Trkulja, Vojislav

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  • Trkulja, Vojislav (2)
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Author's Bibliography

Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia

Vasić, Tanja; Jevremović, Darko; Krnjaja, Vesna; Živković, Sanja; Trkulja, Vojislav

(Verlag Eugen Ulmer GmbH, Germany, 2021-05)

TY  - JOUR
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Krnjaja, Vesna
AU  - Živković, Sanja
AU  - Trkulja, Vojislav
PY  - 2021-05
UR  - http://r.istocar.bg.ac.rs/handle/123456789/742
AB  - In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.
PB  - Verlag Eugen Ulmer GmbH, Germany
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia
DO  - 10.1007/s41348-021-00476-5
ER  - 
@article{
author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Živković, Sanja and Trkulja, Vojislav",
year = "2021-05",
abstract = "In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.",
publisher = "Verlag Eugen Ulmer GmbH, Germany",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia",
doi = "10.1007/s41348-021-00476-5"
}
Vasić, T., Jevremović, D., Krnjaja, V., Živković, S.,& Trkulja, V.. (2021-05). Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection
Verlag Eugen Ulmer GmbH, Germany..
https://doi.org/10.1007/s41348-021-00476-5
Vasić T, Jevremović D, Krnjaja V, Živković S, Trkulja V. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection. 2021;.
doi:10.1007/s41348-021-00476-5 .
Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Živković, Sanja, Trkulja, Vojislav, "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia" in Journal of Plant Diseases and Protection (2021-05),
https://doi.org/10.1007/s41348-021-00476-5 . .

Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia

Živković, Sanja; Vasić, Tanja; Trkulja, Vojislav; Krnjaja, Vesna; Marković, Jordan

(Ars Docendi, Bucharest, 2012)

TY  - JOUR
AU  - Živković, Sanja
AU  - Vasić, Tanja
AU  - Trkulja, Vojislav
AU  - Krnjaja, Vesna
AU  - Marković, Jordan
PY  - 2012
UR  - http://r.istocar.bg.ac.rs/handle/123456789/314
AB  - Pathogenicity of four isolates of Eutypa lata originating from Serbia (VL 17, VL 27, VL 29, VL 30) and two French reference isolates (8F and BX 1.10), were examined and compared on unrooted cuttings of Cabernet Sauvignon. Additionally, susceptibility to infection by all six isolates of E. lata was tested on unrooted cuttings of six different grapevine cultivars including Riesling white, Opuzenska rana, Sauvignon, Radmilovacki muskat, Beogradska besemena and Negotinski rubin in greenhouse experiments. All tested isolates proved to be pathogenic on Cabernet Sauvignon. Moreover, each grapevine cultivar showed different degrees of susceptibility to infection depending on the E. lata isolate. The effect of different culture media (PDA, MA, GWA, YA, WA and TA) on both sporulation of E. lata isolates and germination of conidia was also studied. Sporulation occurred after 30 days in three media (PDA, MA, GWA) and after 2 months on WA. No sporulation was observed on YA and TA. The ability of conidia to germinate was assessed on PDA, MA, GWA and WA under continuous UV light. Calculation revealed that the average percentage germination of conidia was very low 0,15%.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia
VL  - 17
IS  - 3
SP  - 7379
EP  - 7388
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_314
ER  - 
@article{
author = "Živković, Sanja and Vasić, Tanja and Trkulja, Vojislav and Krnjaja, Vesna and Marković, Jordan",
year = "2012",
abstract = "Pathogenicity of four isolates of Eutypa lata originating from Serbia (VL 17, VL 27, VL 29, VL 30) and two French reference isolates (8F and BX 1.10), were examined and compared on unrooted cuttings of Cabernet Sauvignon. Additionally, susceptibility to infection by all six isolates of E. lata was tested on unrooted cuttings of six different grapevine cultivars including Riesling white, Opuzenska rana, Sauvignon, Radmilovacki muskat, Beogradska besemena and Negotinski rubin in greenhouse experiments. All tested isolates proved to be pathogenic on Cabernet Sauvignon. Moreover, each grapevine cultivar showed different degrees of susceptibility to infection depending on the E. lata isolate. The effect of different culture media (PDA, MA, GWA, YA, WA and TA) on both sporulation of E. lata isolates and germination of conidia was also studied. Sporulation occurred after 30 days in three media (PDA, MA, GWA) and after 2 months on WA. No sporulation was observed on YA and TA. The ability of conidia to germinate was assessed on PDA, MA, GWA and WA under continuous UV light. Calculation revealed that the average percentage germination of conidia was very low 0,15%.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia",
volume = "17",
number = "3",
pages = "7379-7388",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_314"
}
Živković, S., Vasić, T., Trkulja, V., Krnjaja, V.,& Marković, J.. (2012). Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 17(3), 7379-7388.
https://hdl.handle.net/21.15107/rcub_ristocar_314
Živković S, Vasić T, Trkulja V, Krnjaja V, Marković J. Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia. in Romanian Biotechnological Letters. 2012;17(3):7379-7388.
https://hdl.handle.net/21.15107/rcub_ristocar_314 .
Živković, Sanja, Vasić, Tanja, Trkulja, Vojislav, Krnjaja, Vesna, Marković, Jordan, "Pathogenicity on grapevine and sporulation of E. lata isolates originating from Serbia" in Romanian Biotechnological Letters, 17, no. 3 (2012):7379-7388,
https://hdl.handle.net/21.15107/rcub_ristocar_314 .
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