavica Ž.
Krnjaja, Vesna

Nikolic, Ana
Ignjatović-Micić, Dragana
Stepanović, Jelena
Duduk, Bojan
Article (Published version)
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Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respecti...vely. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
Keywords:
Fusarium graminearum / trichothecene chemotype / NIV / 15-ADON / 3ADONSource:
Genetika-Belgrade, 2017, 49, 1, 355-364Publisher:
- Serbian Genetics Society, Belgrade
Funding / projects:
DOI: 10.2298/GENSR1701355O
ISSN: 0534-0012
WoS: 000405684200030
Scopus: 2-s2.0-85026236193
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RIStocarTY - JOUR AU - Obradović, Ana AU - Stanković, Slavica Ž. AU - Krnjaja, Vesna AU - Nikolic, Ana AU - Ignjatović-Micić, Dragana AU - Stepanović, Jelena AU - Duduk, Bojan PY - 2017 UR - http://r.istocar.bg.ac.rs/handle/123456789/526 AB - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions. PB - Serbian Genetics Society, Belgrade T2 - Genetika-Belgrade T1 - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia VL - 49 IS - 1 SP - 355 EP - 364 DO - 10.2298/GENSR1701355O ER -
@article{ author = "Obradović, Ana and Stanković, Slavica Ž. and Krnjaja, Vesna and Nikolic, Ana and Ignjatović-Micić, Dragana and Stepanović, Jelena and Duduk, Bojan", year = "2017", abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.", publisher = "Serbian Genetics Society, Belgrade", journal = "Genetika-Belgrade", title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia", volume = "49", number = "1", pages = "355-364", doi = "10.2298/GENSR1701355O" }
Obradović, A., Stanković, S. Ž., Krnjaja, V., Nikolic, A., Ignjatović-Micić, D., Stepanović, J.,& Duduk, B.. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade Serbian Genetics Society, Belgrade., 49(1), 355-364. https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković SŽ, Krnjaja V, Nikolic A, Ignjatović-Micić D, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade. 2017;49(1):355-364. doi:10.2298/GENSR1701355O .
Obradović, Ana, Stanković, Slavica Ž., Krnjaja, Vesna, Nikolic, Ana, Ignjatović-Micić, Dragana, Stepanović, Jelena, Duduk, Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" in Genetika-Belgrade, 49, no. 1 (2017):355-364, https://doi.org/10.2298/GENSR1701355O . .