TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Miličić, Danijel
AU  - Petrović, Tanja
AU  - Krnjaja, Vesna
AU  - Stanojević, Slađana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/609
AB  - Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a w values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.
PB  - MDPI, Basel, Switzerland
T2  - Toxins
T1  - Mycotoxins and mycotoxin producing fungi in pollen: Review
VL  - 11
IS  - 2
SP  - 64
DO  - 10.3390/toxins11020064
ER  - 

@article{
author = "Kostić, Aleksandar and Miličić, Danijel and Petrović, Tanja and Krnjaja, Vesna and Stanojević, Slađana and Barać, Miroljub and Tešić, Živoslav and Pešić, Mirjana",
year = "2019",
abstract = "Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a w values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.",
publisher = "MDPI, Basel, Switzerland",
journal = "Toxins",
title = "Mycotoxins and mycotoxin producing fungi in pollen: Review",
volume = "11",
number = "2",
pages = "64",
doi = "10.3390/toxins11020064"
}

Kostić, A., Miličić, D., Petrović, T., Krnjaja, V., Stanojević, S., Barać, M., Tešić, Ž.,& Pešić, M.. (2019). Mycotoxins and mycotoxin producing fungi in pollen: Review. in Toxins
MDPI, Basel, Switzerland., 11(2), 64.
https://doi.org/10.3390/toxins11020064

Kostić A, Miličić D, Petrović T, Krnjaja V, Stanojević S, Barać M, Tešić Ž, Pešić M. Mycotoxins and mycotoxin producing fungi in pollen: Review. in Toxins. 2019;11(2):64.
doi:10.3390/toxins11020064 .

Kostić, Aleksandar, Miličić, Danijel, Petrović, Tanja, Krnjaja, Vesna, Stanojević, Slađana, Barać, Miroljub, Tešić, Živoslav, Pešić, Mirjana, "Mycotoxins and mycotoxin producing fungi in pollen: Review" in Toxins, 11, no. 2 (2019):64,
https://doi.org/10.3390/toxins11020064 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://istocar.bg.ac.rs/wp-content/uploads/2019/03/06.BAH-19-1-10-26.2.2019.-Krnjaja-et-al-1-1.pdf
UR  - http://r.istocar.bg.ac.rs/handle/123456789/610
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method.
The total fungal count was in the range from 1 x 102 (2 log CFU g–1) to 1.83 x 105 CFU g–1 (5.26 log CFU g–1). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g–1, whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g–1. In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 μg kg–1, with an average of 4.47 and 4.56 μg kg–1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits.
The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz razliĉitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razređenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA).
Ukupan broj gljiva bio je od 1 x 102 (2 log CFU g–1) do 1,83 x 105 CFU g–1 (5.26 log CFU g–1). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g–1, dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g–1. U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 μg kg–1, sa proseĉnim sadržajem od 4,47 μg kg–1 u uzorcima hrane za piliće, i 4,56 μg kg–1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju znaĉaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljiviĉnih) kontaminenata u lancu ishrane.
PB  - Belgrade : Institute for animal husbandry
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 

@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method.
The total fungal count was in the range from 1 x 102 (2 log CFU g–1) to 1.83 x 105 CFU g–1 (5.26 log CFU g–1). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g–1, whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g–1. In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 μg kg–1, with an average of 4.47 and 4.56 μg kg–1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits.
The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz razliĉitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razređenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA).
Ukupan broj gljiva bio je od 1 x 102 (2 log CFU g–1) do 1,83 x 105 CFU g–1 (5.26 log CFU g–1). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g–1, dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g–1. U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 μg kg–1, sa proseĉnim sadržajem od 4,47 μg kg–1 u uzorcima hrane za piliće, i 4,56 μg kg–1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju znaĉaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljiviĉnih) kontaminenata u lancu ishrane.",
publisher = "Belgrade : Institute for animal husbandry",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}

Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z.. (2019). Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry
Belgrade : Institute for animal husbandry., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K

Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry. 2019;35(1):61-69.
doi:10.2298/BAH1901061K .

Krnjaja, Vesna, Petrović, Tanja, Stanković, Slavica, Lukić, Miloš, Škrbić, Zdenka, Mandić, Violeta, Bijelić, Zorica, "Mycobiota and aflatoxin B1 in poultry feeds" in Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Jauković, Marko
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/626
AB  - Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
T1  - The effect of climate conditions on aflatoxin contamination of cereal grains and feeds
SP  - 580
EP  - 591
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_626
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Jauković, Marko",
year = "2019",
abstract = "Production and consumption of healthy food occur as the
necessity of the modern life habits. The reduced risk of diseases improves the
quality of life. Feed safety is a priority trend in all process of animal production.
However, toxigenic fungal species and their secondary metabolites (mycotoxins)
are the common feed contaminants. The aflatoxins and the producing Aspergillus
species section Flavi in cereal grains and feeds has emerged as a serious concern
with potential health hazards in humans and animals. This paper aimed to give an
overview on of the effect of climatic factors on Aspergillus and aflatoxin
contamination of cereals and feeds in agro-ecological conditions of Serbia and
other European countries. Since the reduction of health risks and diseases in the
livestock as consequences of aflatoxins presence in feed have become the priority
tasks in feed safety control strategy thus the aim of this review has also been to
recommend some of the measures for preventing their adverse effect.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia",
title = "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds",
pages = "580-591",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_626"
}

Krnjaja, V., Stanković, S., Obradović, A., Petrović, T., Mandić, V., Bijelić, Z.,& Jauković, M.. (2019). The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia
Institute for Animal Husbandry, Belgrade-Zemun., 580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626

Krnjaja V, Stanković S, Obradović A, Petrović T, Mandić V, Bijelić Z, Jauković M. The effect of climate conditions on aflatoxin contamination of cereal grains and feeds. in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia. 2019;:580-591.
https://hdl.handle.net/21.15107/rcub_ristocar_626 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Jauković, Marko, "The effect of climate conditions on aflatoxin contamination of cereal grains and feeds" in Proceedings, 12th International Symposium "Modern Trends in Livestock Production", 9-11 October 2019, Belgrade, Serbia (2019):580-591,
https://hdl.handle.net/21.15107/rcub_ristocar_626 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica Ž.
AU  - Obradović, A.
AU  - Petrović, Tanja S.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Božić, M.
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/550
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg-1) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - MDPI AG
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica Ž. and Obradović, A. and Petrović, Tanja S. and Mandić, Violeta and Bijelić, Zorica and Božić, M.",
year = "2018",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg-1) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "MDPI AG",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}

Krnjaja, V., Stanković, S. Ž., Obradović, A., Petrović, T. S., Mandić, V., Bijelić, Z.,& Božić, M.. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. in Toxins
MDPI AG., 10(11).
https://doi.org/10.3390/toxins10110460

Krnjaja V, Stanković SŽ, Obradović A, Petrović TS, Mandić V, Bijelić Z, Božić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. in Toxins. 2018;10(11).
doi:10.3390/toxins10110460 .

Krnjaja, Vesna, Stanković, Slavica Ž., Obradović, A., Petrović, Tanja S., Mandić, Violeta, Bijelić, Zorica, Božić, M., "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" in Toxins, 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica Ž.
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja S.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/549
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica Ž. and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja S. and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}

Krnjaja, V., Stanković, S. Ž., Lukić, M., Mićić, N., Petrović, T. S., Bijelić, Z.,& Mandić, V.. (2018). Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K

Krnjaja V, Stanković SŽ, Lukić M, Mićić N, Petrović TS, Bijelić Z, Mandić V. Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia. in Biotechnology in Animal Husbandry. 2018;34(2):239-249.
doi:10.2298/bah1802239K .

Krnjaja, Vesna, Stanković, Slavica Ž., Lukić, Miloš, Mićić, Nenad, Petrović, Tanja S., Bijelić, Zorica, Mandić, Violeta, "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia" in Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K . .

TY  - JOUR
AU  - Kostic, Aleksandar Z.
AU  - Petrović, Tanja S.
AU  - Krnjaja, Vesna
AU  - Nedic, Nebojša M.
AU  - Tešić, Živoslav Lj.
AU  - Milojkovic-Opsenica, Dusanka M.
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladana P.
AU  - Pesic, Mirjana B.
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/519
AB  - Assessment of microbiological quality of bee collected pollen is very important, because of its use as a supplement in the human diet. In this study, 26 samples collected from different location in Serbia were tested for the presence of mold through mycologial analysis. The presence of aflatoxin B1, one of the most dangerous and the most widespread mycotoxin was also determined. It was established that 10 of the investigated samples were contaminated with some genera or species of mold, but all of the investigated samples were contaminated with aflatoxin B1. Considering that there is no unique and official procedure for mycological analysis of bee collected pollen, these findings suggest the need for their establishment. Mycological analysis should be followed by mycotoxicological analysis since the absence of mold does not confirm the absence of aflatoxin B1 in bee pollen.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions
VL  - 56
IS  - 1
SP  - 13
EP  - 20
DO  - 10.1080/00218839.2016.1259897
ER  - 

@article{
author = "Kostic, Aleksandar Z. and Petrović, Tanja S. and Krnjaja, Vesna and Nedic, Nebojša M. and Tešić, Živoslav Lj. and Milojkovic-Opsenica, Dusanka M. and Barac, Miroljub B. and Stanojević, Sladana P. and Pesic, Mirjana B.",
year = "2017",
abstract = "Assessment of microbiological quality of bee collected pollen is very important, because of its use as a supplement in the human diet. In this study, 26 samples collected from different location in Serbia were tested for the presence of mold through mycologial analysis. The presence of aflatoxin B1, one of the most dangerous and the most widespread mycotoxin was also determined. It was established that 10 of the investigated samples were contaminated with some genera or species of mold, but all of the investigated samples were contaminated with aflatoxin B1. Considering that there is no unique and official procedure for mycological analysis of bee collected pollen, these findings suggest the need for their establishment. Mycological analysis should be followed by mycotoxicological analysis since the absence of mold does not confirm the absence of aflatoxin B1 in bee pollen.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions",
volume = "56",
number = "1",
pages = "13-20",
doi = "10.1080/00218839.2016.1259897"
}

Kostic, A. Z., Petrović, T. S., Krnjaja, V., Nedic, N. M., Tešić, Ž. Lj., Milojkovic-Opsenica, D. M., Barac, M. B., Stanojević, S. P.,& Pesic, M. B.. (2017). Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 56(1), 13-20.
https://doi.org/10.1080/00218839.2016.1259897

Kostic AZ, Petrović TS, Krnjaja V, Nedic NM, Tešić ŽL, Milojkovic-Opsenica DM, Barac MB, Stanojević SP, Pesic MB. Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions. in Journal of Apicultural Research. 2017;56(1):13-20.
doi:10.1080/00218839.2016.1259897 .

Kostic, Aleksandar Z., Petrović, Tanja S., Krnjaja, Vesna, Nedic, Nebojša M., Tešić, Živoslav Lj., Milojkovic-Opsenica, Dusanka M., Barac, Miroljub B., Stanojević, Sladana P., Pesic, Mirjana B., "Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions" in Journal of Apicultural Research, 56, no. 1 (2017):13-20,
https://doi.org/10.1080/00218839.2016.1259897 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Zorica
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, A.
PY  - 2015
UR  - http://r.istocar.bg.ac.rs/handle/123456789/472
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 

@article{
author = "Krnjaja, Vesna and Tomić, Zorica and Stanković, Slavica Ž. and Petrović, Tanja S. and Bijelić, Zorica and Mandić, Violeta and Obradović, A.",
year = "2015",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}

Krnjaja, V., Tomić, Z., Stanković, S. Ž., Petrović, T. S., Bijelić, Z., Mandić, V.,& Obradović, A.. (2015). Fusarium infection and deoxynivalenol contamination in winter wheat. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K

Krnjaja V, Tomić Z, Stanković SŽ, Petrović TS, Bijelić Z, Mandić V, Obradović A. Fusarium infection and deoxynivalenol contamination in winter wheat. in Biotechnology in Animal Husbandry. 2015;31(1):123-131.
doi:10.2298/BAH1501123K .

Krnjaja, Vesna, Tomić, Zorica, Stanković, Slavica Ž., Petrović, Tanja S., Bijelić, Zorica, Mandić, Violeta, Obradović, A., "Fusarium infection and deoxynivalenol contamination in winter wheat" in Biotechnology in Animal Husbandry, 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Mandić, Violeta
AU  - Tomić, Zorica
AU  - Obradović, A.
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/403
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Mandić, Violeta and Tomić, Zorica and Obradović, A.",
year = "2014",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Mandić, V., Tomić, Z.,& Obradović, A.. (2014). Presence of deoxynivalenol in winter wheat treated with fungicides. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K

Krnjaja V, Lević J, Stanković SŽ, Petrović TS, Mandić V, Tomić Z, Obradović A. Presence of deoxynivalenol in winter wheat treated with fungicides. in Biotechnology in Animal Husbandry. 2014;30(1):167-173.
doi:10.2298/BAH1401167K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Mandić, Violeta, Tomić, Zorica, Obradović, A., "Presence of deoxynivalenol in winter wheat treated with fungicides" in Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K . .

TY  - JOUR
AU  - Stajić, Slaviša
AU  - Živković, Dušan
AU  - Tomović, Vladimir
AU  - Nedović, Viktor
AU  - Perunović, Marija
AU  - Kovjanić, Nataša
AU  - Lević, Steva
AU  - Stanišić, Nikola
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/430
AB  - This study aims to examine the scope of changes in colour, texture and sensory characteristics of fermented sausages by substituting 20% of backfat with grapeseed oil prepared as liquid (GS), encapsulated (EGS) and pre-emulsified [with soy protein isolate (GSI) and alginate (GSA)], and their stability during 30days of refrigerated storage. Protein and fat contents, pH and TBA values were not significantly different between products after production and storage. Treatments with added oil had higher weight loss (1.5-2.5%), while moisture content was significantly lowest in GS. Internal product colour evaluation showed the highest values of L*, b* and hue angle in GSI. GSI had the highest hardness and chewiness. Storage affected more surface than internal product colour, hardness was significantly changed only in GSI (lower) and EGS (higher), and increasing of TBA did not lead to significant differences between treatments. The study showed that GSA was the most promising of all treatments investigated.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Food Science and Technology
T1  - The utilisation of grapeseed oil in improving the quality of dry fermented sausages
VL  - 49
IS  - 11
SP  - 2356
EP  - 2363
DO  - 10.1111/ijfs.12555
ER  - 

@article{
author = "Stajić, Slaviša and Živković, Dušan and Tomović, Vladimir and Nedović, Viktor and Perunović, Marija and Kovjanić, Nataša and Lević, Steva and Stanišić, Nikola",
year = "2014",
abstract = "This study aims to examine the scope of changes in colour, texture and sensory characteristics of fermented sausages by substituting 20% of backfat with grapeseed oil prepared as liquid (GS), encapsulated (EGS) and pre-emulsified [with soy protein isolate (GSI) and alginate (GSA)], and their stability during 30days of refrigerated storage. Protein and fat contents, pH and TBA values were not significantly different between products after production and storage. Treatments with added oil had higher weight loss (1.5-2.5%), while moisture content was significantly lowest in GS. Internal product colour evaluation showed the highest values of L*, b* and hue angle in GSI. GSI had the highest hardness and chewiness. Storage affected more surface than internal product colour, hardness was significantly changed only in GSI (lower) and EGS (higher), and increasing of TBA did not lead to significant differences between treatments. The study showed that GSA was the most promising of all treatments investigated.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "The utilisation of grapeseed oil in improving the quality of dry fermented sausages",
volume = "49",
number = "11",
pages = "2356-2363",
doi = "10.1111/ijfs.12555"
}

Stajić, S., Živković, D., Tomović, V., Nedović, V., Perunović, M., Kovjanić, N., Lević, S.,& Stanišić, N.. (2014). The utilisation of grapeseed oil in improving the quality of dry fermented sausages. in International Journal of Food Science and Technology
Wiley-Blackwell, Hoboken., 49(11), 2356-2363.
https://doi.org/10.1111/ijfs.12555

Stajić S, Živković D, Tomović V, Nedović V, Perunović M, Kovjanić N, Lević S, Stanišić N. The utilisation of grapeseed oil in improving the quality of dry fermented sausages. in International Journal of Food Science and Technology. 2014;49(11):2356-2363.
doi:10.1111/ijfs.12555 .

Stajić, Slaviša, Živković, Dušan, Tomović, Vladimir, Nedović, Viktor, Perunović, Marija, Kovjanić, Nataša, Lević, Steva, Stanišić, Nikola, "The utilisation of grapeseed oil in improving the quality of dry fermented sausages" in International Journal of Food Science and Technology, 49, no. 11 (2014):2356-2363,
https://doi.org/10.1111/ijfs.12555 . .

TY  - JOUR
AU  - Petrović, Tanja S.
AU  - Nedic, Nebojša M.
AU  - Paunović, D.
AU  - Rajić, J.
AU  - Matović, K.
AU  - Radulović, Z.
AU  - Krnjaja, Vesna
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/418
AB  - Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health.
AB  - Ukupan broj gljiva, učestalost (incidenca) gljiva i koncentracija aflatoksina B1 (AFB1) ispitivani su u 33 uzoraka polena sakupljenih od pčelara iz različitih regiona u Srbiji. Ukupan broj gljiva određen je primenom metode razređenja a AFB1 je određen primenom imunoadsorpcione enzimske metode (ELISA). Mikološkim ispitivanjima identifikovano je devet rodova gljiva: Acremonium, Alternaria, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Penicillium i Rhizopus, sa ukupnim brojem od 1 x 103 to 1 x 105 CFU g-1. Najučestalije vrste gljiva su u rodovima Aspergillus i Alternaria. Među Aspergillus vrstama najučestalija je vrsta A. flavus sa incidencom od 27,27%. AFB1 je detektovan u svim uzorcima sa prosečnom koncentracijom od 8,61 μg kg-1. Dobijeni rezultati ukazuju da pčelarski polen mora biti strogo kontrolisan tokom prikupljanja i njegove dalje prerade. Zbog toga, sprovođenje dobre proizvođačke (pčelarske) prakse podrazumeva definisanje procedura za pčelarske proizvode što bi moglo biti presudno za smanjenje rizika od moguće kontaminacije i dobijanje prirodnih i bezbednih proizvoda bez rizika po zdravlje ljudi.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia
T1  - Prirodna mikobiota i prisustvo aflatoksina B1 u polenu prikupljenom u Srbiji
VL  - 30
IS  - 4
SP  - 731
EP  - 741
DO  - 10.2298/BAH1404731P
ER  - 

@article{
author = "Petrović, Tanja S. and Nedic, Nebojša M. and Paunović, D. and Rajić, J. and Matović, K. and Radulović, Z. and Krnjaja, Vesna",
year = "2014",
abstract = "Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health., Ukupan broj gljiva, učestalost (incidenca) gljiva i koncentracija aflatoksina B1 (AFB1) ispitivani su u 33 uzoraka polena sakupljenih od pčelara iz različitih regiona u Srbiji. Ukupan broj gljiva određen je primenom metode razređenja a AFB1 je određen primenom imunoadsorpcione enzimske metode (ELISA). Mikološkim ispitivanjima identifikovano je devet rodova gljiva: Acremonium, Alternaria, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Penicillium i Rhizopus, sa ukupnim brojem od 1 x 103 to 1 x 105 CFU g-1. Najučestalije vrste gljiva su u rodovima Aspergillus i Alternaria. Među Aspergillus vrstama najučestalija je vrsta A. flavus sa incidencom od 27,27%. AFB1 je detektovan u svim uzorcima sa prosečnom koncentracijom od 8,61 μg kg-1. Dobijeni rezultati ukazuju da pčelarski polen mora biti strogo kontrolisan tokom prikupljanja i njegove dalje prerade. Zbog toga, sprovođenje dobre proizvođačke (pčelarske) prakse podrazumeva definisanje procedura za pčelarske proizvode što bi moglo biti presudno za smanjenje rizika od moguće kontaminacije i dobijanje prirodnih i bezbednih proizvoda bez rizika po zdravlje ljudi.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia, Prirodna mikobiota i prisustvo aflatoksina B1 u polenu prikupljenom u Srbiji",
volume = "30",
number = "4",
pages = "731-741",
doi = "10.2298/BAH1404731P"
}

Petrović, T. S., Nedic, N. M., Paunović, D., Rajić, J., Matović, K., Radulović, Z.,& Krnjaja, V.. (2014). Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 30(4), 731-741.
https://doi.org/10.2298/BAH1404731P

Petrović TS, Nedic NM, Paunović D, Rajić J, Matović K, Radulović Z, Krnjaja V. Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia. in Biotechnology in Animal Husbandry. 2014;30(4):731-741.
doi:10.2298/BAH1404731P .

Petrović, Tanja S., Nedic, Nebojša M., Paunović, D., Rajić, J., Matović, K., Radulović, Z., Krnjaja, Vesna, "Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia" in Biotechnology in Animal Husbandry, 30, no. 4 (2014):731-741,
https://doi.org/10.2298/BAH1404731P . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Tomić, Zorica
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://r.istocar.bg.ac.rs/handle/123456789/399
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Tomić, Zorica and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Tomić, Z., Mandić, V.,& Bijelić, Z.. (2013). Moulds and mycotoxins in stored maize grains. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K

Krnjaja V, Lević J, Stanković SŽ, Petrović TS, Tomić Z, Mandić V, Bijelić Z. Moulds and mycotoxins in stored maize grains. in Biotechnology in Animal Husbandry. 2013;29(3):527-536.
doi:10.2298/BAH1303527K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Tomić, Zorica, Mandić, Violeta, Bijelić, Zorica, "Moulds and mycotoxins in stored maize grains" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Lukić, Miloš
PY  - 2013
UR  - http://r.istocar.bg.ac.rs/handle/123456789/372
AB  - Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).
AB  - Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Molds and mycotoxins in freshly harvested maize
T1  - Plesni i mikotoksini u zrnu kukuruza posle berbe
IS  - 124
SP  - 111
EP  - 119
DO  - 10.2298/ZMSPN1324111K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Lukić, Miloš",
year = "2013",
abstract = "Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02)., Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Molds and mycotoxins in freshly harvested maize, Plesni i mikotoksini u zrnu kukuruza posle berbe",
number = "124",
pages = "111-119",
doi = "10.2298/ZMSPN1324111K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž., Petrović, T. S.,& Lukić, M.. (2013). Molds and mycotoxins in freshly harvested maize. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 111-119.
https://doi.org/10.2298/ZMSPN1324111K

Krnjaja V, Lević J, Stanković SŽ, Petrović TS, Lukić M. Molds and mycotoxins in freshly harvested maize. in Zbornik Matice srpske za prirodne nauke. 2013;(124):111-119.
doi:10.2298/ZMSPN1324111K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Lukić, Miloš, "Molds and mycotoxins in freshly harvested maize" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):111-119,
https://doi.org/10.2298/ZMSPN1324111K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Petrović, Tanja S.
AU  - Stojanović, Ljiljana
AU  - Radović, Čedomir
AU  - Gogić, Marija
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/366
AB  - In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54).
AB  - U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Distribution of moulds and mycotoxins in maize grain silage in the trench silo
T1  - Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču
VL  - 28
IS  - 4
SP  - 845
EP  - 854
DO  - 10.2298/BAH1204845K
ER  - 

@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Petrović, Tanja S. and Stojanović, Ljiljana and Radović, Čedomir and Gogić, Marija",
year = "2012",
abstract = "In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54)., U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Distribution of moulds and mycotoxins in maize grain silage in the trench silo, Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču",
volume = "28",
number = "4",
pages = "845-854",
doi = "10.2298/BAH1204845K"
}

Krnjaja, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Stojanović, L., Radović, Č.,& Gogić, M.. (2012). Distribution of moulds and mycotoxins in maize grain silage in the trench silo. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 28(4), 845-854.
https://doi.org/10.2298/BAH1204845K

Krnjaja V, Lević J, Stanković SŽ, Petrović TS, Stojanović L, Radović Č, Gogić M. Distribution of moulds and mycotoxins in maize grain silage in the trench silo. in Biotechnology in Animal Husbandry. 2012;28(4):845-854.
doi:10.2298/BAH1204845K .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Petrović, Tanja S., Stojanović, Ljiljana, Radović, Čedomir, Gogić, Marija, "Distribution of moulds and mycotoxins in maize grain silage in the trench silo" in Biotechnology in Animal Husbandry, 28, no. 4 (2012):845-854,
https://doi.org/10.2298/BAH1204845K . .

TY  - JOUR
AU  - Živković, Dušan
AU  - Tomović, Vladimir
AU  - Perunović, Marija
AU  - Stajić, Slaviša
AU  - Stanišić, Nikola
AU  - Bogićević, Nataša
PY  - 2011
UR  - http://r.istocar.bg.ac.rs/handle/123456789/293
AB  - Dry fermented sausages are one of the most valued groups of meat products. Specific environmental conditions determined the production technology, which has remained mostly unchanged until the present day. These products possess specific, sometimes unique sensory characteristics. One of the most famous product in our country, belonging to this group, is Sremska sausage. Traditionally, Sremska sausage was made from meat of late maturing pig breeds that were slaughtered at the age of over 12 months and which, during the last decades, for well-known reasons, were suppressed by modern breeds and their crossbreeds. Today, meat of pigs of about six months of age, as well as pigs older than 12 months, is used for sausage production. Taste and aroma of dry and fermented products are formed, among other things, by the activity of endogenic proteinases, peptidases and lipase and it is well-known that their levels and activity are conditioned by the age of pigs. Colour intensity and stability, another important sensory parameter indicating the quality of fermented sausages, is closely associated with the colour of meat used in production, while the texture is a result of complex influences, including composition and quality of fatty tissue, dynamics and intensity of pH changes and presence of connective tissue in fresh meat. This paper examines the impact of pig age on biochemical and sensory parameters in Sremska sausage during production and three-month storage period. Meat and fatty tissue used in the experiments were from Swedish Landrace, more specifically six-month old fatteners (variant A) and 12-month old cull sows (variant B). Biochemical changes in sausages made from meat of pigs between six and 12 months of age basically differed only slightly. Dynamics of pH value changes and the content of non-protein nitrogen showed similar tendencies, as well as the electrophoretic profiles of sarcoplasmic and myofibrillar proteins. The impact of pig age on sensory characteristics of Sremska sausage was pronounced. Variant A sausages had the best appearance and texture when compared to variant B on days 14, 60 and 105 of the experiment. The colour of product A was also more appealing during the experiment. As for taste and aroma, variant A was inferior on day 14, but during storage, the taste and aroma of this variant improved, unlike those of variant B, in which these parameters deteriorated. Variant A sausages had much lighter cross-section (P  (lt)  0,05). On the surface and the cross-section of variant A, statistically significantly higher participation of red colour (a*) was registered, than in variant B.
AB  - Suve fermentisane kobasice poseduju specifične, nekada jedinstvene senzorne karakteristike. Sremska kobasica je jedan od najpoznatijih proizvoda iz ove grupe proizvoda u našoj zemlji. Tradicionalno se sremska kobasica proizvodila od mesa kasnostasnih rasa svinja, starijih od 12 meseci, koje su tokom prošlih decenija potisnutle plemenite rase svinja, i njihovi melezi. Danas se, najčešće, koristi meso tovljenika starih oko 6 meseci i meso svinja starijih od 12 meseci. Ukus i miris sušenih i fermentisanih proizvoda formiraju se, između ostalog, aktivnošću endogenih proteinaza, peptidaza i lipaza, a njihov nivo i aktivnost uslovljeni su starošću svinja. Intenzitet i postojanost boje u tesnoj je vezi sa bojom mesa koje se koristi, a tekstura je rezultat složenih uticaja, od kojih se ističu: sastav i kvalitet masnog tkiva, dinamika i intenzitet promena pH vrednosti, kao i razvijenost vezivnotkivnih komponenti sirovine. Ispitivan je uticaj starosti svinja na biohemijske i senzorne parametre sremske kobasice tokom proizvodnje i tromesečnog skladištenja. Korišćeni su meso i masno tkivo svinja rase švedski landras, i to: šestomesečnih tovljenika (varijanta A) i krmača starih 12 meseci izlučenih iz priploda (varijanta B). Biohemijske promene, u osnovi, se malo razlikuju. Dinamika promene pH vrednosti, sadržaj neproteinskog azota i elektroforetski profili sarkoplazmatskih i miofibrilarnih proteina, u obe varijante, pokazuju veoma slične tendencije. Uticaj starosti svinja na senzorne karakteristike ispitivanih varijanti sremske kobasice je izražen. Kobasice varijante A imale su bolji spoljašnji izgled i boju. U pogledu mirisa, varijanta A, je na početku ogleda, bila inferiorna, ali se tokom skladištenja miris ove varijante poboljšavao, za razliku od varijante B, kod koje se miris pogoršavao. U toku čuvanja, ukus varijante B ocenjen je sličnim ocenama, dok se ukus varijante A poboljšao. Na površini i na preseku kobasica varijante A utvrđeno je statistički značajno veće učešće crvene boje, a, takođe, su i značajno veće svetloće na preseku.
PB  - Institute of Meat Hygiene and Technology, Belgrade
T2  - Tehnologija mesa
T1  - Sensory acceptability of 'Sremska' sausage made from meat of pigs of different ages
T1  - Senzorna prihvatljivost sremske kobasice izrađene od mesa svinja različite starosti
VL  - 52
IS  - 2
SP  - 252
EP  - 261
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_293
ER  - 

@article{
author = "Živković, Dušan and Tomović, Vladimir and Perunović, Marija and Stajić, Slaviša and Stanišić, Nikola and Bogićević, Nataša",
year = "2011",
abstract = "Dry fermented sausages are one of the most valued groups of meat products. Specific environmental conditions determined the production technology, which has remained mostly unchanged until the present day. These products possess specific, sometimes unique sensory characteristics. One of the most famous product in our country, belonging to this group, is Sremska sausage. Traditionally, Sremska sausage was made from meat of late maturing pig breeds that were slaughtered at the age of over 12 months and which, during the last decades, for well-known reasons, were suppressed by modern breeds and their crossbreeds. Today, meat of pigs of about six months of age, as well as pigs older than 12 months, is used for sausage production. Taste and aroma of dry and fermented products are formed, among other things, by the activity of endogenic proteinases, peptidases and lipase and it is well-known that their levels and activity are conditioned by the age of pigs. Colour intensity and stability, another important sensory parameter indicating the quality of fermented sausages, is closely associated with the colour of meat used in production, while the texture is a result of complex influences, including composition and quality of fatty tissue, dynamics and intensity of pH changes and presence of connective tissue in fresh meat. This paper examines the impact of pig age on biochemical and sensory parameters in Sremska sausage during production and three-month storage period. Meat and fatty tissue used in the experiments were from Swedish Landrace, more specifically six-month old fatteners (variant A) and 12-month old cull sows (variant B). Biochemical changes in sausages made from meat of pigs between six and 12 months of age basically differed only slightly. Dynamics of pH value changes and the content of non-protein nitrogen showed similar tendencies, as well as the electrophoretic profiles of sarcoplasmic and myofibrillar proteins. The impact of pig age on sensory characteristics of Sremska sausage was pronounced. Variant A sausages had the best appearance and texture when compared to variant B on days 14, 60 and 105 of the experiment. The colour of product A was also more appealing during the experiment. As for taste and aroma, variant A was inferior on day 14, but during storage, the taste and aroma of this variant improved, unlike those of variant B, in which these parameters deteriorated. Variant A sausages had much lighter cross-section (P  (lt)  0,05). On the surface and the cross-section of variant A, statistically significantly higher participation of red colour (a*) was registered, than in variant B., Suve fermentisane kobasice poseduju specifične, nekada jedinstvene senzorne karakteristike. Sremska kobasica je jedan od najpoznatijih proizvoda iz ove grupe proizvoda u našoj zemlji. Tradicionalno se sremska kobasica proizvodila od mesa kasnostasnih rasa svinja, starijih od 12 meseci, koje su tokom prošlih decenija potisnutle plemenite rase svinja, i njihovi melezi. Danas se, najčešće, koristi meso tovljenika starih oko 6 meseci i meso svinja starijih od 12 meseci. Ukus i miris sušenih i fermentisanih proizvoda formiraju se, između ostalog, aktivnošću endogenih proteinaza, peptidaza i lipaza, a njihov nivo i aktivnost uslovljeni su starošću svinja. Intenzitet i postojanost boje u tesnoj je vezi sa bojom mesa koje se koristi, a tekstura je rezultat složenih uticaja, od kojih se ističu: sastav i kvalitet masnog tkiva, dinamika i intenzitet promena pH vrednosti, kao i razvijenost vezivnotkivnih komponenti sirovine. Ispitivan je uticaj starosti svinja na biohemijske i senzorne parametre sremske kobasice tokom proizvodnje i tromesečnog skladištenja. Korišćeni su meso i masno tkivo svinja rase švedski landras, i to: šestomesečnih tovljenika (varijanta A) i krmača starih 12 meseci izlučenih iz priploda (varijanta B). Biohemijske promene, u osnovi, se malo razlikuju. Dinamika promene pH vrednosti, sadržaj neproteinskog azota i elektroforetski profili sarkoplazmatskih i miofibrilarnih proteina, u obe varijante, pokazuju veoma slične tendencije. Uticaj starosti svinja na senzorne karakteristike ispitivanih varijanti sremske kobasice je izražen. Kobasice varijante A imale su bolji spoljašnji izgled i boju. U pogledu mirisa, varijanta A, je na početku ogleda, bila inferiorna, ali se tokom skladištenja miris ove varijante poboljšavao, za razliku od varijante B, kod koje se miris pogoršavao. U toku čuvanja, ukus varijante B ocenjen je sličnim ocenama, dok se ukus varijante A poboljšao. Na površini i na preseku kobasica varijante A utvrđeno je statistički značajno veće učešće crvene boje, a, takođe, su i značajno veće svetloće na preseku.",
publisher = "Institute of Meat Hygiene and Technology, Belgrade",
journal = "Tehnologija mesa",
title = "Sensory acceptability of 'Sremska' sausage made from meat of pigs of different ages, Senzorna prihvatljivost sremske kobasice izrađene od mesa svinja različite starosti",
volume = "52",
number = "2",
pages = "252-261",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_293"
}

Živković, D., Tomović, V., Perunović, M., Stajić, S., Stanišić, N.,& Bogićević, N.. (2011). Sensory acceptability of 'Sremska' sausage made from meat of pigs of different ages. in Tehnologija mesa
Institute of Meat Hygiene and Technology, Belgrade., 52(2), 252-261.
https://hdl.handle.net/21.15107/rcub_ristocar_293

Živković D, Tomović V, Perunović M, Stajić S, Stanišić N, Bogićević N. Sensory acceptability of 'Sremska' sausage made from meat of pigs of different ages. in Tehnologija mesa. 2011;52(2):252-261.
https://hdl.handle.net/21.15107/rcub_ristocar_293 .

Živković, Dušan, Tomović, Vladimir, Perunović, Marija, Stajić, Slaviša, Stanišić, Nikola, Bogićević, Nataša, "Sensory acceptability of 'Sremska' sausage made from meat of pigs of different ages" in Tehnologija mesa, 52, no. 2 (2011):252-261,
https://hdl.handle.net/21.15107/rcub_ristocar_293 .