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The application of PCR based methods in diagnostics of some viral infections of swine

Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja

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2016
485.pdf (216.7Kb)
Authors
Nišavić, Jakov
Milić, Nenad
Zorić, Andrea
Bojkovski, Jovan
Stanojković, Aleksandar
Article (Published version)
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Abstract
Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using stand...ard virological methods.

Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije... se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.

Keywords:
swine / virus / PCR / real-time PCR
Source:
Biotechnology in Animal Husbandry, 2016, 32, 4, 321-329
Publisher:
  • Institute for Animal Husbandry, Belgrade
Funding / projects:
  • Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens (RS-31008)

DOI: 10.2298/bah1604321N

ISSN: 1450-9156

[ Google Scholar ]
URI
http://r.istocar.bg.ac.rs/handle/123456789/487
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
RIStocar
TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Zorić, Andrea
AU  - Bojkovski, Jovan
AU  - Stanojković, Aleksandar
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/487
AB  - Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods.
AB  - Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - The application of PCR based methods in diagnostics of some viral infections of swine
T1  - Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja
VL  - 32
IS  - 4
SP  - 321
EP  - 329
DO  - 10.2298/bah1604321N
ER  - 
@article{
author = "Nišavić, Jakov and Milić, Nenad and Zorić, Andrea and Bojkovski, Jovan and Stanojković, Aleksandar",
year = "2016",
abstract = "Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods., Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "The application of PCR based methods in diagnostics of some viral infections of swine, Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja",
volume = "32",
number = "4",
pages = "321-329",
doi = "10.2298/bah1604321N"
}
Nišavić, J., Milić, N., Zorić, A., Bojkovski, J.,& Stanojković, A.. (2016). The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(4), 321-329.
https://doi.org/10.2298/bah1604321N
Nišavić J, Milić N, Zorić A, Bojkovski J, Stanojković A. The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry. 2016;32(4):321-329.
doi:10.2298/bah1604321N .
Nišavić, Jakov, Milić, Nenad, Zorić, Andrea, Bojkovski, Jovan, Stanojković, Aleksandar, "The application of PCR based methods in diagnostics of some viral infections of swine" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):321-329,
https://doi.org/10.2298/bah1604321N . .

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