TY  - CONF
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Krnjaja, Vesna
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/794
AB  - In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.
C3  - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina
T1  - Novel toxigenic species on maize kernels in Southeastern Europe
SP  - 194
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_794
ER  - 

@conference{
author = "Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Nikolić, Ana and Krnjaja, Vesna",
year = "2022",
abstract = "In recent years, global climate changes have caused the variability of agro-climatic conditions, 
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains 
during the growing season and could result in economic losses in the production, as well as in 
increased risk to human and animal health. These reasons and the fact that new toxigenic species 
have been identified in Serbia and its neighbouring countries in a few past years, indicate the 
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of 
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly 
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were 
caused by extremely stressful agrometeorological conditions, high temperatures and drought 
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from different samples of cereal kernels was done by using PCR-RFLP analysis 
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme 
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the 
presence of this species in the samples subjected to analysis. Characterization of Fg comlex 
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome 
fragments were sequenced and analized. Sequences were compared to the data from GeneBank. 
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while 
only two of them were identified as Fusarium boothii and Fusarium vorosii.",
journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina",
title = "Novel toxigenic species on maize kernels in Southeastern Europe",
pages = "194-194",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_794"
}

Stanković, S., Obradović, A., Nikolić, M., Nikolić, A.,& Krnjaja, V.. (2022). Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794

Stanković S, Obradović A, Nikolić M, Nikolić A, Krnjaja V. Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:194-194.
https://hdl.handle.net/21.15107/rcub_ristocar_794 .

Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Nikolić, Ana, Krnjaja, Vesna, "Novel toxigenic species on maize kernels in Southeastern Europe" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):194-194,
https://hdl.handle.net/21.15107/rcub_ristocar_794 .