TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lučev, Milica
AU  - Obradović, Ana
AU  - Mićić, Nenad
PY  - 2024
UR  - https://www.chileanjar.cl/journal.php?V=84&I=5&P=September%20-%20October%202024
UR  - http://r.istocar.bg.ac.rs/handle/123456789/1011
AB  - Fusarium head blight (FHB), caused by different Fusarium species, is the most devastating disease of small cereal grains, including barley (Hordeum vulgare L.). This study aimed to investigate the influence of N fertilization rates (0 kg N ha-1 - N0, 50 kg N ha-1 - N50, and 100 kg N ha-1 - N100) on Fusarium and mycotoxin (deoxynivalenol - DON and zearalenone - ZEA) contamination and some agronomic traits (plant height - PH, spike length - SL and thousand kernel weight - TKW) in two barley cultivars, NS 565 and Etincel, harvested in 2019 and 2020. Climatic conditions during two successive seasons were favourable for Fusarium infection, providing a high incidence of FHB-causing species, of which F. graminearum species complex (FGSC) strains were the most frequent (on average > 34% per treatment). The N rates and barley cultivars had nonsignificant effects on the incidence of FGSC strains. However, N rates significantly influenced mycotoxin levels in 2019, with the highest DON at N100 (5209.67 μg kg-1) and ZEA levels at N50 (47.11 μg kg-1). In 2020, there were nonsignificant differences between N rates for DON levels, while ZEA was not detected. In both years, the six-row barley ‘Etincel’ had significantly higher DON and ZEA levels than the two-row barley ‘NS 565’. Agronomic traits, PH and SL, were affected by barley cultivars, and TKW was affected by N rates and barley cultivars. The highest TKW was at N100, followed by N50 and N0.
PB  - INIA, Agricultural Research Institute, Chile
T2  - Chilean Journal of Agricultural Research
T1  - Fusarium spp. infection, mycotoxin contamination, and some agronomic traits in winter barley as affected by N fertilization under Serbia conditions
VL  - 84
IS  - 5
SP  - 632
EP  - 643
DO  - 10.4067/S0718-58392024000500632
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Petrović, Tanja and Stanković, Slavica and Lučev, Milica and Obradović, Ana and Mićić, Nenad",
year = "2024",
abstract = "Fusarium head blight (FHB), caused by different Fusarium species, is the most devastating disease of small cereal grains, including barley (Hordeum vulgare L.). This study aimed to investigate the influence of N fertilization rates (0 kg N ha-1 - N0, 50 kg N ha-1 - N50, and 100 kg N ha-1 - N100) on Fusarium and mycotoxin (deoxynivalenol - DON and zearalenone - ZEA) contamination and some agronomic traits (plant height - PH, spike length - SL and thousand kernel weight - TKW) in two barley cultivars, NS 565 and Etincel, harvested in 2019 and 2020. Climatic conditions during two successive seasons were favourable for Fusarium infection, providing a high incidence of FHB-causing species, of which F. graminearum species complex (FGSC) strains were the most frequent (on average > 34% per treatment). The N rates and barley cultivars had nonsignificant effects on the incidence of FGSC strains. However, N rates significantly influenced mycotoxin levels in 2019, with the highest DON at N100 (5209.67 μg kg-1) and ZEA levels at N50 (47.11 μg kg-1). In 2020, there were nonsignificant differences between N rates for DON levels, while ZEA was not detected. In both years, the six-row barley ‘Etincel’ had significantly higher DON and ZEA levels than the two-row barley ‘NS 565’. Agronomic traits, PH and SL, were affected by barley cultivars, and TKW was affected by N rates and barley cultivars. The highest TKW was at N100, followed by N50 and N0.",
publisher = "INIA, Agricultural Research Institute, Chile",
journal = "Chilean Journal of Agricultural Research",
title = "Fusarium spp. infection, mycotoxin contamination, and some agronomic traits in winter barley as affected by N fertilization under Serbia conditions",
volume = "84",
number = "5",
pages = "632-643",
doi = "10.4067/S0718-58392024000500632"
}

Krnjaja, V., Mandić, V., Petrović, T., Stanković, S., Lučev, M., Obradović, A.,& Mićić, N.. (2024). Fusarium spp. infection, mycotoxin contamination, and some agronomic traits in winter barley as affected by N fertilization under Serbia conditions. in Chilean Journal of Agricultural Research
INIA, Agricultural Research Institute, Chile., 84(5), 632-643.
https://doi.org/10.4067/S0718-58392024000500632

Krnjaja V, Mandić V, Petrović T, Stanković S, Lučev M, Obradović A, Mićić N. Fusarium spp. infection, mycotoxin contamination, and some agronomic traits in winter barley as affected by N fertilization under Serbia conditions. in Chilean Journal of Agricultural Research. 2024;84(5):632-643.
doi:10.4067/S0718-58392024000500632 .

Krnjaja, Vesna, Mandić, Violeta, Petrović, Tanja, Stanković, Slavica, Lučev, Milica, Obradović, Ana, Mićić, Nenad, "Fusarium spp. infection, mycotoxin contamination, and some agronomic traits in winter barley as affected by N fertilization under Serbia conditions" in Chilean Journal of Agricultural Research, 84, no. 5 (2024):632-643,
https://doi.org/10.4067/S0718-58392024000500632 . .

TY  - CONF
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Lučev, Milica
AU  - Stanković, Slavica
PY  - 2024
UR  - http://r.istocar.bg.ac.rs/handle/123456789/986
AB  - The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.
PB  - University of Zagreb Faculty of Agriculture, Zagreb, Croatia
C3  - Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
T1  - Trichothecene chemotypes of Fusarium graminearum species complex in Serbia
SP  - 225
EP  - 225
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_986
ER  - 

@conference{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Lučev, Milica and Stanković, Slavica",
year = "2024",
abstract = "The species Fusarium graminearum represents one of the most economically important factor that threaten agricultural production in the world. In addition to affecting yield reduction and grain quality, this species is of great importance due to its ability to synthesize mycotoxins. Knowing the toxicological profile of F. graminearum species is essential for agriculture and food industry, because trichothecene chemotypes (NIV, 3ADON, 15ADON) have different toxicological properties that directly affect human and animal health. In this study, 20 isolates of F. graminearum originating from maize and wheat were examined. DNA isolation was performed from the mycelium of the fungus using the commercial kit Dneasy Plant Mini Kit according to the manufacturer’s instructions. Chemotype detection was performed using two sets of specific primers (TRI3 and TRI12) designed for trichothecenes by Ward et al. in 2002. After the polymerase chain reaction was completed, the products were electrophoretically separated on a 1% agarose gel, and visualization was performed by staining with ethidium bromide and observing under UV light. Amplified fragments were read visually, and a positive reaction indicated the presence of DNA fragment of the expected size. All isolates of F. graminearum examined in this study had products of size 610bp at TRI3 and 670bp at TRI12, which indicates that isolates originating from Serbia belonged to 15ADON chemotype.",
publisher = "University of Zagreb Faculty of Agriculture, Zagreb, Croatia",
journal = "Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia",
title = "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia",
pages = "225-225",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_986"
}

Obradović, A., Stepanović, J., Krnjaja, V., Lučev, M.,& Stanković, S.. (2024). Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia
University of Zagreb Faculty of Agriculture, Zagreb, Croatia., 225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986

Obradović A, Stepanović J, Krnjaja V, Lučev M, Stanković S. Trichothecene chemotypes of Fusarium graminearum species complex in Serbia. in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia. 2024;:225-225.
https://hdl.handle.net/21.15107/rcub_ristocar_986 .

Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Lučev, Milica, Stanković, Slavica, "Trichothecene chemotypes of Fusarium graminearum species complex in Serbia" in Book of Abstracts of 59th Croatian & 19th International Symposium on Agriculture, 11 – 16 February 2024, Dubrovnik, Croatia (2024):225-225,
https://hdl.handle.net/21.15107/rcub_ristocar_986 .