TY  - JOUR
AU  - Mandić, Violeta
AU  - Krnjaja, vesna
AU  - Simić, Aleksandar
AU  - Petričević, Maja
AU  - Gogić, Marija
AU  - Brankov, Milan
AU  - Stanojković, Aleksandar
PY  - 2023-12
UR  - http://r.istocar.bg.ac.rs/handle/123456789/971
AB  - Soil pH is one of the most important limiting factors for crop cultivation, including maize. About 30-40% of the world's arable land is acidic, and over a billion ha is alkaline. Today, there are 205 million ha of arable land under maize in the world, so it ranks third in area after rice and wheat. Maize hybrids have wide genetic variability and high pH tolerance. We tested the tolerance of maize hybrids seedlings of ZP 4708 and ZP 5797 to pH 5, 6, 7, and 8. Results showed that hybrid ZP 4708 has a higher adaptability to low and high pH, due to the higher value of germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. Results further showed, on average for hybrids, that pH 5 and pH 8 significantly decreased the GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), shoot fresh weight (ShDW), and SVI. These parameters did not differ significantly between pH 6 and pH 7. Seed germination was not affected by the pH, indicating that the seed vigor of both hybrids supports full germination potential. However, it could be expected that exposure to the lowest and highest pH values of the medium could affect seedlings growth in the field conditions, even though germination is high. SVI showed a positive and significant correlation with all investigated parameters, except germination (G) and relative seedling water content (RSWC).
AB  - pH zemlji ta je jedan od va nih ograni avajućih faktora za gajenje useva, a samim tim i kukuruza. Oko 30-40% svetskih obradivih povr ina je kisele reakcije, a preko milijardu ha je alkalno. Danas je u svetu 205 miliona ha obradivih povr ina pod kukuruzom, tako da on zauzima treće mesto po povr ini posle pirin a i p enice. Hibridi kukuruza imaju iroku genetsku varijabilnost i toleranciju za pH. Ispitivali smo toleranciju klijanaca novih hibrida kukuruza ZP 4708 i ZP 5797 na pH 5, 6, 7 i 8. Rezultati su pokazali da hibrid ZP 4 08 ima bolju sposobnost adaptacije na nisku i visoku pH vrednost jer je imao veću energiju klijanja (EK), du inu stabla (DS), sve u masu stabla (SvMS), suvu masu stabla (SuMS), indeks klijavosti (IK) i vigor indeks (VI) od hibrida ZP 5797. Rezultati su dalje pokazali, u proseku za hibride, da su niski (pH 5) i visoki pH nivo medijuma (pH8) zna ajno smanjili EK, du inu korena (DK), DS, sve u masu korena (SvMK), SvMS, suvu masu korena (SuMK), SuMS i VI. Ovi parametri se nisu razlikovali između tretmana pH 6 i pH . pH medijuma nije uticala na klijanje semena to ukazuje da snaga semena oba hibrida podr ava pun potencijal klijanja. Međutim, izlaganje najni im i najvi im pH vrednostima medijuma je dovelo do toga da nije moguće o ekivati jake klijance, iako je klijavost visoka. VI je pokazao pozitivnu i zna ajnu korelaciju sa svim ispitivanim parametrima, osim sa klijanjem semena i relativnim sadr ajem vode u klijancima.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - Effect of pH on germination and seedling growth of maize.
T1  - Uticaj pH vrednosti na klijanje i rast klijanaca kukuruza
VL  - 39
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/BAH2302195M
ER  - 

@article{
author = "Mandić, Violeta and Krnjaja, vesna and Simić, Aleksandar and Petričević, Maja and Gogić, Marija and Brankov, Milan and Stanojković, Aleksandar",
year = "2023-12",
abstract = "Soil pH is one of the most important limiting factors for crop cultivation, including maize. About 30-40% of the world's arable land is acidic, and over a billion ha is alkaline. Today, there are 205 million ha of arable land under maize in the world, so it ranks third in area after rice and wheat. Maize hybrids have wide genetic variability and high pH tolerance. We tested the tolerance of maize hybrids seedlings of ZP 4708 and ZP 5797 to pH 5, 6, 7, and 8. Results showed that hybrid ZP 4708 has a higher adaptability to low and high pH, due to the higher value of germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. Results further showed, on average for hybrids, that pH 5 and pH 8 significantly decreased the GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), shoot fresh weight (ShDW), and SVI. These parameters did not differ significantly between pH 6 and pH 7. Seed germination was not affected by the pH, indicating that the seed vigor of both hybrids supports full germination potential. However, it could be expected that exposure to the lowest and highest pH values of the medium could affect seedlings growth in the field conditions, even though germination is high. SVI showed a positive and significant correlation with all investigated parameters, except germination (G) and relative seedling water content (RSWC)., pH zemlji ta je jedan od va nih ograni avajućih faktora za gajenje useva, a samim tim i kukuruza. Oko 30-40% svetskih obradivih povr ina je kisele reakcije, a preko milijardu ha je alkalno. Danas je u svetu 205 miliona ha obradivih povr ina pod kukuruzom, tako da on zauzima treće mesto po povr ini posle pirin a i p enice. Hibridi kukuruza imaju iroku genetsku varijabilnost i toleranciju za pH. Ispitivali smo toleranciju klijanaca novih hibrida kukuruza ZP 4708 i ZP 5797 na pH 5, 6, 7 i 8. Rezultati su pokazali da hibrid ZP 4 08 ima bolju sposobnost adaptacije na nisku i visoku pH vrednost jer je imao veću energiju klijanja (EK), du inu stabla (DS), sve u masu stabla (SvMS), suvu masu stabla (SuMS), indeks klijavosti (IK) i vigor indeks (VI) od hibrida ZP 5797. Rezultati su dalje pokazali, u proseku za hibride, da su niski (pH 5) i visoki pH nivo medijuma (pH8) zna ajno smanjili EK, du inu korena (DK), DS, sve u masu korena (SvMK), SvMS, suvu masu korena (SuMK), SuMS i VI. Ovi parametri se nisu razlikovali između tretmana pH 6 i pH . pH medijuma nije uticala na klijanje semena to ukazuje da snaga semena oba hibrida podr ava pun potencijal klijanja. Međutim, izlaganje najni im i najvi im pH vrednostima medijuma je dovelo do toga da nije moguće o ekivati jake klijance, iako je klijavost visoka. VI je pokazao pozitivnu i zna ajnu korelaciju sa svim ispitivanim parametrima, osim sa klijanjem semena i relativnim sadr ajem vode u klijancima.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Effect of pH on germination and seedling growth of maize., Uticaj pH vrednosti na klijanje i rast klijanaca kukuruza",
volume = "39",
number = "2",
pages = "195-203",
doi = "10.2298/BAH2302195M"
}

Mandić, V., Krnjaja, v., Simić, A., Petričević, M., Gogić, M., Brankov, M.,& Stanojković, A.. (2023-12). Effect of pH on germination and seedling growth of maize.. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 39(2), 195-203.
https://doi.org/10.2298/BAH2302195M

Mandić V, Krnjaja V, Simić A, Petričević M, Gogić M, Brankov M, Stanojković A. Effect of pH on germination and seedling growth of maize.. in Biotechnology in Animal Husbandry. 2023;39(2):195-203.
doi:10.2298/BAH2302195M .

Mandić, Violeta, Krnjaja, vesna, Simić, Aleksandar, Petričević, Maja, Gogić, Marija, Brankov, Milan, Stanojković, Aleksandar, "Effect of pH on germination and seedling growth of maize." in Biotechnology in Animal Husbandry, 39, no. 2 (2023-12):195-203,
https://doi.org/10.2298/BAH2302195M . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/904
AB  - Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_904
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_904"
}

Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904

Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):79-79,
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Caro Petrović, Violeta
AU  - Gogić, Marija
PY  - 2022-07-17
UR  - http://r.istocar.bg.ac.rs/handle/123456789/792
AB  - The main aim of this research was to study the effect of two sowing times (early and late) on Fusarium and fumonisin contamination and some yield component traits of two maize hybrids from the FAO maturity groups 500 (ZP 560) and 600 (ZP 666) within a two-year growing season (2016–2017). F. verticillioides and F. subglutinans have been identified as Fusarium ear rot (FER) pathogens and potential producers of B-type fumonisins (FBs), with F. verticillioides as the predominant Fusarium species in both years. The incidence of F. verticillioides and FB levels were affected by sowing time and maize hybridity. With early sowing and the mid-maturity hybrid ZP 560, F. verticillioides and FB contamination were lower than with late sowing and the late-maturity hybrid ZP 666. Yield parameters also differed significantly between sowing time and maize hybrid treatments. Early sowing increased ear length (EL), number of grains per ear (NGE), grain weight per ear (GWE), and grain yield per hectare (GY). The late-maturity hybrid ZP 666 had higher yield component traits and GY than the mid-maturity hybrid ZP 560. EL, GWE, thousand-grain weight (TGW), and GY were affected by year. Interactions between sowing times and maize hybrids were highly significant (p ≤ 0.01) for FB level, GWE, and GY. The obtained results indicate the importance of applying early sowing to achieve high maize grain yields with lower contamination by F. verticillioides and FBs. Although mid-maturity hybrid ZP 560 and late-maturity hybrid ZP 666 showed significant differences in terms of levels of F. verticillioides and FB contamination, both were susceptible to F. verticillioides, with high FB levels. These results should be useful to breeders of maize hybrids to create genotypes more resistant to these fungal contaminants.
T2  - Agriculture
T1  - Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits
VL  - 12
IS  - 7
SP  - 1042
DO  - https://doi.org/10.3390/agriculture12071042
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Caro Petrović, Violeta and Gogić, Marija",
year = "2022-07-17",
abstract = "The main aim of this research was to study the effect of two sowing times (early and late) on Fusarium and fumonisin contamination and some yield component traits of two maize hybrids from the FAO maturity groups 500 (ZP 560) and 600 (ZP 666) within a two-year growing season (2016–2017). F. verticillioides and F. subglutinans have been identified as Fusarium ear rot (FER) pathogens and potential producers of B-type fumonisins (FBs), with F. verticillioides as the predominant Fusarium species in both years. The incidence of F. verticillioides and FB levels were affected by sowing time and maize hybridity. With early sowing and the mid-maturity hybrid ZP 560, F. verticillioides and FB contamination were lower than with late sowing and the late-maturity hybrid ZP 666. Yield parameters also differed significantly between sowing time and maize hybrid treatments. Early sowing increased ear length (EL), number of grains per ear (NGE), grain weight per ear (GWE), and grain yield per hectare (GY). The late-maturity hybrid ZP 666 had higher yield component traits and GY than the mid-maturity hybrid ZP 560. EL, GWE, thousand-grain weight (TGW), and GY were affected by year. Interactions between sowing times and maize hybrids were highly significant (p ≤ 0.01) for FB level, GWE, and GY. The obtained results indicate the importance of applying early sowing to achieve high maize grain yields with lower contamination by F. verticillioides and FBs. Although mid-maturity hybrid ZP 560 and late-maturity hybrid ZP 666 showed significant differences in terms of levels of F. verticillioides and FB contamination, both were susceptible to F. verticillioides, with high FB levels. These results should be useful to breeders of maize hybrids to create genotypes more resistant to these fungal contaminants.",
journal = "Agriculture",
title = "Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits",
volume = "12",
number = "7",
pages = "1042",
doi = "https://doi.org/10.3390/agriculture12071042"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Caro Petrović, V.,& Gogić, M.. (2022-07-17). Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits. in Agriculture, 12(7), 1042.
https://doi.org/https://doi.org/10.3390/agriculture12071042

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Caro Petrović V, Gogić M. Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits. in Agriculture. 2022;12(7):1042.
doi:https://doi.org/10.3390/agriculture12071042 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Caro Petrović, Violeta, Gogić, Marija, "Influence of sowing time on Fusarium and fumonisin contamination of maize grains and yield component traits" in Agriculture, 12, no. 7 (2022-07-17):1042,
https://doi.org/https://doi.org/10.3390/agriculture12071042 . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Caro Petrović, Violeta
AU  - Ostojić Andrić, Dušica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/790
AB  - Fusarium head blight (FHB) is economically the most important wheat disease, 
causing yield and quality losses. Fungal species from the genus Fusarium are the 
main causative agents of FHB, with Fusarium graminearum as the predominant 
species. F. graminearum synthesizes more different secondary metabolites 
(mycotoxins). In wheat, the most studied mycotoxins are trichothecenes and 
zearalenone produced by F. graminearum. Trichothecene deoxynivalenol (DON) 
and its acetylated forms 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON) are the most detected in wheat grains. Strategies for 
controlling FHB and DON in wheat includе different preharvest and postharvest 
measures, emphasizing integrated approaches. The main aim of this review was 
to present some preharvest and postharvest strategies for integrated FHB 
management in wheat production.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Science
C3  - Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“
T1  - Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_790
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Caro Petrović, Violeta and Ostojić Andrić, Dušica",
year = "2022",
abstract = "Fusarium head blight (FHB) is economically the most important wheat disease, 
causing yield and quality losses. Fungal species from the genus Fusarium are the 
main causative agents of FHB, with Fusarium graminearum as the predominant 
species. F. graminearum synthesizes more different secondary metabolites 
(mycotoxins). In wheat, the most studied mycotoxins are trichothecenes and 
zearalenone produced by F. graminearum. Trichothecene deoxynivalenol (DON) 
and its acetylated forms 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON) are the most detected in wheat grains. Strategies for 
controlling FHB and DON in wheat includе different preharvest and postharvest 
measures, emphasizing integrated approaches. The main aim of this review was 
to present some preharvest and postharvest strategies for integrated FHB 
management in wheat production.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Science",
journal = "Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“",
title = "Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_790"
}

Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Caro Petrović, V.,& Ostojić Andrić, D.. (2022). Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat. in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“
The Balkans Scientific Center of the Russian Academy of Natural Science..
https://hdl.handle.net/21.15107/rcub_ristocar_790

Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Caro Petrović V, Ostojić Andrić D. Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat. in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“. 2022;.
https://hdl.handle.net/21.15107/rcub_ristocar_790 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Bijelić, Zorica, Caro Petrović, Violeta, Ostojić Andrić, Dušica, "Integrated strategies for managing Fusarium head blight and deoxynivalenol contamination in wheat" in Proceedings of the 4th International Symposium „Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection“ (2022),
https://hdl.handle.net/21.15107/rcub_ristocar_790 .

TY  - CONF
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Jauković, Marko
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/795
AB  - The species of the genus Fusarium and Aspergillus are the most common pathogens of maize 
kernels worldwide. The most common species among them are F. verticillioides, F. 
graminearum and A. flavus. These fungi produce a wide spectrum of mycotoxins, among which 
the most common are fusariotoxins: trichothecenes-deoxynivalenol (DON), fumonisins (FBs) 
and aflatoxins: aflatoxin B1 (AFB1). The aim of this study was to examine the mycopopulation 
on maize kernels after harvest, as well as the concentration of their mycotoxins in kernels.
Standard mycological examinations of maize kernels revealed the presence of toxigenic species 
of fungi from three genus, Fusarium, Aspergillus and Penicillium. In the examined samples, the 
species F. verticillioides was most often isolated in majority of hybrids, with a maximum 
incidence of 32%, while the presence of Aspergillus spp. was from 0 to 17%. Mycotoxicological 
analysis of maize kernels was performed by the ELISA method using a commercial kit (Tecna 
S.r.l., Italy). All analysed samples were positive for the presence of at least one mycotoxin. The 
differences between the examined hybrids in the concentration of mycotoxins in the grain were 
statistically significant (P <0.001) for DON and FBs, but not for the content of AFB1. Likewise, 
the interaction between hybrids and localities was statistically significant (P <0.001) for DON 
and FBS content, while it was not statistically significant for AFB1 concentration. The average 
DON concentrations were 127,55 µg kg–1
, FBs 3050,21 µg kg–1
, and AFB1 2,98 µg kg–1
.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Contamination of maize kernels with mycotoxins after harvest
SP  - 195
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_795
ER  - 

@conference{
author = "Obradović, Ana and Stanković, Slavica and Krnjaja, Vesna and Kostadinović, Marija and Ristić, Danijela and Jauković, Marko",
year = "2022",
abstract = "The species of the genus Fusarium and Aspergillus are the most common pathogens of maize 
kernels worldwide. The most common species among them are F. verticillioides, F. 
graminearum and A. flavus. These fungi produce a wide spectrum of mycotoxins, among which 
the most common are fusariotoxins: trichothecenes-deoxynivalenol (DON), fumonisins (FBs) 
and aflatoxins: aflatoxin B1 (AFB1). The aim of this study was to examine the mycopopulation 
on maize kernels after harvest, as well as the concentration of their mycotoxins in kernels.
Standard mycological examinations of maize kernels revealed the presence of toxigenic species 
of fungi from three genus, Fusarium, Aspergillus and Penicillium. In the examined samples, the 
species F. verticillioides was most often isolated in majority of hybrids, with a maximum 
incidence of 32%, while the presence of Aspergillus spp. was from 0 to 17%. Mycotoxicological 
analysis of maize kernels was performed by the ELISA method using a commercial kit (Tecna 
S.r.l., Italy). All analysed samples were positive for the presence of at least one mycotoxin. The 
differences between the examined hybrids in the concentration of mycotoxins in the grain were 
statistically significant (P <0.001) for DON and FBs, but not for the content of AFB1. Likewise, 
the interaction between hybrids and localities was statistically significant (P <0.001) for DON 
and FBS content, while it was not statistically significant for AFB1 concentration. The average 
DON concentrations were 127,55 µg kg–1
, FBs 3050,21 µg kg–1
, and AFB1 2,98 µg kg–1
.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Contamination of maize kernels with mycotoxins after harvest",
pages = "195-195",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_795"
}

Obradović, A., Stanković, S., Krnjaja, V., Kostadinović, M., Ristić, D.,& Jauković, M.. (2022). Contamination of maize kernels with mycotoxins after harvest. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 195-195.
https://hdl.handle.net/21.15107/rcub_ristocar_795

Obradović A, Stanković S, Krnjaja V, Kostadinović M, Ristić D, Jauković M. Contamination of maize kernels with mycotoxins after harvest. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:195-195.
https://hdl.handle.net/21.15107/rcub_ristocar_795 .

Obradović, Ana, Stanković, Slavica, Krnjaja, Vesna, Kostadinović, Marija, Ristić, Danijela, Jauković, Marko, "Contamination of maize kernels with mycotoxins after harvest" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):195-195,
https://hdl.handle.net/21.15107/rcub_ristocar_795 .

TY  - CONF
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Gogić, Marija
AU  - Petričević, Maja
AU  - Đorđević, Snežana
AU  - Simić, Aleksandar
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/816
AB  - The objective of this study was to evaluate the effect of applying lactic acid bacteria (LAB) and 
enzymes mixture inoculants (Sil-All and Silaprilis) on the chemical composition and 
fermentation of high-moisture grain silage of two maize hybrids Zenit and ZP 735. Maize 
hybrids were harvested at 68-72% of dry matter. Commercial inoculants were prepared and 
sprayed following the manufacturer's specifications. Silages were stored in glass jars with a 
special valve filled with water in the middle of the lid. Significant differences between hybrids 
were found for ash, crude protein, pH, and acetic acid. The hybrid Zenit had significantly higher 
ash (14.9 g kg-1
dry matter (DM)), pH (4.03), and acetic acid (6.3 g kg-1 DM), and significantly 
lower crude protein (89.0 g kg-1 DM) than hybrid ZP 735 (12.5 g kg-1 DM, 3.98, 5.1 g kg-1 DM 
and 101.2 g kg-1 DM, respectively). Compared to control, LAB+enzymes mixture inoculants 
stimulated ensiling of high-moisture maize grain. Inoculants decreased the contents of ammonia 
nitrogen and acetic acid, and pH value, and increased the contents of dry matter, ash, crude 
protein, crude fat, and lactic acid during silage fermentation than control. Accordingly, the 
application of LAB+enzymes mixture inoculants is justified and they can be recommended for 
high-quality silage production in feeding livestock.
PB  - Faculty of agriculture, Sarajevo
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage
SP  - 178
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_816
ER  - 

@conference{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Gogić, Marija and Petričević, Maja and Đorđević, Snežana and Simić, Aleksandar",
year = "2022",
abstract = "The objective of this study was to evaluate the effect of applying lactic acid bacteria (LAB) and 
enzymes mixture inoculants (Sil-All and Silaprilis) on the chemical composition and 
fermentation of high-moisture grain silage of two maize hybrids Zenit and ZP 735. Maize 
hybrids were harvested at 68-72% of dry matter. Commercial inoculants were prepared and 
sprayed following the manufacturer's specifications. Silages were stored in glass jars with a 
special valve filled with water in the middle of the lid. Significant differences between hybrids 
were found for ash, crude protein, pH, and acetic acid. The hybrid Zenit had significantly higher 
ash (14.9 g kg-1
dry matter (DM)), pH (4.03), and acetic acid (6.3 g kg-1 DM), and significantly 
lower crude protein (89.0 g kg-1 DM) than hybrid ZP 735 (12.5 g kg-1 DM, 3.98, 5.1 g kg-1 DM 
and 101.2 g kg-1 DM, respectively). Compared to control, LAB+enzymes mixture inoculants 
stimulated ensiling of high-moisture maize grain. Inoculants decreased the contents of ammonia 
nitrogen and acetic acid, and pH value, and increased the contents of dry matter, ash, crude 
protein, crude fat, and lactic acid during silage fermentation than control. Accordingly, the 
application of LAB+enzymes mixture inoculants is justified and they can be recommended for 
high-quality silage production in feeding livestock.",
publisher = "Faculty of agriculture, Sarajevo",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage",
pages = "178-184",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_816"
}

Mandić, V., Bijelić, Z., Krnjaja, V., Gogić, M., Petričević, M., Đorđević, S.,& Simić, A.. (2022). Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
Faculty of agriculture, Sarajevo., 178-184.
https://hdl.handle.net/21.15107/rcub_ristocar_816

Mandić V, Bijelić Z, Krnjaja V, Gogić M, Petričević M, Đorđević S, Simić A. Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;:178-184.
https://hdl.handle.net/21.15107/rcub_ristocar_816 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Gogić, Marija, Petričević, Maja, Đorđević, Snežana, Simić, Aleksandar, "Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022):178-184,
https://hdl.handle.net/21.15107/rcub_ristocar_816 .

TY  - CONF
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Gogić, Marija
AU  - Petričević, Maja
AU  - Đorđević, Snežana
AU  - Simić, Aleksandar
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/796
AB  - The objective of this study was to evaluate the effect of applying lactic acid bacteria (LAB) and 
enzymes mixture inoculants (Sil-All and Silaprilis) on the chemical composition and 
fermentation of high-moisture grain silage of two maize hybrids Zenit and ZP 735. Maize 
hybrids were harvested at 68-72% of dry matter. Commercial inoculants were prepared and 
sprayed following the manufacturer's specifications. Silages were stored in glass jars with a 
special valve filled with water in the middle of the lid. Significant differences between hybrids 
were found for ash, crude protein, pH, and acetic acid. The hybrid Zenit had significantly higher 
ash (14.9 g kg-1
dry matter (DM)), pH (4.03), and acetic acid (6.3 g kg-1 DM), and significantly 
lower crude protein (89.0 g kg-1 DM) than hybrid ZP 735 (12.5 g kg-1 DM, 3.98, 5.1 g kg-1 DM 
and 101.2 g kg-1 DM, respectively). Compared to control, LAB+enzymes mixture inoculants 
stimulated ensiling of high-moisture maize grain. Inoculants decreased the contents of ammonia 
nitrogen and acetic acid, and pH value, and increased the contents of dry matter, ash, crude 
protein, crude fat, and lactic acid during silage fermentation than control. Accordingly, the 
application of LAB+enzymes mixture inoculants is justified and they can be recommended for 
high-quality silage production in feeding livestock.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage
SP  - 243
EP  - 243
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_796
ER  - 

@conference{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Gogić, Marija and Petričević, Maja and Đorđević, Snežana and Simić, Aleksandar",
year = "2022",
abstract = "The objective of this study was to evaluate the effect of applying lactic acid bacteria (LAB) and 
enzymes mixture inoculants (Sil-All and Silaprilis) on the chemical composition and 
fermentation of high-moisture grain silage of two maize hybrids Zenit and ZP 735. Maize 
hybrids were harvested at 68-72% of dry matter. Commercial inoculants were prepared and 
sprayed following the manufacturer's specifications. Silages were stored in glass jars with a 
special valve filled with water in the middle of the lid. Significant differences between hybrids 
were found for ash, crude protein, pH, and acetic acid. The hybrid Zenit had significantly higher 
ash (14.9 g kg-1
dry matter (DM)), pH (4.03), and acetic acid (6.3 g kg-1 DM), and significantly 
lower crude protein (89.0 g kg-1 DM) than hybrid ZP 735 (12.5 g kg-1 DM, 3.98, 5.1 g kg-1 DM 
and 101.2 g kg-1 DM, respectively). Compared to control, LAB+enzymes mixture inoculants 
stimulated ensiling of high-moisture maize grain. Inoculants decreased the contents of ammonia 
nitrogen and acetic acid, and pH value, and increased the contents of dry matter, ash, crude 
protein, crude fat, and lactic acid during silage fermentation than control. Accordingly, the 
application of LAB+enzymes mixture inoculants is justified and they can be recommended for 
high-quality silage production in feeding livestock.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage",
pages = "243-243",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_796"
}

Mandić, V., Bijelić, Z., Krnjaja, V., Gogić, M., Petričević, M., Đorđević, S.,& Simić, A.. (2022). Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 243-243.
https://hdl.handle.net/21.15107/rcub_ristocar_796

Mandić V, Bijelić Z, Krnjaja V, Gogić M, Petričević M, Đorđević S, Simić A. Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:243-243.
https://hdl.handle.net/21.15107/rcub_ristocar_796 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Gogić, Marija, Petričević, Maja, Đorđević, Snežana, Simić, Aleksandar, "Effects of bacteria and enzyme mixture inoculants on quality of high-moisture maize grain silage" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):243-243,
https://hdl.handle.net/21.15107/rcub_ristocar_796 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/801
AB  - Fusarium infection and deoxynivalenol (DON) contamination in seven 
durum wheat lines kernel (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, 
ZP DSP 66, and one international durum line Cimmyt 7817) during two harvest seasons 
(2015–2016) has been studied. The four Fusarium species, F. graminearum, F. proliferatum, 
F. sporotrichioides, and F. verticillioides, were identified in 2015. A different structure of 
the Fusarium population, which in addition to F. graminearum, F. sporotrichioides and F. 
verticillioides, also comprised F. poae, F. semitectum, and F. subglutinans, was identified 
in 2016. F. graminearum was the predominant species in the durum wheat lines kernels and 
the potential producer of DON. The other Fusarium spp. were isolated sporadically and with 
a low incidence in the kernels. The incidence of F. graminearum and DON levels were 
significantly affected by the wheat genotypes and studied years and these parameters were 
negatively correlated. The incidence of F. graminearum was significantly higher in 2015 
(75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 2016 
(3.636 mg kg-1) compared to 2015 (1.126 mg kg-1). Statistically, there was a significantly 
higher incidence of F. graminearum in ZP DSP 66 (73.00%) and ZP 120 (72.75%) durum 
wheat lines than in the other durum genotypes. DON level was the highest in durum wheat 
line ZP 120 (3.854 mg kg-1). Considering all treatments tested, the mean DON level was 
2.381 mg kg-1, while the mean incidence of F. graminearum was 69.64%. Тested durum 
wheat lines showed susceptibility to F. graminearum, resulting in high DON levels in kernels. 
The results obtained suggest the importance of using the lines with improved resistance to 
Fusarium head blight in the breeding programs for new durum wheat cultivars.
T2  - Matica Srpska Journal for Natural Sciences
T1  - Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol
VL  - 
IS  - 143
SP  - 27
EP  - 38
DO  - 10.2298/ZMSPN2243027K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Savić, Iva and Mandić, Violeta and Bijelić, Zorica",
year = "2022",
abstract = "Fusarium infection and deoxynivalenol (DON) contamination in seven 
durum wheat lines kernel (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, 
ZP DSP 66, and one international durum line Cimmyt 7817) during two harvest seasons 
(2015–2016) has been studied. The four Fusarium species, F. graminearum, F. proliferatum, 
F. sporotrichioides, and F. verticillioides, were identified in 2015. A different structure of 
the Fusarium population, which in addition to F. graminearum, F. sporotrichioides and F. 
verticillioides, also comprised F. poae, F. semitectum, and F. subglutinans, was identified 
in 2016. F. graminearum was the predominant species in the durum wheat lines kernels and 
the potential producer of DON. The other Fusarium spp. were isolated sporadically and with 
a low incidence in the kernels. The incidence of F. graminearum and DON levels were 
significantly affected by the wheat genotypes and studied years and these parameters were 
negatively correlated. The incidence of F. graminearum was significantly higher in 2015 
(75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 2016 
(3.636 mg kg-1) compared to 2015 (1.126 mg kg-1). Statistically, there was a significantly 
higher incidence of F. graminearum in ZP DSP 66 (73.00%) and ZP 120 (72.75%) durum 
wheat lines than in the other durum genotypes. DON level was the highest in durum wheat 
line ZP 120 (3.854 mg kg-1). Considering all treatments tested, the mean DON level was 
2.381 mg kg-1, while the mean incidence of F. graminearum was 69.64%. Тested durum 
wheat lines showed susceptibility to F. graminearum, resulting in high DON levels in kernels. 
The results obtained suggest the importance of using the lines with improved resistance to 
Fusarium head blight in the breeding programs for new durum wheat cultivars.",
journal = "Matica Srpska Journal for Natural Sciences",
title = "Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol",
volume = "",
number = "143",
pages = "27-38",
doi = "10.2298/ZMSPN2243027K"
}

Krnjaja, V., Stanković, S., Obradović, A., Nikolić, M., Savić, I., Mandić, V.,& Bijelić, Z.. (2022). Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol. in Matica Srpska Journal for Natural Sciences, (143), 27-38.
https://doi.org/10.2298/ZMSPN2243027K

Krnjaja V, Stanković S, Obradović A, Nikolić M, Savić I, Mandić V, Bijelić Z. Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol. in Matica Srpska Journal for Natural Sciences. 2022;(143):27-38.
doi:10.2298/ZMSPN2243027K .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Savić, Iva, Mandić, Violeta, Bijelić, Zorica, "Contamination of durum wheat lines kernels with Fusarium species and deoxynivalenol" in Matica Srpska Journal for Natural Sciences, , no. 143 (2022):27-38,
https://doi.org/10.2298/ZMSPN2243027K . .

TY  - CONF
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/803
AB  - Fusarium and Aspergillus toxigenic species are considered to be the most common pathogens 
in maize. Agroecological conditions in Serbia favour the development of numerous pathogenic 
and toxigenic species of the genus Fusarium and Aspergillus. Global climate change may 
favour the contamination of maize with high levels of aflatoxins and fusariotoxins in temperate 
areas, such as Serbia. Fusarium graminearum synthesises mycotoxins from which the most 
important are type B trichothecene - deoxynivalenol (DON), its acetyl-ester derivatives (3-
АDON and 15-АDON) and nivalenol (NIV). The two most important species of fungi 
responsible for aspergillosis kernel and ear rot are Aspergillus flavus and Aspergillus 
parasiticus, producers of aflatoxins (B1, B2, G1, and G2). 
Isolates of F. graminearum and A. parasiticus identified from maize grain samples collected 
over 12 years from 40 localities in Serbia were analyzed. Production potential of isolates were 
determined by high-performance liquid chromatography method (HPLC).
Results of perennial research showed high variability in the concentrations of mycotoxins 
deoxynivalenol and aflatoxins in maize kernels. Detected concentrations of DON varied from 
10,9 to 154.6 µg/g (average 47,2 µg/g), while 3ADON concentrations ranged from 3.42 to 71.6 
µg/g (average 15,6 µg/g ) and 15ADON from 5.4 to 121.3 µg/g (average 29,8 µg/g). Mycotoxin 
15ADON were predominant in observed samples. The average synthesis of aflatoxin B1 was 
4145.97 µg/kg (range 14.24 to 7361.03 µg/kg) by which the isolates of this group were 
classified into strong producers of AFB1. The average synthesis of aflatoxin B2 was 403.55 
µg/kg, aflatoxin G1 3490.07 µg/kg and G2 198.29 µg/kg averaging, respectivel
C3  - Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia
T1  - Toxicological profile pathogenic species on maize in Serbia
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_803
ER  - 

@conference{
author = "Obradović, Ana and Nikolić, Milica and Savić, Iva and Krnjaja, Vesna and Stanković, Slavica",
year = "2022",
abstract = "Fusarium and Aspergillus toxigenic species are considered to be the most common pathogens 
in maize. Agroecological conditions in Serbia favour the development of numerous pathogenic 
and toxigenic species of the genus Fusarium and Aspergillus. Global climate change may 
favour the contamination of maize with high levels of aflatoxins and fusariotoxins in temperate 
areas, such as Serbia. Fusarium graminearum synthesises mycotoxins from which the most 
important are type B trichothecene - deoxynivalenol (DON), its acetyl-ester derivatives (3-
АDON and 15-АDON) and nivalenol (NIV). The two most important species of fungi 
responsible for aspergillosis kernel and ear rot are Aspergillus flavus and Aspergillus 
parasiticus, producers of aflatoxins (B1, B2, G1, and G2). 
Isolates of F. graminearum and A. parasiticus identified from maize grain samples collected 
over 12 years from 40 localities in Serbia were analyzed. Production potential of isolates were 
determined by high-performance liquid chromatography method (HPLC).
Results of perennial research showed high variability in the concentrations of mycotoxins 
deoxynivalenol and aflatoxins in maize kernels. Detected concentrations of DON varied from 
10,9 to 154.6 µg/g (average 47,2 µg/g), while 3ADON concentrations ranged from 3.42 to 71.6 
µg/g (average 15,6 µg/g ) and 15ADON from 5.4 to 121.3 µg/g (average 29,8 µg/g). Mycotoxin 
15ADON were predominant in observed samples. The average synthesis of aflatoxin B1 was 
4145.97 µg/kg (range 14.24 to 7361.03 µg/kg) by which the isolates of this group were 
classified into strong producers of AFB1. The average synthesis of aflatoxin B2 was 403.55 
µg/kg, aflatoxin G1 3490.07 µg/kg and G2 198.29 µg/kg averaging, respectivel",
journal = "Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia",
title = "Toxicological profile pathogenic species on maize in Serbia",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_803"
}

Obradović, A., Nikolić, M., Savić, I., Krnjaja, V.,& Stanković, S.. (2022). Toxicological profile pathogenic species on maize in Serbia. in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia, 58-58.
https://hdl.handle.net/21.15107/rcub_ristocar_803

Obradović A, Nikolić M, Savić I, Krnjaja V, Stanković S. Toxicological profile pathogenic species on maize in Serbia. in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia. 2022;:58-58.
https://hdl.handle.net/21.15107/rcub_ristocar_803 .

Obradović, Ana, Nikolić, Milica, Savić, Iva, Krnjaja, Vesna, Stanković, Slavica, "Toxicological profile pathogenic species on maize in Serbia" in Book of Abstracts, XXVth EUCARPIA Maize and Sorghum  "Conference Current Challenges and New Methods for Maize and Sorghum Breeding", May 30 – June 2, 2022, Belgrade, Serbia (2022):58-58,
https://hdl.handle.net/21.15107/rcub_ristocar_803 .

TY  - CONF
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/802
AB  - Pšenica je na prvom mestu po površinama na kojima se gaji u svetu i značaju u 
prehrambenoj industriji. Različiti načini upotrebe zrna i suve mase žita u ishrani ljudi i 
životinja, kao i u industrijskoj preradi, ukazuju na to da ona imaju veliku upotrebnu vrednost. 
Zrno pšenice u različitim agroekološkim uslovima može biti zaraženo brojnim vrstama gljiva, 
kako u polju, tako i tokom čuvanja u skladištima. Vrste roda Fusarium su najčešći patogeni 
zrna pšenice, koji su posebno značajni, jer imaju sposobnost da sintetišu veliki spektar 
mikotoksina od kojih su najznačajniji fumonizin B1 (FB1), deoksinivalenol (DON) i zearalenon 
(ZEA). Cilj ovog rada bio je da se ispita koncentracija pomenutih mikotoksina u zrnu pšenice 
nakon žetve. 
Analizirano je 20 uzoraka zrna pšenice prikupljene iz različitih agroekoloških uslova u 
Srbiji. Pet grama od svakog osušenog i samlevenog uzorka je ekstrahovano i filtrirano 
rastvorom metanola i vode. Koncentracija mikotoksina je određena pomoću ELISA testa 
prema upustvu proizvođača (Tecna S.r.l., Italy, Celer Fumo Test Kit, Celer DON Gold Test Kit i 
Celer Zon Test Kit). U svim analiziranim uzorcima utvrđeno je prisustvo bar jednog 
ispitivanog mikotoksina. Ustanovljena koncentracija DON iznosila je od 0,596 do 2,258 µg/g 
(prosek 1,152 µg/g), FB1 od 0,852 do 6,253 µg/g (prosek 2,021 µg/кg) dok je mikotoksin ZEA 
bio prisutan u intervalu od 8,42 do 58,25 µg/кg (prosek 22,98 µg/кg). Utvrđene koncentracije 
ZEA i DON su bile niže od maksimalno dozvoljenih koncentracija koje su propisane 
Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani 
i hrani za životinje (Službeni glasnik Republike Srbije, 2019), dok su u pet uzoraka FB1
detektovani u koncentraciji većoj od dozvoljenih u ljudskoj hrani.
C3  - Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine
T1  - Fuzariotoksini na zrnu pšenice
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_802
ER  - 

@conference{
author = "Obradović, Ana and Stanković, Slavica and Krnjaja, Vesna and Nikolić, Milica and Savić, Iva and Stevanović, Milan and Stanković, Goran",
year = "2022",
abstract = "Pšenica je na prvom mestu po površinama na kojima se gaji u svetu i značaju u 
prehrambenoj industriji. Različiti načini upotrebe zrna i suve mase žita u ishrani ljudi i 
životinja, kao i u industrijskoj preradi, ukazuju na to da ona imaju veliku upotrebnu vrednost. 
Zrno pšenice u različitim agroekološkim uslovima može biti zaraženo brojnim vrstama gljiva, 
kako u polju, tako i tokom čuvanja u skladištima. Vrste roda Fusarium su najčešći patogeni 
zrna pšenice, koji su posebno značajni, jer imaju sposobnost da sintetišu veliki spektar 
mikotoksina od kojih su najznačajniji fumonizin B1 (FB1), deoksinivalenol (DON) i zearalenon 
(ZEA). Cilj ovog rada bio je da se ispita koncentracija pomenutih mikotoksina u zrnu pšenice 
nakon žetve. 
Analizirano je 20 uzoraka zrna pšenice prikupljene iz različitih agroekoloških uslova u 
Srbiji. Pet grama od svakog osušenog i samlevenog uzorka je ekstrahovano i filtrirano 
rastvorom metanola i vode. Koncentracija mikotoksina je određena pomoću ELISA testa 
prema upustvu proizvođača (Tecna S.r.l., Italy, Celer Fumo Test Kit, Celer DON Gold Test Kit i 
Celer Zon Test Kit). U svim analiziranim uzorcima utvrđeno je prisustvo bar jednog 
ispitivanog mikotoksina. Ustanovljena koncentracija DON iznosila je od 0,596 do 2,258 µg/g 
(prosek 1,152 µg/g), FB1 od 0,852 do 6,253 µg/g (prosek 2,021 µg/кg) dok je mikotoksin ZEA 
bio prisutan u intervalu od 8,42 do 58,25 µg/кg (prosek 22,98 µg/кg). Utvrđene koncentracije 
ZEA i DON su bile niže od maksimalno dozvoljenih koncentracija koje su propisane 
Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani 
i hrani za životinje (Službeni glasnik Republike Srbije, 2019), dok su u pet uzoraka FB1
detektovani u koncentraciji većoj od dozvoljenih u ljudskoj hrani.",
journal = "Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine",
title = "Fuzariotoksini na zrnu pšenice",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_802"
}

Obradović, A., Stanković, S., Krnjaja, V., Nikolić, M., Savić, I., Stevanović, M.,& Stanković, G.. (2022). Fuzariotoksini na zrnu pšenice. in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine, 48-48.
https://hdl.handle.net/21.15107/rcub_ristocar_802

Obradović A, Stanković S, Krnjaja V, Nikolić M, Savić I, Stevanović M, Stanković G. Fuzariotoksini na zrnu pšenice. in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine. 2022;:48-48.
https://hdl.handle.net/21.15107/rcub_ristocar_802 .

Obradović, Ana, Stanković, Slavica, Krnjaja, Vesna, Nikolić, Milica, Savić, Iva, Stevanović, Milan, Stanković, Goran, "Fuzariotoksini na zrnu pšenice" in Zbornik rezimea radova "XVII Savetovanje o zaštiti bilja", Zlatibor, 28.novembar - 1. decembar 2022. godine (2022):48-48,
https://hdl.handle.net/21.15107/rcub_ristocar_802 .

TY  - CONF
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Krnjaja, Vesna
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/794
AB  - In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Novel toxigenic species on maize kernels in Southeastern Europe
SP  - 194
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_794
ER  - 

@conference{
author = "Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Nikolić, Ana and Krnjaja, Vesna",
year = "2022",
abstract = "In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Novel toxigenic species on maize kernels in Southeastern Europe",
pages = "194-194",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_794"
}

Stanković, S., Obradović, A., Nikolić, M., Nikolić, A.,& Krnjaja, V.. (2022). Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794

Stanković S, Obradović A, Nikolić M, Nikolić A, Krnjaja V. Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794 .

Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Nikolić, Ana, Krnjaja, Vesna, "Novel toxigenic species on maize kernels in Southeastern Europe" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):194-194,
https://hdl.handle.net/21.15107/rcub_ristocar_794 .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/793
AB  - Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.
C3  - Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia
T1  - Fusarium and deoxynivalenol contamination of durum wheat lines kernels
SP  - 17
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_793
ER  - 

@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Savić, Iva and Mandić, Violeta and Bijelić, Zorica",
year = "2022",
abstract = "Fusarium infection and deoxynivalenol (DON) contamination on the kernels of seven durum 
wheat lines (six domestic durum lines ZP 16, ZP 34, ZP 41, ZP 74, ZP 120, ZP DSP 66, and 
one international durum line Cimmyt 7817) during harvest in two growing seasons (2015-
2016) have estimated. Mycological methods were performed to determine the incidence of 
Fusarium spp., while the Enzyme-Linked Immunosorbent Assay (ELISA) was used to 
quantify the total level of DON. Analysis of data was done by statistical method ANOVA 
(analysis of variance). Tukey’s test was used to compare means at a significance level of 5%. 
Correlation analyses were performed by Pearson’s test.
Based on morphological characteristics, four Fusarium species, F. graminearum, F. 
proliferatum, F. sporotrichioides, and F. verticillioides, were identified in 2015. A different 
structure of the Fusarium population, which in addition to F. graminearum, F. 
sporotrichioides and F. verticillioides, also consisted of F. poae, F. semitectum, and F. 
subglutinans, was identified in 2016. F. graminearum was the predominant species and the 
most common cause of Fusarium head blight (FHB) and the primary producer of DON. Other 
Fusarium spp. Were isolated sporadically and in a low incidence in the kernels. Fungal 
species from the genera Alternaria, Aspergillus, Chaetomium, Epicoccum, Nigrospora, and 
Penicillium have also been isolated.
The incidence of F. graminearum and level of DON were significantly affected by the wheat 
genotypes and investigated years. However, obtained results for these parameters were 
negatively correlated. Thus, in 2015, the incidence of F. graminearum was significantly 
higher (75.86%) than in 2016 (63.43%), while the level of DON was significantly higher in 
2016 (3.636 mg/kg) compared to 2015 (1.126 mg/kg). The highest and the lowest incidence 
of F. graminearum was on the kernels of line ZP DSP 66 (73%) and line ZP 34 (64.50%), 
respectively. The highest DON level was 3.854 mg/kg (line ZP 120), and the lowest was 
1.658 mg/kg (line ZP 41). The mean DON level was 2.381 mg/kg for all tested treatments 
and was above the maximum limit of 1.750 mg/kg prescribed by the European Regulation 
1881/2006/EC for unprocessed durum wheat, while the mean incidence of F. graminearum
was 69.64%.
Based on obtained results, tested durum wheat lines showed susceptibility to F. graminearum
and as a consequence higher accumulation of mycotoxin DON. These results indicate the 
importance of using less susceptible or tolerant lines to the pathogens of FHB and DON 
accumulation in the selection programs of new durum wheat varieties.",
journal = "Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia",
title = "Fusarium and deoxynivalenol contamination of durum wheat lines kernels",
pages = "17-17",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_793"
}

Krnjaja, V., Stanković, S., Obradović, A., Nikolić, M., Savić, I., Mandić, V.,& Bijelić, Z.. (2022). Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia, 17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793

Krnjaja V, Stanković S, Obradović A, Nikolić M, Savić I, Mandić V, Bijelić Z. Fusarium and deoxynivalenol contamination of durum wheat lines kernels. in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia. 2022;:17-17.
https://hdl.handle.net/21.15107/rcub_ristocar_793 .

Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Savić, Iva, Mandić, Violeta, Bijelić, Zorica, "Fusarium and deoxynivalenol contamination of durum wheat lines kernels" in Book of abstracts of the 7th International Scientific Meeting "Mycology, Mycotoxicology, and Mycoses", 2 – 3 June 2022,  Matica Srpska, Novi Sad, Serbia (2022):17-17,
https://hdl.handle.net/21.15107/rcub_ristocar_793 .

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/785
AB  - The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (genealogical concordance phylogenetic species recognition) revealed the existence of 15 phylogenetic species within what is now recognized as the Fusarium graminearum species complex (FGSC) (Sarver et al. 2011). During 1996–2008, a collection of FGSC isolates was established at the Maize Research Institute, Zemun Polje, and isolates originating from wheat (5), maize (3), and barley (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5 to 53.1 × 4.6 to 5.4 µm, n = 50) of all 10 isolates on potato dextrose agar (PDA) were consistent with descriptions of F. graminearum (Leslie and Summerell 2006; O’Donnell et al. 2004). Total DNA was isolated from mycelium removed from 7-day-old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3, and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b, and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in GenBank under accession numbers MF974399–MF974408 (TEF-1α), MG063783–MG063792 (β-tubulin), and MF999139–MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006) shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728), and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99 to 100% nucleotide similarity with F. graminearum NRRL 28439 (O’Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterházy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day-old colonies on PDA, and 30 ml of a conidial suspension (1 × 105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1 to 7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4–5.1 for F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON, and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Tóth et al. 2005) and in Korea on barley, corn, and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness, and toxicological profile of F. vorosii.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia
VL  - 106
IS  - 2
SP  - 758
EP  - 758
DO  - https://doi.org/10.1094/PDIS-04-21-0715-PDN
ER  - 

@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2022",
abstract = "The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (genealogical concordance phylogenetic species recognition) revealed the existence of 15 phylogenetic species within what is now recognized as the Fusarium graminearum species complex (FGSC) (Sarver et al. 2011). During 1996–2008, a collection of FGSC isolates was established at the Maize Research Institute, Zemun Polje, and isolates originating from wheat (5), maize (3), and barley (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5 to 53.1 × 4.6 to 5.4 µm, n = 50) of all 10 isolates on potato dextrose agar (PDA) were consistent with descriptions of F. graminearum (Leslie and Summerell 2006; O’Donnell et al. 2004). Total DNA was isolated from mycelium removed from 7-day-old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3, and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b, and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in GenBank under accession numbers MF974399–MF974408 (TEF-1α), MG063783–MG063792 (β-tubulin), and MF999139–MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006) shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728), and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99 to 100% nucleotide similarity with F. graminearum NRRL 28439 (O’Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterházy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day-old colonies on PDA, and 30 ml of a conidial suspension (1 × 105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1 to 7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4–5.1 for F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON, and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Tóth et al. 2005) and in Korea on barley, corn, and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness, and toxicological profile of F. vorosii.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia",
volume = "106",
number = "2",
pages = "758-758",
doi = "https://doi.org/10.1094/PDIS-04-21-0715-PDN"
}

Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2022). First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease
The American Phytopathological Society., 106(2), 758-758.
https://doi.org/https://doi.org/10.1094/PDIS-04-21-0715-PDN

Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease. 2022;106(2):758-758.
doi:https://doi.org/10.1094/PDIS-04-21-0715-PDN .

Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia" in Plant Disease, 106, no. 2 (2022):758-758,
https://doi.org/https://doi.org/10.1094/PDIS-04-21-0715-PDN . .

TY  - CONF
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Simić, Alesksandar
AU  - Gogić, Marija
AU  - Petričević, Maja
PY  - 2021-10
UR  - http://r.istocar.bg.ac.rs/handle/123456789/768
AB  - Maize is а highly productive crop intended for human and
animal nutrition, and industrial processing. The standard agrotechnical measures
are used in its production. However, the utilization of bacterial inoculants is
becoming more popular due to their effect on increased soil quality, plant
performance, and yield while preserving and enhancing the agroecosystem. In
addition, bacterial inoculants can be applied together with pesticides used in seed
treatments. Our earlier results showed that the inoculation of maize seed with
plant-growth-promoting rhizobacteria (PGPR) increased the number of diazotrophs
bacteria in the rhizosphere, morphological and productive traits, and yield of
maize. Accordingly, seed inoculation may represent an important strategy to
improve commercial maize production and reduce the environmental impact of
maize production processes.
AB  - Kukuruz je visokoproduktivni usev namenjen za ishranu ljudi i životinja i
industrijsku preradu. U njegovoj proizvodnji koriste se standardne agrotehničke
mere. Međutim, upotreba bakterijskih inokulanata postaje sve popularnija zbog
povećanja kvaliteta zemljišta, performansi biljaka i prinosa. Pored toga, bakterijski
inokulanti se mogu primeniti zajedno sa pesticidima koji se koriste u tretiranju
semena. Naši raniji rezultati pokazali su da inokulacija semena kukuruza sa
rizobakterijama koje podstiču rast biljaka (PGPR) povećava brojnost diazotrofa u
rizosferi, morfološke i produktivne osobine i prinos kukuruza uz očuvanje i
unapređenje agroekosistema. Shodno tome, inokulacija semena može predstavljati
važnu strategiju za poboljšanje komercijalne proizvodnje kukuruza i smanjuje
uticaj procesa proizvodnje kukuruza na životnu sredinu.
C3  - Proceedings of the 13th International Symposium „Modern Trends in Livestock Production“
T1  - Inoculation of maize with PGPR.
T1  - Inokulacija kukuruza sa PGPR
SP  - 322
EP  - 344
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_768
ER  - 

@conference{
author = "Mandić, Violeta and Đorđević, Snežana and Bijelić, Zorica and Krnjaja, Vesna and Simić, Alesksandar and Gogić, Marija and Petričević, Maja",
year = "2021-10",
abstract = "Maize is а highly productive crop intended for human and
animal nutrition, and industrial processing. The standard agrotechnical measures
are used in its production. However, the utilization of bacterial inoculants is
becoming more popular due to their effect on increased soil quality, plant
performance, and yield while preserving and enhancing the agroecosystem. In
addition, bacterial inoculants can be applied together with pesticides used in seed
treatments. Our earlier results showed that the inoculation of maize seed with
plant-growth-promoting rhizobacteria (PGPR) increased the number of diazotrophs
bacteria in the rhizosphere, morphological and productive traits, and yield of
maize. Accordingly, seed inoculation may represent an important strategy to
improve commercial maize production and reduce the environmental impact of
maize production processes., Kukuruz je visokoproduktivni usev namenjen za ishranu ljudi i životinja i
industrijsku preradu. U njegovoj proizvodnji koriste se standardne agrotehničke
mere. Međutim, upotreba bakterijskih inokulanata postaje sve popularnija zbog
povećanja kvaliteta zemljišta, performansi biljaka i prinosa. Pored toga, bakterijski
inokulanti se mogu primeniti zajedno sa pesticidima koji se koriste u tretiranju
semena. Naši raniji rezultati pokazali su da inokulacija semena kukuruza sa
rizobakterijama koje podstiču rast biljaka (PGPR) povećava brojnost diazotrofa u
rizosferi, morfološke i produktivne osobine i prinos kukuruza uz očuvanje i
unapređenje agroekosistema. Shodno tome, inokulacija semena može predstavljati
važnu strategiju za poboljšanje komercijalne proizvodnje kukuruza i smanjuje
uticaj procesa proizvodnje kukuruza na životnu sredinu.",
journal = "Proceedings of the 13th International Symposium „Modern Trends in Livestock Production“",
title = "Inoculation of maize with PGPR., Inokulacija kukuruza sa PGPR",
pages = "322-344",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_768"
}

Mandić, V., Đorđević, S., Bijelić, Z., Krnjaja, V., Simić, A., Gogić, M.,& Petričević, M.. (2021-10). Inoculation of maize with PGPR.. in Proceedings of the 13th International Symposium „Modern Trends in Livestock Production“, 322-344.
https://hdl.handle.net/21.15107/rcub_ristocar_768

Mandić V, Đorđević S, Bijelić Z, Krnjaja V, Simić A, Gogić M, Petričević M. Inoculation of maize with PGPR.. in Proceedings of the 13th International Symposium „Modern Trends in Livestock Production“. 2021;:322-344.
https://hdl.handle.net/21.15107/rcub_ristocar_768 .

Mandić, Violeta, Đorđević, Snežana, Bijelić, Zorica, Krnjaja, Vesna, Simić, Alesksandar, Gogić, Marija, Petričević, Maja, "Inoculation of maize with PGPR." in Proceedings of the 13th International Symposium „Modern Trends in Livestock Production“ (2021-10):322-344,
https://hdl.handle.net/21.15107/rcub_ristocar_768 .

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021-07
UR  - http://r.istocar.bg.ac.rs/handle/123456789/743
AB  - The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (Genealogical Concordance Phylogenetic Species Recognition) revealed the existence of 15 phylogenetic species within what is now recognised as the Fusarium graminearum Species Complex (FGSC) (Sarver et al. 2011). During 1996-2008, a MRIZP collection of FGSC isolates was established and isolates originating from wheat (5), maize (3) and barely (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5-53.1 × 4.6-5.4 µm, No 50) of all 10 isolates on PDA were consistent with descriptions of F. graminearum (O'Donnell et al. 2004, Leslie and Summerell 2006). Total DNA was isolated from mycelium removed from 7-day old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3 and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in NCBI under accession numbers MF974399 - MF974408 (TEF-1α), MG063783 - MG063792 (β-tubulin) and MF999139 - MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006), shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728) and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99% to 100% nucleotide similarity with F. graminearum NRRL 28439 (O'Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterhazy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day colonies on PDA, and 30 ml of a conidia suspension (1x105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1-7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4 -5.1 of F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Toth et al. 2005) and Korea on barley, corn and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness and toxicological profile of F. vorosii.
PB  - The American Phytopathological Society, USA
T2  - Plant Disease
T1  - First report of head blight of wheat caused by Fusarium vorosii in Serbia
DO  - 10.1094/PDIS-04-21-0715-PDN
ER  - 

@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2021-07",
abstract = "The cosmopolitan species Fusarium graminearum Schwabe directly reduces yield, as well as grain quality of cereals, due to its ability to synthesize mycotoxins. Previously it was considered to be one species occurring on all continents. However, phylogenetic analysis employing the GCPSR method (Genealogical Concordance Phylogenetic Species Recognition) revealed the existence of 15 phylogenetic species within what is now recognised as the Fusarium graminearum Species Complex (FGSC) (Sarver et al. 2011). During 1996-2008, a MRIZP collection of FGSC isolates was established and isolates originating from wheat (5), maize (3) and barely (2) were selected for further study. Morphological features including the appearance of colonies and macroconidia (average size 38.5-53.1 × 4.6-5.4 µm, No 50) of all 10 isolates on PDA were consistent with descriptions of F. graminearum (O'Donnell et al. 2004, Leslie and Summerell 2006). Total DNA was isolated from mycelium removed from 7-day old colonies of single-spore isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden). Further identification was based on amplification and sequencing of elongation factor TEF-1α, histone H3 and β-tubulin in both directions, with primers ef1/ef2, H3-1a/H3-1b and T1/T22, respectively (Jacobs et al. 2010). The sequences were deposited in NCBI under accession numbers MF974399 - MF974408 (TEF-1α), MG063783 - MG063792 (β-tubulin) and MF999139 - MF999148 (histone H3). Sequence analysis was performed using BLAST while genetic similarity was calculated using MEGA 6.0 software. Isolate 1339 originating from wheat (collected at the locality of Kikinda in 2006), shared 100% nucleotide identity with TEF-1α (DQ459745), histone H3 (DQ459728) and β-tubulin (DQ459643) of F. vorosii isolate NRRL37605 (Starkey et al. 2007). The remaining nine isolates were identified as F. graminearum as they shared 99% to 100% nucleotide similarity with F. graminearum NRRL 28439 (O'Donnell et al. 2004). Pathogenicity was tested using artificial inoculations of spikes during wheat flowering (Mesterhazy et al. 1999). Thirty classes were inoculated with each isolate, in three replicates. Inoculum was prepared from 7-day colonies on PDA, and 30 ml of a conidia suspension (1x105 conidia/ml) was used. Control plants were inoculated with sterile water. Three weeks after inoculation, typical Fusarium head blight symptoms were visible on inoculated plants, from which all 10 isolates were successfully reisolated. Control spikes remained symptomless. Disease severity was estimated on the 1-7 scale (Blandino et al. 2012). Average pathogenicity of the F. vorosii isolate 1339 was 1.9, and 2.4 -5.1 of F. graminearum isolates. Toxin production was determined using gas chromatography-tandem mass spectrometry. Kernels inoculated with the 10 isolates were ground and tested for the presence of deoxynivalenol (DON) and its acetyl derivatives 3ADON, 15ADON and NIV. F. vorosii isolate 1339 possessed the 15ADON chemotype, as well as eight F. graminearum isolates, while only one F. graminearum isolate was 3ADON chemotype. To date, F. vorosii has only been detected in Hungary on wheat (Toth et al. 2005) and Korea on barley, corn and rice (Lee et al. 2016). This is the first report of F. vorosii in Serbia, which is of great importance, because it indicates the spread of this toxigenic species. Further studies should be focused on determining the distribution, aggressiveness and toxicological profile of F. vorosii.",
publisher = "The American Phytopathological Society, USA",
journal = "Plant Disease",
title = "First report of head blight of wheat caused by Fusarium vorosii in Serbia",
doi = "10.1094/PDIS-04-21-0715-PDN"
}

Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2021-07). First report of head blight of wheat caused by Fusarium vorosii in Serbia. in Plant Disease
The American Phytopathological Society, USA..
https://doi.org/10.1094/PDIS-04-21-0715-PDN

Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First report of head blight of wheat caused by Fusarium vorosii in Serbia. in Plant Disease. 2021;.
doi:10.1094/PDIS-04-21-0715-PDN .

Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of head blight of wheat caused by Fusarium vorosii in Serbia" in Plant Disease (2021-07),
https://doi.org/10.1094/PDIS-04-21-0715-PDN . .

TY  - JOUR
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Krnjaja, Vesna
AU  - Živković, Sanja
AU  - Trkulja, Vojislav
PY  - 2021-05
UR  - http://r.istocar.bg.ac.rs/handle/123456789/742
AB  - In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.
PB  - Verlag Eugen Ulmer GmbH, Germany
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia
DO  - 10.1007/s41348-021-00476-5
ER  - 

@article{
author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Živković, Sanja and Trkulja, Vojislav",
year = "2021-05",
abstract = "In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.",
publisher = "Verlag Eugen Ulmer GmbH, Germany",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia",
doi = "10.1007/s41348-021-00476-5"
}

Vasić, T., Jevremović, D., Krnjaja, V., Živković, S.,& Trkulja, V.. (2021-05). Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection
Verlag Eugen Ulmer GmbH, Germany..
https://doi.org/10.1007/s41348-021-00476-5

Vasić T, Jevremović D, Krnjaja V, Živković S, Trkulja V. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection. 2021;.
doi:10.1007/s41348-021-00476-5 .

Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Živković, Sanja, Trkulja, Vojislav, "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia" in Journal of Plant Diseases and Protection (2021-05),
https://doi.org/10.1007/s41348-021-00476-5 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Radović, Čedomir
PY  - 2021-02-25
UR  - http://r.istocar.bg.ac.rs/handle/123456789/730
AB  - The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.
T2  - Crop Protection
T1  - Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels
VL  - 144
SP  - 105601
DO  - https://doi.org/10.1016/j.cropro.2021.105601
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja and Radović, Čedomir",
year = "2021-02-25",
abstract = "The aim of this investigation was to estimate the influence of four nitrogen (N) rates (0, 60, 120 and 180 kg ha−1) and two ear inoculation treatments (inoculated with F. verticillioides and control) of two Serbian maize hybrids NS 6030 and ZP 666 (FAO 600 maturity groups) on some productive and quality parameters. The experiments were conducted in the two growing seasons (2013–2014). 

Nitrogen rates demonstrated a highly significant (P ≤ 0.01) influence on the number of kernels per ear (NKE), thousand kernel weight (TKW), the incidence of Fusarium spp. (F. proliferatum, F. subglutinans, and F. verticillioides) and level of total fumonisins B1, B2, and B3 (FBs). The ear treatment showed a highly significant (P ≤ 0.01) effect on maize quality parameters (NIK, the incidence of Fusarium spp. and FBs level) and significant (P ≤ 0.05) for TKW. 

Among identified Fusarium spp., F. verticillioides was predominant, with the highest presence found at 120 (62.41%) and 180 kg N ha−1 (61.81%). The occurrence of F. proliferatum was not significant at 0, 60, and 120 kg N ha−1, with the highest value recorded at 120 kg N ha− 1 (0.69%). The highest incidence of F. subglutinans was at 60 kg N ha−1 (4.94%). The higher presence of F. verticillioides was obtained in the inoculated (84.55%) compared to control ear treatments (36.59%), while it was vice versa for the incidence of F. proliferatum and F. subglutinans. The level of FBs was the highest at 120 kg N ha−1 (21.72 mg kg−1), especially in inoculated ear treatments (32.84 mg kg−1). A positive and significant relationship was found for NIK, with F. verticillioides and FBs level.

Investigated years significantly influenced the incidence of Fusarium spp. In addition, there was a significant effect of hybrids on NIK, the incidence of Fusarium spp., and FBs levels. A higher incidence of F. proliferatum and F. verticillioides and increased FBs level were recorded in NS 6030 hybrid compare to ZP 666. The mean FBs level in all the treatments was above a maximum limit (4 mg kg−1) adopted by EU regulation for unprocessed maize. The N rates did not have a consistent effect on the incidence of Fusarium spp. and FBs level. However, a hybrid effect was more consistent on the maize quality parameters, whereby the hybrid NS 6030 was more sensitive to fungal fumonisin producers and FBs contamination than ZP 666. In addition, the effect of the years significantly influenced the incidence of Fusarium spp., but did not affect on FBs level. 

These results indicate the importance of studying relationships between different factors affecting the occurrence of fungal contamination, therefore, improving preventative measures of maize protection would reduce the risks on animal and human health.",
journal = "Crop Protection",
title = "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels",
volume = "144",
pages = "105601",
doi = "https://doi.org/10.1016/j.cropro.2021.105601"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T., Vasić, T.,& Radović, Č.. (2021-02-25). Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection, 144, 105601.
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Vasić T, Radović Č. Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels. in Crop Protection. 2021;144:105601.
doi:https://doi.org/10.1016/j.cropro.2021.105601 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Vasić, Tanja, Radović, Čedomir, "Influence of nitrogen rates and Fusarium verticillioides infection on Fusarium spp. and fumonisin contamination of maize kernels" in Crop Protection, 144 (2021-02-25):105601,
https://doi.org/https://doi.org/10.1016/j.cropro.2021.105601 . .

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Nikolić, Milica
AU  - Vasić, Tanja
AU  - Delić, Nikola
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/738
AB  - The aim of this study was to evaluate fungal infection, with a 
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a 
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian 
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest 
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of 
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F. 
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in 
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in 
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of 
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13) 
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars 
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A 
statistically significant difference in DON level was found between the two tested 
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to 
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant 
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in 
the tested seed of Italian ryegrass indicate on potentially significant participation of 
DON in FHB pathogenesis, as well as a potential risk for the quality seed 
production, feed safety and the food chain in general. In Serbia, this is the first 
report about Fusarium infection and DON presence in ryegrass seed.
C3  - Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631
T1  - Fusarium spp. and deoxynivalenol contamination of ryegrass seeds
SP  - 622
EP  - 631
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_738
ER  - 

@conference{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Nikolić, Milica and Vasić, Tanja and Delić, Nikola",
year = "2021",
abstract = "The aim of this study was to evaluate fungal infection, with a 
special focus on Fusarium spp. and deoxynivalenol (DON) presence, as a 
participant in Fusarium head blight (FHB) pathogenesis in two cultivars of Italian 
ryegrass K-13 and K-29. A total of 24 seed samples were collected during harvest 
in 2019.
By mycological analyses, Acremonium spp., Alternaria spp., Fusarium
spp. and non-sporulating species (Mycelia sterilia) were isolated on the seeds of 
both cultivars of Italian ryegrass. Among Fusarium spp., four species, F. 
graminearum, F. poae, F. proliferatum and F. subglutinans were identified in 
cultivar K-13 and three species, F. graminearum, F. poae and F. subglutinans, in 
cultivar K-29. F. graminearum and F. poae were identified as FHB pathogens, of 
which F. graminearum was dominant in both cultivars with 20.5% (cultivar K-13) 
and 32% (cultivar K-29) compared to F. poae which was present in both cultivars 
with a frequency of 0.5%. The frequency of DON positive samples was 100%. A 
statistically significant difference in DON level was found between the two tested 
cultivars, with a higher DON level in cultivar K-29 (5334.33 μg kg–1
) compared to 
cultivar K-13 (4738.58 μg kg–1
).
The obtained results indicate that two Fusarium species, F. graminearum
and F. poae, were FHB pathogens, with F. graminearum as the predominant 
species in both cultivars of Italian ryegrass. High DON levels (>3000 μg kg–1
) in 
the tested seed of Italian ryegrass indicate on potentially significant participation of 
DON in FHB pathogenesis, as well as a potential risk for the quality seed 
production, feed safety and the food chain in general. In Serbia, this is the first 
report about Fusarium infection and DON presence in ryegrass seed.",
journal = "Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631",
title = "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds",
pages = "622-631",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_738"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Nikolić, M., Vasić, T.,& Delić, N.. (2021). Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631, 622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738

Krnjaja V, Mandić V, Bijelić Z, Stanković S, Nikolić M, Vasić T, Delić N. Fusarium spp. and deoxynivalenol contamination of ryegrass seeds. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631. 2021;:622-631.
https://hdl.handle.net/21.15107/rcub_ristocar_738 .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Stanković, Slavica, Nikolić, Milica, Vasić, Tanja, Delić, Nikola, "Fusarium spp. and deoxynivalenol contamination of ryegrass seeds" in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 622-631 (2021):622-631,
https://hdl.handle.net/21.15107/rcub_ristocar_738 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Petrović, Tanja
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Radović, Čedomir
AU  - Delić, Nikola
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/732
AB  - The aim of this study was to establish the total fungal (mould)
count in 79 commercial pig farm feed samples (42 samples for piglets, 29 samples 
for fattening pigs and 8 samples for sows) collected from the Serbian feed 
producers during the three-year period (2017–2019), as well as to evaluate the
percentage of contamination samples by fungi, especially species from Aspergillus,
Fusarium and Penicillium genera. Using standard mycological methods, the total 
count and identification of fungi were determined. Total fungal count was ranging 
from 1 × 102 to 1.41 × 105 cfu g–1 in the feed samples for piglets. Furthermore, in 
the feed samplesfor fattening pigs it ranged from 1 × 102 to 2.54 × 105 cfu g–1
, and 
from 1 × 102 to 1.93 × 105 cfu g–1 in the feed samples for sows. It has established 
the impermissible limit of total fungal count in 3.45% and 4.76% of a feed for 
fattening pigs and feed for piglets, respectively. Statistical analysis of the total 
number of fungi did not establish significant differences between the examined 
feed groups. Fusarium species were present in the most feed samples for fattening 
pigs (65.52%), followed by the feed for sows (62.50%) and piglets (47.62%). 
Aspergillus species were determined in 59.52, 58.62 and 37.50% feed samples for 
piglets, fattening pigs and sows, respectively. Penicillium species contaminated the 
lowest percentage of feed samples for fattening pigs (27.59%) and the highest 
percentage of feed samples for sows (37.50%). In a small number of samples 
Alternaria, Mucor, and Rhizopus species were identified. Based on these results, it 
can be concluded that the investigation of fungal contamination is an important 
indicator of a hygienic condition of feed intended for the nutrition of farm pigs. In 
addition, the percentage of fungal contamination of the examined samples indicates 
a potentially high risk to animal health. Due to that, a regular mycological 
evaluation is necessary to assess nutritional quality as one of the fundamental 
criteria for feed safety.
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of pig farm feeds
VL  - 37
IS  - 2
SP  - 139
EP  - 147
DO  - 10.2298/BAH2102139K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Radović, Čedomir and Delić, Nikola",
year = "2021",
abstract = "The aim of this study was to establish the total fungal (mould)
count in 79 commercial pig farm feed samples (42 samples for piglets, 29 samples 
for fattening pigs and 8 samples for sows) collected from the Serbian feed 
producers during the three-year period (2017–2019), as well as to evaluate the
percentage of contamination samples by fungi, especially species from Aspergillus,
Fusarium and Penicillium genera. Using standard mycological methods, the total 
count and identification of fungi were determined. Total fungal count was ranging 
from 1 × 102 to 1.41 × 105 cfu g–1 in the feed samples for piglets. Furthermore, in 
the feed samplesfor fattening pigs it ranged from 1 × 102 to 2.54 × 105 cfu g–1
, and 
from 1 × 102 to 1.93 × 105 cfu g–1 in the feed samples for sows. It has established 
the impermissible limit of total fungal count in 3.45% and 4.76% of a feed for 
fattening pigs and feed for piglets, respectively. Statistical analysis of the total 
number of fungi did not establish significant differences between the examined 
feed groups. Fusarium species were present in the most feed samples for fattening 
pigs (65.52%), followed by the feed for sows (62.50%) and piglets (47.62%). 
Aspergillus species were determined in 59.52, 58.62 and 37.50% feed samples for 
piglets, fattening pigs and sows, respectively. Penicillium species contaminated the 
lowest percentage of feed samples for fattening pigs (27.59%) and the highest 
percentage of feed samples for sows (37.50%). In a small number of samples 
Alternaria, Mucor, and Rhizopus species were identified. Based on these results, it 
can be concluded that the investigation of fungal contamination is an important 
indicator of a hygienic condition of feed intended for the nutrition of farm pigs. In 
addition, the percentage of fungal contamination of the examined samples indicates 
a potentially high risk to animal health. Due to that, a regular mycological 
evaluation is necessary to assess nutritional quality as one of the fundamental 
criteria for feed safety.",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of pig farm feeds",
volume = "37",
number = "2",
pages = "139-147",
doi = "10.2298/BAH2102139K"
}

Krnjaja, V., Stanojković, A., Petrović, T., Mandić, V., Bijelić, Z., Radović, Č.,& Delić, N.. (2021). Fungal contamination of pig farm feeds. in Biotechnology in Animal Husbandry, 37(2), 139-147.
https://doi.org/10.2298/BAH2102139K

Krnjaja V, Stanojković A, Petrović T, Mandić V, Bijelić Z, Radović Č, Delić N. Fungal contamination of pig farm feeds. in Biotechnology in Animal Husbandry. 2021;37(2):139-147.
doi:10.2298/BAH2102139K .

Krnjaja, Vesna, Stanojković, Aleksandar, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Radović, Čedomir, Delić, Nikola, "Fungal contamination of pig farm feeds" in Biotechnology in Animal Husbandry, 37, no. 2 (2021):139-147,
https://doi.org/10.2298/BAH2102139K . .

TY  - CONF
AU  - Dragičević, Vesna
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Šenk, Milena
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Kresović, Branka
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/739
AB  - Malnutrition is a global problem, hitting both, people and 
animals. Due to the many factors, such as climate change, soil depletion, 
anthropogenic impact, including irresponsible soil management, high fertilization 
rates with macro-nutrients, crops lack in essential nutrients, particularly minerals, 
Mg, Fe, Zn, etc., as well as important vitamins, like carotenoids, vitamins from B 
group. Also, some arable soils have naturally low fertility. All of that negatively 
affect production of domestic animal, including animal health and quality of 
livestock products. To mitigate malnutrition, a bio-fortification strategy was 
developed. It is based on increase in the concentration of essential nutrients in food 
and feed, and also to promote further bio-availability from digestive organs. Bio fortification implies various practices. Two main types of bio-fortification were 
developed: genetic bio-fortification, and agronomic bio-fortification. The first one 
uses standard breeding techniques, marker assistant selection, transgenic 
approaches, genome editing, etc., to develop highly-efficient genotypes, that are 
able to absorb and accumulate higher concentrations of essential nutrients in 
biomass and grains. Agronomic bio-fortification uses different practices, such is 
special fertilizers, enriched with essential minerals, foliar fertilizers, bio-fertilizers, 
growth hormones and enhancers, and some lesser known sustainable practices, like 
inter-cropping, cover cropping, in order to increase crop yields, as well as the 
concentration of essential nutrients. The bio-fortification approach, based on the 
development and commercialisation of highly efficient genotypes, as well as 
agricultural practices that enable and support better absorption and accumulation of 
essential nutrients option that is safer for both, people and domestic animals. 
Mutual increase in concentration of essential minerals, vitamins, and other 
promoting substances is of a particular importance for bio-fortification programs, 
increasing efficiency and success of applied practices, thus positively reflecting on 
animal health and wellbeing.
C3  - Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 287-307
T1  - Biofortification as a way of nutrient dense feed production
SP  - 287
EP  - 307
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_739
ER  - 

@conference{
author = "Dragičević, Vesna and Simić, Milena and Brankov, Milan and Šenk, Milena and Krnjaja, Vesna and Mandić, Violeta and Kresović, Branka",
year = "2021",
abstract = "Malnutrition is a global problem, hitting both, people and 
animals. Due to the many factors, such as climate change, soil depletion, 
anthropogenic impact, including irresponsible soil management, high fertilization 
rates with macro-nutrients, crops lack in essential nutrients, particularly minerals, 
Mg, Fe, Zn, etc., as well as important vitamins, like carotenoids, vitamins from B 
group. Also, some arable soils have naturally low fertility. All of that negatively 
affect production of domestic animal, including animal health and quality of 
livestock products. To mitigate malnutrition, a bio-fortification strategy was 
developed. It is based on increase in the concentration of essential nutrients in food 
and feed, and also to promote further bio-availability from digestive organs. Bio fortification implies various practices. Two main types of bio-fortification were 
developed: genetic bio-fortification, and agronomic bio-fortification. The first one 
uses standard breeding techniques, marker assistant selection, transgenic 
approaches, genome editing, etc., to develop highly-efficient genotypes, that are 
able to absorb and accumulate higher concentrations of essential nutrients in 
biomass and grains. Agronomic bio-fortification uses different practices, such is 
special fertilizers, enriched with essential minerals, foliar fertilizers, bio-fertilizers, 
growth hormones and enhancers, and some lesser known sustainable practices, like 
inter-cropping, cover cropping, in order to increase crop yields, as well as the 
concentration of essential nutrients. The bio-fortification approach, based on the 
development and commercialisation of highly efficient genotypes, as well as 
agricultural practices that enable and support better absorption and accumulation of 
essential nutrients option that is safer for both, people and domestic animals. 
Mutual increase in concentration of essential minerals, vitamins, and other 
promoting substances is of a particular importance for bio-fortification programs, 
increasing efficiency and success of applied practices, thus positively reflecting on 
animal health and wellbeing.",
journal = "Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 287-307",
title = "Biofortification as a way of nutrient dense feed production",
pages = "287-307",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_739"
}

Dragičević, V., Simić, M., Brankov, M., Šenk, M., Krnjaja, V., Mandić, V.,& Kresović, B.. (2021). Biofortification as a way of nutrient dense feed production. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 287-307, 287-307.
https://hdl.handle.net/21.15107/rcub_ristocar_739

Dragičević V, Simić M, Brankov M, Šenk M, Krnjaja V, Mandić V, Kresović B. Biofortification as a way of nutrient dense feed production. in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 287-307. 2021;:287-307.
https://hdl.handle.net/21.15107/rcub_ristocar_739 .

Dragičević, Vesna, Simić, Milena, Brankov, Milan, Šenk, Milena, Krnjaja, Vesna, Mandić, Violeta, Kresović, Branka, "Biofortification as a way of nutrient dense feed production" in Proceedings of the 13th International Symposium "Modern Trends in Livestock Production", 6 - 8 October 2021, Belgrade, Serbia, 287-307 (2021):287-307,
https://hdl.handle.net/21.15107/rcub_ristocar_739 .

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Đorđević, Snežana
AU  - Brankov, Milan
AU  - Mićić, Nenad
AU  - Stanojković, Aleksandar
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/729
AB  - Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage
maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the
effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and
qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial
(two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem
diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably
due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent
fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage
increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay
of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain
filling.
T2  - The Journal of Animal and Plant Sciences
T1  - Harvest time effect on quantitative and qualitative parameters of forage maize
VL  - 31
IS  - 1
DO  - 10.36899/JAPS.2021.1.0198
ER  - 

@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Đorđević, Snežana and Brankov, Milan and Mićić, Nenad and Stanojković, Aleksandar",
year = "2021",
abstract = "Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage
maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the
effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and
qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial
(two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem
diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably
due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent
fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage
increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay
of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain
filling.",
journal = "The Journal of Animal and Plant Sciences",
title = "Harvest time effect on quantitative and qualitative parameters of forage maize",
volume = "31",
number = "1",
doi = "10.36899/JAPS.2021.1.0198"
}

Mandić, V., Bijelić, Z., Krnjaja, V., Đorđević, S., Brankov, M., Mićić, N.,& Stanojković, A.. (2021). Harvest time effect on quantitative and qualitative parameters of forage maize. in The Journal of Animal and Plant Sciences, 31(1).
https://doi.org/10.36899/JAPS.2021.1.0198

Mandić V, Bijelić Z, Krnjaja V, Đorđević S, Brankov M, Mićić N, Stanojković A. Harvest time effect on quantitative and qualitative parameters of forage maize. in The Journal of Animal and Plant Sciences. 2021;31(1).
doi:10.36899/JAPS.2021.1.0198 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Đorđević, Snežana, Brankov, Milan, Mićić, Nenad, Stanojković, Aleksandar, "Harvest time effect on quantitative and qualitative parameters of forage maize" in The Journal of Animal and Plant Sciences, 31, no. 1 (2021),
https://doi.org/10.36899/JAPS.2021.1.0198 . .

TY  - CONF
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Petričević, Maja
AU  - Stanojković, Aleksandar
AU  - Maksimović, Nevena
AU  - Caro Petrović, Violeta
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/706
AB  - Given that Serbia (without Kosovo) has large areas under grassland, the aim of this study was
to analyze the variation and trend of the areas, total production and yields over 71 years
(1948-2018) and prognosis of yield in the next 30 years. The results indicated that the area of
grasslands in Serbia declined over the last decade. Most grasslands in hilly-mountain region
were degraded and without application of agrotechnical - remedial measures so that large
areas were lost. In Autonomous Province of Vojvodina, areas of grasslands have been
declining because have been converted into arable land. Total production of meadows
increased while total production of pastures decreased during the observed period. The
grasslands areas in Serbia showed low yields. In the Vojvodina region, meadows showed
decrease in forage productivity, while pastures showed increase in forage productivity. Areas
under meadows and pastures should be recultivated and brought into production in
accordance with the needs of livestock development. This will result in higher quantity and
higher quality production of green mass and hay. Unfortunately, depopulation and underrepresentation
of animals per unit area represented a limiting factor for grassland exploitation.
On the other hand, in the future yield growth rates of grasslands in Serbia and Central Serbia
and pastures in Vojvodina will very low increase. Contrary, yields growth rates of meadows
in Vojvodina will decrease by 2050.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB), Italy
PB  - International Society of Environment and Rural Development, Japan
PB  - Balkan Environmental Association (B.EN.A), Greece
PB  - Centre for Development Research, University of Natural Resources and Life Sciences (BOKU), Austria
PB  - Perm State Agro-Technological University, Russia
PB  - Voronezh State Agricultural University named after Peter The Great, Russia
PB  - Faculty of Bioeconomy Development, Vytautas Magnus University, Lithuania
PB  - Enterprise Europe Network (EEN) Faculty of Agriculture, University of Akdeniz - Antalya, Turkey
PB  - Selçuk University, Turkey
PB  - University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania
PB  - Slovak University of Agriculture in Nitra, Slovakia
PB  - Ukrainian Institute for Plant Variety Examination, Kyiv, Ukraine
PB  - National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
PB  - Valahia University of Targoviste, Romania
PB  - National Scientific Center „Institute of Agriculture of NAAS“, Kyiv, Ukraine
PB  - Saint Petersburg State Forest Technical University, Russia
PB  - University of Valencia, Spain Faculty of Agriculture, Cairo University, Egypt
PB  - Tarbiat Modares University, Iran
PB  - Chapingo Autonomous University, Mexico
PB  - Department of Agricultural, Food and Environmental Sciences, University of Perugia, Italy
PB  - Higher Institute of Agronomy, Chott Mariem-Sousse, Tunisia
PB  - Watershed Management Society of Iran Institute of Animal Science - Kostinbrod, Bulgaria
PB  - Faculty of Agriculture, University of Banja Luka, Bosnia and Herzegovina
PB  - Faculty of Economics Brcko, University of East Sarajevo, Bosnia and Herzegovina
PB  - Biotechnical Faculty, University of Montenegro, Montenegro
PB  - Institute of Field and Vegetable Crops, Serbia
PB  - Institute of Lowland Forestry and Environment, Serbia
PB  - Institute for Science Application in Agriculture, Serbia
PB  - Agricultural Institute of Republic of Srpska - Banja Luka, Bosnia and Herzegovina
PB  - Maize Research Institute “Zemun Polje”, Serbia
PB  - Faculty of Agriculture, University of Novi Sad, Serbia
PB  - Institute for Animal Science, Ss. Cyril and Methodius University in Skopje, Macedonia
C3  - Book of proceedings XI International Scientific Agriculture Symposium “Agrosym 2020”
T1  - The state and production of grasslands in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_706
ER  - 

@conference{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Petričević, Maja and Stanojković, Aleksandar and Maksimović, Nevena and Caro Petrović, Violeta",
year = "2020",
abstract = "Given that Serbia (without Kosovo) has large areas under grassland, the aim of this study was
to analyze the variation and trend of the areas, total production and yields over 71 years
(1948-2018) and prognosis of yield in the next 30 years. The results indicated that the area of
grasslands in Serbia declined over the last decade. Most grasslands in hilly-mountain region
were degraded and without application of agrotechnical - remedial measures so that large
areas were lost. In Autonomous Province of Vojvodina, areas of grasslands have been
declining because have been converted into arable land. Total production of meadows
increased while total production of pastures decreased during the observed period. The
grasslands areas in Serbia showed low yields. In the Vojvodina region, meadows showed
decrease in forage productivity, while pastures showed increase in forage productivity. Areas
under meadows and pastures should be recultivated and brought into production in
accordance with the needs of livestock development. This will result in higher quantity and
higher quality production of green mass and hay. Unfortunately, depopulation and underrepresentation
of animals per unit area represented a limiting factor for grassland exploitation.
On the other hand, in the future yield growth rates of grasslands in Serbia and Central Serbia
and pastures in Vojvodina will very low increase. Contrary, yields growth rates of meadows
in Vojvodina will decrease by 2050.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB), Italy, International Society of Environment and Rural Development, Japan, Balkan Environmental Association (B.EN.A), Greece, Centre for Development Research, University of Natural Resources and Life Sciences (BOKU), Austria, Perm State Agro-Technological University, Russia, Voronezh State Agricultural University named after Peter The Great, Russia, Faculty of Bioeconomy Development, Vytautas Magnus University, Lithuania, Enterprise Europe Network (EEN) Faculty of Agriculture, University of Akdeniz - Antalya, Turkey, Selçuk University, Turkey, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania, Slovak University of Agriculture in Nitra, Slovakia, Ukrainian Institute for Plant Variety Examination, Kyiv, Ukraine, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine, Valahia University of Targoviste, Romania, National Scientific Center „Institute of Agriculture of NAAS“, Kyiv, Ukraine, Saint Petersburg State Forest Technical University, Russia, University of Valencia, Spain Faculty of Agriculture, Cairo University, Egypt, Tarbiat Modares University, Iran, Chapingo Autonomous University, Mexico, Department of Agricultural, Food and Environmental Sciences, University of Perugia, Italy, Higher Institute of Agronomy, Chott Mariem-Sousse, Tunisia, Watershed Management Society of Iran Institute of Animal Science - Kostinbrod, Bulgaria, Faculty of Agriculture, University of Banja Luka, Bosnia and Herzegovina, Faculty of Economics Brcko, University of East Sarajevo, Bosnia and Herzegovina, Biotechnical Faculty, University of Montenegro, Montenegro, Institute of Field and Vegetable Crops, Serbia, Institute of Lowland Forestry and Environment, Serbia, Institute for Science Application in Agriculture, Serbia, Agricultural Institute of Republic of Srpska - Banja Luka, Bosnia and Herzegovina, Maize Research Institute “Zemun Polje”, Serbia, Faculty of Agriculture, University of Novi Sad, Serbia, Institute for Animal Science, Ss. Cyril and Methodius University in Skopje, Macedonia",
journal = "Book of proceedings XI International Scientific Agriculture Symposium “Agrosym 2020”",
title = "The state and production of grasslands in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_706"
}

Mandić, V., Bijelić, Z., Krnjaja, V., Petričević, M., Stanojković, A., Maksimović, N.,& Caro Petrović, V.. (2020). The state and production of grasslands in Serbia. in Book of proceedings XI International Scientific Agriculture Symposium “Agrosym 2020”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_ristocar_706

Mandić V, Bijelić Z, Krnjaja V, Petričević M, Stanojković A, Maksimović N, Caro Petrović V. The state and production of grasslands in Serbia. in Book of proceedings XI International Scientific Agriculture Symposium “Agrosym 2020”. 2020;.
https://hdl.handle.net/21.15107/rcub_ristocar_706 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Petričević, Maja, Stanojković, Aleksandar, Maksimović, Nevena, Caro Petrović, Violeta, "The state and production of grasslands in Serbia" in Book of proceedings XI International Scientific Agriculture Symposium “Agrosym 2020” (2020),
https://hdl.handle.net/21.15107/rcub_ristocar_706 .

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Đorđević, Nikola
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Petričević, Maja
AU  - Brankov, Milan
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/704
AB  - The successful production of soybeans is largely dependent on the sowing time, because
every sowing outside the optimal time contributes significantly to yield losses. This field study
aimed to evaluate the e ects of sowing time (optimal—April 5; late—April 27) on the quantitative
and quality traits of three soybean genotypes (Galina—0 maturity group; Sava—I maturity group;
and Rubin—II maturity group) under dryland conditions in Vojvodina Province (Serbia) during
2017 and 2018. The genotype Sava had higher yield in climatic-unfavorable 2017, while Rubin had a
higher yield in climatic-favorable 2018. The yields significantly decreased when the soybeans were
sown in late April due to reductions in the number of pods per plant, seed weight per plant, and
1000-seed weight. The reduction in yield components was likely due to the accelerated senescence of
plants and the negative e ect of high temperature and low precipitation during the seed filling stage.
Accordingly, the various sowing times and properly chosen genotypes provide a better utilization of
soil and water resources. A proper genotype selection and sowing time can contribute to a high yield.
At the same time, the protein and oil contents can be altered by the sowing time, especially under
water stress during the reproductive stage.
PB  - MDPI
T2  - Agriculture
T1  - Genotype and sowing time effects on soybean yield and quality
VL  - 10
IS  - 11
SP  - 502
DO  - 10.3390/agriculture10110502
ER  - 

@article{
author = "Mandić, Violeta and Đorđević, Snežana and Đorđević, Nikola and Bijelić, Zorica and Krnjaja, Vesna and Petričević, Maja and Brankov, Milan",
year = "2020",
abstract = "The successful production of soybeans is largely dependent on the sowing time, because
every sowing outside the optimal time contributes significantly to yield losses. This field study
aimed to evaluate the e ects of sowing time (optimal—April 5; late—April 27) on the quantitative
and quality traits of three soybean genotypes (Galina—0 maturity group; Sava—I maturity group;
and Rubin—II maturity group) under dryland conditions in Vojvodina Province (Serbia) during
2017 and 2018. The genotype Sava had higher yield in climatic-unfavorable 2017, while Rubin had a
higher yield in climatic-favorable 2018. The yields significantly decreased when the soybeans were
sown in late April due to reductions in the number of pods per plant, seed weight per plant, and
1000-seed weight. The reduction in yield components was likely due to the accelerated senescence of
plants and the negative e ect of high temperature and low precipitation during the seed filling stage.
Accordingly, the various sowing times and properly chosen genotypes provide a better utilization of
soil and water resources. A proper genotype selection and sowing time can contribute to a high yield.
At the same time, the protein and oil contents can be altered by the sowing time, especially under
water stress during the reproductive stage.",
publisher = "MDPI",
journal = "Agriculture",
title = "Genotype and sowing time effects on soybean yield and quality",
volume = "10",
number = "11",
pages = "502",
doi = "10.3390/agriculture10110502"
}

Mandić, V., Đorđević, S., Đorđević, N., Bijelić, Z., Krnjaja, V., Petričević, M.,& Brankov, M.. (2020). Genotype and sowing time effects on soybean yield and quality. in Agriculture
MDPI., 10(11), 502.
https://doi.org/10.3390/agriculture10110502

Mandić V, Đorđević S, Đorđević N, Bijelić Z, Krnjaja V, Petričević M, Brankov M. Genotype and sowing time effects on soybean yield and quality. in Agriculture. 2020;10(11):502.
doi:10.3390/agriculture10110502 .

Mandić, Violeta, Đorđević, Snežana, Đorđević, Nikola, Bijelić, Zorica, Krnjaja, Vesna, Petričević, Maja, Brankov, Milan, "Genotype and sowing time effects on soybean yield and quality" in Agriculture, 10, no. 11 (2020):502,
https://doi.org/10.3390/agriculture10110502 . .

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Pantelić, Vlada
AU  - Simić, Aleksandar
AU  - Dragičević, Vesna
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/683
AB  - The main nitrogen (N) sources in soybean production originate from soil fixing bacteria Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not sufficient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the effects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha−1) on quantitative and qualitative parameters and on rain use efficiency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two different growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha−1, protein content at 90 kg N ha−1 and oil content 0 kg N ha−1 (control). This study suggests that proper genotype selection and application of 60 kg N ha−1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.
PB  - MDPI
T2  - Agronomy
T1  - Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate
VL  - 10
IS  - 4
SP  - 1
EP  - 10
DO  - 10.3390/agronomy10040535
ER  - 

@article{
author = "Mandić, Violeta and Đorđević, Snežana and Bijelić, Zorica and Krnjaja, Vesna and Pantelić, Vlada and Simić, Aleksandar and Dragičević, Vesna",
year = "2020",
abstract = "The main nitrogen (N) sources in soybean production originate from soil fixing bacteria Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not sufficient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the effects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha−1) on quantitative and qualitative parameters and on rain use efficiency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two different growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha−1, protein content at 90 kg N ha−1 and oil content 0 kg N ha−1 (control). This study suggests that proper genotype selection and application of 60 kg N ha−1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.",
publisher = "MDPI",
journal = "Agronomy",
title = "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate",
volume = "10",
number = "4",
pages = "1-10",
doi = "10.3390/agronomy10040535"
}

Mandić, V., Đorđević, S., Bijelić, Z., Krnjaja, V., Pantelić, V., Simić, A.,& Dragičević, V.. (2020). Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. in Agronomy
MDPI., 10(4), 1-10.
https://doi.org/10.3390/agronomy10040535

Mandić V, Đorđević S, Bijelić Z, Krnjaja V, Pantelić V, Simić A, Dragičević V. Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. in Agronomy. 2020;10(4):1-10.
doi:10.3390/agronomy10040535 .

Mandić, Violeta, Đorđević, Snežana, Bijelić, Zorica, Krnjaja, Vesna, Pantelić, Vlada, Simić, Aleksandar, Dragičević, Vesna, "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate" in Agronomy, 10, no. 4 (2020):1-10,
https://doi.org/10.3390/agronomy10040535 . .

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Simić, Aleksandar
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Đorđević, Snežana
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/705
AB  - Field experiment was conducted to examine the impacts of two sowing dates (8 April - first date of sowing and
21 April - second date of sowing) and four nitrogen rates (0, 60, 120 and 180 kg ha-1) on the productivity of maize
hybrid ‘ZP 434’ in the Pannonian region of Serbia during 2016 and 2017 seasons. The dry period during late
vegetative development and grain filling stage in 2017 decreased ear traits, grain yield, starch and oil contents,
nitrogen agronomic (NAE) and nitrogen use efficiency (NUE). The highest number of grains per ear, starch and oil
contents, starch and oil yields and lower rainfall use efficiency (RUE) and protein content were obtained from the
early sowing date. The ear traits, grain yield, RUE, protein content, oil content and yield of starch, protein and oil
significantly increased while NAE, NUE and starch content significantly decreased with increasing nitrogen rate.
The results indicated a significant inverse correlation between starch and protein contents, which prevents the
improvement of these two parameters simultaneously. Thus, timely sowing and nitrogen input should be used as
long term management strategies for increasing maize yield and grain quality.
PB  - Istituto Sperimentale per la Cerealicoltura Sezione di Bergamo
T2  - Maydica
T1  - Sowing and fertilization strategies to improve maize productivity
VL  - 65
IS  - 12
SP  - 1
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_705
ER  - 

@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Simić, Aleksandar and Simić, Milena and Brankov, Milan and Đorđević, Snežana",
year = "2020",
abstract = "Field experiment was conducted to examine the impacts of two sowing dates (8 April - first date of sowing and
21 April - second date of sowing) and four nitrogen rates (0, 60, 120 and 180 kg ha-1) on the productivity of maize
hybrid ‘ZP 434’ in the Pannonian region of Serbia during 2016 and 2017 seasons. The dry period during late
vegetative development and grain filling stage in 2017 decreased ear traits, grain yield, starch and oil contents,
nitrogen agronomic (NAE) and nitrogen use efficiency (NUE). The highest number of grains per ear, starch and oil
contents, starch and oil yields and lower rainfall use efficiency (RUE) and protein content were obtained from the
early sowing date. The ear traits, grain yield, RUE, protein content, oil content and yield of starch, protein and oil
significantly increased while NAE, NUE and starch content significantly decreased with increasing nitrogen rate.
The results indicated a significant inverse correlation between starch and protein contents, which prevents the
improvement of these two parameters simultaneously. Thus, timely sowing and nitrogen input should be used as
long term management strategies for increasing maize yield and grain quality.",
publisher = "Istituto Sperimentale per la Cerealicoltura Sezione di Bergamo",
journal = "Maydica",
title = "Sowing and fertilization strategies to improve maize productivity",
volume = "65",
number = "12",
pages = "1-9",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_705"
}

Mandić, V., Bijelić, Z., Krnjaja, V., Simić, A., Simić, M., Brankov, M.,& Đorđević, S.. (2020). Sowing and fertilization strategies to improve maize productivity. in Maydica
Istituto Sperimentale per la Cerealicoltura Sezione di Bergamo., 65(12), 1-9.
https://hdl.handle.net/21.15107/rcub_ristocar_705

Mandić V, Bijelić Z, Krnjaja V, Simić A, Simić M, Brankov M, Đorđević S. Sowing and fertilization strategies to improve maize productivity. in Maydica. 2020;65(12):1-9.
https://hdl.handle.net/21.15107/rcub_ristocar_705 .

Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Simić, Aleksandar, Simić, Milena, Brankov, Milan, Đorđević, Snežana, "Sowing and fertilization strategies to improve maize productivity" in Maydica, 65, no. 12 (2020):1-9,
https://hdl.handle.net/21.15107/rcub_ristocar_705 .