TY  - RPRT
AU  - Dadousis, Christos
AU  - Munoz, Maria
AU  - Ovilo, Cristina
AU  - Fabbri, Maria Chiara
AU  - Araujo, Jose Pedro
AU  - Bovo, Samuele
AU  - Čandek Potokar, Marjeta
AU  - Charneca, Rui
AU  - Crovetti, Alessandro
AU  - Gallo, Maurizio
AU  - Garcia-Casco, Juan Maria
AU  - Karolyi, Danijel
AU  - Kušec, Goran
AU  - Martins, Jose Manuel
AU  - Mercat, Marie-Jose
AU  - Pugliese, Carolina
AU  - Quintanilla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Ribani, Anisa
AU  - Raquet, Juliet
AU  - Savić, Radomir
AU  - Schiavo, Giuseppina
AU  - Škrlep, Martin
AU  - Tinarelli, Silvia
AU  - Usai, Graziano
AU  - Zimmer, Christoph
AU  - Fontanesi, Luca
AU  - Bozzi, Riccardo
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/819
AB  - Preserving diversity of indigenous pig (Sus scrofa) breeds is a key factor to (i) sustain the pork chain
(both at local and global scales) including the production of high-quality branded products, (ii) enrich
the animal biobanking and (iii) progress conservation policies. Single nucleotide polymorphism (SNP)
chips ofer the opportunity for whole-genome comparisons among individuals and breeds. Animals
from twenty European local pigs breeds, reared in nine countries (Croatia: Black Slavonian, Turopolje;
France: Basque, Gascon; Germany: Schwabisch-Hällisches Schwein; Italy: Apulo Calabrese, Casertana,
Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda; Lithuania: Indigenous Wattle, White Old Type;
Portugal: Alentejana, Bísara; Serbia: Moravka, Swallow-Bellied Mangalitsa; Slovenia: Krškopolje pig;
Spain: Iberian, Majorcan Black), and three commercial breeds (Duroc, Landrace and Large White) were
sampled and genotyped with the GeneSeek Genomic Profler (GGP) 70 K HD porcine genotyping chip.
A dataset of 51 Wild Boars from nine countries was also added, summing up to 1186 pigs (~49 pigs/
breed). The aim was to: (i) investigate individual admixture ancestries and (ii) assess breed traceability
via discriminant analysis on principal components (DAPC). Albeit the mosaic of shared ancestries
found for Nero Siciliano, Sarda and Moravka, admixture analysis indicated independent evolvement
PB  - Nature Portfolio
T2  - Scientifc Reports
T1  - Admixture and breed traceability in European indigenous pig breeds and wild boar using genome‑wide SNP data
VL  - 12
IS  - 7146
DO  - https://doi.org/10.1038/s41598-022-10698-8
ER  - 

@techreport{
author = "Dadousis, Christos and Munoz, Maria and Ovilo, Cristina and Fabbri, Maria Chiara and Araujo, Jose Pedro and Bovo, Samuele and Čandek Potokar, Marjeta and Charneca, Rui and Crovetti, Alessandro and Gallo, Maurizio and Garcia-Casco, Juan Maria and Karolyi, Danijel and Kušec, Goran and Martins, Jose Manuel and Mercat, Marie-Jose and Pugliese, Carolina and Quintanilla, Raquel and Radović, Čedomir and Razmaite, Violeta and Ribani, Anisa and Raquet, Juliet and Savić, Radomir and Schiavo, Giuseppina and Škrlep, Martin and Tinarelli, Silvia and Usai, Graziano and Zimmer, Christoph and Fontanesi, Luca and Bozzi, Riccardo",
year = "2022",
abstract = "Preserving diversity of indigenous pig (Sus scrofa) breeds is a key factor to (i) sustain the pork chain
(both at local and global scales) including the production of high-quality branded products, (ii) enrich
the animal biobanking and (iii) progress conservation policies. Single nucleotide polymorphism (SNP)
chips ofer the opportunity for whole-genome comparisons among individuals and breeds. Animals
from twenty European local pigs breeds, reared in nine countries (Croatia: Black Slavonian, Turopolje;
France: Basque, Gascon; Germany: Schwabisch-Hällisches Schwein; Italy: Apulo Calabrese, Casertana,
Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda; Lithuania: Indigenous Wattle, White Old Type;
Portugal: Alentejana, Bísara; Serbia: Moravka, Swallow-Bellied Mangalitsa; Slovenia: Krškopolje pig;
Spain: Iberian, Majorcan Black), and three commercial breeds (Duroc, Landrace and Large White) were
sampled and genotyped with the GeneSeek Genomic Profler (GGP) 70 K HD porcine genotyping chip.
A dataset of 51 Wild Boars from nine countries was also added, summing up to 1186 pigs (~49 pigs/
breed). The aim was to: (i) investigate individual admixture ancestries and (ii) assess breed traceability
via discriminant analysis on principal components (DAPC). Albeit the mosaic of shared ancestries
found for Nero Siciliano, Sarda and Moravka, admixture analysis indicated independent evolvement",
publisher = "Nature Portfolio",
journal = "Scientifc Reports",
title = "Admixture and breed traceability in European indigenous pig breeds and wild boar using genome‑wide SNP data",
volume = "12",
number = "7146",
doi = "https://doi.org/10.1038/s41598-022-10698-8"
}

Dadousis, C., Munoz, M., Ovilo, C., Fabbri, M. C., Araujo, J. P., Bovo, S., Čandek Potokar, M., Charneca, R., Crovetti, A., Gallo, M., Garcia-Casco, J. M., Karolyi, D., Kušec, G., Martins, J. M., Mercat, M., Pugliese, C., Quintanilla, R., Radović, Č., Razmaite, V., Ribani, A., Raquet, J., Savić, R., Schiavo, G., Škrlep, M., Tinarelli, S., Usai, G., Zimmer, C., Fontanesi, L.,& Bozzi, R.. (2022). Admixture and breed traceability in European indigenous pig breeds and wild boar using genome‑wide SNP data. in Scientifc Reports
Nature Portfolio., 12(7146).
https://doi.org/https://doi.org/10.1038/s41598-022-10698-8

Dadousis C, Munoz M, Ovilo C, Fabbri MC, Araujo JP, Bovo S, Čandek Potokar M, Charneca R, Crovetti A, Gallo M, Garcia-Casco JM, Karolyi D, Kušec G, Martins JM, Mercat M, Pugliese C, Quintanilla R, Radović Č, Razmaite V, Ribani A, Raquet J, Savić R, Schiavo G, Škrlep M, Tinarelli S, Usai G, Zimmer C, Fontanesi L, Bozzi R. Admixture and breed traceability in European indigenous pig breeds and wild boar using genome‑wide SNP data. in Scientifc Reports. 2022;12(7146).
doi:https://doi.org/10.1038/s41598-022-10698-8 .

Dadousis, Christos, Munoz, Maria, Ovilo, Cristina, Fabbri, Maria Chiara, Araujo, Jose Pedro, Bovo, Samuele, Čandek Potokar, Marjeta, Charneca, Rui, Crovetti, Alessandro, Gallo, Maurizio, Garcia-Casco, Juan Maria, Karolyi, Danijel, Kušec, Goran, Martins, Jose Manuel, Mercat, Marie-Jose, Pugliese, Carolina, Quintanilla, Raquel, Radović, Čedomir, Razmaite, Violeta, Ribani, Anisa, Raquet, Juliet, Savić, Radomir, Schiavo, Giuseppina, Škrlep, Martin, Tinarelli, Silvia, Usai, Graziano, Zimmer, Christoph, Fontanesi, Luca, Bozzi, Riccardo, "Admixture and breed traceability in European indigenous pig breeds and wild boar using genome‑wide SNP data" in Scientifc Reports, 12, no. 7146 (2022),
https://doi.org/https://doi.org/10.1038/s41598-022-10698-8 . .

TY  - JOUR
AU  - Zorc, Minja
AU  - Škorput, Dubravko
AU  - Gvozdanović, Kristina
AU  - Margeta, Polona
AU  - Karolyi, Danijel
AU  - Luković, Zoran
AU  - Salajpal, Krešimir
AU  - Savić, Radomir
AU  - Munoz, Maria
AU  - Bovo, Samuele
AU  - Đurkin Kušec, Ivona
AU  - Radović, Čedomir
AU  - Kušec, Goran
AU  - Čandek Potokar, Marjeta
AU  - Dovč, Peter
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/818
AB  - Background: The importance of local breeds as genetic reservoirs of valuable genetic variation is well established.
Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local
pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from
Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka
and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide
polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were
compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and
identifed genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS).
Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka
and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed.
The analysis of admixture between breeds revealed potential gene fow between the Mangalitsa and Moravka, and
between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and
Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identifed
genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which
harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS
approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance.
Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the
existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to
estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed
genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under
selection pressure.
PB  - BMC
T2  - Genetic Selection Evolution
T1  - Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia
DO  - https://doi.org/10.1186/s12711-022-00718-6
ER  - 

@article{
author = "Zorc, Minja and Škorput, Dubravko and Gvozdanović, Kristina and Margeta, Polona and Karolyi, Danijel and Luković, Zoran and Salajpal, Krešimir and Savić, Radomir and Munoz, Maria and Bovo, Samuele and Đurkin Kušec, Ivona and Radović, Čedomir and Kušec, Goran and Čandek Potokar, Marjeta and Dovč, Peter",
year = "2022",
abstract = "Background: The importance of local breeds as genetic reservoirs of valuable genetic variation is well established.
Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local
pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from
Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka
and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide
polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were
compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and
identifed genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS).
Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka
and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed.
The analysis of admixture between breeds revealed potential gene fow between the Mangalitsa and Moravka, and
between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and
Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identifed
genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which
harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS
approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance.
Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the
existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to
estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed
genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under
selection pressure.",
publisher = "BMC",
journal = "Genetic Selection Evolution",
title = "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia",
doi = "https://doi.org/10.1186/s12711-022-00718-6"
}

Zorc, M., Škorput, D., Gvozdanović, K., Margeta, P., Karolyi, D., Luković, Z., Salajpal, K., Savić, R., Munoz, M., Bovo, S., Đurkin Kušec, I., Radović, Č., Kušec, G., Čandek Potokar, M.,& Dovč, P.. (2022). Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetic Selection Evolution
BMC..
https://doi.org/https://doi.org/10.1186/s12711-022-00718-6

Zorc M, Škorput D, Gvozdanović K, Margeta P, Karolyi D, Luković Z, Salajpal K, Savić R, Munoz M, Bovo S, Đurkin Kušec I, Radović Č, Kušec G, Čandek Potokar M, Dovč P. Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetic Selection Evolution. 2022;.
doi:https://doi.org/10.1186/s12711-022-00718-6 .

Zorc, Minja, Škorput, Dubravko, Gvozdanović, Kristina, Margeta, Polona, Karolyi, Danijel, Luković, Zoran, Salajpal, Krešimir, Savić, Radomir, Munoz, Maria, Bovo, Samuele, Đurkin Kušec, Ivona, Radović, Čedomir, Kušec, Goran, Čandek Potokar, Marjeta, Dovč, Peter, "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia" in Genetic Selection Evolution (2022),
https://doi.org/https://doi.org/10.1186/s12711-022-00718-6 . .

TY  - JOUR
AU  - Schiavo, Giussepina
AU  - Bovo, Samuele
AU  - Munoz, Maria
AU  - Ribani, Anisa
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Čandek-Potokar, Marjeta
AU  - Charneca, Rui
AU  - Fernandez, Ana I.
AU  - Gallo, Maurizio
AU  - Garcia, Fabian
AU  - Karolyi, Danijel
AU  - Kušec, Goran
AU  - Martins, Jose Manuel
AU  - Mercat, Marie-Jose
AU  - Nunez, Yolanda
AU  - Quintanilla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Usai, Graziano
AU  - Utzeri, Valerio J.
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2021-04
UR  - http://r.istocar.bg.ac.rs/handle/123456789/925
AB  - ROHs are long stretches of DNA homozygous at each polymorphic position. The proportion of
genome covered by ROHs and their length are indicators of the level and origin of inbreeding.
Frequent common ROHs within the same population define ROH islands and indicate hotspots of
selection. In this work, we investigated ROHs in a total of 1131 pigs from 20 European local pig
breeds and in three cosmopolitan breeds, genotyped with the GGP Porcine HD Genomic Profiler.
PLINK software was used to identify ROHs. Size classes and genomic inbreeding parameters were evaluated. ROH islands were defined by evaluating different thresholds of homozygous SNP
frequency. A functional overview of breed-specific ROH islands was obtained via overrepresentation analyses of GO biological processes. Mora Romagnola and Turopolje breeds had the largest proportions of genome covered with ROH (~1003 and ~955 Mb respectively),
whereas Nero Siciliano and Sarda breeds had the lowest proportions (~207 and 247 Mb
respectively). The highest proportion of long ROH (>16 Mb) was in Apulo-Calabrese, Mora
Romagnola and Casertana. The largest number of ROH islands was identified in the Italian
Landrace (n=32), Cinta Senese (n=26) and LithuanianWhite Old Type (n=22) breeds. Several
ROH islands were in regions encompassing genes known to affect morphological traits.
Comparative ROH structure analysis among breeds indicated the similar genetic structure of
local breeds across Europe. This study contributed to understanding of the genetic history of the
investigated pig breeds and provided information to manage these pig genetic resources.
PB  - Blackwell Publishing Ltd
T2  - Animal Genetics
T1  - Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds
VL  - 52
SP  - 155
EP  - 170
DO  - 10.1111/age.13045
ER  - 

@article{
author = "Schiavo, Giussepina and Bovo, Samuele and Munoz, Maria and Ribani, Anisa and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Čandek-Potokar, Marjeta and Charneca, Rui and Fernandez, Ana I. and Gallo, Maurizio and Garcia, Fabian and Karolyi, Danijel and Kušec, Goran and Martins, Jose Manuel and Mercat, Marie-Jose and Nunez, Yolanda and Quintanilla, Raquel and Radović, Čedomir and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Usai, Graziano and Utzeri, Valerio J. and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca",
year = "2021-04",
abstract = "ROHs are long stretches of DNA homozygous at each polymorphic position. The proportion of
genome covered by ROHs and their length are indicators of the level and origin of inbreeding.
Frequent common ROHs within the same population define ROH islands and indicate hotspots of
selection. In this work, we investigated ROHs in a total of 1131 pigs from 20 European local pig
breeds and in three cosmopolitan breeds, genotyped with the GGP Porcine HD Genomic Profiler.
PLINK software was used to identify ROHs. Size classes and genomic inbreeding parameters were evaluated. ROH islands were defined by evaluating different thresholds of homozygous SNP
frequency. A functional overview of breed-specific ROH islands was obtained via overrepresentation analyses of GO biological processes. Mora Romagnola and Turopolje breeds had the largest proportions of genome covered with ROH (~1003 and ~955 Mb respectively),
whereas Nero Siciliano and Sarda breeds had the lowest proportions (~207 and 247 Mb
respectively). The highest proportion of long ROH (>16 Mb) was in Apulo-Calabrese, Mora
Romagnola and Casertana. The largest number of ROH islands was identified in the Italian
Landrace (n=32), Cinta Senese (n=26) and LithuanianWhite Old Type (n=22) breeds. Several
ROH islands were in regions encompassing genes known to affect morphological traits.
Comparative ROH structure analysis among breeds indicated the similar genetic structure of
local breeds across Europe. This study contributed to understanding of the genetic history of the
investigated pig breeds and provided information to manage these pig genetic resources.",
publisher = "Blackwell Publishing Ltd",
journal = "Animal Genetics",
title = "Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds",
volume = "52",
pages = "155-170",
doi = "10.1111/age.13045"
}

Schiavo, G., Bovo, S., Munoz, M., Ribani, A., Alves, E., Araujo, J. P., Bozzi, R., Čandek-Potokar, M., Charneca, R., Fernandez, A. I., Gallo, M., Garcia, F., Karolyi, D., Kušec, G., Martins, J. M., Mercat, M., Nunez, Y., Quintanilla, R., Radović, Č., Razmaite, V., Riquet, J., Savić, R., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2021-04). Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds. in Animal Genetics
Blackwell Publishing Ltd., 52, 155-170.
https://doi.org/10.1111/age.13045

Schiavo G, Bovo S, Munoz M, Ribani A, Alves E, Araujo JP, Bozzi R, Čandek-Potokar M, Charneca R, Fernandez AI, Gallo M, Garcia F, Karolyi D, Kušec G, Martins JM, Mercat M, Nunez Y, Quintanilla R, Radović Č, Razmaite V, Riquet J, Savić R, Usai G, Utzeri VJ, Zimmer C, Ovilo C, Fontanesi L. Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds. in Animal Genetics. 2021;52:155-170.
doi:10.1111/age.13045 .

Schiavo, Giussepina, Bovo, Samuele, Munoz, Maria, Ribani, Anisa, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Čandek-Potokar, Marjeta, Charneca, Rui, Fernandez, Ana I., Gallo, Maurizio, Garcia, Fabian, Karolyi, Danijel, Kušec, Goran, Martins, Jose Manuel, Mercat, Marie-Jose, Nunez, Yolanda, Quintanilla, Raquel, Radović, Čedomir, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Usai, Graziano, Utzeri, Valerio J., Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, "Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds" in Animal Genetics, 52 (2021-04):155-170,
https://doi.org/10.1111/age.13045 . .

TY  - JOUR
AU  - Nunez, Yolanda
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Garcia-Casco, M. Juan
AU  - Čandek-Potokar, Marjeta
AU  - Benitez, Rita
AU  - Radojković, Dragan
AU  - Lukić, Miloš
AU  - Gogić, Marija
AU  - Munoz, Maria
AU  - Fontanesi, Luca
AU  - Ovilo, Cristina
PY  - 2021-03-16
UR  - http://r.istocar.bg.ac.rs/handle/123456789/772
AB  - This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under
identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA-seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT- Ds, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
PB  - MDPI
T2  - Animals
T1  - Muscle transcriptome analysis reveals molecular pathways related to oxidative phosphorylation, antioxidant defense, fatness and growth in mangalitsa and moravka pigs
VL  - 11
IS  - 844
DO  - 10.3390/ani11030844
ER  - 

@article{
author = "Nunez, Yolanda and Radović, Čedomir and Savić, Radomir and Garcia-Casco, M. Juan and Čandek-Potokar, Marjeta and Benitez, Rita and Radojković, Dragan and Lukić, Miloš and Gogić, Marija and Munoz, Maria and Fontanesi, Luca and Ovilo, Cristina",
year = "2021-03-16",
abstract = "This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under
identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA-seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT- Ds, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.",
publisher = "MDPI",
journal = "Animals",
title = "Muscle transcriptome analysis reveals molecular pathways related to oxidative phosphorylation, antioxidant defense, fatness and growth in mangalitsa and moravka pigs",
volume = "11",
number = "844",
doi = "10.3390/ani11030844"
}

Nunez, Y., Radović, Č., Savić, R., Garcia-Casco, M. J., Čandek-Potokar, M., Benitez, R., Radojković, D., Lukić, M., Gogić, M., Munoz, M., Fontanesi, L.,& Ovilo, C.. (2021-03-16). Muscle transcriptome analysis reveals molecular pathways related to oxidative phosphorylation, antioxidant defense, fatness and growth in mangalitsa and moravka pigs. in Animals
MDPI., 11(844).
https://doi.org/10.3390/ani11030844

Nunez Y, Radović Č, Savić R, Garcia-Casco MJ, Čandek-Potokar M, Benitez R, Radojković D, Lukić M, Gogić M, Munoz M, Fontanesi L, Ovilo C. Muscle transcriptome analysis reveals molecular pathways related to oxidative phosphorylation, antioxidant defense, fatness and growth in mangalitsa and moravka pigs. in Animals. 2021;11(844).
doi:10.3390/ani11030844 .

Nunez, Yolanda, Radović, Čedomir, Savić, Radomir, Garcia-Casco, M. Juan, Čandek-Potokar, Marjeta, Benitez, Rita, Radojković, Dragan, Lukić, Miloš, Gogić, Marija, Munoz, Maria, Fontanesi, Luca, Ovilo, Cristina, "Muscle transcriptome analysis reveals molecular pathways related to oxidative phosphorylation, antioxidant defense, fatness and growth in mangalitsa and moravka pigs" in Animals, 11, no. 844 (2021-03-16),
https://doi.org/10.3390/ani11030844 . .

TY  - JOUR
AU  - Bovo, Samuele
AU  - Ribani, Anisa
AU  - Munoz, Maria
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Čandek-Potokar, Marjeta
AU  - Charneca, Rui
AU  - Di Palma, Federica
AU  - Etherington, Graham
AU  - Fernandez, Ana I.
AU  - Garcia, Fabian
AU  - Garcia-Casco, Juan
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Margeta, Vladimir
AU  - Martins, Jose Manuel
AU  - Mercat, Marie J.
AU  - Moscatelli, Giulia
AU  - Nunaz, Yolanda
AU  - Quintailla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Schiavo, Giuseppina
AU  - Usai, Graziano
AU  - Utzeri, Valerio J.
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/718
AB  - Background: Natural and artificial directional selection in cosmopolitan and autochthonous pig breeds and wild boars have shaped their genomes and resulted in a reservoir of animal genetic diversity. Signatures of selection are the result of these selection events that have contributed to the adaptation of breeds to different environments and production systems. In this study, we analysed the genome variability of 19 European autochthonous pig breeds (Alentejana, Bísara, Majorcan Black, Basque, Gascon, Apulo‑Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda, Krškopolje pig, Black Slavonian, Turopolje, Moravka, Swallow‑Bellied Mangalitsa, Schwäbisch‑Hällisches Schwein, Lithuanian indigenous wattle and Lithuanian White old type) from nine countries, three European commercial breeds (Italian Large White, Italian Landrace and Italian Duroc), and European wild boars, by mining whole‑genome sequencing data obtained by using a DNA‑pool sequencing approach. Signatures of selection were identified by using a single‑breed approach with two statistics [within‑breed pooled heterozygosity (HP) and fixation index (FST)] and group‑based FST approaches, which compare groups of breeds defined according to external traits and use/specialization/type. Results: We detected more than 22 million single nucleotide polymorphisms (SNPs) across the 23 compared populations and identified 359 chromosome regions showing signatures of selection. These regions harbour genes that are already known or new genes that are under selection and relevant for the domestication process in this species, and that affect several morphological and physiological traits (e.g. coat colours and patterns, body size, number of vertebrae and teats, ear size and conformation, reproductive traits, growth and fat deposition traits). Wild boar related signatures of selection were detected across all the genome of several autochthonous breeds, which suggests that crossbreeding (accidental or deliberate) occurred with wild boars.
Conclusions: Our findings provide a catalogue of genetic variants of many European pig populations and identify genome regions that can explain, at least in part, the phenotypic diversity of these genetic resources.
PB  - BMC Publishing
T2  - Genetics Selection Evolution
T1  - Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems
VL  - 52
IS  - 33
DO  - doi.org/10.1186/s12711-020-00553-7
ER  - 

@article{
author = "Bovo, Samuele and Ribani, Anisa and Munoz, Maria and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Čandek-Potokar, Marjeta and Charneca, Rui and Di Palma, Federica and Etherington, Graham and Fernandez, Ana I. and Garcia, Fabian and Garcia-Casco, Juan and Karolyi, Danijel and Gallo, Maurizio and Margeta, Vladimir and Martins, Jose Manuel and Mercat, Marie J. and Moscatelli, Giulia and Nunaz, Yolanda and Quintailla, Raquel and Radović, Čedomir and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Schiavo, Giuseppina and Usai, Graziano and Utzeri, Valerio J. and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca",
year = "2020",
abstract = "Background: Natural and artificial directional selection in cosmopolitan and autochthonous pig breeds and wild boars have shaped their genomes and resulted in a reservoir of animal genetic diversity. Signatures of selection are the result of these selection events that have contributed to the adaptation of breeds to different environments and production systems. In this study, we analysed the genome variability of 19 European autochthonous pig breeds (Alentejana, Bísara, Majorcan Black, Basque, Gascon, Apulo‑Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda, Krškopolje pig, Black Slavonian, Turopolje, Moravka, Swallow‑Bellied Mangalitsa, Schwäbisch‑Hällisches Schwein, Lithuanian indigenous wattle and Lithuanian White old type) from nine countries, three European commercial breeds (Italian Large White, Italian Landrace and Italian Duroc), and European wild boars, by mining whole‑genome sequencing data obtained by using a DNA‑pool sequencing approach. Signatures of selection were identified by using a single‑breed approach with two statistics [within‑breed pooled heterozygosity (HP) and fixation index (FST)] and group‑based FST approaches, which compare groups of breeds defined according to external traits and use/specialization/type. Results: We detected more than 22 million single nucleotide polymorphisms (SNPs) across the 23 compared populations and identified 359 chromosome regions showing signatures of selection. These regions harbour genes that are already known or new genes that are under selection and relevant for the domestication process in this species, and that affect several morphological and physiological traits (e.g. coat colours and patterns, body size, number of vertebrae and teats, ear size and conformation, reproductive traits, growth and fat deposition traits). Wild boar related signatures of selection were detected across all the genome of several autochthonous breeds, which suggests that crossbreeding (accidental or deliberate) occurred with wild boars.
Conclusions: Our findings provide a catalogue of genetic variants of many European pig populations and identify genome regions that can explain, at least in part, the phenotypic diversity of these genetic resources.",
publisher = "BMC Publishing",
journal = "Genetics Selection Evolution",
title = "Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems",
volume = "52",
number = "33",
doi = "doi.org/10.1186/s12711-020-00553-7"
}

Bovo, S., Ribani, A., Munoz, M., Alves, E., Araujo, J. P., Bozzi, R., Čandek-Potokar, M., Charneca, R., Di Palma, F., Etherington, G., Fernandez, A. I., Garcia, F., Garcia-Casco, J., Karolyi, D., Gallo, M., Margeta, V., Martins, J. M., Mercat, M. J., Moscatelli, G., Nunaz, Y., Quintailla, R., Radović, Č., Razmaite, V., Riquet, J., Savić, R., Schiavo, G., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2020). Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems. in Genetics Selection Evolution
BMC Publishing., 52(33).
https://doi.org/doi.org/10.1186/s12711-020-00553-7

Bovo S, Ribani A, Munoz M, Alves E, Araujo JP, Bozzi R, Čandek-Potokar M, Charneca R, Di Palma F, Etherington G, Fernandez AI, Garcia F, Garcia-Casco J, Karolyi D, Gallo M, Margeta V, Martins JM, Mercat MJ, Moscatelli G, Nunaz Y, Quintailla R, Radović Č, Razmaite V, Riquet J, Savić R, Schiavo G, Usai G, Utzeri VJ, Zimmer C, Ovilo C, Fontanesi L. Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems. in Genetics Selection Evolution. 2020;52(33).
doi:doi.org/10.1186/s12711-020-00553-7 .

Bovo, Samuele, Ribani, Anisa, Munoz, Maria, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Čandek-Potokar, Marjeta, Charneca, Rui, Di Palma, Federica, Etherington, Graham, Fernandez, Ana I., Garcia, Fabian, Garcia-Casco, Juan, Karolyi, Danijel, Gallo, Maurizio, Margeta, Vladimir, Martins, Jose Manuel, Mercat, Marie J., Moscatelli, Giulia, Nunaz, Yolanda, Quintailla, Raquel, Radović, Čedomir, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Schiavo, Giuseppina, Usai, Graziano, Utzeri, Valerio J., Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, "Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems" in Genetics Selection Evolution, 52, no. 33 (2020),
https://doi.org/doi.org/10.1186/s12711-020-00553-7 . .

TY  - JOUR
AU  - Gogić, Marija
AU  - Radović, Čedomir
AU  - Čandek-Potokar, Marjeta
AU  - Petrović, Milica
AU  - Radojković, Dragan
AU  - Parunović, Nenad
AU  - Savić, Radomir
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/667
AB  - The objective of this research was to study the effect of immunocastration
on the morphometric characteristics of sex glands in male Swallow-bellied Mangalitsa
pigs. The study included 24 animals (13 entire and 11 immunocastrated males). The
research focused on the following traits: age at slaughter (days), live weight at slaughter
(kg), growth rate (kg day−1), and morphometric testicle traits (volume, cm3; weight, g;
length, width, thickness, and circumference, mm). The efficacy of immunocastration
was determined based on androstenone and skatole levels in adipose tissue. The
group of immunocastrated animals had higher body weight at slaughter (+18.8 kg)
due to higher average daily live gain (+87 g). Immunocastration resulted in reduced
morphometric measures of testes. The volumes and weight of testes/epididymes were
reduced by 28.9-54.4 and 32.1-53.3%, respectively. Testis length and other size-related
traits were significantly reduced by 17.4-27.6%. The linear regression effect of live body
weight at slaughter within the immunocastrated group on testis measurements was not
significant. Within the entire group of males, the increase of live body weight at slaughter
by 1 kg had an effect on the increase of volume and weight of testes/epididymes by
0.55-2.24 cm3 and 0.52-2.28 g, respectively. Other size-related testis traits increased
by 0.04-0.55 mm for each kg of body weight. Immunocastration also resulted in
79.6% decrease in the weight of accessory sex glands. The present study is the first to
demonstrate that immunocastration could be a method of choice for castration of the
local fatty pig breed, Mangulica (Swallow-bellied Mangalitsa).
PB  - Sociedade Brasileira de Zootecnia, Brazil
T2  - Revista brasileira de zootecnia-Brazilian journal of animal science
T1  - Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs
VL  - 48
IS  - 48:e20180286
DO  - //dx.doi.org/10.1590/rbz4820180286
ER  - 

@article{
author = "Gogić, Marija and Radović, Čedomir and Čandek-Potokar, Marjeta and Petrović, Milica and Radojković, Dragan and Parunović, Nenad and Savić, Radomir",
year = "2019",
abstract = "The objective of this research was to study the effect of immunocastration
on the morphometric characteristics of sex glands in male Swallow-bellied Mangalitsa
pigs. The study included 24 animals (13 entire and 11 immunocastrated males). The
research focused on the following traits: age at slaughter (days), live weight at slaughter
(kg), growth rate (kg day−1), and morphometric testicle traits (volume, cm3; weight, g;
length, width, thickness, and circumference, mm). The efficacy of immunocastration
was determined based on androstenone and skatole levels in adipose tissue. The
group of immunocastrated animals had higher body weight at slaughter (+18.8 kg)
due to higher average daily live gain (+87 g). Immunocastration resulted in reduced
morphometric measures of testes. The volumes and weight of testes/epididymes were
reduced by 28.9-54.4 and 32.1-53.3%, respectively. Testis length and other size-related
traits were significantly reduced by 17.4-27.6%. The linear regression effect of live body
weight at slaughter within the immunocastrated group on testis measurements was not
significant. Within the entire group of males, the increase of live body weight at slaughter
by 1 kg had an effect on the increase of volume and weight of testes/epididymes by
0.55-2.24 cm3 and 0.52-2.28 g, respectively. Other size-related testis traits increased
by 0.04-0.55 mm for each kg of body weight. Immunocastration also resulted in
79.6% decrease in the weight of accessory sex glands. The present study is the first to
demonstrate that immunocastration could be a method of choice for castration of the
local fatty pig breed, Mangulica (Swallow-bellied Mangalitsa).",
publisher = "Sociedade Brasileira de Zootecnia, Brazil",
journal = "Revista brasileira de zootecnia-Brazilian journal of animal science",
title = "Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs",
volume = "48",
number = "48:e20180286",
doi = "//dx.doi.org/10.1590/rbz4820180286"
}

Gogić, M., Radović, Č., Čandek-Potokar, M., Petrović, M., Radojković, D., Parunović, N.,& Savić, R.. (2019). Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs. in Revista brasileira de zootecnia-Brazilian journal of animal science
Sociedade Brasileira de Zootecnia, Brazil., 48(48:e20180286).
https://doi.org///dx.doi.org/10.1590/rbz4820180286

Gogić M, Radović Č, Čandek-Potokar M, Petrović M, Radojković D, Parunović N, Savić R. Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs. in Revista brasileira de zootecnia-Brazilian journal of animal science. 2019;48(48:e20180286).
doi://dx.doi.org/10.1590/rbz4820180286 .

Gogić, Marija, Radović, Čedomir, Čandek-Potokar, Marjeta, Petrović, Milica, Radojković, Dragan, Parunović, Nenad, Savić, Radomir, "Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs" in Revista brasileira de zootecnia-Brazilian journal of animal science, 48, no. 48:e20180286 (2019),
https://doi.org///dx.doi.org/10.1590/rbz4820180286 . .

TY  - JOUR
AU  - Brossard, Ludovic
AU  - Nieto, Rosa
AU  - Charneca, Rui
AU  - Araujo, José Pedro
AU  - Pugliese, Carolina
AU  - Radović, Čedomir
AU  - Čandek-Potokar, Marjeta
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/644
AB  - Models such as InraPorc enable the growth of pigs to be simulated and their nutrient
requirements to be determined. However, so far, these models have not been applied to local breeds.
We used InraPorc to determine the nutrient requirements of growing pigs from local breeds (H2020
European Union project TREASURE). Data on feed composition, allowance and intake, and body
weight (BW) were obtained from literature reports or experiments conducted within the project. Data
were used in InraPorc to calibrate 16 growth and intake profiles from nine breeds (Alentejana, Basque,
Bísara, Apulo Calabrese, Cinta Senese, Iberian, Krškopolje pig, Mangalitsa, and Moravka), with one
to three profiles per breed depending on the experimental conditions or data source. On the 40–100 kg
BW range, mean protein deposition (PDm) was low for all breeds (below 116 g/d vs. over 130 g/d
in conventional breeds). The age of pigs at 40 kg BW (110 to 206 days) denoted different types of
feeding management in addition to genetic differences. The PDm and the lysine requirements were
the highest in breeds with the highest average daily gain. In all breeds, a small proportion of total
body energy retention was dedicated to protein, with the greatest proportion of energy retention in
the form of lipids. Despite some methodological limitations, this study provides initial insights into
the nutrient requirements of some local breeds.
PB  - MDPI Open Access Journals
T2  - Animals
T1  - Modelling Nutritional Requirements of Growing Pigs from Local Breeds Using InraPorc
IS  - 169
SP  - 169
DO  - doi.org/10.3390/ani9040169
ER  - 

@article{
author = "Brossard, Ludovic and Nieto, Rosa and Charneca, Rui and Araujo, José Pedro and Pugliese, Carolina and Radović, Čedomir and Čandek-Potokar, Marjeta",
year = "2019",
abstract = "Models such as InraPorc enable the growth of pigs to be simulated and their nutrient
requirements to be determined. However, so far, these models have not been applied to local breeds.
We used InraPorc to determine the nutrient requirements of growing pigs from local breeds (H2020
European Union project TREASURE). Data on feed composition, allowance and intake, and body
weight (BW) were obtained from literature reports or experiments conducted within the project. Data
were used in InraPorc to calibrate 16 growth and intake profiles from nine breeds (Alentejana, Basque,
Bísara, Apulo Calabrese, Cinta Senese, Iberian, Krškopolje pig, Mangalitsa, and Moravka), with one
to three profiles per breed depending on the experimental conditions or data source. On the 40–100 kg
BW range, mean protein deposition (PDm) was low for all breeds (below 116 g/d vs. over 130 g/d
in conventional breeds). The age of pigs at 40 kg BW (110 to 206 days) denoted different types of
feeding management in addition to genetic differences. The PDm and the lysine requirements were
the highest in breeds with the highest average daily gain. In all breeds, a small proportion of total
body energy retention was dedicated to protein, with the greatest proportion of energy retention in
the form of lipids. Despite some methodological limitations, this study provides initial insights into
the nutrient requirements of some local breeds.",
publisher = "MDPI Open Access Journals",
journal = "Animals",
title = "Modelling Nutritional Requirements of Growing Pigs from Local Breeds Using InraPorc",
number = "169",
pages = "169",
doi = "doi.org/10.3390/ani9040169"
}

Brossard, L., Nieto, R., Charneca, R., Araujo, J. P., Pugliese, C., Radović, Č.,& Čandek-Potokar, M.. (2019). Modelling Nutritional Requirements of Growing Pigs from Local Breeds Using InraPorc. in Animals
MDPI Open Access Journals.(169), 169.
https://doi.org/doi.org/10.3390/ani9040169

Brossard L, Nieto R, Charneca R, Araujo JP, Pugliese C, Radović Č, Čandek-Potokar M. Modelling Nutritional Requirements of Growing Pigs from Local Breeds Using InraPorc. in Animals. 2019;(169):169.
doi:doi.org/10.3390/ani9040169 .

Brossard, Ludovic, Nieto, Rosa, Charneca, Rui, Araujo, José Pedro, Pugliese, Carolina, Radović, Čedomir, Čandek-Potokar, Marjeta, "Modelling Nutritional Requirements of Growing Pigs from Local Breeds Using InraPorc" in Animals, no. 169 (2019):169,
https://doi.org/doi.org/10.3390/ani9040169 . .

TY  - JOUR
AU  - Muñoz, M.
AU  - Bozzi, Riccardo
AU  - García, F.
AU  - Nuñez, Yolanda
AU  - Geraci, Claudia
AU  - Crovetti, Alessandro
AU  - Garcia Casco, Juan Maria
AU  - Alves, Estefânia
AU  - Škrlep, M.
AU  - Charneca, Rui
AU  - Martins, J.M.
AU  - Quintanilla, R.
AU  - Tibau, J.
AU  - Kušec, Goran
AU  - Djurkin-Kušec, I.
AU  - Mercat, Marie-José
AU  - Riquet, J.
AU  - Estellé, J.
AU  - Zimmer, C.
AU  - Razmaite, V.
AU  - Araujo, José Pedro
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Čandek-Potokar, Marjeta
AU  - Fontanesi, Luca
AU  - Fernández, A.I.
AU  - Óvilo, C.
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/551
UR  - http://hdl.handle.net/20.500.12327/251
AB  - The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population's structure and relationships.
PB  - Public Library of Science (PLoS)
T2  - PloS one
T1  - Diversity across major and candidate genes in European local pig breeds
VL  - 13
IS  - 11
DO  - 10.1371/journal.pone.0207475
ER  - 

@article{
author = "Muñoz, M. and Bozzi, Riccardo and García, F. and Nuñez, Yolanda and Geraci, Claudia and Crovetti, Alessandro and Garcia Casco, Juan Maria and Alves, Estefânia and Škrlep, M. and Charneca, Rui and Martins, J.M. and Quintanilla, R. and Tibau, J. and Kušec, Goran and Djurkin-Kušec, I. and Mercat, Marie-José and Riquet, J. and Estellé, J. and Zimmer, C. and Razmaite, V. and Araujo, José Pedro and Radović, Čedomir and Savić, Radomir and Karolyi, Danijel and Gallo, Maurizio and Čandek-Potokar, Marjeta and Fontanesi, Luca and Fernández, A.I. and Óvilo, C.",
year = "2018",
abstract = "The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population's structure and relationships.",
publisher = "Public Library of Science (PLoS)",
journal = "PloS one",
title = "Diversity across major and candidate genes in European local pig breeds",
volume = "13",
number = "11",
doi = "10.1371/journal.pone.0207475"
}

Muñoz, M., Bozzi, R., García, F., Nuñez, Y., Geraci, C., Crovetti, A., Garcia Casco, J. M., Alves, E., Škrlep, M., Charneca, R., Martins, J.M., Quintanilla, R., Tibau, J., Kušec, G., Djurkin-Kušec, I., Mercat, M., Riquet, J., Estellé, J., Zimmer, C., Razmaite, V., Araujo, J. P., Radović, Č., Savić, R., Karolyi, D., Gallo, M., Čandek-Potokar, M., Fontanesi, L., Fernández, A.I.,& Óvilo, C.. (2018). Diversity across major and candidate genes in European local pig breeds. in PloS one
Public Library of Science (PLoS)., 13(11).
https://doi.org/10.1371/journal.pone.0207475

Muñoz M, Bozzi R, García F, Nuñez Y, Geraci C, Crovetti A, Garcia Casco JM, Alves E, Škrlep M, Charneca R, Martins J, Quintanilla R, Tibau J, Kušec G, Djurkin-Kušec I, Mercat M, Riquet J, Estellé J, Zimmer C, Razmaite V, Araujo JP, Radović Č, Savić R, Karolyi D, Gallo M, Čandek-Potokar M, Fontanesi L, Fernández A, Óvilo C. Diversity across major and candidate genes in European local pig breeds. in PloS one. 2018;13(11).
doi:10.1371/journal.pone.0207475 .

Muñoz, M., Bozzi, Riccardo, García, F., Nuñez, Yolanda, Geraci, Claudia, Crovetti, Alessandro, Garcia Casco, Juan Maria, Alves, Estefânia, Škrlep, M., Charneca, Rui, Martins, J.M., Quintanilla, R., Tibau, J., Kušec, Goran, Djurkin-Kušec, I., Mercat, Marie-José, Riquet, J., Estellé, J., Zimmer, C., Razmaite, V., Araujo, José Pedro, Radović, Čedomir, Savić, Radomir, Karolyi, Danijel, Gallo, Maurizio, Čandek-Potokar, Marjeta, Fontanesi, Luca, Fernández, A.I., Óvilo, C., "Diversity across major and candidate genes in European local pig breeds" in PloS one, 13, no. 11 (2018),
https://doi.org/10.1371/journal.pone.0207475 . .

TY  - JOUR
AU  - Čandek-Potokar, Marjeta
AU  - Nieto, R.
AU  - Pugliese, C.
AU  - Araujo, José Pedro
AU  - Charneca, Rui
AU  - Garcia Casco, Juan Maria
AU  - González Sánchez, Elena
AU  - Hernandez-Garcia, Francisco I.
AU  - Izquierdo, Mercedes
AU  - Karolyi, Danijel
AU  - Kušec, Goran
AU  - Lebret, Benedicte
AU  - Mercat, Marie-José
AU  - Petig, M.
AU  - Radović, Čedomir
AU  - Savić, Radomir
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/520
UR  - https://hrcak.srce.hr/191807
AB  - TREASURE is a research and innovation project of European Union’s Horizon 2020 programme which is devoted to traditional genetic resources in pig production with aim to improve their potentials for enhanced use. Studying and improving management of local pig breeds in their production systems is one of the challenges in which we address their performances and nutrition with special attention on locally available feeding resources and innovative practices aiming to improved welfare. For that purpose 15 experiments on 12 breeds were designed in the project, which are hereafter shortly presented. Their concepts and main objectives with some highlights on already available results are described. © 2017, University of Zagreb. All rights reserved.
PB  - Zagreb : Faculty of Agriculture, University of Zagreb
T2  - Agriculturae Conspectus Scientificus
T1  - Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE
VL  - 82
IS  - 2 Special Issue 1
SP  - 127
EP  - 131
DO  - 10.5281/zenodo.1136173
ER  - 

@article{
author = "Čandek-Potokar, Marjeta and Nieto, R. and Pugliese, C. and Araujo, José Pedro and Charneca, Rui and Garcia Casco, Juan Maria and González Sánchez, Elena and Hernandez-Garcia, Francisco I. and Izquierdo, Mercedes and Karolyi, Danijel and Kušec, Goran and Lebret, Benedicte and Mercat, Marie-José and Petig, M. and Radović, Čedomir and Savić, Radomir",
year = "2017",
abstract = "TREASURE is a research and innovation project of European Union’s Horizon 2020 programme which is devoted to traditional genetic resources in pig production with aim to improve their potentials for enhanced use. Studying and improving management of local pig breeds in their production systems is one of the challenges in which we address their performances and nutrition with special attention on locally available feeding resources and innovative practices aiming to improved welfare. For that purpose 15 experiments on 12 breeds were designed in the project, which are hereafter shortly presented. Their concepts and main objectives with some highlights on already available results are described. © 2017, University of Zagreb. All rights reserved.",
publisher = "Zagreb : Faculty of Agriculture, University of Zagreb",
journal = "Agriculturae Conspectus Scientificus",
title = "Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE",
volume = "82",
number = "2 Special Issue 1",
pages = "127-131",
doi = "10.5281/zenodo.1136173"
}

Čandek-Potokar, M., Nieto, R., Pugliese, C., Araujo, J. P., Charneca, R., Garcia Casco, J. M., González Sánchez, E., Hernandez-Garcia, F. I., Izquierdo, M., Karolyi, D., Kušec, G., Lebret, B., Mercat, M., Petig, M., Radović, Č.,& Savić, R.. (2017). Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE. in Agriculturae Conspectus Scientificus
Zagreb : Faculty of Agriculture, University of Zagreb., 82(2 Special Issue 1), 127-131.
https://doi.org/10.5281/zenodo.1136173

Čandek-Potokar M, Nieto R, Pugliese C, Araujo JP, Charneca R, Garcia Casco JM, González Sánchez E, Hernandez-Garcia FI, Izquierdo M, Karolyi D, Kušec G, Lebret B, Mercat M, Petig M, Radović Č, Savić R. Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE. in Agriculturae Conspectus Scientificus. 2017;82(2 Special Issue 1):127-131.
doi:10.5281/zenodo.1136173 .

Čandek-Potokar, Marjeta, Nieto, R., Pugliese, C., Araujo, José Pedro, Charneca, Rui, Garcia Casco, Juan Maria, González Sánchez, Elena, Hernandez-Garcia, Francisco I., Izquierdo, Mercedes, Karolyi, Danijel, Kušec, Goran, Lebret, Benedicte, Mercat, Marie-José, Petig, M., Radović, Čedomir, Savić, Radomir, "Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE" in Agriculturae Conspectus Scientificus, 82, no. 2 Special Issue 1 (2017):127-131,
https://doi.org/10.5281/zenodo.1136173 . .

TY  - CONF
AU  - Savić, Radomir
AU  - Petrović, Milica
AU  - Gogić, Marija
AU  - Radović, Čedomir
AU  - Radojković, Dragan
AU  - Stanišić, Nikola
AU  - Čandek-Potokar, Marjeta
PY  - 2017
UR  - https://zenodo.org/record/1029874
UR  - http://epserbia.open.ac.rs/123456789/189
UR  - http://r.istocar.bg.ac.rs/handle/123456789/575
AB  - The main objective of this paper is to present characteristics of autochthonous pig breed Moravka, reared in the previous century, as well as results of investigations of production performance in the last ten years. A collection of available literature data on reproductive, growth, carcass and meat quality traits of Moravka breed was carried out. According to research studies done in the 50-ies of the last century, Moravka females reached sexual maturity at about 5-6 month, whereas breeding maturity was at age of 10-12 months. Average litter size (different parities) was 6.8-8.2 born alive piglets (1.2 kg birth weight). Lactation lasted around 60 days. At weaning piglets weighed 8.7-10.4 kg (i.e. 120-150 g daily gain). Recent studies show that in average the age at first farrowing was 373±10 days, litter size 8.6±1.7 born piglets, and duration of lactation 46.8±3.4 days. Regarding growing/fattening results, only one of old studies reported daily gain in growing phase which was in average 186-197 g (depending of sex), whereas depending on type of feeding and season daily gain for fattening phase was reported between 490 and 660 g (for pigs on feed mixture and intake between 2.5 to 3.2 kg). Reported feed conversion ratio was from 4.4 to 5.1 kg/kg feed mixtures. A more recent research reported piglets to have 31.9 kg at 192 days denoting a growth rate of about 160 g/day in growing phase, whereas pigs fattened from 32 to 94 kg with maize grew 385 g/day (reported feed conversion ratio was 3.74). Backfat thickness (average of measurements along split line) was app. 7 cm (from about 4 to 9 cm) in earlier and app. 4 cm (from about 3 to 6 cm) in recent studies, but it must be noted that slaughter weight was considerably lower in recent studies. The newest research on Longissimus dorsi muscle showed intramuscular fat content of 7.0±0.6%, 21.5±0.2% of proteins with the specific quality traits (pH45=6.53±0.13, pH24=5.65±0.05; CIE L*=48.9±1.4, a*=12.0±1.0, b*=5.9±0.4).
PB  - Belgrade : Institute for Animal Husbandry
C3  - 11th International Symposium "Modern Trends in Livestock Production", Belgrade, Serbia, 11-13 October 2017
T1  - Productive Traits of Moravka Breed - Has Anything Changed in Last Sixty Years?
DO  - 10.5281/zenodo.1029874
ER  - 

@conference{
author = "Savić, Radomir and Petrović, Milica and Gogić, Marija and Radović, Čedomir and Radojković, Dragan and Stanišić, Nikola and Čandek-Potokar, Marjeta",
year = "2017",
abstract = "The main objective of this paper is to present characteristics of autochthonous pig breed Moravka, reared in the previous century, as well as results of investigations of production performance in the last ten years. A collection of available literature data on reproductive, growth, carcass and meat quality traits of Moravka breed was carried out. According to research studies done in the 50-ies of the last century, Moravka females reached sexual maturity at about 5-6 month, whereas breeding maturity was at age of 10-12 months. Average litter size (different parities) was 6.8-8.2 born alive piglets (1.2 kg birth weight). Lactation lasted around 60 days. At weaning piglets weighed 8.7-10.4 kg (i.e. 120-150 g daily gain). Recent studies show that in average the age at first farrowing was 373±10 days, litter size 8.6±1.7 born piglets, and duration of lactation 46.8±3.4 days. Regarding growing/fattening results, only one of old studies reported daily gain in growing phase which was in average 186-197 g (depending of sex), whereas depending on type of feeding and season daily gain for fattening phase was reported between 490 and 660 g (for pigs on feed mixture and intake between 2.5 to 3.2 kg). Reported feed conversion ratio was from 4.4 to 5.1 kg/kg feed mixtures. A more recent research reported piglets to have 31.9 kg at 192 days denoting a growth rate of about 160 g/day in growing phase, whereas pigs fattened from 32 to 94 kg with maize grew 385 g/day (reported feed conversion ratio was 3.74). Backfat thickness (average of measurements along split line) was app. 7 cm (from about 4 to 9 cm) in earlier and app. 4 cm (from about 3 to 6 cm) in recent studies, but it must be noted that slaughter weight was considerably lower in recent studies. The newest research on Longissimus dorsi muscle showed intramuscular fat content of 7.0±0.6%, 21.5±0.2% of proteins with the specific quality traits (pH45=6.53±0.13, pH24=5.65±0.05; CIE L*=48.9±1.4, a*=12.0±1.0, b*=5.9±0.4).",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "11th International Symposium "Modern Trends in Livestock Production", Belgrade, Serbia, 11-13 October 2017",
title = "Productive Traits of Moravka Breed - Has Anything Changed in Last Sixty Years?",
doi = "10.5281/zenodo.1029874"
}

Savić, R., Petrović, M., Gogić, M., Radović, Č., Radojković, D., Stanišić, N.,& Čandek-Potokar, M.. (2017). Productive Traits of Moravka Breed - Has Anything Changed in Last Sixty Years?. in 11th International Symposium "Modern Trends in Livestock Production", Belgrade, Serbia, 11-13 October 2017
Belgrade : Institute for Animal Husbandry..
https://doi.org/10.5281/zenodo.1029874

Savić R, Petrović M, Gogić M, Radović Č, Radojković D, Stanišić N, Čandek-Potokar M. Productive Traits of Moravka Breed - Has Anything Changed in Last Sixty Years?. in 11th International Symposium "Modern Trends in Livestock Production", Belgrade, Serbia, 11-13 October 2017. 2017;.
doi:10.5281/zenodo.1029874 .

Savić, Radomir, Petrović, Milica, Gogić, Marija, Radović, Čedomir, Radojković, Dragan, Stanišić, Nikola, Čandek-Potokar, Marjeta, "Productive Traits of Moravka Breed - Has Anything Changed in Last Sixty Years?" in 11th International Symposium "Modern Trends in Livestock Production", Belgrade, Serbia, 11-13 October 2017 (2017),
https://doi.org/10.5281/zenodo.1029874 . .

TY  - CONF
AU  - Radović, Čedomir
AU  - Petrović, Milica
AU  - Čandek-Potokar, Marjeta
AU  - Savić, Radomir
AU  - Parunović, Nenad
AU  - Radojković, Dragan
AU  - Gogić, Marija
AU  - Izquierdo, Mercedes
PY  - 2017
UR  - https://zenodo.org/record/1135200
UR  - http://epserbia.open.ac.rs/123456789/197
UR  - http://r.istocar.bg.ac.rs/handle/123456789/573
AB  - Extremely rich genetic funds of animals obtained by natural selection through the centuries are very important resources of Serbia. In Serbia, surgical castration of male animals in first week post partum is a usual practice to avoid boar taint. Considering that Mangalitsa sows are good mothers and most aggressively protect the piglets we tested the alternative to physical castration. The aim of this study was to examine the effect of method of castration (physical or immunocastration) on growth potential of indigenous pig breed Mangalitsa Swallow Bellied. Investigation included surgically castrated (SC; n=12) and immunocastrated (IC; n=11) male pigs, fed ad libitum a feed mixture with 12-13 MJ ME/kg; 13-15% crude protein (two-phase feeding). The experiment started at 24.4 weeks of age when average body  weight (ABW) of SC pigs was 24.3 kg and of IC pigs 28.4 kg. Immunocastrates were vaccinated with Improvac® (Zoetis-Pfizer) at 39 (V1) and revacinated at 46 (V2) weeks of age (WA). Pigs were slaughtered 6 weeks after V2. Until V1 when IC are actually entire males, growth rate was slightly lower in IC than SC pigs (446 vs. 495 g/day, respectively).  Established difference was statistically significant (P=0.03). In the period between V1 and V2 growth rate was alike in IC and SC (454 vs. 448 g/day, respectively; P=0.92). Overalluntil V2, there was no difference in ABW between IC and SC pigs (99.2 vs. 100.2, respectively; P=0.89). The maximal growth rate (689 vs. 683 g/d for SC and IC, respectively) was observed between 35 and 39 WA. In the period after V2 until the end of the fattening period growth rate was significantly higher (P=0.002) in IC than SC pigs (548 g vs. 392 g, respectively) in agreement with higher feed intake of IC than SC (3.2 vs. 2.7 kg/pig/day recorded on a pen basis). Present results on the indigenous Mangalitsa breed confirm what has been previously demonstrated in modern breeds, i.e. that after the effective immunisation the pigs increase their feed intake and consequently growth rate.
PB  - Badajoz : CICYTEX
C3  - Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
T1  - Effect of Castration Method on Growth Rate of Indigenous Pig Breed Mangalitsa Swallow Bellie
SP  - 141
EP  - 142
DO  - 10.5281/zenodo.1135200
ER  - 

@conference{
author = "Radović, Čedomir and Petrović, Milica and Čandek-Potokar, Marjeta and Savić, Radomir and Parunović, Nenad and Radojković, Dragan and Gogić, Marija and Izquierdo, Mercedes",
year = "2017",
abstract = "Extremely rich genetic funds of animals obtained by natural selection through the centuries are very important resources of Serbia. In Serbia, surgical castration of male animals in first week post partum is a usual practice to avoid boar taint. Considering that Mangalitsa sows are good mothers and most aggressively protect the piglets we tested the alternative to physical castration. The aim of this study was to examine the effect of method of castration (physical or immunocastration) on growth potential of indigenous pig breed Mangalitsa Swallow Bellied. Investigation included surgically castrated (SC; n=12) and immunocastrated (IC; n=11) male pigs, fed ad libitum a feed mixture with 12-13 MJ ME/kg; 13-15% crude protein (two-phase feeding). The experiment started at 24.4 weeks of age when average body  weight (ABW) of SC pigs was 24.3 kg and of IC pigs 28.4 kg. Immunocastrates were vaccinated with Improvac® (Zoetis-Pfizer) at 39 (V1) and revacinated at 46 (V2) weeks of age (WA). Pigs were slaughtered 6 weeks after V2. Until V1 when IC are actually entire males, growth rate was slightly lower in IC than SC pigs (446 vs. 495 g/day, respectively).  Established difference was statistically significant (P=0.03). In the period between V1 and V2 growth rate was alike in IC and SC (454 vs. 448 g/day, respectively; P=0.92). Overalluntil V2, there was no difference in ABW between IC and SC pigs (99.2 vs. 100.2, respectively; P=0.89). The maximal growth rate (689 vs. 683 g/d for SC and IC, respectively) was observed between 35 and 39 WA. In the period after V2 until the end of the fattening period growth rate was significantly higher (P=0.002) in IC than SC pigs (548 g vs. 392 g, respectively) in agreement with higher feed intake of IC than SC (3.2 vs. 2.7 kg/pig/day recorded on a pen basis). Present results on the indigenous Mangalitsa breed confirm what has been previously demonstrated in modern breeds, i.e. that after the effective immunisation the pigs increase their feed intake and consequently growth rate.",
publisher = "Badajoz : CICYTEX",
journal = "Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference",
title = "Effect of Castration Method on Growth Rate of Indigenous Pig Breed Mangalitsa Swallow Bellie",
pages = "141-142",
doi = "10.5281/zenodo.1135200"
}

Radović, Č., Petrović, M., Čandek-Potokar, M., Savić, R., Parunović, N., Radojković, D., Gogić, M.,& Izquierdo, M.. (2017). Effect of Castration Method on Growth Rate of Indigenous Pig Breed Mangalitsa Swallow Bellie. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
Badajoz : CICYTEX., 141-142.
https://doi.org/10.5281/zenodo.1135200

Radović Č, Petrović M, Čandek-Potokar M, Savić R, Parunović N, Radojković D, Gogić M, Izquierdo M. Effect of Castration Method on Growth Rate of Indigenous Pig Breed Mangalitsa Swallow Bellie. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference. 2017;:141-142.
doi:10.5281/zenodo.1135200 .

Radović, Čedomir, Petrović, Milica, Čandek-Potokar, Marjeta, Savić, Radomir, Parunović, Nenad, Radojković, Dragan, Gogić, Marija, Izquierdo, Mercedes, "Effect of Castration Method on Growth Rate of Indigenous Pig Breed Mangalitsa Swallow Bellie" in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference (2017):141-142,
https://doi.org/10.5281/zenodo.1135200 . .

TY  - CONF
AU  - Savić, Radomir
AU  - Petrović, Milica
AU  - Radović, Čedomir
AU  - Parunović, Nenad
AU  - Radojković, Dragan
AU  - Stanišić, Nikola
AU  - Gogić, Marija
AU  - Čandek-Potokar, Marjeta
PY  - 2017
UR  - https://zenodo.org/record/1135217
UR  - http://epserbia.open.ac.rs/123456789/198
UR  - http://r.istocar.bg.ac.rs/handle/123456789/571
AB  - Fatty acids composition of m. longissimus dorsi (MLD) of Moravka pigs was analyzed considering also the effect of sex and body weight of pigs at slaughter. Pigs were reared in facilities with the openaire section and fed a complete feed mixtures adapted to the stage of growth (from 20-60 kg mixture I: 15.50% of crude protein (CP) and 12.95 MJ metabolisable energy (ME)/kg,; from 60-120 kg mixture II: 13.00% CP and 13.05 MJ ME/kg). The study included 21 pigs (12 castrated males and 9 females). Pigs were slaughtered at 339±30 days of age and 112.8±19.9 kg of live weight. MLD of Moravka pigs had the following fatty acids composition: 41.8 % saturated fatty acids (ΣSFA), 54.0% monounsaturated fatty acids (ΣMUFA) and 4.1% polyunsaturated fatty acids (ΣPUFA) resulting in value 0.10 for the ratio of polyunsaturated to saturated fatty acids (P/S). Fatty acids C16:0 and C18:0 represented the largest share of ΣSFA (61.7 and 34.3 %, respectively), C18:1 and C16:1 the largest share of ΣMUFA (90.1 and 7.1%, respectively), and C18:2 the largest share of ΣPUFA (93.5%). With regard to sex effect, castrated males exhibited higher content of saturated fatty acids C14:0 (1.42 vs. 1.26, P=0.046) and C18:0 (15.07 vs. 13.38, P=0.025) than females. Significant effect (P<0.05) of body weight was observed on some fatty acids; thus 1 kg increase of slaughter weight was accompanied with 0.036% decrease of linoleic acid (C18:2n-6), 0.038% decrease of total content of polyunsaturated acids (ΣPUFA), 0.020% increase of palmitoleic acid (C16:1cis-9), 0.067% increase in total content of monounsaturated fatty acids (ΣMUFA), 0.003% increase of C17:0. Ratio P/S decreased by 0.001 unit per kg increase of slaughter weight. In agreement with their high relative content, a strong correlation (rP=0.79 and rP=0.77) was found between C16:0 and C18:0 and total content of saturated fatty acids (ΣSFA), between C16:1cis-9 and C18:1cis-9 and ΣMUFA (rP=0.80 and rP=0.98, p<0.001), and between linoleic acid (C18:2n-6) and ΣPUFA (rP=0.99, p<0.001).
PB  - Badajoz : CICYTEX
C3  - Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
T1  - Fatty Acids Content of M. Longissimus Dorsi of Moravka Pigs
SP  - 143
EP  - 144
DO  - 10.5281/zenodo.1135217
ER  - 

@conference{
author = "Savić, Radomir and Petrović, Milica and Radović, Čedomir and Parunović, Nenad and Radojković, Dragan and Stanišić, Nikola and Gogić, Marija and Čandek-Potokar, Marjeta",
year = "2017",
abstract = "Fatty acids composition of m. longissimus dorsi (MLD) of Moravka pigs was analyzed considering also the effect of sex and body weight of pigs at slaughter. Pigs were reared in facilities with the openaire section and fed a complete feed mixtures adapted to the stage of growth (from 20-60 kg mixture I: 15.50% of crude protein (CP) and 12.95 MJ metabolisable energy (ME)/kg,; from 60-120 kg mixture II: 13.00% CP and 13.05 MJ ME/kg). The study included 21 pigs (12 castrated males and 9 females). Pigs were slaughtered at 339±30 days of age and 112.8±19.9 kg of live weight. MLD of Moravka pigs had the following fatty acids composition: 41.8 % saturated fatty acids (ΣSFA), 54.0% monounsaturated fatty acids (ΣMUFA) and 4.1% polyunsaturated fatty acids (ΣPUFA) resulting in value 0.10 for the ratio of polyunsaturated to saturated fatty acids (P/S). Fatty acids C16:0 and C18:0 represented the largest share of ΣSFA (61.7 and 34.3 %, respectively), C18:1 and C16:1 the largest share of ΣMUFA (90.1 and 7.1%, respectively), and C18:2 the largest share of ΣPUFA (93.5%). With regard to sex effect, castrated males exhibited higher content of saturated fatty acids C14:0 (1.42 vs. 1.26, P=0.046) and C18:0 (15.07 vs. 13.38, P=0.025) than females. Significant effect (P<0.05) of body weight was observed on some fatty acids; thus 1 kg increase of slaughter weight was accompanied with 0.036% decrease of linoleic acid (C18:2n-6), 0.038% decrease of total content of polyunsaturated acids (ΣPUFA), 0.020% increase of palmitoleic acid (C16:1cis-9), 0.067% increase in total content of monounsaturated fatty acids (ΣMUFA), 0.003% increase of C17:0. Ratio P/S decreased by 0.001 unit per kg increase of slaughter weight. In agreement with their high relative content, a strong correlation (rP=0.79 and rP=0.77) was found between C16:0 and C18:0 and total content of saturated fatty acids (ΣSFA), between C16:1cis-9 and C18:1cis-9 and ΣMUFA (rP=0.80 and rP=0.98, p<0.001), and between linoleic acid (C18:2n-6) and ΣPUFA (rP=0.99, p<0.001).",
publisher = "Badajoz : CICYTEX",
journal = "Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference",
title = "Fatty Acids Content of M. Longissimus Dorsi of Moravka Pigs",
pages = "143-144",
doi = "10.5281/zenodo.1135217"
}

Savić, R., Petrović, M., Radović, Č., Parunović, N., Radojković, D., Stanišić, N., Gogić, M.,& Čandek-Potokar, M.. (2017). Fatty Acids Content of M. Longissimus Dorsi of Moravka Pigs. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
Badajoz : CICYTEX., 143-144.
https://doi.org/10.5281/zenodo.1135217

Savić R, Petrović M, Radović Č, Parunović N, Radojković D, Stanišić N, Gogić M, Čandek-Potokar M. Fatty Acids Content of M. Longissimus Dorsi of Moravka Pigs. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference. 2017;:143-144.
doi:10.5281/zenodo.1135217 .

Savić, Radomir, Petrović, Milica, Radović, Čedomir, Parunović, Nenad, Radojković, Dragan, Stanišić, Nikola, Gogić, Marija, Čandek-Potokar, Marjeta, "Fatty Acids Content of M. Longissimus Dorsi of Moravka Pigs" in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference (2017):143-144,
https://doi.org/10.5281/zenodo.1135217 . .

TY  - JOUR
AU  - Radović, Čedomir
AU  - Petrović, M.
AU  - Savić, Radomir
AU  - Gogić, Marija
AU  - Lukić, Miloš
AU  - Stanišić, Nikola
AU  - Čandek-Potokar, Marjeta
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/517
AB  - The aim of this study was to examine the growth potential of indigenous pig breeds Mangalitsa (swallow bellied; n=12) and Moravka (n=10). The investigation included male castrates (surgical). Pigs were transferred from different farms to the institute’s stable where pigs were housed. The fattening period started at an average weight of 24.3 kg and 173.1 days of age for Mangalitsa pigs and 29.9 and 170.8 days for Moravka pigs, respectively and finished at average weight of 115.5 kg and 357 days of age for Mangalitsa pigs and 131.3 kg and 363 days for Moravka pigs, respectively. During observation study Moravka pigs had 13% higher (but not significantly) growth rate than Mangalitsa breed (545 vs. 480 g/day, P=0.22). The maximal growth rate observed for Mangalitsa was 690 g and for Moravka 607 g in the period corresponding to average body weight (end of period) of 79 and 89 kg, respectively. Initial (at start of the study) difference in growth rate between Mangalitsa and Moravka was propagated along the study duration resulting in significant difference in body weight at slaughter (P (lt) 0.05). © 2017, University of Zagreb. All rights reserved.
T2  - Agriculturae Conspectus Scientificus
T1  - Growth potential of Serbian local pig breeds Mangalitsa and Moravka
VL  - 82
IS  - 3 Special Issue 2
SP  - 217
EP  - 220
DO  - 10.5281/zenodo.1136193
ER  - 

@article{
author = "Radović, Čedomir and Petrović, M. and Savić, Radomir and Gogić, Marija and Lukić, Miloš and Stanišić, Nikola and Čandek-Potokar, Marjeta",
year = "2017",
abstract = "The aim of this study was to examine the growth potential of indigenous pig breeds Mangalitsa (swallow bellied; n=12) and Moravka (n=10). The investigation included male castrates (surgical). Pigs were transferred from different farms to the institute’s stable where pigs were housed. The fattening period started at an average weight of 24.3 kg and 173.1 days of age for Mangalitsa pigs and 29.9 and 170.8 days for Moravka pigs, respectively and finished at average weight of 115.5 kg and 357 days of age for Mangalitsa pigs and 131.3 kg and 363 days for Moravka pigs, respectively. During observation study Moravka pigs had 13% higher (but not significantly) growth rate than Mangalitsa breed (545 vs. 480 g/day, P=0.22). The maximal growth rate observed for Mangalitsa was 690 g and for Moravka 607 g in the period corresponding to average body weight (end of period) of 79 and 89 kg, respectively. Initial (at start of the study) difference in growth rate between Mangalitsa and Moravka was propagated along the study duration resulting in significant difference in body weight at slaughter (P (lt) 0.05). © 2017, University of Zagreb. All rights reserved.",
journal = "Agriculturae Conspectus Scientificus",
title = "Growth potential of Serbian local pig breeds Mangalitsa and Moravka",
volume = "82",
number = "3 Special Issue 2",
pages = "217-220",
doi = "10.5281/zenodo.1136193"
}

Radović, Č., Petrović, M., Savić, R., Gogić, M., Lukić, M., Stanišić, N.,& Čandek-Potokar, M.. (2017). Growth potential of Serbian local pig breeds Mangalitsa and Moravka. in Agriculturae Conspectus Scientificus, 82(3 Special Issue 2), 217-220.
https://doi.org/10.5281/zenodo.1136193

Radović Č, Petrović M, Savić R, Gogić M, Lukić M, Stanišić N, Čandek-Potokar M. Growth potential of Serbian local pig breeds Mangalitsa and Moravka. in Agriculturae Conspectus Scientificus. 2017;82(3 Special Issue 2):217-220.
doi:10.5281/zenodo.1136193 .

Radović, Čedomir, Petrović, M., Savić, Radomir, Gogić, Marija, Lukić, Miloš, Stanišić, Nikola, Čandek-Potokar, Marjeta, "Growth potential of Serbian local pig breeds Mangalitsa and Moravka" in Agriculturae Conspectus Scientificus, 82, no. 3 Special Issue 2 (2017):217-220,
https://doi.org/10.5281/zenodo.1136193 . .

TY  - CONF
AU  - Óvilo, Cristina
AU  - Bozzi, Riccardo
AU  - García, F.
AU  - Nuñez, Yolanda
AU  - Geraci, Claudia
AU  - Crovetti, Alessandro
AU  - Garcia Casco, Juan Maria
AU  - Alves, Estefânia
AU  - Škrlep, Martin
AU  - Charneca, Rui
AU  - Quintanilla, Raquel
AU  - Kušec, Goran
AU  - Riquet, Juliette
AU  - Mercat, Marie-José
AU  - Zimmer, Christoph
AU  - Razmaitė, Violeta
AU  - Araujo, José Pedro
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Čandek-Potokar, Marjeta
AU  - Fontanesi, Luca
AU  - Fernández, Ana
AU  - Muñoz, Maria
PY  - 2017
UR  - https://zenodo.org/record/1135160
UR  - http://epserbia.open.ac.rs/123456789/196
UR  - http://r.istocar.bg.ac.rs/handle/123456789/572
AB  - In the frame of the European TREASURE* project, the genetic characterization of 20 local pig breeds is being performed with genetic and genomic tools. The objectives are the study of genetic diversity in these populations and the identification of useful markers for authentication, traceability, conservation and breeding programs. In first place, a candidate gene approach has been applied and the most relevant genes and mutations associated with pig productive, meat quality, reproductive and disease resistance traits have  been prioritized and analyzed. Two OpenArray® chips of 32 and 16 SNPs were designed and genotyped in 48 animals from each one of 20 breeds included in the project (Alentejana, Apulo Calabrese, Basque, Bísaro, Black Majorcan, Black Sicilian, Black Slavonian, Casertana, Cinta Senese, Gascon, Iberian, Krskopolje, Lithuanian indigenous wattle, Mangalitsa, Mora Romagnola, Moravka, Old Lithuanian White, Sarda, Schwäbisch Hällisches, Turopolje). Forty SNPs located in 34 genes were successfully genotyped (MC1R, TYRP1, NR6A, PCK1, RYR1, IGF2, MC4R, PHKG1, SCD, GBP5, TAS2R39, TAS2R4, MUC4, ESR1, CYP2E1, LEP, CAST, MTTP, CYB5A, FTO, PPARGC1A, CAPN1, PPARD, CTSL, LEPR, PRKAG3, ACACA, KIT, ACSL4, ADIPOQ, FASN, AHR, FUT1, MSTN). Results provide relevant information regarding genetic diversity and segregation of SNPs responsible for specific production and quality traits. Coat color and morphological trait-genes, showing low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for breeding programs. For instance, we observed predominance of unfavorable alleles for disease resistance and boar taint genes in most breeds, and segregation of many genes involved in meat quality, fatness and growth. These results joint with ongoing genomic assays, will provide essential information regarding genetic diversity, structure, selective signatures and biological processes responsible for specific production and quality traits.
PB  - Badajoz : CICYTEX
C3  - Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
T1  - Diversity across Major and Candidate Genes in European Local Pig Breeds
SP  - 51
EP  - 52
DO  - 10.5281/zenodo.1135160
ER  - 

@conference{
author = "Óvilo, Cristina and Bozzi, Riccardo and García, F. and Nuñez, Yolanda and Geraci, Claudia and Crovetti, Alessandro and Garcia Casco, Juan Maria and Alves, Estefânia and Škrlep, Martin and Charneca, Rui and Quintanilla, Raquel and Kušec, Goran and Riquet, Juliette and Mercat, Marie-José and Zimmer, Christoph and Razmaitė, Violeta and Araujo, José Pedro and Radović, Čedomir and Savić, Radomir and Čandek-Potokar, Marjeta and Fontanesi, Luca and Fernández, Ana and Muñoz, Maria",
year = "2017",
abstract = "In the frame of the European TREASURE* project, the genetic characterization of 20 local pig breeds is being performed with genetic and genomic tools. The objectives are the study of genetic diversity in these populations and the identification of useful markers for authentication, traceability, conservation and breeding programs. In first place, a candidate gene approach has been applied and the most relevant genes and mutations associated with pig productive, meat quality, reproductive and disease resistance traits have  been prioritized and analyzed. Two OpenArray® chips of 32 and 16 SNPs were designed and genotyped in 48 animals from each one of 20 breeds included in the project (Alentejana, Apulo Calabrese, Basque, Bísaro, Black Majorcan, Black Sicilian, Black Slavonian, Casertana, Cinta Senese, Gascon, Iberian, Krskopolje, Lithuanian indigenous wattle, Mangalitsa, Mora Romagnola, Moravka, Old Lithuanian White, Sarda, Schwäbisch Hällisches, Turopolje). Forty SNPs located in 34 genes were successfully genotyped (MC1R, TYRP1, NR6A, PCK1, RYR1, IGF2, MC4R, PHKG1, SCD, GBP5, TAS2R39, TAS2R4, MUC4, ESR1, CYP2E1, LEP, CAST, MTTP, CYB5A, FTO, PPARGC1A, CAPN1, PPARD, CTSL, LEPR, PRKAG3, ACACA, KIT, ACSL4, ADIPOQ, FASN, AHR, FUT1, MSTN). Results provide relevant information regarding genetic diversity and segregation of SNPs responsible for specific production and quality traits. Coat color and morphological trait-genes, showing low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for breeding programs. For instance, we observed predominance of unfavorable alleles for disease resistance and boar taint genes in most breeds, and segregation of many genes involved in meat quality, fatness and growth. These results joint with ongoing genomic assays, will provide essential information regarding genetic diversity, structure, selective signatures and biological processes responsible for specific production and quality traits.",
publisher = "Badajoz : CICYTEX",
journal = "Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference",
title = "Diversity across Major and Candidate Genes in European Local Pig Breeds",
pages = "51-52",
doi = "10.5281/zenodo.1135160"
}

Óvilo, C., Bozzi, R., García, F., Nuñez, Y., Geraci, C., Crovetti, A., Garcia Casco, J. M., Alves, E., Škrlep, M., Charneca, R., Quintanilla, R., Kušec, G., Riquet, J., Mercat, M., Zimmer, C., Razmaitė, V., Araujo, J. P., Radović, Č., Savić, R., Čandek-Potokar, M., Fontanesi, L., Fernández, A.,& Muñoz, M.. (2017). Diversity across Major and Candidate Genes in European Local Pig Breeds. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference
Badajoz : CICYTEX., 51-52.
https://doi.org/10.5281/zenodo.1135160

Óvilo C, Bozzi R, García F, Nuñez Y, Geraci C, Crovetti A, Garcia Casco JM, Alves E, Škrlep M, Charneca R, Quintanilla R, Kušec G, Riquet J, Mercat M, Zimmer C, Razmaitė V, Araujo JP, Radović Č, Savić R, Čandek-Potokar M, Fontanesi L, Fernández A, Muñoz M. Diversity across Major and Candidate Genes in European Local Pig Breeds. in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference. 2017;:51-52.
doi:10.5281/zenodo.1135160 .

Óvilo, Cristina, Bozzi, Riccardo, García, F., Nuñez, Yolanda, Geraci, Claudia, Crovetti, Alessandro, Garcia Casco, Juan Maria, Alves, Estefânia, Škrlep, Martin, Charneca, Rui, Quintanilla, Raquel, Kušec, Goran, Riquet, Juliette, Mercat, Marie-José, Zimmer, Christoph, Razmaitė, Violeta, Araujo, José Pedro, Radović, Čedomir, Savić, Radomir, Čandek-Potokar, Marjeta, Fontanesi, Luca, Fernández, Ana, Muñoz, Maria, "Diversity across Major and Candidate Genes in European Local Pig Breeds" in Book of Abstracts of the 4th Fatty Pig Science and Utilization International Conference (2017):51-52,
https://doi.org/10.5281/zenodo.1135160 . .