TY  - JOUR
AU  - Kostic, Aleksandar Z.
AU  - Petrović, Tanja S.
AU  - Krnjaja, Vesna
AU  - Nedic, Nebojša M.
AU  - Tešić, Živoslav Lj.
AU  - Milojkovic-Opsenica, Dusanka M.
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladana P.
AU  - Pesic, Mirjana B.
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/519
AB  - Assessment of microbiological quality of bee collected pollen is very important, because of its use as a supplement in the human diet. In this study, 26 samples collected from different location in Serbia were tested for the presence of mold through mycologial analysis. The presence of aflatoxin B1, one of the most dangerous and the most widespread mycotoxin was also determined. It was established that 10 of the investigated samples were contaminated with some genera or species of mold, but all of the investigated samples were contaminated with aflatoxin B1. Considering that there is no unique and official procedure for mycological analysis of bee collected pollen, these findings suggest the need for their establishment. Mycological analysis should be followed by mycotoxicological analysis since the absence of mold does not confirm the absence of aflatoxin B1 in bee pollen.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions
VL  - 56
IS  - 1
SP  - 13
EP  - 20
DO  - 10.1080/00218839.2016.1259897
ER  - 

@article{
author = "Kostic, Aleksandar Z. and Petrović, Tanja S. and Krnjaja, Vesna and Nedic, Nebojša M. and Tešić, Živoslav Lj. and Milojkovic-Opsenica, Dusanka M. and Barac, Miroljub B. and Stanojević, Sladana P. and Pesic, Mirjana B.",
year = "2017",
abstract = "Assessment of microbiological quality of bee collected pollen is very important, because of its use as a supplement in the human diet. In this study, 26 samples collected from different location in Serbia were tested for the presence of mold through mycologial analysis. The presence of aflatoxin B1, one of the most dangerous and the most widespread mycotoxin was also determined. It was established that 10 of the investigated samples were contaminated with some genera or species of mold, but all of the investigated samples were contaminated with aflatoxin B1. Considering that there is no unique and official procedure for mycological analysis of bee collected pollen, these findings suggest the need for their establishment. Mycological analysis should be followed by mycotoxicological analysis since the absence of mold does not confirm the absence of aflatoxin B1 in bee pollen.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions",
volume = "56",
number = "1",
pages = "13-20",
doi = "10.1080/00218839.2016.1259897"
}

Kostic, A. Z., Petrović, T. S., Krnjaja, V., Nedic, N. M., Tešić, Ž. Lj., Milojkovic-Opsenica, D. M., Barac, M. B., Stanojević, S. P.,& Pesic, M. B.. (2017). Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 56(1), 13-20.
https://doi.org/10.1080/00218839.2016.1259897

Kostic AZ, Petrović TS, Krnjaja V, Nedic NM, Tešić ŽL, Milojkovic-Opsenica DM, Barac MB, Stanojević SP, Pesic MB. Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions. in Journal of Apicultural Research. 2017;56(1):13-20.
doi:10.1080/00218839.2016.1259897 .

Kostic, Aleksandar Z., Petrović, Tanja S., Krnjaja, Vesna, Nedic, Nebojša M., Tešić, Živoslav Lj., Milojkovic-Opsenica, Dusanka M., Barac, Miroljub B., Stanojević, Sladana P., Pesic, Mirjana B., "Mold/aflatoxin contamination of honey bee collected pollen from different Serbian regions" in Journal of Apicultural Research, 56, no. 1 (2017):13-20,
https://doi.org/10.1080/00218839.2016.1259897 . .

TY  - JOUR
AU  - Petrović, Tanja S.
AU  - Nedic, Nebojša M.
AU  - Paunović, D.
AU  - Rajić, J.
AU  - Matović, K.
AU  - Radulović, Z.
AU  - Krnjaja, Vesna
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/418
AB  - Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health.
AB  - Ukupan broj gljiva, učestalost (incidenca) gljiva i koncentracija aflatoksina B1 (AFB1) ispitivani su u 33 uzoraka polena sakupljenih od pčelara iz različitih regiona u Srbiji. Ukupan broj gljiva određen je primenom metode razređenja a AFB1 je određen primenom imunoadsorpcione enzimske metode (ELISA). Mikološkim ispitivanjima identifikovano je devet rodova gljiva: Acremonium, Alternaria, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Penicillium i Rhizopus, sa ukupnim brojem od 1 x 103 to 1 x 105 CFU g-1. Najučestalije vrste gljiva su u rodovima Aspergillus i Alternaria. Među Aspergillus vrstama najučestalija je vrsta A. flavus sa incidencom od 27,27%. AFB1 je detektovan u svim uzorcima sa prosečnom koncentracijom od 8,61 μg kg-1. Dobijeni rezultati ukazuju da pčelarski polen mora biti strogo kontrolisan tokom prikupljanja i njegove dalje prerade. Zbog toga, sprovođenje dobre proizvođačke (pčelarske) prakse podrazumeva definisanje procedura za pčelarske proizvode što bi moglo biti presudno za smanjenje rizika od moguće kontaminacije i dobijanje prirodnih i bezbednih proizvoda bez rizika po zdravlje ljudi.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia
T1  - Prirodna mikobiota i prisustvo aflatoksina B1 u polenu prikupljenom u Srbiji
VL  - 30
IS  - 4
SP  - 731
EP  - 741
DO  - 10.2298/BAH1404731P
ER  - 

@article{
author = "Petrović, Tanja S. and Nedic, Nebojša M. and Paunović, D. and Rajić, J. and Matović, K. and Radulović, Z. and Krnjaja, Vesna",
year = "2014",
abstract = "Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health., Ukupan broj gljiva, učestalost (incidenca) gljiva i koncentracija aflatoksina B1 (AFB1) ispitivani su u 33 uzoraka polena sakupljenih od pčelara iz različitih regiona u Srbiji. Ukupan broj gljiva određen je primenom metode razređenja a AFB1 je određen primenom imunoadsorpcione enzimske metode (ELISA). Mikološkim ispitivanjima identifikovano je devet rodova gljiva: Acremonium, Alternaria, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Penicillium i Rhizopus, sa ukupnim brojem od 1 x 103 to 1 x 105 CFU g-1. Najučestalije vrste gljiva su u rodovima Aspergillus i Alternaria. Među Aspergillus vrstama najučestalija je vrsta A. flavus sa incidencom od 27,27%. AFB1 je detektovan u svim uzorcima sa prosečnom koncentracijom od 8,61 μg kg-1. Dobijeni rezultati ukazuju da pčelarski polen mora biti strogo kontrolisan tokom prikupljanja i njegove dalje prerade. Zbog toga, sprovođenje dobre proizvođačke (pčelarske) prakse podrazumeva definisanje procedura za pčelarske proizvode što bi moglo biti presudno za smanjenje rizika od moguće kontaminacije i dobijanje prirodnih i bezbednih proizvoda bez rizika po zdravlje ljudi.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia, Prirodna mikobiota i prisustvo aflatoksina B1 u polenu prikupljenom u Srbiji",
volume = "30",
number = "4",
pages = "731-741",
doi = "10.2298/BAH1404731P"
}

Petrović, T. S., Nedic, N. M., Paunović, D., Rajić, J., Matović, K., Radulović, Z.,& Krnjaja, V.. (2014). Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 30(4), 731-741.
https://doi.org/10.2298/BAH1404731P

Petrović TS, Nedic NM, Paunović D, Rajić J, Matović K, Radulović Z, Krnjaja V. Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia. in Biotechnology in Animal Husbandry. 2014;30(4):731-741.
doi:10.2298/BAH1404731P .

Petrović, Tanja S., Nedic, Nebojša M., Paunović, D., Rajić, J., Matović, K., Radulović, Z., Krnjaja, Vesna, "Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia" in Biotechnology in Animal Husbandry, 30, no. 4 (2014):731-741,
https://doi.org/10.2298/BAH1404731P . .