TY  - JOUR
AU  - Tančić, Sonja L.
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2015
UR  - http://r.istocar.bg.ac.rs/handle/123456789/474
AB  - A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp.
AB  - Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.
PB  - Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain
T1  - Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza
VL  - 30
IS  - 2
SP  - 99
EP  - 105
DO  - 10.2298/pif1502099T
ER  - 

@article{
author = "Tančić, Sonja L. and Stanković, Slavica Ž. and Lević, Jelena and Krnjaja, Vesna",
year = "2015",
abstract = "A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp., Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain, Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza",
volume = "30",
number = "2",
pages = "99-105",
doi = "10.2298/pif1502099T"
}

Tančić, S. L., Stanković, S. Ž., Lević, J.,& Krnjaja, V.. (2015). Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade., 30(2), 99-105.
https://doi.org/10.2298/pif1502099T

Tančić SL, Stanković SŽ, Lević J, Krnjaja V. Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain. in Pesticidi i fitomedicina. 2015;30(2):99-105.
doi:10.2298/pif1502099T .

Tančić, Sonja L., Stanković, Slavica Ž., Lević, Jelena, Krnjaja, Vesna, "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain" in Pesticidi i fitomedicina, 30, no. 2 (2015):99-105,
https://doi.org/10.2298/pif1502099T . .

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Krnjaja, Vesna
AU  - Tančić, Sonja L.
AU  - Ivanović, Dragica
AU  - Bočarov-Stančić, Aleksandra S.
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/312
AB  - The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Relationships of mycobiota on rachides and kernels of wheat
VL  - 134
IS  - 2
SP  - 249
EP  - 256
DO  - 10.1007/s10658-012-9982-8
ER  - 

@article{
author = "Lević, Jelena and Stanković, Slavica Ž. and Krnjaja, Vesna and Tančić, Sonja L. and Ivanović, Dragica and Bočarov-Stančić, Aleksandra S.",
year = "2012",
abstract = "The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r = 0.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r = 0.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r = -0.3783 *) and kernels (r = -0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Relationships of mycobiota on rachides and kernels of wheat",
volume = "134",
number = "2",
pages = "249-256",
doi = "10.1007/s10658-012-9982-8"
}

Lević, J., Stanković, S. Ž., Krnjaja, V., Tančić, S. L., Ivanović, D.,& Bočarov-Stančić, A. S.. (2012). Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 249-256.
https://doi.org/10.1007/s10658-012-9982-8

Lević J, Stanković SŽ, Krnjaja V, Tančić SL, Ivanović D, Bočarov-Stančić AS. Relationships of mycobiota on rachides and kernels of wheat. in European Journal of Plant Pathology. 2012;134(2):249-256.
doi:10.1007/s10658-012-9982-8 .

Lević, Jelena, Stanković, Slavica Ž., Krnjaja, Vesna, Tančić, Sonja L., Ivanović, Dragica, Bočarov-Stančić, Aleksandra S., "Relationships of mycobiota on rachides and kernels of wheat" in European Journal of Plant Pathology, 134, no. 2 (2012):249-256,
https://doi.org/10.1007/s10658-012-9982-8 . .

TY  - JOUR
AU  - Tančić, Sonja L.
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Vukojević, Jelena
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/319
AB  - Species of the genus Fusarium are characterised by the exceptional intraspecies and interspecies variability in respect to morphological, physiological and genetic properties. Intraspecies and interspecies diversity of Fusarium verticillioides and Fusarium proliferatum isolates in the production of fumonisin B1 according to their origin from maize and wheat grains was studied. Fumonisin B1 production potential of investigated 42 isolates was assessed by HPTLC and ELISA method. All 22 and 20 investigated F. verticillioides and F. proliferatum isolates, respectively, had the ability to produce fumonisin B1 toxin. Fumonisin B1 production potential of F. verticillioides and F. proliferatum varied from 88.60 ppm to 1,300.60 ppm and from 2.37 ppm to 1,246.00 ppm, respectively. According to Mann-Whitnev U test, there were no significant differences between the fumonisin B1 production mean values of isolates of F. verticillioides originated from maize and wheat (628.13 ppm and 696.38 ppm, respectively), as well as, fumonisin B1 production mean values of F. proliferatum isolates (212.32 ppm and 158.07 ppm, respectively). Variability coefficient values indicated that fumonisin B1 production potential variability was more expressed in maize than wheat originated isolates for both species.
AB  - Vrste roda Fusarium karakteriše izuzetna intraspecijska i interspecijska varijabilnost u pogledu morfoloških, fizioloških i genetičkih svojstava. Proučavan je intraspecijski i interspecijski diverzitet izolata vrsta Fusarium verticillioides i Fusarium proliferatum u produkciji fumonizina B1 zavisno od njihovog porekla sa zrna kukuruza i pšenice. Potencijal sinteze fumonizina B1 kod 42 izolata je ispitivan HPTLC i ELISA metodom. Svih 22 izolata vrste F. verticillioides i 20 izolata vrste F. proliferatum su imali sposobnost stvaranja toksina fumonizina B1. Potencijal za sintezu fumonizina je varirao od 88.60 ppm do 1,300.60 ppm kod izolata vrste F. verticillioides i od 2.37 ppm do 1,246.00 ppm kod F. proliferatum izolata. Prema Mann-Whitnev U testu nije bilo statistički značajnih razlika srednjih vrednosti sintetisanog FB1 između F. verticillioides izolata poreklom sa kukuruza (628.13 ppm) i pšenice (696.38 ppm), kao ni kod F. proliferatum izolata poreklom sa kukuruza (212.32 ppm) i pšenice (158.07 ppm). Visoke vrednosti koeficijenta varijacije ukazuju da je varijabilnost u potencijalu sinteze fumonizina B1 bila više izražena kod izolata poreklom sa kukuruza nego pšenice kod obe ispitivane vrste.
PB  - Serbian Genetics Society, Belgrade
T2  - Genetika-Belgrade
T1  - Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin
T1  - Diverzitet Fusarium verticillioides i F. proliferatum izolata u potencijalu sinteze fumonizina B1 zavisno od porekla
VL  - 44
IS  - 1
SP  - 163
EP  - 176
DO  - 10.2298/GENSR1201163T
ER  - 

@article{
author = "Tančić, Sonja L. and Stanković, Slavica Ž. and Lević, Jelena and Krnjaja, Vesna and Vukojević, Jelena",
year = "2012",
abstract = "Species of the genus Fusarium are characterised by the exceptional intraspecies and interspecies variability in respect to morphological, physiological and genetic properties. Intraspecies and interspecies diversity of Fusarium verticillioides and Fusarium proliferatum isolates in the production of fumonisin B1 according to their origin from maize and wheat grains was studied. Fumonisin B1 production potential of investigated 42 isolates was assessed by HPTLC and ELISA method. All 22 and 20 investigated F. verticillioides and F. proliferatum isolates, respectively, had the ability to produce fumonisin B1 toxin. Fumonisin B1 production potential of F. verticillioides and F. proliferatum varied from 88.60 ppm to 1,300.60 ppm and from 2.37 ppm to 1,246.00 ppm, respectively. According to Mann-Whitnev U test, there were no significant differences between the fumonisin B1 production mean values of isolates of F. verticillioides originated from maize and wheat (628.13 ppm and 696.38 ppm, respectively), as well as, fumonisin B1 production mean values of F. proliferatum isolates (212.32 ppm and 158.07 ppm, respectively). Variability coefficient values indicated that fumonisin B1 production potential variability was more expressed in maize than wheat originated isolates for both species., Vrste roda Fusarium karakteriše izuzetna intraspecijska i interspecijska varijabilnost u pogledu morfoloških, fizioloških i genetičkih svojstava. Proučavan je intraspecijski i interspecijski diverzitet izolata vrsta Fusarium verticillioides i Fusarium proliferatum u produkciji fumonizina B1 zavisno od njihovog porekla sa zrna kukuruza i pšenice. Potencijal sinteze fumonizina B1 kod 42 izolata je ispitivan HPTLC i ELISA metodom. Svih 22 izolata vrste F. verticillioides i 20 izolata vrste F. proliferatum su imali sposobnost stvaranja toksina fumonizina B1. Potencijal za sintezu fumonizina je varirao od 88.60 ppm do 1,300.60 ppm kod izolata vrste F. verticillioides i od 2.37 ppm do 1,246.00 ppm kod F. proliferatum izolata. Prema Mann-Whitnev U testu nije bilo statistički značajnih razlika srednjih vrednosti sintetisanog FB1 između F. verticillioides izolata poreklom sa kukuruza (628.13 ppm) i pšenice (696.38 ppm), kao ni kod F. proliferatum izolata poreklom sa kukuruza (212.32 ppm) i pšenice (158.07 ppm). Visoke vrednosti koeficijenta varijacije ukazuju da je varijabilnost u potencijalu sinteze fumonizina B1 bila više izražena kod izolata poreklom sa kukuruza nego pšenice kod obe ispitivane vrste.",
publisher = "Serbian Genetics Society, Belgrade",
journal = "Genetika-Belgrade",
title = "Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin, Diverzitet Fusarium verticillioides i F. proliferatum izolata u potencijalu sinteze fumonizina B1 zavisno od porekla",
volume = "44",
number = "1",
pages = "163-176",
doi = "10.2298/GENSR1201163T"
}

Tančić, S. L., Stanković, S. Ž., Lević, J., Krnjaja, V.,& Vukojević, J.. (2012). Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin. in Genetika-Belgrade
Serbian Genetics Society, Belgrade., 44(1), 163-176.
https://doi.org/10.2298/GENSR1201163T

Tančić SL, Stanković SŽ, Lević J, Krnjaja V, Vukojević J. Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin. in Genetika-Belgrade. 2012;44(1):163-176.
doi:10.2298/GENSR1201163T .

Tančić, Sonja L., Stanković, Slavica Ž., Lević, Jelena, Krnjaja, Vesna, Vukojević, Jelena, "Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin" in Genetika-Belgrade, 44, no. 1 (2012):163-176,
https://doi.org/10.2298/GENSR1201163T . .

TY  - JOUR
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Ivanović, Dragica
AU  - Krnjaja, Vesna
AU  - Stanković, Goran
AU  - Tančić, Sonja L.
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/320
AB  - The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world. (C) 2011 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain
VL  - 23
IS  - 2
SP  - 384
EP  - 388
DO  - 10.1016/j.foodcont.2011.08.003
ER  - 

@article{
author = "Stanković, Slavica Ž. and Lević, Jelena and Ivanović, Dragica and Krnjaja, Vesna and Stanković, Goran and Tančić, Sonja L.",
year = "2012",
abstract = "The natural occurrence of fumonisin B-1 (FB1) and its co-occurrence with zearalenone (ZEA), T-2 toxin and deoxynivalenol (DON) were surveyed in 103 winter wheat samples collected after four to six-month storage in family barns from different locations in Serbia. All 103 samples were mycotoxin positive. The mean concentrations of all mycotoxins except ZEA were greater in 2005 than in 2007. FB1 was detected in 82.1% and 92.0% of all samples with ranges of 750-5400 mu g kg(-1) (mean, 2079.45 mu g kg(-1)) and 750-4900 mu g kg(-1) (mean 918.76 mu g kg(-1)) in 2005 and 2007, respectively. Moderate positive correlations were found between FB1 and DON concentrations (r = 0.56 in 2005 and r = 0.54 in 2007) and between FB1 and ZEA concentrations (r = 0.48 in 2005 and r = 0.60 in 2007), while a moderate negative correlation was detected between the production of FB1 and T-2 toxin in 2007 (r = -0.33). This is the first report of FB1 occurrence in naturally-contaminated wheat grain and its simultaneous occurrence with ZEA, DON and T-2 toxin in Serbia. Moreover, this is one of the rare reports presenting the occurrence of FB1 on wheat in the world. (C) 2011 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain",
volume = "23",
number = "2",
pages = "384-388",
doi = "10.1016/j.foodcont.2011.08.003"
}

Stanković, S. Ž., Lević, J., Ivanović, D., Krnjaja, V., Stanković, G.,& Tančić, S. L.. (2012). Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control
Elsevier Sci Ltd, Oxford., 23(2), 384-388.
https://doi.org/10.1016/j.foodcont.2011.08.003

Stanković SŽ, Lević J, Ivanović D, Krnjaja V, Stanković G, Tančić SL. Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain. in Food Control. 2012;23(2):384-388.
doi:10.1016/j.foodcont.2011.08.003 .

Stanković, Slavica Ž., Lević, Jelena, Ivanović, Dragica, Krnjaja, Vesna, Stanković, Goran, Tančić, Sonja L., "Fumonisin B-1 and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain" in Food Control, 23, no. 2 (2012):384-388,
https://doi.org/10.1016/j.foodcont.2011.08.003 . .

TY  - CONF
AU  - Bočarov-Stančić, Aleksandra S.
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Tančić, Sonja L.
AU  - Krnjaja, Vesna
AU  - Salma, N.
PY  - 2008
UR  - http://r.istocar.bg.ac.rs/handle/123456789/173
AB  - The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28C) with two different media: liquid GPYE and wett sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.312-12.0 ppm), but only one (MRIZP -1208) zearalenone.
PB  - Akademiai Kiado Rt, Budapest
C3  - Cereal Research Communications
T1  - Toxigenic potential of fusarium langsethiae isolates from Serbian wheat kernels
VL  - 36
IS  - SUPPL. 6
SP  - 345
EP  - 346
DO  - 10.1556/CRC.36.2008.Suppl.B.33
ER  - 

@conference{
author = "Bočarov-Stančić, Aleksandra S. and Lević, Jelena and Stanković, Slavica Ž. and Tančić, Sonja L. and Krnjaja, Vesna and Salma, N.",
year = "2008",
abstract = "The toxigenic potential of F. langsethiae cultures isolated from Serbian wheat kernels harvested in 2005 was investigated. In vitro experiments were performed at room temperature (24-28C) with two different media: liquid GPYE and wett sterilized wheat grain. All of the tested F. langsethiae isolates produced T-2 toxin (0.312 - 48.0 ppm) and DAS (0.312-12.0 ppm), but only one (MRIZP -1208) zearalenone.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Cereal Research Communications",
title = "Toxigenic potential of fusarium langsethiae isolates from Serbian wheat kernels",
volume = "36",
number = "SUPPL. 6",
pages = "345-346",
doi = "10.1556/CRC.36.2008.Suppl.B.33"
}

Bočarov-Stančić, A. S., Lević, J., Stanković, S. Ž., Tančić, S. L., Krnjaja, V.,& Salma, N.. (2008). Toxigenic potential of fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications
Akademiai Kiado Rt, Budapest., 36(SUPPL. 6), 345-346.
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33

Bočarov-Stančić AS, Lević J, Stanković SŽ, Tančić SL, Krnjaja V, Salma N. Toxigenic potential of fusarium langsethiae isolates from Serbian wheat kernels. in Cereal Research Communications. 2008;36(SUPPL. 6):345-346.
doi:10.1556/CRC.36.2008.Suppl.B.33 .

Bočarov-Stančić, Aleksandra S., Lević, Jelena, Stanković, Slavica Ž., Tančić, Sonja L., Krnjaja, Vesna, Salma, N., "Toxigenic potential of fusarium langsethiae isolates from Serbian wheat kernels" in Cereal Research Communications, 36, no. SUPPL. 6 (2008):345-346,
https://doi.org/10.1556/CRC.36.2008.Suppl.B.33 . .

TY  - JOUR
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara M.
AU  - Tančić, Sonja L.
AU  - Bočarov-Stančić, Aleksandra S.
PY  - 2008
UR  - http://r.istocar.bg.ac.rs/handle/123456789/178
AB  - Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.
PB  - Akademiai Kiado Rt, Budapest
T2  - Cereal Research Communications
T1  - Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings
VL  - 36
SP  - 515
EP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_178
ER  - 

@article{
author = "Lević, Jelena and Stanković, Slavica Ž. and Krnjaja, Vesna and Kovačević, Tamara M. and Tančić, Sonja L. and Bočarov-Stančić, Aleksandra S.",
year = "2008",
abstract = "Effects of nine isolates of F. langsethiae, originating from Serbia (2005-2006), on vigour of wheat seedlings were the objective of this study. Symptoms of seedlings root rot, reduced of seed germination and retardation of coleoptiles and roots growth were observed. Growth of seedlings roots treated with the filtrate of isolates was reduced in comparison to control. Isolates of F. langsethiae were separated into three VCGs.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Cereal Research Communications",
title = "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings",
volume = "36",
pages = "515-516",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_178"
}

Lević, J., Stanković, S. Ž., Krnjaja, V., Kovačević, T. M., Tančić, S. L.,& Bočarov-Stančić, A. S.. (2008). Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications
Akademiai Kiado Rt, Budapest., 36, 515-516.
https://hdl.handle.net/21.15107/rcub_ristocar_178

Lević J, Stanković SŽ, Krnjaja V, Kovačević TM, Tančić SL, Bočarov-Stančić AS. Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings. in Cereal Research Communications. 2008;36:515-516.
https://hdl.handle.net/21.15107/rcub_ristocar_178 .

Lević, Jelena, Stanković, Slavica Ž., Krnjaja, Vesna, Kovačević, Tamara M., Tančić, Sonja L., Bočarov-Stančić, Aleksandra S., "Pathogenicity and phytotoxicity of Fusarium langsethiae on wheat seedlings" in Cereal Research Communications, 36 (2008):515-516,
https://hdl.handle.net/21.15107/rcub_ristocar_178 .

TY  - CONF
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Ivanović, Dragica
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara M.
AU  - Tančić, Sonja L.
AU  - Bočarov-Stančić, Aleksandra S.
PY  - 2008
UR  - http://r.istocar.bg.ac.rs/handle/123456789/177
AB  - The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).
PB  - Akademiai Kiado Rt, Budapest
C3  - Cereal Research Communications
T1  - Fusarium head blight and grain yield losses of wheat in Serbia
VL  - 36
SP  - 513
EP  - 514
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_177
ER  - 

@conference{
author = "Lević, Jelena and Stanković, Slavica Ž. and Ivanović, Dragica and Krnjaja, Vesna and Kovačević, Tamara M. and Tančić, Sonja L. and Bočarov-Stančić, Aleksandra S.",
year = "2008",
abstract = "The objective of this study was to identify crop production factor, associated with FHB development in Serbian wheat. FHB index and weight losses, varying from 16% to 74% and from 1.8% to 38.3%, respectively, indicated that enviromental conditions were the most important factor affecting disease development and yield losses. F. graminearum dominated on kernels (tip to 55.5%) and whole rachis (34.7%), while F poae was predominant on apical part od rachis (35.2%).",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Cereal Research Communications",
title = "Fusarium head blight and grain yield losses of wheat in Serbia",
volume = "36",
pages = "513-514",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_177"
}

Lević, J., Stanković, S. Ž., Ivanović, D., Krnjaja, V., Kovačević, T. M., Tančić, S. L.,& Bočarov-Stančić, A. S.. (2008). Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications
Akademiai Kiado Rt, Budapest., 36, 513-514.
https://hdl.handle.net/21.15107/rcub_ristocar_177

Lević J, Stanković SŽ, Ivanović D, Krnjaja V, Kovačević TM, Tančić SL, Bočarov-Stančić AS. Fusarium head blight and grain yield losses of wheat in Serbia. in Cereal Research Communications. 2008;36:513-514.
https://hdl.handle.net/21.15107/rcub_ristocar_177 .

Lević, Jelena, Stanković, Slavica Ž., Ivanović, Dragica, Krnjaja, Vesna, Kovačević, Tamara M., Tančić, Sonja L., Bočarov-Stančić, Aleksandra S., "Fusarium head blight and grain yield losses of wheat in Serbia" in Cereal Research Communications, 36 (2008):513-514,
https://hdl.handle.net/21.15107/rcub_ristocar_177 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Tančić, Sonja L.
PY  - 2008
UR  - http://r.istocar.bg.ac.rs/handle/123456789/176
AB  - Pathogenicity of eight F. sporotrichioides isolates, originated from wheat were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate it was determined that four, two and two F. sporotrichioides isolates expressed none, very low and low degree of pathogenicity, respectively. Three types of nit mutants, nitl (25.6%), NitM (6.9%) and nit3 (0.7%), were obtained by the use of the VCG method. A total of eight VCGs of F. sporotrichioides were established in the complementation tests.
PB  - Akademiai Kiado Rt, Budapest
C3  - Cereal Research Communications
T1  - Pathogenic and genetic characterisation of Fusarium sporotrichioides
VL  - 36
SP  - 511
EP  - 512
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_176
ER  - 

@conference{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica Ž. and Tančić, Sonja L.",
year = "2008",
abstract = "Pathogenicity of eight F. sporotrichioides isolates, originated from wheat were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate it was determined that four, two and two F. sporotrichioides isolates expressed none, very low and low degree of pathogenicity, respectively. Three types of nit mutants, nitl (25.6%), NitM (6.9%) and nit3 (0.7%), were obtained by the use of the VCG method. A total of eight VCGs of F. sporotrichioides were established in the complementation tests.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Cereal Research Communications",
title = "Pathogenic and genetic characterisation of Fusarium sporotrichioides",
volume = "36",
pages = "511-512",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_176"
}

Krnjaja, V., Lević, J., Stanković, S. Ž.,& Tančić, S. L.. (2008). Pathogenic and genetic characterisation of Fusarium sporotrichioides. in Cereal Research Communications
Akademiai Kiado Rt, Budapest., 36, 511-512.
https://hdl.handle.net/21.15107/rcub_ristocar_176

Krnjaja V, Lević J, Stanković SŽ, Tančić SL. Pathogenic and genetic characterisation of Fusarium sporotrichioides. in Cereal Research Communications. 2008;36:511-512.
https://hdl.handle.net/21.15107/rcub_ristocar_176 .

Krnjaja, Vesna, Lević, Jelena, Stanković, Slavica Ž., Tančić, Sonja L., "Pathogenic and genetic characterisation of Fusarium sporotrichioides" in Cereal Research Communications, 36 (2008):511-512,
https://hdl.handle.net/21.15107/rcub_ristocar_176 .

TY  - JOUR
AU  - Stanković, Slavica Ž.
AU  - Tančić, Sonja L.
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2008
UR  - http://r.istocar.bg.ac.rs/handle/123456789/158
AB  - DON production by F. graminearum and F. culmorum isolated from wheat grains grown under different agroecological conditions in Serbia during two years were estimated. The incidence of F. graminearum differ considerably between localities, with frequency range from 3.13% to 25.80% and 1.61% to 21,90%, in 2005 and 2006, respectively. F. culmorum was isolated from one and tree localities in 2005 and 2006, respectively. Fourteen isolates of F. graminearum produced considerable amounts of DON, Up to 45.260 ppb, but the tree isolates of F. culmorum proved to be weak producers of this mycotoxin.
PB  - Akademiai Kiado Rt, Budapest
T2  - Cereal Research Communications
T1  - Production of deoxinivalenol by Fusarium graminearum and Fusarium culmorum isolated from wheat kernels in Serbia
VL  - 36
SP  - 395
EP  - 396
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_158
ER  - 

@article{
author = "Stanković, Slavica Ž. and Tančić, Sonja L. and Lević, Jelena and Krnjaja, Vesna",
year = "2008",
abstract = "DON production by F. graminearum and F. culmorum isolated from wheat grains grown under different agroecological conditions in Serbia during two years were estimated. The incidence of F. graminearum differ considerably between localities, with frequency range from 3.13% to 25.80% and 1.61% to 21,90%, in 2005 and 2006, respectively. F. culmorum was isolated from one and tree localities in 2005 and 2006, respectively. Fourteen isolates of F. graminearum produced considerable amounts of DON, Up to 45.260 ppb, but the tree isolates of F. culmorum proved to be weak producers of this mycotoxin.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Cereal Research Communications",
title = "Production of deoxinivalenol by Fusarium graminearum and Fusarium culmorum isolated from wheat kernels in Serbia",
volume = "36",
pages = "395-396",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_158"
}

Stanković, S. Ž., Tančić, S. L., Lević, J.,& Krnjaja, V.. (2008). Production of deoxinivalenol by Fusarium graminearum and Fusarium culmorum isolated from wheat kernels in Serbia. in Cereal Research Communications
Akademiai Kiado Rt, Budapest., 36, 395-396.
https://hdl.handle.net/21.15107/rcub_ristocar_158

Stanković SŽ, Tančić SL, Lević J, Krnjaja V. Production of deoxinivalenol by Fusarium graminearum and Fusarium culmorum isolated from wheat kernels in Serbia. in Cereal Research Communications. 2008;36:395-396.
https://hdl.handle.net/21.15107/rcub_ristocar_158 .

Stanković, Slavica Ž., Tančić, Sonja L., Lević, Jelena, Krnjaja, Vesna, "Production of deoxinivalenol by Fusarium graminearum and Fusarium culmorum isolated from wheat kernels in Serbia" in Cereal Research Communications, 36 (2008):395-396,
https://hdl.handle.net/21.15107/rcub_ristocar_158 .

TY  - JOUR
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra S.
AU  - Tančić, Sonja L.
AU  - Kovačević, Tamara M.
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/138
AB  - A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively.
AB  - Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia
T1  - Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji
IS  - 113
SP  - 93
EP  - 102
DO  - 10.2298/ZMSPN0713093S
ER  - 

@article{
author = "Stanković, Slavica Ž. and Lević, Jelena and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra S. and Tančić, Sonja L. and Kovačević, Tamara M.",
year = "2007",
abstract = "A total of 88 and 40 wheat samples collected immediately prior to harvest in 2005 and 2006, respectively, under different agroecological conditions, were studied in respect to the occurrence of Fusarium spp. and the production of fusariotoxins. The greatest number of samples was infected with species of the genera Fusarium (81.8 and 65.0%), and Alternaria (36.3 and 17.5%) with the intensity ranging from 9.4 to 84.0% in 2005 and from 23.4 to 80.6% in 2006. Out of 13 identified species belonging to the genus Fusarium, F. graminearum had the highest frequency (35.2 and 12.5%) and the intensity up to 67.2%, and 21.9%, in 2005 and 2006, respectively, followed by F. poae but only in 2005 (20.4%), and F. proliferatum in 2006 (19.7%). The natural occurrence of mycotoxins in positive samples varied from 37 to 331 ppb for zearalenone and from 31 to 125 ppb for diacetoxyscirpenol (DAS) and T-2 toxin. The concentration of mycotoxins amounted, on average, to 133.4, 61.0 and 45.7 ppb for zearelenone, DAS and T-2 toxin, respectively., Osamdeset osam i 40 uzoraka pšenice, prikupljenih neposredno pred žetvu u 2005. i 2006. godini u različitim agroekološkim uslovima u Srbiji proučavani su radi praćenja pojave Fusarium spp. i stvaranja fuzariotoksina. Najveći broj uzoraka bio je zaražen vrstama roda Fusarium (81,8 i 65,0%), a zatim sa Alternaria spp. (36,3 i 17,5%) sa intenzitetom 9,4-84,0% u 2005. i 23,4-80,6% u 2006. godini. Od ukupno 13 identifikovanih vrsta iz roda Fusarium najučestalija je bila F. graminearum (35,2 i 12,5%) sa intenzitetom do 67,2% u 2005. i do 21,9% u 2006. godini, a zatim F. poae, ali samo u 2005. godini (20,4%), i F. proliferatum u 2006. godini (19.7%). Prirodna pojava mikotoksina u pozitivnim uzorcima je varirala od 37 do 331 ppb za zearalenon i od 31 do 125 ppb za diacetoksiscirpenol (DAS) i T-2 toksin. U proseku, koncentracija mikotoksina je bila 133,4 ppb za zearelonon, 61,0 ppb za DAS i 45,7 ppb za T-2 toksin.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia, Učestalost toksigenih vrsta Fusarium i fuzariotoksina u zrnu pšenice u Srbiji",
number = "113",
pages = "93-102",
doi = "10.2298/ZMSPN0713093S"
}

Stanković, S. Ž., Lević, J., Krnjaja, V., Bočarov-Stančić, A. S., Tančić, S. L.,& Kovačević, T. M.. (2007). Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 93-102.
https://doi.org/10.2298/ZMSPN0713093S

Stanković SŽ, Lević J, Krnjaja V, Bočarov-Stančić AS, Tančić SL, Kovačević TM. Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia. in Zbornik Matice srpske za prirodne nauke. 2007;(113):93-102.
doi:10.2298/ZMSPN0713093S .

Stanković, Slavica Ž., Lević, Jelena, Krnjaja, Vesna, Bočarov-Stančić, Aleksandra S., Tančić, Sonja L., Kovačević, Tamara M., "Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):93-102,
https://doi.org/10.2298/ZMSPN0713093S . .

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra S.
AU  - Lević, Jelena
AU  - Stanković, Slavica Ž.
AU  - Krnjaja, Vesna
AU  - Kovačević, Tamara M.
AU  - Tančić, Sonja L.
PY  - 2007
UR  - http://r.istocar.bg.ac.rs/handle/123456789/141
AB  - Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively.
AB  - U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The toxigenic potential of Fusarium poae originated from wheat
T1  - Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice
IS  - 113
SP  - 113
EP  - 123
DO  - 10.2298/ZMSPN0713113B
ER  - 

@article{
author = "Bočarov-Stančić, Aleksandra S. and Lević, Jelena and Stanković, Slavica Ž. and Krnjaja, Vesna and Kovačević, Tamara M. and Tančić, Sonja L.",
year = "2007",
abstract = "Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively., U ovom radu je u in vitro uslovima proučena sposobnost izolata F. poae za biosintezu jedne grupe fuzariotoksina - trihotecena tipa A (T-2 toksin i diacetoksiscirpenol - DAS), kao i zearalenona (ZEA). Toksikološki profil ove vrste je kod nas nedovoljno ispitan s obzirom na njenu zastupljenost i toksigena svojstva prema literaturnim podacima. Proučavanjima je bilo obuhvaćeno 11 izolata F. poae, poreklom sa pšenice iz 9 lokaliteta, uglavnom sa područja Vojvodine. Najveći broj uzoraka prikupljen je 2005. godine, kada su klimatski uslovi pogodovali intenzivnijoj pojavi fuzarioza klasa ove poljoprivredne kulture. Za određivanje toksikološkog potencijala F. poae kulture odabranih izolata su gajene u tečnim podlogama (GPK i SPK) tokom 3 dana na sobnoj temperaturi (21-26°C) i na rotacionoj tresilici (180 obrtaja min-1). Sirovi toksini su izolovani iz filtrata tečnih kultura ispitanih izolata pomoću etil acetata, dok je kvantifikacija mikotoksina izvršena metodom tankoslojne hromatografije. Tečna kultura izolata GZ-LES (F. graminearum) je korišćena kao kontrolna kultura za utvrđivanje potencijala za biosintezu zearalenona, a KF-38/1/R (F. sporotrichioides) za oba trihotecena tipa A (T-2 toksin i DAS). Dobijeni rezultati pokazuju da F. poae, za razliku od F. graminearum, ne poseduje potencijal za biosintezu zearalenona. Prisustvo DAS-a je utvrđeno samo kod jedne kulture F. poae (MRIZP-666) i kontrolnog izolata F. sporotrichioides (KF-38/1/R) koji su gajeni u tečnom GPK medijumu. T-2 toksin je detektovan kod izolata MRIZP-666 pri gajenju u obema podlogama, kao i izolata MRIZP-37 u GPK, odnosno MRIZP-860 u SPK medijumu. Kontrolna kultura KF-38/1/R (F. sporotrichioides) proizvodila je T-2 toksin u koncentraciji od 4000 μg L-1. Na osnovu iznetih podataka može se zaključiti da je potencijal F. poae za biosintezu trihotecena tipa A bio nizak u datim uslovima s obzirom da koncentracija DAS-a nije prelazila 80 μg L-1, odnosno T-2 toksina 240 μg L-1. Imajući u vidu prikazane rezultate, smatramo da je za dobijanje konačnog odgovora na pitanje o toksikološkom profilu izolata F. poae u Srbiji neophodno preduzeti dodatna ispitivanja, ne samo sa novim izolatima iz godina koje slede, nego i u drugim uslovima kultivisanja, u prvom redu na sterilnom prirodnom supstratu kao što su pšenica i kukuruz.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The toxigenic potential of Fusarium poae originated from wheat, Toksigeni potencijal izolata Fusarium poae poreklom sa pšenice",
number = "113",
pages = "113-123",
doi = "10.2298/ZMSPN0713113B"
}

Bočarov-Stančić, A. S., Lević, J., Stanković, S. Ž., Krnjaja, V., Kovačević, T. M.,& Tančić, S. L.. (2007). The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 113-123.
https://doi.org/10.2298/ZMSPN0713113B

Bočarov-Stančić AS, Lević J, Stanković SŽ, Krnjaja V, Kovačević TM, Tančić SL. The toxigenic potential of Fusarium poae originated from wheat. in Zbornik Matice srpske za prirodne nauke. 2007;(113):113-123.
doi:10.2298/ZMSPN0713113B .

Bočarov-Stančić, Aleksandra S., Lević, Jelena, Stanković, Slavica Ž., Krnjaja, Vesna, Kovačević, Tamara M., Tančić, Sonja L., "The toxigenic potential of Fusarium poae originated from wheat" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):113-123,
https://doi.org/10.2298/ZMSPN0713113B . .