Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia

2016
Authors
Maletić, MilanAleksić, Nevenka

Vejnović, Branislav

Nikšić, Dragan
Kulić, Milan
Djukić, Božidar
Ćirković, Dragan
article (publishedVersion)
Metadata
Show full item recordAbstract
The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype w...as not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.
Keywords:
kappa-casein / beta-lactoglobulin / polymorphism / PCR-RFLP / Holstein-Friesian / Busha cattleSource:
Mljekarstvo, 2016, 66, 3, 198-205Publisher:
- Croatian Dairy Union, Zagreb
Funding / projects:
- info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// (RS-46002)
DOI: 10.15567/mljekarstvo.2016.0304
ISSN: 0026-704X