Porcine stress syndrome (PSS) is one kind of molecular genetics defect which will cause malignant hyperthermia syndrome in pigs. It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for PSS. The aim of this study was to test the RYR1 genotype of 10 Mangalitsa pigs using a polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) technique, which is a reliable and simple method for RYR1 gene testing. Extraction of DNA was done by using hair follicles. The results showed that the RYR1 genotype of all the 10 porcine cases were negative. These results suggested that Mangalitsa pig could be one of the porcine breeds selectively bred for medical and clinically experiments.
Stres sindrom svinja (PSS - Porcine Stress Syndrome) pripada genetskim oboljenjima svinja i može da izazove sindrom maligne hipertermije. Utvrđeno je da je mutacija rijanodin receptora (RYR1) gena svinja glavni razlog za pojavu PSS-a. Cilj ovog ogleda bio je testiranje RYR1 gena na 10 svinja rase mangulica korišćenjem PCR-restriktivnog endonukleaza testa (PCR-RFLP), koji predstavlja jednostavnu i pouzdanu tehniku za testiranje RYR1 gena. Ekstrakcija DNK je obavljena korišćenjem korena dlake svinja. Rezultati ogleda potvrđuju da je RYR1 genotip kod svih 10 svinja bio negativan. Na osnovu napred navedenog može se zaključiti da bi mangulica mogla da bude jedna od genotipova koji se koriste za medicinska i klinička ispitivanja.
TY - JOUR
AU - Stanišić, Nikola
AU - Aleksić, S.
AU - Di, L.
AU - Stanimirović, Zoran
AU - Zhenhua, G.
AU - Petrović, M.
AU - Delić, Nikola
AU - Radović, Čedomir
AU - Parunović, Nenad
AU - Gogić, Marija
PY - 2012
UR - http://r.istocar.bg.ac.rs/handle/123456789/354
AB - Porcine stress syndrome (PSS) is one kind of molecular genetics defect which will cause malignant hyperthermia syndrome in pigs. It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for PSS. The aim of this study was to test the RYR1 genotype of 10 Mangalitsa pigs using a polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) technique, which is a reliable and simple method for RYR1 gene testing. Extraction of DNA was done by using hair follicles. The results showed that the RYR1 genotype of all the 10 porcine cases were negative. These results suggested that Mangalitsa pig could be one of the porcine breeds selectively bred for medical and clinically experiments.
AB - Stres sindrom svinja (PSS - Porcine Stress Syndrome) pripada genetskim oboljenjima svinja i može da izazove sindrom maligne hipertermije. Utvrđeno je da je mutacija rijanodin receptora (RYR1) gena svinja glavni razlog za pojavu PSS-a. Cilj ovog ogleda bio je testiranje RYR1 gena na 10 svinja rase mangulica korišćenjem PCR-restriktivnog endonukleaza testa (PCR-RFLP), koji predstavlja jednostavnu i pouzdanu tehniku za testiranje RYR1 gena. Ekstrakcija DNK je obavljena korišćenjem korena dlake svinja. Rezultati ogleda potvrđuju da je RYR1 genotip kod svih 10 svinja bio negativan. Na osnovu napred navedenog može se zaključiti da bi mangulica mogla da bude jedna od genotipova koji se koriste za medicinska i klinička ispitivanja.
PB - Institute for Animal Husbandry, Belgrade
T2 - Biotechnology in Animal Husbandry
T1 - Porcine stress syndrome (PSS) in Mangalitsa pigs
T1 - Prisustvo stres sindroma (PSS) kod svinja rase mangulica
VL - 28
IS - 4
SP - 873
EP - 878
DO - 10.2298/BAH1204873S
ER -
@article{
author = "Stanišić, Nikola and Aleksić, S. and Di, L. and Stanimirović, Zoran and Zhenhua, G. and Petrović, M. and Delić, Nikola and Radović, Čedomir and Parunović, Nenad and Gogić, Marija",
year = "2012",
abstract = "Porcine stress syndrome (PSS) is one kind of molecular genetics defect which will cause malignant hyperthermia syndrome in pigs. It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for PSS. The aim of this study was to test the RYR1 genotype of 10 Mangalitsa pigs using a polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) technique, which is a reliable and simple method for RYR1 gene testing. Extraction of DNA was done by using hair follicles. The results showed that the RYR1 genotype of all the 10 porcine cases were negative. These results suggested that Mangalitsa pig could be one of the porcine breeds selectively bred for medical and clinically experiments., Stres sindrom svinja (PSS - Porcine Stress Syndrome) pripada genetskim oboljenjima svinja i može da izazove sindrom maligne hipertermije. Utvrđeno je da je mutacija rijanodin receptora (RYR1) gena svinja glavni razlog za pojavu PSS-a. Cilj ovog ogleda bio je testiranje RYR1 gena na 10 svinja rase mangulica korišćenjem PCR-restriktivnog endonukleaza testa (PCR-RFLP), koji predstavlja jednostavnu i pouzdanu tehniku za testiranje RYR1 gena. Ekstrakcija DNK je obavljena korišćenjem korena dlake svinja. Rezultati ogleda potvrđuju da je RYR1 genotip kod svih 10 svinja bio negativan. Na osnovu napred navedenog može se zaključiti da bi mangulica mogla da bude jedna od genotipova koji se koriste za medicinska i klinička ispitivanja.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Porcine stress syndrome (PSS) in Mangalitsa pigs, Prisustvo stres sindroma (PSS) kod svinja rase mangulica",
volume = "28",
number = "4",
pages = "873-878",
doi = "10.2298/BAH1204873S"
}
Stanišić, N., Aleksić, S., Di, L., Stanimirović, Z., Zhenhua, G., Petrović, M., Delić, N., Radović, Č., Parunović, N.,& Gogić, M.. (2012). Porcine stress syndrome (PSS) in Mangalitsa pigs. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 28(4), 873-878.
https://doi.org/10.2298/BAH1204873S
Stanišić N, Aleksić S, Di L, Stanimirović Z, Zhenhua G, Petrović M, Delić N, Radović Č, Parunović N, Gogić M. Porcine stress syndrome (PSS) in Mangalitsa pigs. in Biotechnology in Animal Husbandry. 2012;28(4):873-878.
doi:10.2298/BAH1204873S .
Stanišić, Nikola, Aleksić, S., Di, L., Stanimirović, Zoran, Zhenhua, G., Petrović, M., Delić, Nikola, Radović, Čedomir, Parunović, Nenad, Gogić, Marija, "Porcine stress syndrome (PSS) in Mangalitsa pigs" in Biotechnology in Animal Husbandry, 28, no. 4 (2012):873-878,
https://doi.org/10.2298/BAH1204873S . .
