TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/516
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Zorić, Andrea
AU  - Bojkovski, Jovan
AU  - Stanojković, Aleksandar
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/487
AB  - Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods.
AB  - Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - The application of PCR based methods in diagnostics of some viral infections of swine
T1  - Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja
VL  - 32
IS  - 4
SP  - 321
EP  - 329
DO  - 10.2298/bah1604321N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Zorić, Andrea and Bojkovski, Jovan and Stanojković, Aleksandar",
year = "2016",
abstract = "Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods., Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "The application of PCR based methods in diagnostics of some viral infections of swine, Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja",
volume = "32",
number = "4",
pages = "321-329",
doi = "10.2298/bah1604321N"
}

Nišavić, J., Milić, N., Zorić, A., Bojkovski, J.,& Stanojković, A.. (2016). The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(4), 321-329.
https://doi.org/10.2298/bah1604321N

Nišavić J, Milić N, Zorić A, Bojkovski J, Stanojković A. The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry. 2016;32(4):321-329.
doi:10.2298/bah1604321N .

Nišavić, Jakov, Milić, Nenad, Zorić, Andrea, Bojkovski, Jovan, Stanojković, Aleksandar, "The application of PCR based methods in diagnostics of some viral infections of swine" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):321-329,
https://doi.org/10.2298/bah1604321N . .