Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia

2016
Authors
Maletić, MilanAleksić, Nevenka

Vejnović, Branislav

Nikšić, Dragan

Kulić, Milan
Djukić, Božidar
Ćirković, Dragan
Article (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype w...as not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.
Keywords:
kappa-casein / beta-lactoglobulin / polymorphism / PCR-RFLP / Holstein-Friesian / Busha cattleSource:
Mljekarstvo, 2016, 66, 3, 198-205Publisher:
- Croatian Dairy Union, Zagreb
Funding / projects:
DOI: 10.15567/mljekarstvo.2016.0304
ISSN: 0026-704X
WoS: 000379483400004
Scopus: 2-s2.0-84979084593
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RIStocarTY - JOUR AU - Maletić, Milan AU - Aleksić, Nevenka AU - Vejnović, Branislav AU - Nikšić, Dragan AU - Kulić, Milan AU - Djukić, Božidar AU - Ćirković, Dragan PY - 2016 UR - http://r.istocar.bg.ac.rs/handle/123456789/483 AB - The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows. PB - Croatian Dairy Union, Zagreb T2 - Mljekarstvo T1 - Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia VL - 66 IS - 3 SP - 198 EP - 205 DO - 10.15567/mljekarstvo.2016.0304 ER -
@article{ author = "Maletić, Milan and Aleksić, Nevenka and Vejnović, Branislav and Nikšić, Dragan and Kulić, Milan and Djukić, Božidar and Ćirković, Dragan", year = "2016", abstract = "The aim of this study was to determine the distribution of kappa-casein (kappa-CN) and beta-lactoglobulin (beta-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of kappa-CN and beta-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that kappa-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for kappa-CN. As for beta-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for beta-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and beta-lactoglobulin) than HF cows.", publisher = "Croatian Dairy Union, Zagreb", journal = "Mljekarstvo", title = "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia", volume = "66", number = "3", pages = "198-205", doi = "10.15567/mljekarstvo.2016.0304" }
Maletić, M., Aleksić, N., Vejnović, B., Nikšić, D., Kulić, M., Djukić, B.,& Ćirković, D.. (2016). Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo Croatian Dairy Union, Zagreb., 66(3), 198-205. https://doi.org/10.15567/mljekarstvo.2016.0304
Maletić M, Aleksić N, Vejnović B, Nikšić D, Kulić M, Djukić B, Ćirković D. Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia. in Mljekarstvo. 2016;66(3):198-205. doi:10.15567/mljekarstvo.2016.0304 .
Maletić, Milan, Aleksić, Nevenka, Vejnović, Branislav, Nikšić, Dragan, Kulić, Milan, Djukić, Božidar, Ćirković, Dragan, "Polymorphism of kappa-casein and beta-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia" in Mljekarstvo, 66, no. 3 (2016):198-205, https://doi.org/10.15567/mljekarstvo.2016.0304 . .