Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root
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2012
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The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed t...hat the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria. (C) 2012 Elsevier Ltd. All rights reserved.
Ključne reči:
Pyrenochaeta terrestris / Identification / Quantification / Method / Maize rootIzvor:
Crop Protection, 2012, 38, 66-71Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Poboljšanje svojstava kukuruza i soje molekularnim i konvencionalnim oplemenjivanjem (RS-MESTD-Technological Development (TD or TR)-31068)
- Redukcija toksigenih gljiva roda Fusarium i njihovih mikotoksina u proizvodnji zdravstveno bezbedne hrane na bazi žita (RS-MESTD-Technological Development (TD or TR)-31023)
DOI: 10.1016/j.cropro.2012.03.015
ISSN: 0261-2194
WoS: 000305921400011
Scopus: 2-s2.0-84861008610
Institucija/grupa
RIStocarTY - JOUR AU - Lević, Jelena AU - Stanković, Slavica Ž. AU - Krnjaja, Vesna AU - Bočarov-Stančić, Aleksandra S. PY - 2012 UR - http://r.istocar.bg.ac.rs/handle/123456789/315 AB - The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria. (C) 2012 Elsevier Ltd. All rights reserved. PB - Elsevier Sci Ltd, Oxford T2 - Crop Protection T1 - Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root VL - 38 SP - 66 EP - 71 DO - 10.1016/j.cropro.2012.03.015 ER -
@article{ author = "Lević, Jelena and Stanković, Slavica Ž. and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra S.", year = "2012", abstract = "The present study describes the developed filter layer method for the consistent determination and identification of the natural occurrence of Pyrenochaeta terrestris in maize roots. The field experiment was set up to establish the applicability, accuracy and efficiency of this method for the pathogen isolation from roots of maize grown under different agroecological conditions. According to this method, the 10- to 15-mm long tissue sections, mainly cut at the margin of lesions developed in roots, were placed on two layers of moisten filter paper in Petri dishes and incubated for 6-7 days under laboratory conditions, 25 +/- 2 degrees C and daylight. The unique reddish-purple (beet-red) pigmentation was used as an indicator of the fungal incidence in infected roots, which facilitated a succeeding confirmation of the pathogen on PDA at 25 degrees C in the dark and on CLA in the alternating 12 h light (fluorescent and near-ultraviolet light)/12 h dark conditions. Obtained results showed that the filter layer method was very effective for the P. terrestris detection and that it could be applied for a rapid assessment of response of maize genotypes, environmental and seasonal effects on the fungal growth on roots and the fungal distribution. Advantages of this method for the detection and quantification of P. terrestris in relation to the methods described in the literature include: a) low investments, because many root samples can be tested immediately at the same time; b) results can be obtained rapidly (6-7 days after sampling); and c) minimal interference by other fungi or bacteria. (C) 2012 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Crop Protection", title = "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root", volume = "38", pages = "66-71", doi = "10.1016/j.cropro.2012.03.015" }
Lević, J., Stanković, S. Ž., Krnjaja, V.,& Bočarov-Stančić, A. S.. (2012). Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection Elsevier Sci Ltd, Oxford., 38, 66-71. https://doi.org/10.1016/j.cropro.2012.03.015
Lević J, Stanković SŽ, Krnjaja V, Bočarov-Stančić AS. Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root. in Crop Protection. 2012;38:66-71. doi:10.1016/j.cropro.2012.03.015 .
Lević, Jelena, Stanković, Slavica Ž., Krnjaja, Vesna, Bočarov-Stančić, Aleksandra S., "Simple and efficient method for detection and identification of Pyrenochaeta terrestris on maize root" in Crop Protection, 38 (2012):66-71, https://doi.org/10.1016/j.cropro.2012.03.015 . .