TY  - CONF
AU  - Serafimov, Marios
AU  - Metodiev, Nikola
AU  - Kanzova, Hristina
AU  - Karadjova, Veronika
AU  - Cekić, Bogdan
AU  - Andreeva, Madlena
PY  - 2021-11
UR  - http://r.istocar.bg.ac.rs/handle/123456789/741
AB  - The aim of the present study was to determine a possible relationship between sperm
parameters and activity of creatinine kinase (CK) in the process of cryopreservation.
To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5
years, were examined. The rams were breeding under the same conditions of feeding,
growing and sexual use. Ejaculates were obtained via the artificial vagina method
and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined
by a computer assisted semen analysis (CASA) to determine total motility, velocity
parameters, and morphological status. The intracellular and extracellular activity of
CK were determined spectrophotometrically. After sperm evaluation, the ejaculates
were frozen using Cassou sequin technology and the same parameters were analyzed
after thawing the samples. From the obtained results we found a 26% decrease in
sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and
BCF also decreased significantly after thawing (P≤0.01), but an increase was found
in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation
also increased the percentage of abnormal sperm by 17%. A higher intracellular CK
activity, which decreased significantly after freeze-thawing (P≤0.001) was measured.
In conclusion, cryopreservation decreased sperm motility and increased the percentage
of abnormal sperm (mostly with tail damage), but we did not establish a relationship
between linear the velocity parameters LIN, STR and WOB, sperm morphological
status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm
quality in the process of cryopreservation.
PB  - Faculty of Biology, Sofia University, Bulgaria
C3  - Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria
T1  - Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation
SP  - 205
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_741
ER  - 

@conference{
author = "Serafimov, Marios and Metodiev, Nikola and Kanzova, Hristina and Karadjova, Veronika and Cekić, Bogdan and Andreeva, Madlena",
year = "2021-11",
abstract = "The aim of the present study was to determine a possible relationship between sperm
parameters and activity of creatinine kinase (CK) in the process of cryopreservation.
To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5
years, were examined. The rams were breeding under the same conditions of feeding,
growing and sexual use. Ejaculates were obtained via the artificial vagina method
and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined
by a computer assisted semen analysis (CASA) to determine total motility, velocity
parameters, and morphological status. The intracellular and extracellular activity of
CK were determined spectrophotometrically. After sperm evaluation, the ejaculates
were frozen using Cassou sequin technology and the same parameters were analyzed
after thawing the samples. From the obtained results we found a 26% decrease in
sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and
BCF also decreased significantly after thawing (P≤0.01), but an increase was found
in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation
also increased the percentage of abnormal sperm by 17%. A higher intracellular CK
activity, which decreased significantly after freeze-thawing (P≤0.001) was measured.
In conclusion, cryopreservation decreased sperm motility and increased the percentage
of abnormal sperm (mostly with tail damage), but we did not establish a relationship
between linear the velocity parameters LIN, STR and WOB, sperm morphological
status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm
quality in the process of cryopreservation.",
publisher = "Faculty of Biology, Sofia University, Bulgaria",
journal = "Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria",
title = "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation",
pages = "205-205",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_741"
}

Serafimov, M., Metodiev, N., Kanzova, H., Karadjova, V., Cekić, B.,& Andreeva, M.. (2021-11). Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria
Faculty of Biology, Sofia University, Bulgaria., 205-205.
https://hdl.handle.net/21.15107/rcub_ristocar_741

Serafimov M, Metodiev N, Kanzova H, Karadjova V, Cekić B, Andreeva M. Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria. 2021;:205-205.
https://hdl.handle.net/21.15107/rcub_ristocar_741 .

Serafimov, Marios, Metodiev, Nikola, Kanzova, Hristina, Karadjova, Veronika, Cekić, Bogdan, Andreeva, Madlena, "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation" in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria (2021-11):205-205,
https://hdl.handle.net/21.15107/rcub_ristocar_741 .