Ivanović, Mirko

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Fusariotoxins in wheat grain in Serbia

Stepanić, Ana; Stanković, Slavica Ž.; Lević, Jelena; Ivanović, Mirko; Krnjaja, Vesna

(Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade, 2011)

TY  - JOUR
AU  - Stepanić, Ana
AU  - Stanković, Slavica Ž.
AU  - Lević, Jelena
AU  - Ivanović, Mirko
AU  - Krnjaja, Vesna
PY  - 2011
UR  - http://r.istocar.bg.ac.rs/handle/123456789/277
AB  - Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption.
AB  - Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.
PB  - Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Fusariotoxins in wheat grain in Serbia
T1  - Fuzariotoksini u zrnu pšenice u Srbiji
VL  - 26
IS  - 4
SP  - 317
EP  - 323
DO  - 10.2298/PIF1104317S
ER  - 
@article{
author = "Stepanić, Ana and Stanković, Slavica Ž. and Lević, Jelena and Ivanović, Mirko and Krnjaja, Vesna",
year = "2011",
abstract = "Samples of wheat grain (41), collected during the 2010 harvest from seven localities in Serbia, were analyzed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON) and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showed that all analysed samples were positive for the presence of at least one of four observed fusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentration of 442.6μg kg-1) and T-2 (90.2%, with the average concentration of 24.2 μg kg-1). DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, but their average concentrations were higher (1988.1 μg DON kg-1 and 882.7 μg FB1 kg-1). The established correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA (r = 0.22) were not statistically significant. A negative correlation was established between concentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detected concentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation (WHO), while concentrations of FB1 and DON detected in five that is, 17 samples, respectively, were above the permissible limit for human consumption., Uzorci zrna pšenice (41), sakupljeni tokom žetve 2010. godine iz 7 različitih lokaliteta na teritoriji Srbije, analizirani su na prisustvo ZEA, T-2 toksina, deoksinivalenola (DON) i FB1. Primenom ELISA metode utvrđeno je da su svi analizirani uzorci bili pozitivni na prisustvo bar jednog od četiri ispitivana fuzariotoksina. Najzastupljeniji mikotoksini su bili ZEA (90,2%, prosečne koncentracije 442,6 μg kg-1) i T-2 (90,2%, prosečna koncentracija 24,2 kg-1). U nešto manjem broju uzoraka utvrđeni su DON (73,2%) i FB1 (84,4%), ali u većoj prosečnoj koncentraciji (1988,1 μg DON kg-1 i 882,7 μg FB1 kg-1). Nije utvrđena statistički značajna korelacija između koncentracije DON i FB1 (r = 0,32) ili DON i ZEA (r = 0,22). Negativna korelacija je utvrđena između koncentracije T-2 i FB1 (r = -0,24), kao i između T-2 i DON (r = -0,36). Detektovane koncentracije ZEA i T-2 bile su niže od nivoa koji propisuje Svetska zdravstvena organizacija, dok su FB1 i DON detektovani kod 5, odnosno 17 uzoraka u koncentraciji većoj od dozvoljenih u ljudskoj hrani.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Fusariotoxins in wheat grain in Serbia, Fuzariotoksini u zrnu pšenice u Srbiji",
volume = "26",
number = "4",
pages = "317-323",
doi = "10.2298/PIF1104317S"
}
Stepanić, A., Stanković, S. Ž., Lević, J., Ivanović, M.,& Krnjaja, V.. (2011). Fusariotoxins in wheat grain in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade / Plant Protection Society of Serbia, Belgrade., 26(4), 317-323.
https://doi.org/10.2298/PIF1104317S
Stepanić A, Stanković SŽ, Lević J, Ivanović M, Krnjaja V. Fusariotoxins in wheat grain in Serbia. in Pesticidi i fitomedicina. 2011;26(4):317-323.
doi:10.2298/PIF1104317S .
Stepanić, Ana, Stanković, Slavica Ž., Lević, Jelena, Ivanović, Mirko, Krnjaja, Vesna, "Fusariotoxins in wheat grain in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):317-323,
https://doi.org/10.2298/PIF1104317S . .
6

Alfalfa root diseases and control of pathogens

Krnjaja, Vesna; Ivanović, Mirko; Lević, Jelena; Tomić, Zorica

(Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2005)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Ivanović, Mirko
AU  - Lević, Jelena
AU  - Tomić, Zorica
PY  - 2005
UR  - http://r.istocar.bg.ac.rs/handle/123456789/69
AB  - Several pathogenic fungi (Phytophthora spp., Pythium spp., Fusarium spp., Rhizoctonia spp., Colletotrichum spp., Verticillium spp., Scelorotinia spp.), bacteria (Corynebacterium spp.) and nematodes Pratylenchus spp., Meloidogyne spp., etc.) could each be the cause both of some specific types of the rot of alfalfa root, crown and ground parts of stem as well as of non-specific symptoms (low growth, chlorosis and wilt) on aboveground parts of plants. The consequences of the occurrence of these diseases could have a great economic effect and are demonstrated as a scattered crop, reduced yield, quality and longevity of alfalfa. Abiotic factors (low temperature, lack or excess of moisture, soil type, application inadequacy of the production technology of alfalfa etc.), either individually or in a complex comprising several of them, could also be the cause of alfalfa diseases and if in interaction with biotic factors the damages arising there of could even be more substantial. The economically most important diseases of alfalfa rot caused by pathogenic fungi, bacteria and some abiotic factors, as well as a review of integrated control of alfalfa pathogens have been described.
AB  - Različite patogene gljive ( Phytophthora spp., Pythium spp., Fusarium spp., Rhizoctonia spp., Colletotrichum spp., Verticillium spp., Scelorotinia spp. i dr.), bakterije (Corynebacterium spp.) i nematode ( Pratylenchus spp., Meloidogyne spp. i dr.), mogu prouzrokovati specifične tipove truleži korena, korenovog vrata i prizemnog dela stabla lucerke i nespecifične simptome (niži porast, hloroza i uvelost) na nadzemnim delovima biljaka. Posledice ovih bolesti od velikog su ekonomskog značaja, a ispoljavaju se u vidu proređenog useva, smanjenja prinosa, kvaliteta i dugovečnosti lucerke. Abiotski činioci (niske temperature, nedostatak i suvišak vode, tip zemljišta, primena neodgovarajuće tehnologije proizvodnje lucerke i dr.), pojedinačno ili u kompleksu više njih, mogu, takođe, prouzrokovati bolesti lucerke, a ukoliko su u sadejstvu s biotskim činiocima nastale štete mogu biti znatno veće. U radu su opisane ekonomski najznačajnije bolesti korena lucerke, čiji su prouzrokovači patogene gljive, bakterije i neki abiotski činioci, kao i prikaz pojedinih i integralnih mera, koje se preporučuju za suzbijanje patogena lucerke. Zbog oskudnih podataka o bolestima korena lucerke u domaćoj literaturi, uglavnom su korišćeni podaci iz strane literature.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Alfalfa root diseases and control of pathogens
T1  - Bolesti korena lucerke i mere suzbijanja patogena
VL  - 33
IS  - 5
SP  - 565
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_69
ER  - 
@article{
author = "Krnjaja, Vesna and Ivanović, Mirko and Lević, Jelena and Tomić, Zorica",
year = "2005",
abstract = "Several pathogenic fungi (Phytophthora spp., Pythium spp., Fusarium spp., Rhizoctonia spp., Colletotrichum spp., Verticillium spp., Scelorotinia spp.), bacteria (Corynebacterium spp.) and nematodes Pratylenchus spp., Meloidogyne spp., etc.) could each be the cause both of some specific types of the rot of alfalfa root, crown and ground parts of stem as well as of non-specific symptoms (low growth, chlorosis and wilt) on aboveground parts of plants. The consequences of the occurrence of these diseases could have a great economic effect and are demonstrated as a scattered crop, reduced yield, quality and longevity of alfalfa. Abiotic factors (low temperature, lack or excess of moisture, soil type, application inadequacy of the production technology of alfalfa etc.), either individually or in a complex comprising several of them, could also be the cause of alfalfa diseases and if in interaction with biotic factors the damages arising there of could even be more substantial. The economically most important diseases of alfalfa rot caused by pathogenic fungi, bacteria and some abiotic factors, as well as a review of integrated control of alfalfa pathogens have been described., Različite patogene gljive ( Phytophthora spp., Pythium spp., Fusarium spp., Rhizoctonia spp., Colletotrichum spp., Verticillium spp., Scelorotinia spp. i dr.), bakterije (Corynebacterium spp.) i nematode ( Pratylenchus spp., Meloidogyne spp. i dr.), mogu prouzrokovati specifične tipove truleži korena, korenovog vrata i prizemnog dela stabla lucerke i nespecifične simptome (niži porast, hloroza i uvelost) na nadzemnim delovima biljaka. Posledice ovih bolesti od velikog su ekonomskog značaja, a ispoljavaju se u vidu proređenog useva, smanjenja prinosa, kvaliteta i dugovečnosti lucerke. Abiotski činioci (niske temperature, nedostatak i suvišak vode, tip zemljišta, primena neodgovarajuće tehnologije proizvodnje lucerke i dr.), pojedinačno ili u kompleksu više njih, mogu, takođe, prouzrokovati bolesti lucerke, a ukoliko su u sadejstvu s biotskim činiocima nastale štete mogu biti znatno veće. U radu su opisane ekonomski najznačajnije bolesti korena lucerke, čiji su prouzrokovači patogene gljive, bakterije i neki abiotski činioci, kao i prikaz pojedinih i integralnih mera, koje se preporučuju za suzbijanje patogena lucerke. Zbog oskudnih podataka o bolestima korena lucerke u domaćoj literaturi, uglavnom su korišćeni podaci iz strane literature.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Alfalfa root diseases and control of pathogens, Bolesti korena lucerke i mere suzbijanja patogena",
volume = "33",
number = "5",
pages = "565-576",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_69"
}
Krnjaja, V., Ivanović, M., Lević, J.,& Tomić, Z.. (2005). Alfalfa root diseases and control of pathogens. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 33(5), 565-576.
https://hdl.handle.net/21.15107/rcub_ristocar_69
Krnjaja V, Ivanović M, Lević J, Tomić Z. Alfalfa root diseases and control of pathogens. in Biljni lekar. 2005;33(5):565-576.
https://hdl.handle.net/21.15107/rcub_ristocar_69 .
Krnjaja, Vesna, Ivanović, Mirko, Lević, Jelena, Tomić, Zorica, "Alfalfa root diseases and control of pathogens" in Biljni lekar, 33, no. 5 (2005):565-576,
https://hdl.handle.net/21.15107/rcub_ristocar_69 .

Virulence of Fusarium species to alfalfa seedlings

Krnjaja, Vesna; Lević, Jelena; Ivanović, Mirko; Tomić, Zorica

(Matica srpska, Novi Sad, 2005)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Ivanović, Mirko
AU  - Tomić, Zorica
PY  - 2005
UR  - http://r.istocar.bg.ac.rs/handle/123456789/73
AB  - In in vitro conditions, virulence of 91 isolates of species Fusarium genus (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. proliferatum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides and F. graminearum) towards alfalfa seedlings was investigated. Isolates of investigated species originated from diseased alfalfa plants collected on four locations in Serbia based on symptoms of wilting caused by fusarium and root rotting. Pathogenicity and virulence of investigated isolates of Fusarium spp. were determined by visual evaluation of inoculated seedlings of cultivars K28 in laboratory conditions. All isolated of investigated species had pathogenic effect on alfalfa seedlings, which expressed symptoms such as necrosis of root, moist rotting and "melting of seedlings". Colour of necrotic root tissue varied from light brown, brown, lipstick red to explicit black, depending on the Fusarium species. Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates.
AB  - U in vitro uslovima proučena je virulentnost 91-og izolata vrsta roda Fusarium (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. proliferatum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides i F. graminearum) prema klijancima lucerke. Izolati ispitivanih vrsta poreklom su iz obolelih biljaka lucerke koje su prikupljene iz četiri lokaliteta u Srbiji na osnovu simptoma fuzarioznog uvenuća i truleži korena. Patogenost i virulentnost ispitivanih izolata Fusarium spp. utvrđeni su vizuelnim ocenjivanjem inokulisanih klijanaca sorte K28 u laboratorijskim uslovima. Svi izolati ispitivanih vrsta patogeni su prema klijancima lucerke, koji su ispoljili simptome u vidu nekroze korena vlažne truleži i "topljenja klijanaca". Boja nekrotiranog tkiva korena varira od svetlo smeđe, smeđe, crveno-smeđe, karmin crvene do izrazito crne, zavisno od vrste roda Fusarium. Jaku virulentnost ispoljilo je 48 izolata, srednju 31 izolat, a slabu 12 izolata.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Virulence of Fusarium species to alfalfa seedlings
T1  - Virulentnost vrsta roda Fusarium prema klijancima lucerke
IS  - 109
SP  - 65
EP  - 72
DO  - 10.2298/ZMSPN0519065K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Ivanović, Mirko and Tomić, Zorica",
year = "2005",
abstract = "In in vitro conditions, virulence of 91 isolates of species Fusarium genus (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. proliferatum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides and F. graminearum) towards alfalfa seedlings was investigated. Isolates of investigated species originated from diseased alfalfa plants collected on four locations in Serbia based on symptoms of wilting caused by fusarium and root rotting. Pathogenicity and virulence of investigated isolates of Fusarium spp. were determined by visual evaluation of inoculated seedlings of cultivars K28 in laboratory conditions. All isolated of investigated species had pathogenic effect on alfalfa seedlings, which expressed symptoms such as necrosis of root, moist rotting and "melting of seedlings". Colour of necrotic root tissue varied from light brown, brown, lipstick red to explicit black, depending on the Fusarium species. Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates., U in vitro uslovima proučena je virulentnost 91-og izolata vrsta roda Fusarium (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. proliferatum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides i F. graminearum) prema klijancima lucerke. Izolati ispitivanih vrsta poreklom su iz obolelih biljaka lucerke koje su prikupljene iz četiri lokaliteta u Srbiji na osnovu simptoma fuzarioznog uvenuća i truleži korena. Patogenost i virulentnost ispitivanih izolata Fusarium spp. utvrđeni su vizuelnim ocenjivanjem inokulisanih klijanaca sorte K28 u laboratorijskim uslovima. Svi izolati ispitivanih vrsta patogeni su prema klijancima lucerke, koji su ispoljili simptome u vidu nekroze korena vlažne truleži i "topljenja klijanaca". Boja nekrotiranog tkiva korena varira od svetlo smeđe, smeđe, crveno-smeđe, karmin crvene do izrazito crne, zavisno od vrste roda Fusarium. Jaku virulentnost ispoljilo je 48 izolata, srednju 31 izolat, a slabu 12 izolata.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Virulence of Fusarium species to alfalfa seedlings, Virulentnost vrsta roda Fusarium prema klijancima lucerke",
number = "109",
pages = "65-72",
doi = "10.2298/ZMSPN0519065K"
}
Krnjaja, V., Lević, J., Ivanović, M.,& Tomić, Z.. (2005). Virulence of Fusarium species to alfalfa seedlings. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(109), 65-72.
https://doi.org/10.2298/ZMSPN0519065K
Krnjaja V, Lević J, Ivanović M, Tomić Z. Virulence of Fusarium species to alfalfa seedlings. in Zbornik Matice srpske za prirodne nauke. 2005;(109):65-72.
doi:10.2298/ZMSPN0519065K .
Krnjaja, Vesna, Lević, Jelena, Ivanović, Mirko, Tomić, Zorica, "Virulence of Fusarium species to alfalfa seedlings" in Zbornik Matice srpske za prirodne nauke, no. 109 (2005):65-72,
https://doi.org/10.2298/ZMSPN0519065K . .

Incidence of fusarium species on red clover seed

Krnjaja, Vesna; Lević, Jelena; Ivanović, Mirko; Tomić, Zorica; Mrfat-Vukelić, Slavica

(Institute for Animal Husbandry, Belgrade, 2004)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Ivanović, Mirko
AU  - Tomić, Zorica
AU  - Mrfat-Vukelić, Slavica
PY  - 2004
UR  - http://r.istocar.bg.ac.rs/handle/123456789/40
AB  - Phytopathological investigation of seed of three commercial red clover cultivars - K9, K17 and NS Kolubara, and two pre-treatments of seeds rinsing and surface disinfection of seeds (T1) and surface disinfection of seeds (T2) have determined that frequency of species within the genus Fusarium varied from 0 to 13%. By use of T1 pre-treatment the presence of Fusarium species on the seed was observed in neither of examined cultivars. In this pre-treatment the presence of 1% Penicillium spp. was observed on seed of cultivar K17 only, while the seeds K9 and NS Kolubara were non-infected. By use of T2 pre-treatment, the frequency of Fusarium species varied from 1 to 13%, depending on cultivar. From the seeds of all three examined cultivars, Fusarium verticillioides (2-13%) and F. proliferatum (2-6%) were isolated and from the seed of cultivar K9, F. oxysporum (4%) F. solani (2%) and undetermined Fusarium species (3%) were isolated, while F. subglutinans (1%) was isolated only from the seed of NS Kolubara. Besides Fusarium species, by application of pre-treatment T2, in all examined cultivars the species from genera Cladosporium (6-14%), Alternaria (6-7%) and Acremonium (1-5%) were isolated, and Penicillium spp. (1%) from the seed of cultivar K17. The results obtained indicate that frequency of mycobiota on the seed of red clover depended more on the seed pre-treatment than on the examined cultivars. Considering the significant differences gained in health state of seed by application of T1 and T2 pre-treatments, further investigations should be directed towards the development of method which would show real health state of seed, that is, natural contamination of seed. In this case it is the use of pre-treatment T1.
AB  - Fitopatološkim ispitivanjima semena tri komercijalna kultivara crvene deteline - K9, K17 i NS Kolubara, i dva pretretmana semena - ispiranje i površinska dezinfekcija semena (T1) i površinska dezinfekcija semena (T2) utvrđeno je da je učestalost vrsta iz roda Fusarium varirala od 0 do 13%. Primenom T1 pretretmana nije utvrđeno prisustvo Fusarium vrsta na semenu ni u jednog ispitivanog kultivara. U ovom pretretmanu je samo na semenu kultivara K17 ustanovljeno prisustvo 1% Penicillium spp., dok je seme K9 i NS Kolubara bilo bez zaraze. Primenom T2 pretretmana, učestalost Fusarium vrsta je varirala od 1 do 13%, zavisno od kultivara. Iz semena sva tri ispitivana kultivara izolovane su Fusarium verticillioides (2-13%) i F. proliferatum (2-6%) dok su iz semena kultivara K9 izolovane F. oxysporum (4%), F. solani (2%) i nedeterminisane Fusarium vrste (3%), a F. subglutinans (1%) jedino iz semena NS Kolubara. Pored Fusarium vrsta primenom pretretmana kod svih ispitivanih kultivara, izolovane su vrste iz rodova Cladosporium (6-14%), Alternaria (6-7%) i Acremonium (1-5%), a Penicillium spp. (1%) iz semena kultivara K17. Dobijeni rezultati ukazuju da je učestalost mikobiota na semenu crvene deteline više zavisila od pretretmana semena nego od ispitivane sorte. S obzirom da su konstatovane značajne razlike u zdravstvenom stanju semena primenom T1 i T2 pretretmana buduća istraživanja trebalo bi usmeriti u pravcu razvoja metode koja pokazuje realno zdravstveno stanje semena, odnosno prirodnu zarazu semena. U ovom slučaju to je primena pretretmana T1.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Incidence of fusarium species on red clover seed
T1  - Učestalost fusarium vrsta na semenu crvene deteline
VL  - 20
IS  - 1-2
SP  - 101
EP  - 108
DO  - 10.2298/BAH0402101K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Ivanović, Mirko and Tomić, Zorica and Mrfat-Vukelić, Slavica",
year = "2004",
abstract = "Phytopathological investigation of seed of three commercial red clover cultivars - K9, K17 and NS Kolubara, and two pre-treatments of seeds rinsing and surface disinfection of seeds (T1) and surface disinfection of seeds (T2) have determined that frequency of species within the genus Fusarium varied from 0 to 13%. By use of T1 pre-treatment the presence of Fusarium species on the seed was observed in neither of examined cultivars. In this pre-treatment the presence of 1% Penicillium spp. was observed on seed of cultivar K17 only, while the seeds K9 and NS Kolubara were non-infected. By use of T2 pre-treatment, the frequency of Fusarium species varied from 1 to 13%, depending on cultivar. From the seeds of all three examined cultivars, Fusarium verticillioides (2-13%) and F. proliferatum (2-6%) were isolated and from the seed of cultivar K9, F. oxysporum (4%) F. solani (2%) and undetermined Fusarium species (3%) were isolated, while F. subglutinans (1%) was isolated only from the seed of NS Kolubara. Besides Fusarium species, by application of pre-treatment T2, in all examined cultivars the species from genera Cladosporium (6-14%), Alternaria (6-7%) and Acremonium (1-5%) were isolated, and Penicillium spp. (1%) from the seed of cultivar K17. The results obtained indicate that frequency of mycobiota on the seed of red clover depended more on the seed pre-treatment than on the examined cultivars. Considering the significant differences gained in health state of seed by application of T1 and T2 pre-treatments, further investigations should be directed towards the development of method which would show real health state of seed, that is, natural contamination of seed. In this case it is the use of pre-treatment T1., Fitopatološkim ispitivanjima semena tri komercijalna kultivara crvene deteline - K9, K17 i NS Kolubara, i dva pretretmana semena - ispiranje i površinska dezinfekcija semena (T1) i površinska dezinfekcija semena (T2) utvrđeno je da je učestalost vrsta iz roda Fusarium varirala od 0 do 13%. Primenom T1 pretretmana nije utvrđeno prisustvo Fusarium vrsta na semenu ni u jednog ispitivanog kultivara. U ovom pretretmanu je samo na semenu kultivara K17 ustanovljeno prisustvo 1% Penicillium spp., dok je seme K9 i NS Kolubara bilo bez zaraze. Primenom T2 pretretmana, učestalost Fusarium vrsta je varirala od 1 do 13%, zavisno od kultivara. Iz semena sva tri ispitivana kultivara izolovane su Fusarium verticillioides (2-13%) i F. proliferatum (2-6%) dok su iz semena kultivara K9 izolovane F. oxysporum (4%), F. solani (2%) i nedeterminisane Fusarium vrste (3%), a F. subglutinans (1%) jedino iz semena NS Kolubara. Pored Fusarium vrsta primenom pretretmana kod svih ispitivanih kultivara, izolovane su vrste iz rodova Cladosporium (6-14%), Alternaria (6-7%) i Acremonium (1-5%), a Penicillium spp. (1%) iz semena kultivara K17. Dobijeni rezultati ukazuju da je učestalost mikobiota na semenu crvene deteline više zavisila od pretretmana semena nego od ispitivane sorte. S obzirom da su konstatovane značajne razlike u zdravstvenom stanju semena primenom T1 i T2 pretretmana buduća istraživanja trebalo bi usmeriti u pravcu razvoja metode koja pokazuje realno zdravstveno stanje semena, odnosno prirodnu zarazu semena. U ovom slučaju to je primena pretretmana T1.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Incidence of fusarium species on red clover seed, Učestalost fusarium vrsta na semenu crvene deteline",
volume = "20",
number = "1-2",
pages = "101-108",
doi = "10.2298/BAH0402101K"
}
Krnjaja, V., Lević, J., Ivanović, M., Tomić, Z.,& Mrfat-Vukelić, S.. (2004). Incidence of fusarium species on red clover seed. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 20(1-2), 101-108.
https://doi.org/10.2298/BAH0402101K
Krnjaja V, Lević J, Ivanović M, Tomić Z, Mrfat-Vukelić S. Incidence of fusarium species on red clover seed. in Biotechnology in Animal Husbandry. 2004;20(1-2):101-108.
doi:10.2298/BAH0402101K .
Krnjaja, Vesna, Lević, Jelena, Ivanović, Mirko, Tomić, Zorica, Mrfat-Vukelić, Slavica, "Incidence of fusarium species on red clover seed" in Biotechnology in Animal Husbandry, 20, no. 1-2 (2004):101-108,
https://doi.org/10.2298/BAH0402101K . .
2

The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr

Krnjaja, Vesna; Tomić, Zorica; Lević, Jelena; Ivanović, Mirko

(Agronomski fakultet, Čačak, 2004)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Zorica
AU  - Lević, Jelena
AU  - Ivanović, Mirko
PY  - 2004
UR  - http://r.istocar.bg.ac.rs/handle/123456789/42
AB  - The resistance of seven cultivars of Medicago sativa L. against fungi of genus Fusarium has been investigated. In order to evaluate the resistance level we have used the method of plant inoculation on seven day old plants grown in the greenhouse. The intensity of disease was estimated on the basis of visual examination of plants. In the reaction of cultivars considerable variability was not observed. Among examined cultivars Zaječarska 83 cultivar was moderately sensitive, while the cultivars K22, K23, K28 Triade, NS Mediana ZMS V and NS Banat ZMS II expressed high sensitivity.
AB  - Ispitivana je otpornost sedam sorti Medicago sativa L. prema gljivama roda Fusarium. Za ocenu nivoa otpornosti korišćena je metoda inokulacije biljaka starih sedam nedelja koje su bile odgajene u uslovima staklenika. Intenzitet oboljenja ocenjen je na osnovu vizuelnog pregleda biljaka. U reakciji sorti nije se ispoljila znatna varijabilnost. Među ispitivanim sortama srednje osetljiva sorta je Zaječarska 83. Visoku osetljivost ispoljile su sorte K22 K23, K28, Triade, NS Mediana ZMS V i NS Banat ZMS II.
PB  - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr
T1  - Otpornost nekih sorti lucerke (medicago sativa l) prema gljivama roda fusarium link
VL  - 9
IS  - Special Issue
SP  - 443
EP  - 449
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_42
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Zorica and Lević, Jelena and Ivanović, Mirko",
year = "2004",
abstract = "The resistance of seven cultivars of Medicago sativa L. against fungi of genus Fusarium has been investigated. In order to evaluate the resistance level we have used the method of plant inoculation on seven day old plants grown in the greenhouse. The intensity of disease was estimated on the basis of visual examination of plants. In the reaction of cultivars considerable variability was not observed. Among examined cultivars Zaječarska 83 cultivar was moderately sensitive, while the cultivars K22, K23, K28 Triade, NS Mediana ZMS V and NS Banat ZMS II expressed high sensitivity., Ispitivana je otpornost sedam sorti Medicago sativa L. prema gljivama roda Fusarium. Za ocenu nivoa otpornosti korišćena je metoda inokulacije biljaka starih sedam nedelja koje su bile odgajene u uslovima staklenika. Intenzitet oboljenja ocenjen je na osnovu vizuelnog pregleda biljaka. U reakciji sorti nije se ispoljila znatna varijabilnost. Među ispitivanim sortama srednje osetljiva sorta je Zaječarska 83. Visoku osetljivost ispoljile su sorte K22 K23, K28, Triade, NS Mediana ZMS V i NS Banat ZMS II.",
publisher = "Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr, Otpornost nekih sorti lucerke (medicago sativa l) prema gljivama roda fusarium link",
volume = "9",
number = "Special Issue",
pages = "443-449",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_42"
}
Krnjaja, V., Tomić, Z., Lević, J.,& Ivanović, M.. (2004). The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr. in Acta agriculturae Serbica
Agronomski fakultet, Čačak., 9(Special Issue), 443-449.
https://hdl.handle.net/21.15107/rcub_ristocar_42
Krnjaja V, Tomić Z, Lević J, Ivanović M. The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr. in Acta agriculturae Serbica. 2004;9(Special Issue):443-449.
https://hdl.handle.net/21.15107/rcub_ristocar_42 .
Krnjaja, Vesna, Tomić, Zorica, Lević, Jelena, Ivanović, Mirko, "The resistance of some cultivars of medicago sativa l. Against fungi genus fusarium link. Ex fr" in Acta agriculturae Serbica, 9, no. Special Issue (2004):443-449,
https://hdl.handle.net/21.15107/rcub_ristocar_42 .