TY  - JOUR
AU  - Cekić, Bogdan
AU  - Kanzova, Hristiyana
AU  - Petrov, Georgi
AU  - Maksimović, Nevena
AU  - Ćosić, Ivan
AU  - Milovanović, Aleksandar
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/797
AB  - The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm.
AB  - Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
T2  - Biotechnology in animal husbandry
T1  - Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
VL  - 38
IS  - 1
SP  - 31
EP  - 39
DO  - 10.2298/BAH2201031C
ER  - 

@article{
author = "Cekić, Bogdan and Kanzova, Hristiyana and Petrov, Georgi and Maksimović, Nevena and Ćosić, Ivan and Milovanović, Aleksandar",
year = "2022",
abstract = "The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm., Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Biotechnology in animal husbandry",
title = "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams",
volume = "38",
number = "1",
pages = "31-39",
doi = "10.2298/BAH2201031C"
}

Cekić, B., Kanzova, H., Petrov, G., Maksimović, N., Ćosić, I.,& Milovanović, A.. (2022). Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 38(1), 31-39.
https://doi.org/10.2298/BAH2201031C

Cekić B, Kanzova H, Petrov G, Maksimović N, Ćosić I, Milovanović A. Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry. 2022;38(1):31-39.
doi:10.2298/BAH2201031C .

Cekić, Bogdan, Kanzova, Hristiyana, Petrov, Georgi, Maksimović, Nevena, Ćosić, Ivan, Milovanović, Aleksandar, "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams" in Biotechnology in animal husbandry, 38, no. 1 (2022):31-39,
https://doi.org/10.2298/BAH2201031C . .

TY  - CONF
AU  - Stojanov, Igor
AU  - Pušić, Ivan
AU  - Milovanović, Aleksandar
AU  - Ratajac, Radomir
AU  - Maksimović, Nevena
PY  - 2021-09
UR  - http://r.istocar.bg.ac.rs/handle/123456789/770
AB  - Anthelmintic resistance (AR) represents the reduced sensitivity of parasites to substances
used for the treatment of parasitosis of animals, ie, the possibility of their survival, to the recommended
doses of used antiparasitics used for certain types of parasites. The appearance of this
undesirable characteristic occurs as a consequence of inadequate use of antiparasitics during the
treatment of diseased animals as well as the need for better economic results in modern livestock
production. Available data indicate that AR has been recorded in many countries, and preliminary
studies show that it is also present in our country. Pasture farming allows the spread of AR in
nature and adverse effects on wildlife.
PB  - ECOLOGICAL MOVEMENT OF NOVI SAD
C3  - XXV International ECO-Conference: XIV Environmental protection of urban and suburban settlements, 22th–24th September, Novi Sad, Serbia
T1  - Antihelmintic resistance as an environmental issue
SP  - 328
EP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_770
ER  - 

@conference{
author = "Stojanov, Igor and Pušić, Ivan and Milovanović, Aleksandar and Ratajac, Radomir and Maksimović, Nevena",
year = "2021-09",
abstract = "Anthelmintic resistance (AR) represents the reduced sensitivity of parasites to substances
used for the treatment of parasitosis of animals, ie, the possibility of their survival, to the recommended
doses of used antiparasitics used for certain types of parasites. The appearance of this
undesirable characteristic occurs as a consequence of inadequate use of antiparasitics during the
treatment of diseased animals as well as the need for better economic results in modern livestock
production. Available data indicate that AR has been recorded in many countries, and preliminary
studies show that it is also present in our country. Pasture farming allows the spread of AR in
nature and adverse effects on wildlife.",
publisher = "ECOLOGICAL MOVEMENT OF NOVI SAD",
journal = "XXV International ECO-Conference: XIV Environmental protection of urban and suburban settlements, 22th–24th September, Novi Sad, Serbia",
title = "Antihelmintic resistance as an environmental issue",
pages = "328-335",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_770"
}

Stojanov, I., Pušić, I., Milovanović, A., Ratajac, R.,& Maksimović, N.. (2021-09). Antihelmintic resistance as an environmental issue. in XXV International ECO-Conference: XIV Environmental protection of urban and suburban settlements, 22th–24th September, Novi Sad, Serbia
ECOLOGICAL MOVEMENT OF NOVI SAD., 328-335.
https://hdl.handle.net/21.15107/rcub_ristocar_770

Stojanov I, Pušić I, Milovanović A, Ratajac R, Maksimović N. Antihelmintic resistance as an environmental issue. in XXV International ECO-Conference: XIV Environmental protection of urban and suburban settlements, 22th–24th September, Novi Sad, Serbia. 2021;:328-335.
https://hdl.handle.net/21.15107/rcub_ristocar_770 .

Stojanov, Igor, Pušić, Ivan, Milovanović, Aleksandar, Ratajac, Radomir, Maksimović, Nevena, "Antihelmintic resistance as an environmental issue" in XXV International ECO-Conference: XIV Environmental protection of urban and suburban settlements, 22th–24th September, Novi Sad, Serbia (2021-09):328-335,
https://hdl.handle.net/21.15107/rcub_ristocar_770 .

TY  - JOUR
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Mašić, Aleksandar
AU  - Lazarević, Marina
AU  - Pavlović, Miloš
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/762
AB  - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome
PB  - TUBITAK
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Quality and fertility of extended boar semen after prolonged transport
VL  - 45
IS  - 5
SP  - 920
EP  - 929
DO  - 10.3906/vet-2012-63
ER  - 

@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Marina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome",
publisher = "TUBITAK",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/vet-2012-63"
}

Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
TUBITAK., 45(5), 920-929.
https://doi.org/10.3906/vet-2012-63

Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/vet-2012-63 .

Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Marina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/vet-2012-63 . .

TY  - JOUR
AU  - Maksimović, Nevena
AU  - Hristov, Slavča
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stojanov, Igor
AU  - Cekić, Bogdan
AU  - Milošević-Stanković, Ivana
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/760
AB  - The drive to mate females is variable among the rams and can have a major impact on sheep production. Androgen testosterone
may be a key mediator within the expression of various morphological and behavioural traits in mammals, but the factors underlying
individual variation in circulating testosterone levels are poorly understood.
The aim of this study was to investigate the development of sexual behaviour patterns in ram lambs as well as the role of testosterone
in the expression of their libido.
Research was carried out on the sheep farm of the Institute for Animal Husbandry, Belgrade, Serbia. The study included 20 crossbred
ram lambs (autochthonous breed Pirot Pramenka x Merinolandschaf x Ile de France). All ram lambs used in the study were
of same age and rearing conditions. They were weaned at 2 months of age and kept indoors from birth throughout the whole
study. Animals were introduced in the study at the age of 3 months and the trial was completed at the rams’ age of 17 months.
Blood samples for testosterone levels were taken bimonthly (at the age of 3, 5, 7, 9, 11, 13, 15 and 17 months), as well as were
male-female and male-male interactions recorded.
Male-female interactions included the following elements of behaviour: nosing (or anogenital sniffing), pawing, flehmen response,
attempted mounts. Also, duration of all male activities directed towards ewe was recorded (male-female interactions in total).
Male-male interactions involved the frequency of male-male mounts.
Performed investigations showed that sexual behaviour of rams was age dependent, but poorly correlated to serum testosterone.
The average serum testosterone levels ranged from 1.83-13.28 ng/mL, and were age dependant (P<0.05).
Male-female oriented behaviour developed linearly with age, while male-male specific behaviour was characterized by high intensity
in young age and then pronounced variability in later test periods. None of the studied behavioural interactions were highly
correlated to serum testosterone. These findings support standpoint of more than one factor influencing development of sexual
behaviour of ram lambs.
PB  - SIVAR (Italian Society of Farm Animals Veterinary Practitioners)
T2  - Large animal review
T1  - Development of sexual behaviour in ram lambs and its correlation to serum testosterone
VL  - 27
SP  - 31
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_760
ER  - 

@article{
author = "Maksimović, Nevena and Hristov, Slavča and Milovanović, Aleksandar and Barna, Tomislav and Stojanov, Igor and Cekić, Bogdan and Milošević-Stanković, Ivana",
year = "2021",
abstract = "The drive to mate females is variable among the rams and can have a major impact on sheep production. Androgen testosterone
may be a key mediator within the expression of various morphological and behavioural traits in mammals, but the factors underlying
individual variation in circulating testosterone levels are poorly understood.
The aim of this study was to investigate the development of sexual behaviour patterns in ram lambs as well as the role of testosterone
in the expression of their libido.
Research was carried out on the sheep farm of the Institute for Animal Husbandry, Belgrade, Serbia. The study included 20 crossbred
ram lambs (autochthonous breed Pirot Pramenka x Merinolandschaf x Ile de France). All ram lambs used in the study were
of same age and rearing conditions. They were weaned at 2 months of age and kept indoors from birth throughout the whole
study. Animals were introduced in the study at the age of 3 months and the trial was completed at the rams’ age of 17 months.
Blood samples for testosterone levels were taken bimonthly (at the age of 3, 5, 7, 9, 11, 13, 15 and 17 months), as well as were
male-female and male-male interactions recorded.
Male-female interactions included the following elements of behaviour: nosing (or anogenital sniffing), pawing, flehmen response,
attempted mounts. Also, duration of all male activities directed towards ewe was recorded (male-female interactions in total).
Male-male interactions involved the frequency of male-male mounts.
Performed investigations showed that sexual behaviour of rams was age dependent, but poorly correlated to serum testosterone.
The average serum testosterone levels ranged from 1.83-13.28 ng/mL, and were age dependant (P<0.05).
Male-female oriented behaviour developed linearly with age, while male-male specific behaviour was characterized by high intensity
in young age and then pronounced variability in later test periods. None of the studied behavioural interactions were highly
correlated to serum testosterone. These findings support standpoint of more than one factor influencing development of sexual
behaviour of ram lambs.",
publisher = "SIVAR (Italian Society of Farm Animals Veterinary Practitioners)",
journal = "Large animal review",
title = "Development of sexual behaviour in ram lambs and its correlation to serum testosterone",
volume = "27",
pages = "31-34",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_760"
}

Maksimović, N., Hristov, S., Milovanović, A., Barna, T., Stojanov, I., Cekić, B.,& Milošević-Stanković, I.. (2021). Development of sexual behaviour in ram lambs and its correlation to serum testosterone. in Large animal review
SIVAR (Italian Society of Farm Animals Veterinary Practitioners)., 27, 31-34.
https://hdl.handle.net/21.15107/rcub_ristocar_760

Maksimović N, Hristov S, Milovanović A, Barna T, Stojanov I, Cekić B, Milošević-Stanković I. Development of sexual behaviour in ram lambs and its correlation to serum testosterone. in Large animal review. 2021;27:31-34.
https://hdl.handle.net/21.15107/rcub_ristocar_760 .

Maksimović, Nevena, Hristov, Slavča, Milovanović, Aleksandar, Barna, Tomislav, Stojanov, Igor, Cekić, Bogdan, Milošević-Stanković, Ivana, "Development of sexual behaviour in ram lambs and its correlation to serum testosterone" in Large animal review, 27 (2021):31-34,
https://hdl.handle.net/21.15107/rcub_ristocar_760 .

TY  - CONF
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Barna, Tomislav
AU  - Živkov-Baloš, Milica
AU  - Prodanov Radulović, Jasna
AU  - Jakšić, Sandra
AU  - Maksimović, Nevena
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/710
AB  - This paper describes escalation of sudden drop in semen quality in two boars' farm centers
affected by high mycotoxin level in forage (primarily by deoxynivalenol-DON). Barley and
wheat harvested in 2019 was generally indicated as most risky grains for animal health and
production and main source of DON. DON dominantly affected sperm total and progressive
motility (agglutination and astenospermia) as well as sperm chromatin structure, while acrosome
status and semen morphology were less sensitive to these changes. Bacterial control indicated
increase of bacterial presence. After forage replacement a period of 1-2 months is essential for
recovery of semen production, supported with prolonged vitamins and antibiotic treatment.
Routine control of forage for high producing animals is essential. Sperm production is
affected with DON level when is even below recommended standards for sows feed (cumulative
effect), and no clinical signs may appear. Commercial mycotoxin absorbers are not efficient for
such production.
PB  - Ecological Movement of the City of Novi Sad
C3  - Proceedings of XXIV International Eco-Conference on Safe Food
T1  - Effect of mycotoxins on porcine semen quality in artificial insemination centers
SP  - 271
EP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_710
ER  - 

@conference{
author = "Milovanović, Aleksandar and Apić, Jelena and Barna, Tomislav and Živkov-Baloš, Milica and Prodanov Radulović, Jasna and Jakšić, Sandra and Maksimović, Nevena",
year = "2020",
abstract = "This paper describes escalation of sudden drop in semen quality in two boars' farm centers
affected by high mycotoxin level in forage (primarily by deoxynivalenol-DON). Barley and
wheat harvested in 2019 was generally indicated as most risky grains for animal health and
production and main source of DON. DON dominantly affected sperm total and progressive
motility (agglutination and astenospermia) as well as sperm chromatin structure, while acrosome
status and semen morphology were less sensitive to these changes. Bacterial control indicated
increase of bacterial presence. After forage replacement a period of 1-2 months is essential for
recovery of semen production, supported with prolonged vitamins and antibiotic treatment.
Routine control of forage for high producing animals is essential. Sperm production is
affected with DON level when is even below recommended standards for sows feed (cumulative
effect), and no clinical signs may appear. Commercial mycotoxin absorbers are not efficient for
such production.",
publisher = "Ecological Movement of the City of Novi Sad",
journal = "Proceedings of XXIV International Eco-Conference on Safe Food",
title = "Effect of mycotoxins on porcine semen quality in artificial insemination centers",
pages = "271-278",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_710"
}

Milovanović, A., Apić, J., Barna, T., Živkov-Baloš, M., Prodanov Radulović, J., Jakšić, S.,& Maksimović, N.. (2020). Effect of mycotoxins on porcine semen quality in artificial insemination centers. in Proceedings of XXIV International Eco-Conference on Safe Food
Ecological Movement of the City of Novi Sad., 271-278.
https://hdl.handle.net/21.15107/rcub_ristocar_710

Milovanović A, Apić J, Barna T, Živkov-Baloš M, Prodanov Radulović J, Jakšić S, Maksimović N. Effect of mycotoxins on porcine semen quality in artificial insemination centers. in Proceedings of XXIV International Eco-Conference on Safe Food. 2020;:271-278.
https://hdl.handle.net/21.15107/rcub_ristocar_710 .

Milovanović, Aleksandar, Apić, Jelena, Barna, Tomislav, Živkov-Baloš, Milica, Prodanov Radulović, Jasna, Jakšić, Sandra, Maksimović, Nevena, "Effect of mycotoxins on porcine semen quality in artificial insemination centers" in Proceedings of XXIV International Eco-Conference on Safe Food (2020):271-278,
https://hdl.handle.net/21.15107/rcub_ristocar_710 .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Prodanov Radulović, Jasna
AU  - Apić, Jelena
AU  - Stojanović, Dragica
AU  - Maksimović, Nevena
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/708
AB  - The aim of the study was to determine whether the bacteria from the environment and
from the mucous membrane of the boar prepuce have antimicrobial resistance and
whether the result obtained is similar/same to the bacteria that can be found in native
boar semen. The study addresses the problem of the presence of primarily resistant
bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be
suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the
importance of microbiological monitoring of the boar mucous membranes and ambient
surfaces before and during their exploitation. Such an examination could contribute to
the interchangeable design of the dilution agent for the boar semen relative to the
antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken
from the facility, as well as from native boar semen were isolated. The presence of these
bacteria affected the quality of the semen. In conclusion, it should be pointed out that
bacterial monitoring of the prepuce and surface of the facility can indicate possible
problems related to the quality of semen, and that the design of the dilution agent for
boar semen should be adjusted to the established resistance of isolated bacteria.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Antimicrobial resistance as a problem for the quality of boar semen
VL  - 7
IS  - 1
SP  - 136
EP  - 146
DO  - 10.2478/acve-2020-0010
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Barna, Tomislav and Prodanov Radulović, Jasna and Apić, Jelena and Stojanović, Dragica and Maksimović, Nevena",
year = "2020",
abstract = "The aim of the study was to determine whether the bacteria from the environment and
from the mucous membrane of the boar prepuce have antimicrobial resistance and
whether the result obtained is similar/same to the bacteria that can be found in native
boar semen. The study addresses the problem of the presence of primarily resistant
bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be
suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the
importance of microbiological monitoring of the boar mucous membranes and ambient
surfaces before and during their exploitation. Such an examination could contribute to
the interchangeable design of the dilution agent for the boar semen relative to the
antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken
from the facility, as well as from native boar semen were isolated. The presence of these
bacteria affected the quality of the semen. In conclusion, it should be pointed out that
bacterial monitoring of the prepuce and surface of the facility can indicate possible
problems related to the quality of semen, and that the design of the dilution agent for
boar semen should be adjusted to the established resistance of isolated bacteria.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Antimicrobial resistance as a problem for the quality of boar semen",
volume = "7",
number = "1",
pages = "136-146",
doi = "10.2478/acve-2020-0010"
}

Stojanov, I., Milovanović, A., Barna, T., Prodanov Radulović, J., Apić, J., Stojanović, D.,& Maksimović, N.. (2020). Antimicrobial resistance as a problem for the quality of boar semen. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 7(1), 136-146.
https://doi.org/10.2478/acve-2020-0010

Stojanov I, Milovanović A, Barna T, Prodanov Radulović J, Apić J, Stojanović D, Maksimović N. Antimicrobial resistance as a problem for the quality of boar semen. in Acta Veterinaria-Beograd. 2020;7(1):136-146.
doi:10.2478/acve-2020-0010 .

Stojanov, Igor, Milovanović, Aleksandar, Barna, Tomislav, Prodanov Radulović, Jasna, Apić, Jelena, Stojanović, Dragica, Maksimović, Nevena, "Antimicrobial resistance as a problem for the quality of boar semen" in Acta Veterinaria-Beograd, 7, no. 1 (2020):136-146,
https://doi.org/10.2478/acve-2020-0010 . .

TY  - JOUR
AU  - Maksimović, Nevena
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Caro Petrović, Violeta
AU  - Pantelić, Vlada
AU  - Lazarević, Marina
AU  - Stojanov, Igor
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/554
AB  - The aim of this study was to investigate the effects of three extenders on ram sperm quality after short-term liquid storage (24 hours' holding time). The study included 20 crossbred rams (Pirot Pramenka x Wurttemberg x Ile de France), 12 months old. Animals were housed at the experimental sheep farm of the Institute for Animal Husbandry in Belgrade, Serbia. Semen was collected through electro ejaculation. The ejaculates were obtained from single services and routine field analysis of the semen was performed immediately after the collection. The semen was split and diluted with three extenders, namely Optidyl (R), Andromed (R) and ultrahigh temperature processed (UHT) milk, in ratios of 1 : 50 or 1 : 100. The ejaculates were examined for sperm motility variables (sperm cell motility percentage, the progressive motility percentage, curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), sperm linearity (LIN), straightness (STR), amplitude of lateral sperm head displacement (ALH), beat cross frequency (BCF) and circular tracks), and sperm morphology (live sperm percentage, percentage of normal sperm forms with intact acrosome, percentage of abnormal sperm forms and total damaged acrosome) by computer-assisted sperm analysis (CASA) and classic sperm cytology after supravital eosin/nigrosine/trypan blue staining, respectively. It was observed that the type of extender used in diluting ram semen is an important factor in the successful short-term liquid preservation (at 4 degrees C) of ram spermatozoa. In conclusion, this study showed that egg yolk (Optidyl) and soybean (Andromed)-based extenders gave better results of both sperm morphology and sperm motility parameters compared with UHT milk.
PB  - South African Journal Of Animal Sciences, Hatfield
T2  - South African Journal of Animal Science
T1  - Short-term liquid storage of ram semen in various extenders
VL  - 48
IS  - 4
SP  - 717
EP  - 723
DO  - 10.4314/sajas.v48i4.13
ER  - 

@article{
author = "Maksimović, Nevena and Milovanović, Aleksandar and Barna, Tomislav and Caro Petrović, Violeta and Pantelić, Vlada and Lazarević, Marina and Stojanov, Igor",
year = "2018",
abstract = "The aim of this study was to investigate the effects of three extenders on ram sperm quality after short-term liquid storage (24 hours' holding time). The study included 20 crossbred rams (Pirot Pramenka x Wurttemberg x Ile de France), 12 months old. Animals were housed at the experimental sheep farm of the Institute for Animal Husbandry in Belgrade, Serbia. Semen was collected through electro ejaculation. The ejaculates were obtained from single services and routine field analysis of the semen was performed immediately after the collection. The semen was split and diluted with three extenders, namely Optidyl (R), Andromed (R) and ultrahigh temperature processed (UHT) milk, in ratios of 1 : 50 or 1 : 100. The ejaculates were examined for sperm motility variables (sperm cell motility percentage, the progressive motility percentage, curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), sperm linearity (LIN), straightness (STR), amplitude of lateral sperm head displacement (ALH), beat cross frequency (BCF) and circular tracks), and sperm morphology (live sperm percentage, percentage of normal sperm forms with intact acrosome, percentage of abnormal sperm forms and total damaged acrosome) by computer-assisted sperm analysis (CASA) and classic sperm cytology after supravital eosin/nigrosine/trypan blue staining, respectively. It was observed that the type of extender used in diluting ram semen is an important factor in the successful short-term liquid preservation (at 4 degrees C) of ram spermatozoa. In conclusion, this study showed that egg yolk (Optidyl) and soybean (Andromed)-based extenders gave better results of both sperm morphology and sperm motility parameters compared with UHT milk.",
publisher = "South African Journal Of Animal Sciences, Hatfield",
journal = "South African Journal of Animal Science",
title = "Short-term liquid storage of ram semen in various extenders",
volume = "48",
number = "4",
pages = "717-723",
doi = "10.4314/sajas.v48i4.13"
}

Maksimović, N., Milovanović, A., Barna, T., Caro Petrović, V., Pantelić, V., Lazarević, M.,& Stojanov, I.. (2018). Short-term liquid storage of ram semen in various extenders. in South African Journal of Animal Science
South African Journal Of Animal Sciences, Hatfield., 48(4), 717-723.
https://doi.org/10.4314/sajas.v48i4.13

Maksimović N, Milovanović A, Barna T, Caro Petrović V, Pantelić V, Lazarević M, Stojanov I. Short-term liquid storage of ram semen in various extenders. in South African Journal of Animal Science. 2018;48(4):717-723.
doi:10.4314/sajas.v48i4.13 .

Maksimović, Nevena, Milovanović, Aleksandar, Barna, Tomislav, Caro Petrović, Violeta, Pantelić, Vlada, Lazarević, Marina, Stojanov, Igor, "Short-term liquid storage of ram semen in various extenders" in South African Journal of Animal Science, 48, no. 4 (2018):717-723,
https://doi.org/10.4314/sajas.v48i4.13 . .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Ratajac, Radomir
AU  - Živkov-Baloš, Milica
AU  - Maksimović, Nevena
AU  - Apić, Jelena
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/556
AB  - The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. The aim of the research was to determine whether the combination of EDTA-Tris and CHX in concentrations that are not harmful to the endometrium may result in the satisfactory treatment of sick animals and provide optimal service periods of reproductively active animals. Research results of in vitro and in vivo analysis showed that by using the combination of EDTA-Tris and CHX bactericidal effects were achieved at lower concentrations in comparison with the concentrations that had the same effect in the absence of EDTA-Tris. The application of CHX in a concentration of 0.05% in the treatment of endometritis 10 min after infusion of EDTA-Tris solution had a positive effect on both the involution of the uterus and acutization of chronic inflammatory processes, and it was also economically advantageous when compared to the cost of treatment and the possible presence of residues in the products.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows
VL  - 42
IS  - 1
SP  - 91
EP  - 96
DO  - 10.3906/vet-1703-74
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Ružić-Muslić, Dragana and Ratajac, Radomir and Živkov-Baloš, Milica and Maksimović, Nevena and Apić, Jelena",
year = "2018",
abstract = "The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. The aim of the research was to determine whether the combination of EDTA-Tris and CHX in concentrations that are not harmful to the endometrium may result in the satisfactory treatment of sick animals and provide optimal service periods of reproductively active animals. Research results of in vitro and in vivo analysis showed that by using the combination of EDTA-Tris and CHX bactericidal effects were achieved at lower concentrations in comparison with the concentrations that had the same effect in the absence of EDTA-Tris. The application of CHX in a concentration of 0.05% in the treatment of endometritis 10 min after infusion of EDTA-Tris solution had a positive effect on both the involution of the uterus and acutization of chronic inflammatory processes, and it was also economically advantageous when compared to the cost of treatment and the possible presence of residues in the products.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows",
volume = "42",
number = "1",
pages = "91-96",
doi = "10.3906/vet-1703-74"
}

Stojanov, I., Milovanović, A., Ružić-Muslić, D., Ratajac, R., Živkov-Baloš, M., Maksimović, N.,& Apić, J.. (2018). The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 42(1), 91-96.
https://doi.org/10.3906/vet-1703-74

Stojanov I, Milovanović A, Ružić-Muslić D, Ratajac R, Živkov-Baloš M, Maksimović N, Apić J. The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows. in Turkish Journal of Veterinary & Animal Sciences. 2018;42(1):91-96.
doi:10.3906/vet-1703-74 .

Stojanov, Igor, Milovanović, Aleksandar, Ružić-Muslić, Dragana, Ratajac, Radomir, Živkov-Baloš, Milica, Maksimović, Nevena, Apić, Jelena, "The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows" in Turkish Journal of Veterinary & Animal Sciences, 42, no. 1 (2018):91-96,
https://doi.org/10.3906/vet-1703-74 . .

TY  - JOUR
AU  - Stefanov, Rossen
AU  - Chervenkov, Mihail
AU  - Anev, Georgi
AU  - Maksimović, Nevena
AU  - Andreeva, Madlena
AU  - Ivanova, Teodora
AU  - Milovanović, Aleksandar
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/565
AB  - Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemented as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (Na2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and pH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39˚S for 360 min. It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment.
AB  - Selen je element u tragovima, koji stimuliše antioksidantske odbrane i poboljšava reproduktivne funkcije kod ljudi i životinja, u obliku selenoproteina. Cilj studije je bio da se proceni efekat selena, dopunjenog u neorganskom ili organskom oblik u ishrani ovnova, na neke parametre semena. Eksperiment je obavljen sa 15 klinički zdravih ovnova severnoistočne bugarske merino rase. Životinje su podeljene u tri grupe (5 po grupi). Ovnovi prve eksperimentalne grupe (G1) dobijali su obrok sa dodatkom 4,0 mg natrijum selenita (Na2SeO3) po grlu dnevno, dok su životinje druge eksperimentalne grupe (G2) hranjene obrokom sa 1,83 g L-selenometionina (Sel- Plek, Alltech, SAD) po grlu dnevno. Na kraju, svaka životinja iz G1 i G2 dobijala je 1,83g selena dnevno. Kontrolna grupa (GC) dobila je obrok bez dodatka selena. Glavni sastav obroka u svakoj grupi bio je isti. Ejakulati su dobijeni preko veštačke vagine. Ocenjivani su sledeći parametri: volumen i pH ejakulata i pokretljivosti, koncentracija i preživljavanje in vitro spermatozoida na 39°C tokom 360 min. Utvrđeno je da dodatak obroku neorganskog i organskog selena doveo do povećanja zapremine ejakulata, pokretljivosti i preživljavanja spermatozoida. Na sveže dobijeno seme nije uticao tretman sa selenom.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams
T1  - Uticaj dodavanja neorganskog i organskog selena na kvalitet i količinu sperme ovnova severoistočne bugarske merino rase
VL  - 34
IS  - 1
SP  - 69
EP  - 81
DO  - 10.2298/bah1801069S
ER  - 

@article{
author = "Stefanov, Rossen and Chervenkov, Mihail and Anev, Georgi and Maksimović, Nevena and Andreeva, Madlena and Ivanova, Teodora and Milovanović, Aleksandar",
year = "2018",
abstract = "Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemented as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (Na2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and pH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39˚S for 360 min. It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment., Selen je element u tragovima, koji stimuliše antioksidantske odbrane i poboljšava reproduktivne funkcije kod ljudi i životinja, u obliku selenoproteina. Cilj studije je bio da se proceni efekat selena, dopunjenog u neorganskom ili organskom oblik u ishrani ovnova, na neke parametre semena. Eksperiment je obavljen sa 15 klinički zdravih ovnova severnoistočne bugarske merino rase. Životinje su podeljene u tri grupe (5 po grupi). Ovnovi prve eksperimentalne grupe (G1) dobijali su obrok sa dodatkom 4,0 mg natrijum selenita (Na2SeO3) po grlu dnevno, dok su životinje druge eksperimentalne grupe (G2) hranjene obrokom sa 1,83 g L-selenometionina (Sel- Plek, Alltech, SAD) po grlu dnevno. Na kraju, svaka životinja iz G1 i G2 dobijala je 1,83g selena dnevno. Kontrolna grupa (GC) dobila je obrok bez dodatka selena. Glavni sastav obroka u svakoj grupi bio je isti. Ejakulati su dobijeni preko veštačke vagine. Ocenjivani su sledeći parametri: volumen i pH ejakulata i pokretljivosti, koncentracija i preživljavanje in vitro spermatozoida na 39°C tokom 360 min. Utvrđeno je da dodatak obroku neorganskog i organskog selena doveo do povećanja zapremine ejakulata, pokretljivosti i preživljavanja spermatozoida. Na sveže dobijeno seme nije uticao tretman sa selenom.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams, Uticaj dodavanja neorganskog i organskog selena na kvalitet i količinu sperme ovnova severoistočne bugarske merino rase",
volume = "34",
number = "1",
pages = "69-81",
doi = "10.2298/bah1801069S"
}

Stefanov, R., Chervenkov, M., Anev, G., Maksimović, N., Andreeva, M., Ivanova, T.,& Milovanović, A.. (2018). Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 34(1), 69-81.
https://doi.org/10.2298/bah1801069S

Stefanov R, Chervenkov M, Anev G, Maksimović N, Andreeva M, Ivanova T, Milovanović A. Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams. in Biotechnology in Animal Husbandry. 2018;34(1):69-81.
doi:10.2298/bah1801069S .

Stefanov, Rossen, Chervenkov, Mihail, Anev, Georgi, Maksimović, Nevena, Andreeva, Madlena, Ivanova, Teodora, Milovanović, Aleksandar, "Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams" in Biotechnology in Animal Husbandry, 34, no. 1 (2018):69-81,
https://doi.org/10.2298/bah1801069S . .

TY  - GEN
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Ratajac, Radomir
AU  - Živkov Baloš, Milica
AU  - Maksimović, Nevena
AU  - Apić, Jelena
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/851
AB  - Savremeni pristup u stočarskoj proizvodnji mora da obuhvati kreiranje tehnoloških postupaka koji omogućavaju dobijanje proizvoda koji zadovaljavaju principe ekonomičnosti, kao i potrebu obzebeđivanja biološki vredne i bezbedne hrane. Poseban zadatak nauke je prepoznavanje nedostataka u postojećim rešenjima i dizajniranje novih postupaka koji će uspostavljanjem novih modela omogućiti ostvarenje postavljenih ciljeva. Sa obzirom, da tehnološki postupci, kroz vreme, divergiraju jer se rasčlanjivanjem pojedinih faza proizvodnje lakše uočavaju potencijalni problemi, može se reči da se iz istih razloga javlja i njihovo usložnjavanje, što uglavnom zavisi od stepena razvoja zemlje. Međutim, neke pojave, kao razvoj i širenje antimikrobne rezistencije, prisutne su u svim zemljama gde stočarstvo ima važnu ulogu u prehrambenoj industriji. Pored spomenute činjenice, potreba rešavanja nekih zdravstvenih poremećaja, kao što su reproduktivni problemi, zahtevaju iznalaženje novih postupaka lečenja.
U našoj zemlji postupak lečenja pojedinih formi reproduktivnih poremećaja zasniva se na upotrebi dostupnih antibiotika ili antiseptičnih sredstava. Često se pristupa upotrebi ovih preparata bez valjane kliničke i laboratorijske analize. Ovakav pristup terapiji sa jedne strane doprinosi pojavi antimikrobne rezistencije zbog korišćenja antibiotika i onda kada to nije opravdano, dok sa druge strane upotreba antiseptika, posebno u visokim koncetracijam, dovodi do ozlede endometrijuma i prolongiranja servis perioda. Pojava rezistencije dovodi do neželjenih konsekvenci kako u veterinarskoj tako i humanoj medici jer se gubi glavno oružije u borbi protiv mikroorganizama i sve češće ostaje bez opcije pri izboru antimikrobnog leka. Prolongiranje servis perioda kod krava dovodi do materijalnih gubitaka jer se gubi mogućnost dobijanja jednog teleta godišenje, a u težim slučajevima, često se gubi i sama krava.
Rezultati ovog istraživanja u in vitro uslovima, su pokazali da se primenom kombnacije EDTA-Tris i hlorheksidin glukunata, postiže baktericidni efekat u nižim koncetracijama u poređenju sa koncetracijama koje su imale isti efekat bez prisustva EDTA-Tris. Kod svih ispitujućih sojeva, posle izlaganja bakterijskih suspenzija delovanju EDTA-Tris, koji kao helat menja permeablnost ćelijskog zida, potvrđeno je znatno povećanje osetljivost na hlorheksidin što se manifestovalo smanjenjem potrebne koncetracije ovoga antiseptika koji je imao baktericidno dejstvo.
Primena hlorheksidin-glukonata u koncentraciji od 0,05% za tretman endometritisa 10 minuta nakon infuzije rastvora EDTA-Tris ima povoljno delovanje kako na involuciju materice, tako i na akutizaciju hroničnih upalnih procesa. Ovaj vid terapije u kliničkim ogledu (postizanje steonosti kao krajni cilj) ima komparativne prednosti u odnosu na postojeće tretmane u povoljnoj ceni preparata (oko 220 dinara - 1,78 EU centi po ispiranju), nema karencu za mleko i meso a oporavak tokom involucije nije značajnije narušen. Efekat kod krava sa hroničnim endometritisima je u rangu standardnog uspeha terapije postojećim terapeuticima, ili bolji, u zavisnosti od stepena upalnog procesa.
Implementacija dobijenih rezultata u ovom tehničkom rešenju doprinosi rešavanju važnog segmetna u stočarskoj proizvodnji vezanog za reprodukciju. Mogućnost korišćenja optimalnih reproduktivnih sposobnosti i obezbeđivanje dobijajna jednog teleta godišnje omogućava ekonomičnost proizvodnje. Pored toga upotreba antibiotika se smanjuje čime se direktno utiče na smanjenje pojave i širenja mikrobne rezistencije u stočarstvu a indirektno i u humanoj medicini.
T1  - Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_851
ER  - 

@misc{
author = "Stojanov, Igor and Milovanović, Aleksandar and Ružić-Muslić, Dragana and Ratajac, Radomir and Živkov Baloš, Milica and Maksimović, Nevena and Apić, Jelena",
year = "2017",
abstract = "Savremeni pristup u stočarskoj proizvodnji mora da obuhvati kreiranje tehnoloških postupaka koji omogućavaju dobijanje proizvoda koji zadovaljavaju principe ekonomičnosti, kao i potrebu obzebeđivanja biološki vredne i bezbedne hrane. Poseban zadatak nauke je prepoznavanje nedostataka u postojećim rešenjima i dizajniranje novih postupaka koji će uspostavljanjem novih modela omogućiti ostvarenje postavljenih ciljeva. Sa obzirom, da tehnološki postupci, kroz vreme, divergiraju jer se rasčlanjivanjem pojedinih faza proizvodnje lakše uočavaju potencijalni problemi, može se reči da se iz istih razloga javlja i njihovo usložnjavanje, što uglavnom zavisi od stepena razvoja zemlje. Međutim, neke pojave, kao razvoj i širenje antimikrobne rezistencije, prisutne su u svim zemljama gde stočarstvo ima važnu ulogu u prehrambenoj industriji. Pored spomenute činjenice, potreba rešavanja nekih zdravstvenih poremećaja, kao što su reproduktivni problemi, zahtevaju iznalaženje novih postupaka lečenja.
U našoj zemlji postupak lečenja pojedinih formi reproduktivnih poremećaja zasniva se na upotrebi dostupnih antibiotika ili antiseptičnih sredstava. Često se pristupa upotrebi ovih preparata bez valjane kliničke i laboratorijske analize. Ovakav pristup terapiji sa jedne strane doprinosi pojavi antimikrobne rezistencije zbog korišćenja antibiotika i onda kada to nije opravdano, dok sa druge strane upotreba antiseptika, posebno u visokim koncetracijam, dovodi do ozlede endometrijuma i prolongiranja servis perioda. Pojava rezistencije dovodi do neželjenih konsekvenci kako u veterinarskoj tako i humanoj medici jer se gubi glavno oružije u borbi protiv mikroorganizama i sve češće ostaje bez opcije pri izboru antimikrobnog leka. Prolongiranje servis perioda kod krava dovodi do materijalnih gubitaka jer se gubi mogućnost dobijanja jednog teleta godišenje, a u težim slučajevima, često se gubi i sama krava.
Rezultati ovog istraživanja u in vitro uslovima, su pokazali da se primenom kombnacije EDTA-Tris i hlorheksidin glukunata, postiže baktericidni efekat u nižim koncetracijama u poređenju sa koncetracijama koje su imale isti efekat bez prisustva EDTA-Tris. Kod svih ispitujućih sojeva, posle izlaganja bakterijskih suspenzija delovanju EDTA-Tris, koji kao helat menja permeablnost ćelijskog zida, potvrđeno je znatno povećanje osetljivost na hlorheksidin što se manifestovalo smanjenjem potrebne koncetracije ovoga antiseptika koji je imao baktericidno dejstvo.
Primena hlorheksidin-glukonata u koncentraciji od 0,05% za tretman endometritisa 10 minuta nakon infuzije rastvora EDTA-Tris ima povoljno delovanje kako na involuciju materice, tako i na akutizaciju hroničnih upalnih procesa. Ovaj vid terapije u kliničkim ogledu (postizanje steonosti kao krajni cilj) ima komparativne prednosti u odnosu na postojeće tretmane u povoljnoj ceni preparata (oko 220 dinara - 1,78 EU centi po ispiranju), nema karencu za mleko i meso a oporavak tokom involucije nije značajnije narušen. Efekat kod krava sa hroničnim endometritisima je u rangu standardnog uspeha terapije postojećim terapeuticima, ili bolji, u zavisnosti od stepena upalnog procesa.
Implementacija dobijenih rezultata u ovom tehničkom rešenju doprinosi rešavanju važnog segmetna u stočarskoj proizvodnji vezanog za reprodukciju. Mogućnost korišćenja optimalnih reproduktivnih sposobnosti i obezbeđivanje dobijajna jednog teleta godišnje omogućava ekonomičnost proizvodnje. Pored toga upotreba antibiotika se smanjuje čime se direktno utiče na smanjenje pojave i širenja mikrobne rezistencije u stočarstvu a indirektno i u humanoj medicini.",
title = "Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_851"
}

Stojanov, I., Milovanović, A., Ružić-Muslić, D., Ratajac, R., Živkov Baloš, M., Maksimović, N.,& Apić, J.. (2017). Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje. .
https://hdl.handle.net/21.15107/rcub_ristocar_851

Stojanov I, Milovanović A, Ružić-Muslić D, Ratajac R, Živkov Baloš M, Maksimović N, Apić J. Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje. 2017;.
https://hdl.handle.net/21.15107/rcub_ristocar_851 .

Stojanov, Igor, Milovanović, Aleksandar, Ružić-Muslić, Dragana, Ratajac, Radomir, Živkov Baloš, Milica, Maksimović, Nevena, Apić, Jelena, "Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje" (2017),
https://hdl.handle.net/21.15107/rcub_ristocar_851 .

TY  - JOUR
AU  - Maksimović, Nevena
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Delić, Nikola
AU  - Stefanov, Rossen
AU  - Pantelić, Vlada
AU  - Taushanova, Paulina
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/539
AB  - The study investigates the possibility of oestrus synchronization in ewes of MIS sheep population using prostaglandin (PG) outside the breeding season, serum progesterone concentration for pregnancy diagnosis and effect of hCG (human chorionic gonadotropin) on reproductive parameters. Prostaglandin was used to induce oestrus at the dose of 2.5 ml/ewe, administered intramuscularly in two injections (with 11 days interval). Responsive ewes were bred to rams and were divided into two groups, one of which was administered with hCG at the dose of 300 IU i/m 7th day post mating and the second group which was used as a control. Serum progesterone was measured on the day of prostaglandin administration and 17 days post mating. Oestrus response was 66.67%, lambing rate was 70%, average litter size was 1.7 lambs/ewe and twinning rate was 60%. Administration of hCG 7 days post mating did not improve fertility results. Lambing, prolificacy and twinning rates were higher in control than in hCG group, but no statistical difference was found between these groups. From a total of 20 ewes that were mated after PG treatment in 18 of them (90%), on the basis of progesterone concentration measured on the 17th day after mating and subsequent lambing results, gestation was confirmed or denied.
PB  - Publ House Bulgarian Acad Sci, Sofia
T2  - Comptes Rendus de l Academie Bulgare des Sciences
T1  - Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes
VL  - 70
IS  - 6
SP  - 885
EP  - 894
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_539
ER  - 

@article{
author = "Maksimović, Nevena and Milovanović, Aleksandar and Barna, Tomislav and Delić, Nikola and Stefanov, Rossen and Pantelić, Vlada and Taushanova, Paulina",
year = "2017",
abstract = "The study investigates the possibility of oestrus synchronization in ewes of MIS sheep population using prostaglandin (PG) outside the breeding season, serum progesterone concentration for pregnancy diagnosis and effect of hCG (human chorionic gonadotropin) on reproductive parameters. Prostaglandin was used to induce oestrus at the dose of 2.5 ml/ewe, administered intramuscularly in two injections (with 11 days interval). Responsive ewes were bred to rams and were divided into two groups, one of which was administered with hCG at the dose of 300 IU i/m 7th day post mating and the second group which was used as a control. Serum progesterone was measured on the day of prostaglandin administration and 17 days post mating. Oestrus response was 66.67%, lambing rate was 70%, average litter size was 1.7 lambs/ewe and twinning rate was 60%. Administration of hCG 7 days post mating did not improve fertility results. Lambing, prolificacy and twinning rates were higher in control than in hCG group, but no statistical difference was found between these groups. From a total of 20 ewes that were mated after PG treatment in 18 of them (90%), on the basis of progesterone concentration measured on the 17th day after mating and subsequent lambing results, gestation was confirmed or denied.",
publisher = "Publ House Bulgarian Acad Sci, Sofia",
journal = "Comptes Rendus de l Academie Bulgare des Sciences",
title = "Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes",
volume = "70",
number = "6",
pages = "885-894",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_539"
}

Maksimović, N., Milovanović, A., Barna, T., Delić, N., Stefanov, R., Pantelić, V.,& Taushanova, P.. (2017). Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes. in Comptes Rendus de l Academie Bulgare des Sciences
Publ House Bulgarian Acad Sci, Sofia., 70(6), 885-894.
https://hdl.handle.net/21.15107/rcub_ristocar_539

Maksimović N, Milovanović A, Barna T, Delić N, Stefanov R, Pantelić V, Taushanova P. Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes. in Comptes Rendus de l Academie Bulgare des Sciences. 2017;70(6):885-894.
https://hdl.handle.net/21.15107/rcub_ristocar_539 .

Maksimović, Nevena, Milovanović, Aleksandar, Barna, Tomislav, Delić, Nikola, Stefanov, Rossen, Pantelić, Vlada, Taushanova, Paulina, "Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes" in Comptes Rendus de l Academie Bulgare des Sciences, 70, no. 6 (2017):885-894,
https://hdl.handle.net/21.15107/rcub_ristocar_539 .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Barna, Tomislav
AU  - Lazarević, Marina
AU  - Delić, Nikola
PY  - 2013
UR  - http://r.istocar.bg.ac.rs/handle/123456789/401
AB  - This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.
AB  - U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Laparoscopic insemination of sheep in Republic of Serbia
T1  - Laparoskopsko osemenjavanje ovaca u Republici Srbiji
VL  - 29
IS  - 3
SP  - 449
EP  - 456
DO  - 10.2298/BAH1303449M
ER  - 

@article{
author = "Milovanović, Aleksandar and Maksimović, Nevena and Barna, Tomislav and Lazarević, Marina and Delić, Nikola",
year = "2013",
abstract = "This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks., U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Laparoscopic insemination of sheep in Republic of Serbia, Laparoskopsko osemenjavanje ovaca u Republici Srbiji",
volume = "29",
number = "3",
pages = "449-456",
doi = "10.2298/BAH1303449M"
}

Milovanović, A., Maksimović, N., Barna, T., Lazarević, M.,& Delić, N.. (2013). Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 29(3), 449-456.
https://doi.org/10.2298/BAH1303449M

Milovanović A, Maksimović N, Barna T, Lazarević M, Delić N. Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry. 2013;29(3):449-456.
doi:10.2298/BAH1303449M .

Milovanović, Aleksandar, Maksimović, Nevena, Barna, Tomislav, Lazarević, Marina, Delić, Nikola, "Laparoscopic insemination of sheep in Republic of Serbia" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):449-456,
https://doi.org/10.2298/BAH1303449M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Maksimović, Nevena
AU  - Vasiljević, T.
AU  - Milanov, D.
AU  - Bošković, N.
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/362
AB  - Serbia is one of the countries with the continuous import of breeding sows and boars. Boars are usually imported at the age of 4 to 6 months, in the period when the quality of the breeding males cannot be determined due to sexual immaturity (prepubertal and pubertal age). In this paper, the method and results of semen quality control in 40 imported young boars are described, and also the method of documenting the cause for action claim. In the case of suspicious semen quality it is necesseary to perform at least 3 consecutive controls in one month intervals in order to establish a final estimation of quality and usability of semen. Of 40 imported boars, 4 boars (10%) were subject of complaint due to: azoospermia (1 boar), absence or reduction of total and progressive motility, present sperm agglutination (2 boars), and increased number of pathological forms of spermatozoa (78%, 1 boar). Increased proportion of sperm with unstable chromatin structure (SCSA test - 33.2% and 37.1%) was established in two boars. To initiate the complaint it is necessary to have a sales contract that provides possibility for the reclamation, recognized methods of semen quality control and trustful business relationship between all interested parties.
AB  - Srbija se svrstava u red zemalja sa kontinuiranim uvozom priplodnih nazimica i nerastova. Nerastovi se uglavnom uvoze u dobi od 4 do 6 meseci, u periodu kada se kvalitet priplodnjaka ne može pouzdano utvrditi usled polne nezrelosti (prepubertetsko ili pubertetska dob nerastića). U ovom radu opisan je postupak i rezultati kontrole kvaliteta semena kod 40 mladih nerastova iz uvoza, kao i način dokumentovanja razloga za pokretanje postupka reklamacije. Kod sumnjivog kvaliteta semena potrebno je izvršiti najmanje 3 uzastopne kontrole u razmaku od po mesec dana, kako bi se donela konačna ocena o kvalitetu semena i upotrebljivosti nerasta za priplod. Od 40 uvezenih nerastova, 4 su reklamirana (10%) usled: azoospermije (1 nerast), odsustva ili smanjenje ukupne ili progresivne pokretljivosti, uz prisustvo aglutinacija spermatozoida (2 nerasta), i povećanog broja patoloških formi spermatozoida (78%; 1 nerast). Kod dva nerasta zabeležen je povećan udeo spermatozoida sa nestabilnom strukturom hromatina (SCSA test - 33,2% i 37,1%). Za pokretanje reklamacije neophodno je imati kupoprodajni ugovor koji predviđa mogućnost reklamacije, priznate metode kontrole semena kao i izgrađen poslovni odnos poverenja zainteresovanih strana.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Import of boars: Semen quality control and possibility of complaints
T1  - Uvoz nerastova - kontrola semena i mogućnost reklamacije
VL  - 28
IS  - 4
SP  - 759
EP  - 769
DO  - 10.2298/BAH1204759M
ER  - 

@article{
author = "Milovanović, Aleksandar and Barna, Tomislav and Maksimović, Nevena and Vasiljević, T. and Milanov, D. and Bošković, N.",
year = "2012",
abstract = "Serbia is one of the countries with the continuous import of breeding sows and boars. Boars are usually imported at the age of 4 to 6 months, in the period when the quality of the breeding males cannot be determined due to sexual immaturity (prepubertal and pubertal age). In this paper, the method and results of semen quality control in 40 imported young boars are described, and also the method of documenting the cause for action claim. In the case of suspicious semen quality it is necesseary to perform at least 3 consecutive controls in one month intervals in order to establish a final estimation of quality and usability of semen. Of 40 imported boars, 4 boars (10%) were subject of complaint due to: azoospermia (1 boar), absence or reduction of total and progressive motility, present sperm agglutination (2 boars), and increased number of pathological forms of spermatozoa (78%, 1 boar). Increased proportion of sperm with unstable chromatin structure (SCSA test - 33.2% and 37.1%) was established in two boars. To initiate the complaint it is necessary to have a sales contract that provides possibility for the reclamation, recognized methods of semen quality control and trustful business relationship between all interested parties., Srbija se svrstava u red zemalja sa kontinuiranim uvozom priplodnih nazimica i nerastova. Nerastovi se uglavnom uvoze u dobi od 4 do 6 meseci, u periodu kada se kvalitet priplodnjaka ne može pouzdano utvrditi usled polne nezrelosti (prepubertetsko ili pubertetska dob nerastića). U ovom radu opisan je postupak i rezultati kontrole kvaliteta semena kod 40 mladih nerastova iz uvoza, kao i način dokumentovanja razloga za pokretanje postupka reklamacije. Kod sumnjivog kvaliteta semena potrebno je izvršiti najmanje 3 uzastopne kontrole u razmaku od po mesec dana, kako bi se donela konačna ocena o kvalitetu semena i upotrebljivosti nerasta za priplod. Od 40 uvezenih nerastova, 4 su reklamirana (10%) usled: azoospermije (1 nerast), odsustva ili smanjenje ukupne ili progresivne pokretljivosti, uz prisustvo aglutinacija spermatozoida (2 nerasta), i povećanog broja patoloških formi spermatozoida (78%; 1 nerast). Kod dva nerasta zabeležen je povećan udeo spermatozoida sa nestabilnom strukturom hromatina (SCSA test - 33,2% i 37,1%). Za pokretanje reklamacije neophodno je imati kupoprodajni ugovor koji predviđa mogućnost reklamacije, priznate metode kontrole semena kao i izgrađen poslovni odnos poverenja zainteresovanih strana.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Import of boars: Semen quality control and possibility of complaints, Uvoz nerastova - kontrola semena i mogućnost reklamacije",
volume = "28",
number = "4",
pages = "759-769",
doi = "10.2298/BAH1204759M"
}

Milovanović, A., Barna, T., Maksimović, N., Vasiljević, T., Milanov, D.,& Bošković, N.. (2012). Import of boars: Semen quality control and possibility of complaints. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 28(4), 759-769.
https://doi.org/10.2298/BAH1204759M

Milovanović A, Barna T, Maksimović N, Vasiljević T, Milanov D, Bošković N. Import of boars: Semen quality control and possibility of complaints. in Biotechnology in Animal Husbandry. 2012;28(4):759-769.
doi:10.2298/BAH1204759M .

Milovanović, Aleksandar, Barna, Tomislav, Maksimović, Nevena, Vasiljević, T., Milanov, D., Bošković, N., "Import of boars: Semen quality control and possibility of complaints" in Biotechnology in Animal Husbandry, 28, no. 4 (2012):759-769,
https://doi.org/10.2298/BAH1204759M . .