TY  - JOUR
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra S.
AU  - Tančić, Sonja L.
AU  - Kovačević, Tamara M.
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/138
AB  - A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively.
AB  - Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia
T1  - Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji
IS  - 113
SP  - 93
EP  - 102
DO  - 10.2298/ZMSPN0713093S
ER  - 

@article{
author = "Stanković, Slavica Ž. and Lević, Jelena and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra S. and Tančić, Sonja L. and Kovačević, Tamara M.",
year = "2007",
abstract = "A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively., Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia, Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji",
number = "113",
pages = "93-102",
doi = "10.2298/ZMSPN0713093S"
}

Stanković, S. Ž., Lević, J., Krnjaja, V., Bočarov-Stančić, A. S., Tančić, S. L.,& Kovačević, T. M.. (2007). Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 93-102.
https://doi.org/10.2298/ZMSPN0713093S

Stanković SŽ, Lević J, Krnjaja V, Bočarov-Stančić AS, Tančić SL, Kovačević TM. Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke. 2007;(113):93-102.
doi:10.2298/ZMSPN0713093S .

Stanković, Slavica Ž., Lević, Jelena, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra S., Tančić, Sonja L., Kovačević, Tamara M., "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):93-102,
https://doi.org/10.2298/ZMSPN0713093S . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Tomić, Zorica
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/139
AB  - Pathogenicity of 10 Fusarium verticillioides isolates, originated from grain of wheat (five isolates) and maize (five isolates), were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate (a scale for % of nonemerged plants, % of survived plants, plant vigour - the growth and dry weight of roots and epicotyls and disease severity) it was determined that all F. verticillioides isolates expressed a different degree of pathogenicity. According to % of nonemerged plants six three and one F. verticillioides isolates expressed low, moderate and high degree of pathogenicity, respectively. All F. verticillioides isolates reduced the plant survival rate and vigour, while the disease severity ranged from 2.0 to 3.54. Two types of nit mutants, nit1 and NitM, were obtained by the use of the method of vegetative compatibility. The frequency of nit1 mutants was greater (58.79%) than the frequency of NitM mutants (5.77%). A total of 10 vegetative compatibility groups (VCGs) of F. verticillioides were established in the complementation tests. These results point out to a high genetic diversity of F. verticillioides population.
AB  - Patogenost 10 izolata F. verticillioides, poreklom iz zrna pšenice (5 izolata) i kukuruza (5 izolata), proučavana je u uslovima staklenika. Na osnovu različitih parametara ocene patogenosti (skala za % neizniklih biljaka, % preživelih biljaka, vigora biljaka - porast i suva težina korena i epikotila, i intenziteta bolesti) ustanovljeno je da su svi ispitivani izolati F. verticillioides ispoljili različit stepen patogenosti. Prema % neizniklih biljaka 6 izolata ispoljilo je nisku, 3 izolata srednju i jedan izolat F. verticillioides visoku patogenost. Svi ispitivani izolati F. verticillioides su prouzrokovali smanjeno preživljavanje i vigor biljaka, sa intenzitetom bolesti od 2.0 do 3.54. Primenom metode vegetativne kompatibilnosti izolovane su dve vrste nit mutanata, nit1 i NitM. Učestalost mutanata nit1 je bila veća (58.79%) u odnosu na NitM mutante (5.77%). U komplementarnim testovima ustanovljeno je 10 vegetativno kompatibilnih grupa (VCG) F. verticillioides što ukazuje na visok genetički diverzitet populacije ovog patogena.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides
T1  - Patogenost i diverzitet vegetativne kompatibilnosti Fusarium verticillioides
IS  - 113
SP  - 103
EP  - 111
DO  - 10.2298/ZMSPN0713103K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Tomić, Zorica",
year = "2007",
abstract = "Pathogenicity of 10 Fusarium verticillioides isolates, originated from grain of wheat (five isolates) and maize (five isolates), were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate (a scale for % of nonemerged plants, % of survived plants, plant vigour - the growth and dry weight of roots and epicotyls and disease severity) it was determined that all F. verticillioides isolates expressed a different degree of pathogenicity. According to % of nonemerged plants six three and one F. verticillioides isolates expressed low, moderate and high degree of pathogenicity, respectively. All F. verticillioides isolates reduced the plant survival rate and vigour, while the disease severity ranged from 2.0 to 3.54. Two types of nit mutants, nit1 and NitM, were obtained by the use of the method of vegetative compatibility. The frequency of nit1 mutants was greater (58.79%) than the frequency of NitM mutants (5.77%). A total of 10 vegetative compatibility groups (VCGs) of F. verticillioides were established in the complementation tests. These results point out to a high genetic diversity of F. verticillioides population., Patogenost 10 izolata F. verticillioides, poreklom iz zrna pšenice (5 izolata) i kukuruza (5 izolata), proučavana je u uslovima staklenika. Na osnovu različitih parametara ocene patogenosti (skala za % neizniklih biljaka, % preživelih biljaka, vigora biljaka - porast i suva težina korena i epikotila, i intenziteta bolesti) ustanovljeno je da su svi ispitivani izolati F. verticillioides ispoljili različit stepen patogenosti. Prema % neizniklih biljaka 6 izolata ispoljilo je nisku, 3 izolata srednju i jedan izolat F. verticillioides visoku patogenost. Svi ispitivani izolati F. verticillioides su prouzrokovali smanjeno preživljavanje i vigor biljaka, sa intenzitetom bolesti od 2.0 do 3.54. Primenom metode vegetativne kompatibilnosti izolovane su dve vrste nit mutanata, nit1 i NitM. Učestalost mutanata nit1 je bila veća (58.79%) u odnosu na NitM mutante (5.77%). U komplementarnim testovima ustanovljeno je 10 vegetativno kompatibilnih grupa (VCG) F. verticillioides što ukazuje na visok genetički diverzitet populacije ovog patogena.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides, Patogenost i diverzitet vegetativne kompatibilnosti Fusarium verticillioides",
number = "113",
pages = "103-111",
doi = "10.2298/ZMSPN0713103K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž.,& Tomić, Z.. (2007). Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 103-111.
https://doi.org/10.2298/ZMSPN0713103K

Krnjaja V, Lević J, Stanković SŽ, Tomić Z. Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides. in Zbornik Matice srpske za prirodne nauke. 2007;(113):103-111.
doi:10.2298/ZMSPN0713103K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Tomić, Zorica, "Pathogenicity and diversity of vegetative compatibility of Fusarium verticillioides" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):103-111,
https://doi.org/10.2298/ZMSPN0713103K . .

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra S.
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara M.
AU  - Tančić, Sonja L.
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/141
AB  - Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively.
AB  - U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The toxigenic potential of Fusarium poae originated from wheat
T1  - Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice
IS  - 113
SP  - 113
EP  - 123
DO  - 10.2298/ZMSPN0713113B
ER  - 

@article{
author = "Bočarov-Stančić, Aleksandra S. and Lević, Jelena and Stanković, Slavica Ž. and Krnjaja, Vesna and Kovačević, Tamara M. and Tančić, Sonja L.",
year = "2007",
abstract = "Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively., U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The toxigenic potential of Fusarium poae originated from wheat, Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice",
number = "113",
pages = "113-123",
doi = "10.2298/ZMSPN0713113B"
}

Bočarov-Stančić, A. S., Lević, J., Stanković, S. Ž., Krnjaja, V., Kovačević, T. M.,& Tančić, S. L.. (2007). The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 113-123.
https://doi.org/10.2298/ZMSPN0713113B

Bočarov-Stančić AS, Lević J, Stanković SŽ, Krnjaja V, Kovačević TM, Tančić SL. The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke. 2007;(113):113-123.
doi:10.2298/ZMSPN0713113B .

Bočarov-Stančić, Aleksandra S., Lević, Jelena, Stanković, Slavica Ž., Krnjaja, Vesna, Kovačević, Tamara M., Tančić, Sonja L., "The toxigenic potential of Fusarium poae originated from wheat" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):113-123,
https://doi.org/10.2298/ZMSPN0713113B . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Tomić, Zorica
AU  - Nešić, Zorica
AU  - Stojanović, Ljiljana
AU  - Trenkovski, Snežana
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/142
AB  - Production and providing of high quality maize grain are of primary importance for livestock production since maize is the main component of livestock feed. Contamination with fungi not only diminishes the quality of grain, but some fungi species can produce highly toxic compounds known as mycotoxins. Considering that maize is economically the most important grown plant in Serbia, content and intensity of frequency of these pathogen fungi species are investigated in maize stored in the storage facilities. Based on studies of the fungi incidence in stored maize intended for nutrition of livestock, it was established that during the period from February 2005 to March 2006, some fungi species were present successively (Fusarium spp., Penicillium spp.), other with interruptions of one (Nigrospora spp., Aspergillus spp., Acremonium spp., Alternaria spp), three (Mucor spp., Chaetomium spp., Rhizopus spp.) to four months (Cladosporium spp.), and also sporadically during three (Epicoccum spp., Rhizopus spp., Trichothecium spp.), two (Gliocladium spp.) or one month (Papulaspora spp., Phialophora spp.). Period June-October is the most critical for preservation of the quality of stored maize since the number of fungi species present (8−14 of total of 17 identified fungi genera) and their frequency during this period are the highest, especially of toxigenic species of Fusarium (43.5−62.5%) and Penicillium (10.0−33.5%) genera. Positive correlation (r = 0.5979**) between dynamics of incidence and frequency of isolated fungi species indicates that fungi with higher frequency of incidence remain longer on the grain during storage. F. verticillioides and F. subglutinans, of total of 6 identified Fusarium species were present on the grain during entire year and with the highest frequency (24.7% and 5.9%, respectively). During single year (2005) F. verticillioides was present in the highest percentage from February to October (22.0−39.5%), and F. subglutinans from April to October (8.0−12.5%), whereas the both species were isolated the least during winter period December-January (4.0−8.0% and 0.5−1.0%, respectively). F. graminearaum is the third toxigenic Fusarium species which from the mycotoxicological aspect can be important for period June-September when it is mostly present (5.0−11.0%). Incidence of other Fusarium species is sporadic (1.3% F. proliferatum, 1.0% F. sambucinum and 0.5% F. poae).
AB  - Proizvodnja i obezbeđivanje visokog kvaliteta zrna kukuruza su od primarnog značaja za stočarsku proizvodnju jer je kukuruz osnovna komponenta stočne hrane. Zaraza gljivama ne samo da smanjuje kvalitet zrna, već neke vrste gljiva mogu produkovati visoko toksična jedinjenja, poznata kao mikotoksini. S obzirom da je kukuruz ekonomski najvažnija gajena biljka u Srbiji, sadržaj i intenzitet učestalosti ovih patogenih vrsta gljiva se ispituje u kukuruzu u skladištu. Na osnovu proučavanja pojave gljiva na uskladištenom kukuruzu, koji je bio namenjen za ishranu životinja, utvrđeno je da su u periodu od februara 2005. do marta 2006. godine neke gljive prisutne sukcesivno (Fusarium spp., Penicillium spp.), druge diskontinuirano sa prekidom od jednog (Nigrospora spp., Aspergillus spp., Acremonium spp., Alternaria spp), tri (Mucor spp., Chaetomium spp., Rhizopus spp.) ili četiri meseca (Cladosporium spp.), a treće sporadično u toku tri (Epicoccum spp., Rhizopus spp., Trichothecium spp.), dva (Gliocladium spp.) ili jednog meseca (Papulaspora spp., Phialophora spp.). Period jun-oktobar je najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer je brojnost (prisutno 8−14 od ukupno 17 identifikovanih rodova gljiva) i frekvencija gljiva u tom periodu najveća, posebno toksigenih vrsta iz rodova Fusarium (43.5−62.5%) i Penicillium (10.0−33.5%). Pozitivna korelacija (r = 0.5979**) između dinamike pojave i frekvencije izolovanih vrsta gljiva ukazuje da se gljive sa većom frekvencijom pojave duže održavaju na zrnu kukuruza tokom skladištenja. F. verticillioides i F. subglutinans su od ukupno šest identifikovanih vrsta roda Fusarium prisutne na zrnu tokom cele godine i sa najvećom frekvencijom (24.7% i 5.9%). U toku jedne godine (2005) F. verticillioides je u najvećem procentu prisutna od februara do oktobra (22.0−39.5%), a F. subglutinans od aprila do oktobra (8.0−12.5%), dok su obe vrste najmanje izolovane u zimskom periodu decembar-januar (4.0−8.0% i 0.5−1.0%). F. graminearum je treća toksigena vrsta roda Fusarium koja sa mikotoksikološkog aspekta može biti značajna za period jun-septembar kada se javlja u najvećem procentu (5.0−11.0%). Pojava ostalih vrsta roda Fusarium je sporadična (1.3% F. proliferatum, 1.0% F. sambucinum and 0.5% F. poae).
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain
T1  - Dinamika pojave i učestalosti populacija Fusarium vrsta na uskladištenom zrnu kukuruza
VL  - 23
IS  - 5-6-1
SP  - 589
EP  - 600
DO  - 10.2298/BAH0701589K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Tomić, Zorica and Nešić, Zorica and Stojanović, Ljiljana and Trenkovski, Snežana",
year = "2007",
abstract = "Production and providing of high quality maize grain are of primary importance for livestock production since maize is the main component of livestock feed. Contamination with fungi not only diminishes the quality of grain, but some fungi species can produce highly toxic compounds known as mycotoxins. Considering that maize is economically the most important grown plant in Serbia, content and intensity of frequency of these pathogen fungi species are investigated in maize stored in the storage facilities. Based on studies of the fungi incidence in stored maize intended for nutrition of livestock, it was established that during the period from February 2005 to March 2006, some fungi species were present successively (Fusarium spp., Penicillium spp.), other with interruptions of one (Nigrospora spp., Aspergillus spp., Acremonium spp., Alternaria spp), three (Mucor spp., Chaetomium spp., Rhizopus spp.) to four months (Cladosporium spp.), and also sporadically during three (Epicoccum spp., Rhizopus spp., Trichothecium spp.), two (Gliocladium spp.) or one month (Papulaspora spp., Phialophora spp.). Period June-October is the most critical for preservation of the quality of stored maize since the number of fungi species present (8−14 of total of 17 identified fungi genera) and their frequency during this period are the highest, especially of toxigenic species of Fusarium (43.5−62.5%) and Penicillium (10.0−33.5%) genera. Positive correlation (r = 0.5979**) between dynamics of incidence and frequency of isolated fungi species indicates that fungi with higher frequency of incidence remain longer on the grain during storage. F. verticillioides and F. subglutinans, of total of 6 identified Fusarium species were present on the grain during entire year and with the highest frequency (24.7% and 5.9%, respectively). During single year (2005) F. verticillioides was present in the highest percentage from February to October (22.0−39.5%), and F. subglutinans from April to October (8.0−12.5%), whereas the both species were isolated the least during winter period December-January (4.0−8.0% and 0.5−1.0%, respectively). F. graminearaum is the third toxigenic Fusarium species which from the mycotoxicological aspect can be important for period June-September when it is mostly present (5.0−11.0%). Incidence of other Fusarium species is sporadic (1.3% F. proliferatum, 1.0% F. sambucinum and 0.5% F. poae)., Proizvodnja i obezbeđivanje visokog kvaliteta zrna kukuruza su od primarnog značaja za stočarsku proizvodnju jer je kukuruz osnovna komponenta stočne hrane. Zaraza gljivama ne samo da smanjuje kvalitet zrna, već neke vrste gljiva mogu produkovati visoko toksična jedinjenja, poznata kao mikotoksini. S obzirom da je kukuruz ekonomski najvažnija gajena biljka u Srbiji, sadržaj i intenzitet učestalosti ovih patogenih vrsta gljiva se ispituje u kukuruzu u skladištu. Na osnovu proučavanja pojave gljiva na uskladištenom kukuruzu, koji je bio namenjen za ishranu životinja, utvrđeno je da su u periodu od februara 2005. do marta 2006. godine neke gljive prisutne sukcesivno (Fusarium spp., Penicillium spp.), druge diskontinuirano sa prekidom od jednog (Nigrospora spp., Aspergillus spp., Acremonium spp., Alternaria spp), tri (Mucor spp., Chaetomium spp., Rhizopus spp.) ili četiri meseca (Cladosporium spp.), a treće sporadično u toku tri (Epicoccum spp., Rhizopus spp., Trichothecium spp.), dva (Gliocladium spp.) ili jednog meseca (Papulaspora spp., Phialophora spp.). Period jun-oktobar je najkritičniji za očuvanje kvaliteta uskladištenog kukuruza jer je brojnost (prisutno 8−14 od ukupno 17 identifikovanih rodova gljiva) i frekvencija gljiva u tom periodu najveća, posebno toksigenih vrsta iz rodova Fusarium (43.5−62.5%) i Penicillium (10.0−33.5%). Pozitivna korelacija (r = 0.5979**) između dinamike pojave i frekvencije izolovanih vrsta gljiva ukazuje da se gljive sa većom frekvencijom pojave duže održavaju na zrnu kukuruza tokom skladištenja. F. verticillioides i F. subglutinans su od ukupno šest identifikovanih vrsta roda Fusarium prisutne na zrnu tokom cele godine i sa najvećom frekvencijom (24.7% i 5.9%). U toku jedne godine (2005) F. verticillioides je u najvećem procentu prisutna od februara do oktobra (22.0−39.5%), a F. subglutinans od aprila do oktobra (8.0−12.5%), dok su obe vrste najmanje izolovane u zimskom periodu decembar-januar (4.0−8.0% i 0.5−1.0%). F. graminearum je treća toksigena vrsta roda Fusarium koja sa mikotoksikološkog aspekta može biti značajna za period jun-septembar kada se javlja u najvećem procentu (5.0−11.0%). Pojava ostalih vrsta roda Fusarium je sporadična (1.3% F. proliferatum, 1.0% F. sambucinum and 0.5% F. poae).",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain, Dinamika pojave i učestalosti populacija Fusarium vrsta na uskladištenom zrnu kukuruza",
volume = "23",
number = "5-6-1",
pages = "589-600",
doi = "10.2298/BAH0701589K"
}

Krnjaja, V., Lević, J., Tomić, Z., Nešić, Z., Stojanović, L.,& Trenkovski, S.. (2007). Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 23(5-6-1), 589-600.
https://doi.org/10.2298/BAH0701589K

Krnjaja V, Lević J, Tomić Z, Nešić Z, Stojanović L, Trenkovski S. Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain. in Biotechnology in Animal Husbandry. 2007;23(5-6-1):589-600.
doi:10.2298/BAH0701589K .

Krnjaja, Vesna, Lević, Jelena, Tomić, Zorica, Nešić, Zorica, Stojanović, Ljiljana, Trenkovski, Snežana, "Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain" in Biotechnology in Animal Husbandry, 23, no. 5-6-1 (2007):589-600,
https://doi.org/10.2298/BAH0701589K . .