TY  - CONF
AU  - Serafimov, Marios
AU  - Metodiev, Nikola
AU  - Kanzova, Hristina
AU  - Karadjova, Veronika
AU  - Cekić, Bogdan
AU  - Andreeva, Madlena
PY  - 2021-11
UR  - http://r.istocar.bg.ac.rs/handle/123456789/741
AB  - The aim of the present study was to determine a possible relationship between sperm
parameters and activity of creatinine kinase (CK) in the process of cryopreservation.
To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5
years, were examined. The rams were breeding under the same conditions of feeding,
growing and sexual use. Ejaculates were obtained via the artificial vagina method
and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined
by a computer assisted semen analysis (CASA) to determine total motility, velocity
parameters, and morphological status. The intracellular and extracellular activity of
CK were determined spectrophotometrically. After sperm evaluation, the ejaculates
were frozen using Cassou sequin technology and the same parameters were analyzed
after thawing the samples. From the obtained results we found a 26% decrease in
sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and
BCF also decreased significantly after thawing (P≤0.01), but an increase was found
in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation
also increased the percentage of abnormal sperm by 17%. A higher intracellular CK
activity, which decreased significantly after freeze-thawing (P≤0.001) was measured.
In conclusion, cryopreservation decreased sperm motility and increased the percentage
of abnormal sperm (mostly with tail damage), but we did not establish a relationship
between linear the velocity parameters LIN, STR and WOB, sperm morphological
status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm
quality in the process of cryopreservation.
PB  - Faculty of Biology, Sofia University, Bulgaria
C3  - Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria
T1  - Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation
SP  - 205
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_741
ER  - 

@conference{
author = "Serafimov, Marios and Metodiev, Nikola and Kanzova, Hristina and Karadjova, Veronika and Cekić, Bogdan and Andreeva, Madlena",
year = "2021-11",
abstract = "The aim of the present study was to determine a possible relationship between sperm
parameters and activity of creatinine kinase (CK) in the process of cryopreservation.
To achieve this goal, 14 ejaculates of 7 rams of the breed Ile de France, aged 3-5
years, were examined. The rams were breeding under the same conditions of feeding,
growing and sexual use. Ejaculates were obtained via the artificial vagina method
and were diluted 1:12 with 6 A-G extender. The resulting ejaculates were examined
by a computer assisted semen analysis (CASA) to determine total motility, velocity
parameters, and morphological status. The intracellular and extracellular activity of
CK were determined spectrophotometrically. After sperm evaluation, the ejaculates
were frozen using Cassou sequin technology and the same parameters were analyzed
after thawing the samples. From the obtained results we found a 26% decrease in
sperm motility after thawing. The velocity parameters for VCL, VSL, VAP, AHL and
BCF also decreased significantly after thawing (P≤0.01), but an increase was found
in the linear velocity parameters - LIN, STR and WOB (P≤0.01). Cryopreservation
also increased the percentage of abnormal sperm by 17%. A higher intracellular CK
activity, which decreased significantly after freeze-thawing (P≤0.001) was measured.
In conclusion, cryopreservation decreased sperm motility and increased the percentage
of abnormal sperm (mostly with tail damage), but we did not establish a relationship
between linear the velocity parameters LIN, STR and WOB, sperm morphological
status and CK activity. Therefore, CK activity cannot serve as an indicator of sperm
quality in the process of cryopreservation.",
publisher = "Faculty of Biology, Sofia University, Bulgaria",
journal = "Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria",
title = "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation",
pages = "205-205",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_741"
}

Serafimov, M., Metodiev, N., Kanzova, H., Karadjova, V., Cekić, B.,& Andreeva, M.. (2021-11). Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria
Faculty of Biology, Sofia University, Bulgaria., 205-205.
https://hdl.handle.net/21.15107/rcub_ristocar_741

Serafimov M, Metodiev N, Kanzova H, Karadjova V, Cekić B, Andreeva M. Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation. in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria. 2021;:205-205.
https://hdl.handle.net/21.15107/rcub_ristocar_741 .

Serafimov, Marios, Metodiev, Nikola, Kanzova, Hristina, Karadjova, Veronika, Cekić, Bogdan, Andreeva, Madlena, "Study of the relationship between sperm parameters and the activity of creatine kinase in the process of cryopreservation" in Book of abstracts of Scientific conference "Kliment's days" , November 5th, 2021, Sofia, Bulgaria (2021-11):205-205,
https://hdl.handle.net/21.15107/rcub_ristocar_741 .

TY  - CONF
AU  - Andreeva, Madlena
AU  - Metodiev, Nikola
AU  - Cekić, Bogdan
AU  - Stefanov, Rossen
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/671
AB  - The aim  of  the  study  was  to  evaluate how  the  process  of  cryopreservation   influenced   the   morphological   status   of   ram   sperm.   The   experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed –   15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse  E200,  Japan)  by  staining  with  SpermBlue  (Microptic,  Spain),  performed according  to  the  manufacturer's  protocol,  and  observed  under  bright  field  with  magnification  of  ×40.  Cryopreservation  of  semen  straws  was  done  by  the  method  of  Cassou  (1964).  After  cryopreservation,  the  percentage  of  spermatozoa  with  normal  morphology  decreased  and  those  with  abnormal  morphology  increased.  This difference was more pronounced at rams from SPBM compared to SB rams. After  freezing,  the  percentage  of  spermatozoa  with  damage  to  the  head  at  rams  from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low  temperatures  of  cryopreservation  had a  detrimental  effect  to  the  rams’  sperm  structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings of the 12th International Symposium Modern Trends in Livestock Production
T1  - Study of the effects of low temperatures on the morphological status of ram spermatozoa
SP  - 373
EP  - 381
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_671
ER  - 

@conference{
author = "Andreeva, Madlena and Metodiev, Nikola and Cekić, Bogdan and Stefanov, Rossen",
year = "2019",
abstract = "The aim  of  the  study  was  to  evaluate how  the  process  of  cryopreservation   influenced   the   morphological   status   of   ram   sperm.   The   experiment was carried out with 26 sexually matured, pure bred rams during their breeding campaign. The animals were divided in two groups according to the breed –   15 rams from Synthetic Population of Bulgarian Milk (SPBM) and 11 rams from Sofia breed (SB). The sperm morphology was evaluated under microscope (Nikon Eclipse  E200,  Japan)  by  staining  with  SpermBlue  (Microptic,  Spain),  performed according  to  the  manufacturer's  protocol,  and  observed  under  bright  field  with  magnification  of  ×40.  Cryopreservation  of  semen  straws  was  done  by  the  method  of  Cassou  (1964).  After  cryopreservation,  the  percentage  of  spermatozoa  with  normal  morphology  decreased  and  those  with  abnormal  morphology  increased.  This difference was more pronounced at rams from SPBM compared to SB rams. After  freezing,  the  percentage  of  spermatozoa  with  damage  to  the  head  at  rams  from both breeds increased. The percentage of the spermatozoa with damage in the midpiece and the tail was higher and significant (P<0.001) for the SPBM rams. The low  temperatures  of  cryopreservation  had a  detrimental  effect  to  the  rams’  sperm  structures. This damaging effect was much stronger for the rams from SPBM breed compared to rams from Sofia breed.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings of the 12th International Symposium Modern Trends in Livestock Production",
title = "Study of the effects of low temperatures on the morphological status of ram spermatozoa",
pages = "373-381",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_671"
}

Andreeva, M., Metodiev, N., Cekić, B.,& Stefanov, R.. (2019). Study of the effects of low temperatures on the morphological status of ram spermatozoa. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production
Institute for Animal Husbandry, Belgrade-Zemun., 373-381.
https://hdl.handle.net/21.15107/rcub_ristocar_671

Andreeva M, Metodiev N, Cekić B, Stefanov R. Study of the effects of low temperatures on the morphological status of ram spermatozoa. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production. 2019;:373-381.
https://hdl.handle.net/21.15107/rcub_ristocar_671 .

Andreeva, Madlena, Metodiev, Nikola, Cekić, Bogdan, Stefanov, Rossen, "Study of the effects of low temperatures on the morphological status of ram spermatozoa" in Proceedings of the 12th International Symposium Modern Trends in Livestock Production (2019):373-381,
https://hdl.handle.net/21.15107/rcub_ristocar_671 .