TY  - JOUR
AU  - Stefanov, Rossen
AU  - Chervenkov, Mihail
AU  - Anev, Georgi
AU  - Maksimović, Nevena
AU  - Andreeva, Madlena
AU  - Ivanova, Teodora
AU  - Milovanović, Aleksandar
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/565
AB  - Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemented as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (Na2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and pH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39˚S for 360 min. It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment.
AB  - Selen je element u tragovima, koji stimuliše antioksidantske odbrane i poboljšava reproduktivne funkcije kod ljudi i životinja, u obliku selenoproteina. Cilj studije je bio da se proceni efekat selena, dopunjenog u neorganskom ili organskom oblik u ishrani ovnova, na neke parametre semena. Eksperiment je obavljen sa 15 klinički zdravih ovnova severnoistočne bugarske merino rase. Životinje su podeljene u tri grupe (5 po grupi). Ovnovi prve eksperimentalne grupe (G1) dobijali su obrok sa dodatkom 4,0 mg natrijum selenita (Na2SeO3) po grlu dnevno, dok su životinje druge eksperimentalne grupe (G2) hranjene obrokom sa 1,83 g L-selenometionina (Sel- Plek, Alltech, SAD) po grlu dnevno. Na kraju, svaka životinja iz G1 i G2 dobijala je 1,83g selena dnevno. Kontrolna grupa (GC) dobila je obrok bez dodatka selena. Glavni sastav obroka u svakoj grupi bio je isti. Ejakulati su dobijeni preko veštačke vagine. Ocenjivani su sledeći parametri: volumen i pH ejakulata i pokretljivosti, koncentracija i preživljavanje in vitro spermatozoida na 39°C tokom 360 min. Utvrđeno je da dodatak obroku neorganskog i organskog selena doveo do povećanja zapremine ejakulata, pokretljivosti i preživljavanja spermatozoida. Na sveže dobijeno seme nije uticao tretman sa selenom.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams
T1  - Uticaj dodavanja neorganskog i organskog selena na kvalitet i količinu sperme ovnova severoistočne bugarske merino rase
VL  - 34
IS  - 1
SP  - 69
EP  - 81
DO  - 10.2298/bah1801069S
ER  - 

@article{
author = "Stefanov, Rossen and Chervenkov, Mihail and Anev, Georgi and Maksimović, Nevena and Andreeva, Madlena and Ivanova, Teodora and Milovanović, Aleksandar",
year = "2018",
abstract = "Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemented as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (Na2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and pH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39˚S for 360 min. It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment., Selen je element u tragovima, koji stimuliše antioksidantske odbrane i poboljšava reproduktivne funkcije kod ljudi i životinja, u obliku selenoproteina. Cilj studije je bio da se proceni efekat selena, dopunjenog u neorganskom ili organskom oblik u ishrani ovnova, na neke parametre semena. Eksperiment je obavljen sa 15 klinički zdravih ovnova severnoistočne bugarske merino rase. Životinje su podeljene u tri grupe (5 po grupi). Ovnovi prve eksperimentalne grupe (G1) dobijali su obrok sa dodatkom 4,0 mg natrijum selenita (Na2SeO3) po grlu dnevno, dok su životinje druge eksperimentalne grupe (G2) hranjene obrokom sa 1,83 g L-selenometionina (Sel- Plek, Alltech, SAD) po grlu dnevno. Na kraju, svaka životinja iz G1 i G2 dobijala je 1,83g selena dnevno. Kontrolna grupa (GC) dobila je obrok bez dodatka selena. Glavni sastav obroka u svakoj grupi bio je isti. Ejakulati su dobijeni preko veštačke vagine. Ocenjivani su sledeći parametri: volumen i pH ejakulata i pokretljivosti, koncentracija i preživljavanje in vitro spermatozoida na 39°C tokom 360 min. Utvrđeno je da dodatak obroku neorganskog i organskog selena doveo do povećanja zapremine ejakulata, pokretljivosti i preživljavanja spermatozoida. Na sveže dobijeno seme nije uticao tretman sa selenom.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams, Uticaj dodavanja neorganskog i organskog selena na kvalitet i količinu sperme ovnova severoistočne bugarske merino rase",
volume = "34",
number = "1",
pages = "69-81",
doi = "10.2298/bah1801069S"
}

Stefanov, R., Chervenkov, M., Anev, G., Maksimović, N., Andreeva, M., Ivanova, T.,& Milovanović, A.. (2018). Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 34(1), 69-81.
https://doi.org/10.2298/bah1801069S

Stefanov R, Chervenkov M, Anev G, Maksimović N, Andreeva M, Ivanova T, Milovanović A. Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams. in Biotechnology in Animal Husbandry. 2018;34(1):69-81.
doi:10.2298/bah1801069S .

Stefanov, Rossen, Chervenkov, Mihail, Anev, Georgi, Maksimović, Nevena, Andreeva, Madlena, Ivanova, Teodora, Milovanović, Aleksandar, "Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in North-East Bulgarian merino rams" in Biotechnology in Animal Husbandry, 34, no. 1 (2018):69-81,
https://doi.org/10.2298/bah1801069S . .

TY  - JOUR
AU  - Manchev, Stefan
AU  - Stefanov, Rosen
AU  - Anev, Georgi
AU  - Tzvetkova, Petya
AU  - Maksimović, Nevena
PY  - 2014
UR  - http://r.istocar.bg.ac.rs/handle/123456789/432
AB  - Some ruminant species, including sheep, show a markedly seasonal breeding cycle. There is evidence for the practical use of the pineal hormone melatonin to improve reproduction in sheep but the mechanism of action of the hormone in rams is less known. Studies have shown significant breed differences as a result of its application. The purpose of this study is to obtain more comprehensive data about the effects of melatonin on the fertility of rams of Northeastern Bulgarian fine fleece breed characterized by a marked seasonal cycle of reproduction. Detailed andrological studies involving the breed are absent and no statements and results of studies published on this subject could be found in the literature so far. We tested the capabilities of melatoninadministered both by diet and injection of a subcutaneous implant containing the hormone (Melovin, Ceva Animal Health, France). Dilution of semen was made using thinner buffer base containing a combination of carbohydrates. Results show increased motility, increased survival and a decrease in the percentage variation in the ejaculate pathological forms in the treated animals. Slightly better results are obtained from the implanted animals compared to rams receiving hormone alimentary. Protecting and promoting properties of the hormone are important for the reproductive capacity and improve the reproductive performance of rams. Results and statements presented in this study can be used mainly by livestock rearing this breed, but the experience learned from the research will also be useful for farmers working on breeding and other local Bulgarian sheep breeds.
PB  - Publ House Bulgarian Acad Sci, Sofia
T2  - Comptes Rendus de l Academie Bulgare des Sciences
T1  - Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed
VL  - 67
IS  - 12
SP  - 1701
EP  - 1706
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_432
ER  - 

@article{
author = "Manchev, Stefan and Stefanov, Rosen and Anev, Georgi and Tzvetkova, Petya and Maksimović, Nevena",
year = "2014",
abstract = "Some ruminant species, including sheep, show a markedly seasonal breeding cycle. There is evidence for the practical use of the pineal hormone melatonin to improve reproduction in sheep but the mechanism of action of the hormone in rams is less known. Studies have shown significant breed differences as a result of its application. The purpose of this study is to obtain more comprehensive data about the effects of melatonin on the fertility of rams of Northeastern Bulgarian fine fleece breed characterized by a marked seasonal cycle of reproduction. Detailed andrological studies involving the breed are absent and no statements and results of studies published on this subject could be found in the literature so far. We tested the capabilities of melatoninadministered both by diet and injection of a subcutaneous implant containing the hormone (Melovin, Ceva Animal Health, France). Dilution of semen was made using thinner buffer base containing a combination of carbohydrates. Results show increased motility, increased survival and a decrease in the percentage variation in the ejaculate pathological forms in the treated animals. Slightly better results are obtained from the implanted animals compared to rams receiving hormone alimentary. Protecting and promoting properties of the hormone are important for the reproductive capacity and improve the reproductive performance of rams. Results and statements presented in this study can be used mainly by livestock rearing this breed, but the experience learned from the research will also be useful for farmers working on breeding and other local Bulgarian sheep breeds.",
publisher = "Publ House Bulgarian Acad Sci, Sofia",
journal = "Comptes Rendus de l Academie Bulgare des Sciences",
title = "Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed",
volume = "67",
number = "12",
pages = "1701-1706",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_432"
}

Manchev, S., Stefanov, R., Anev, G., Tzvetkova, P.,& Maksimović, N.. (2014). Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed. in Comptes Rendus de l Academie Bulgare des Sciences
Publ House Bulgarian Acad Sci, Sofia., 67(12), 1701-1706.
https://hdl.handle.net/21.15107/rcub_ristocar_432

Manchev S, Stefanov R, Anev G, Tzvetkova P, Maksimović N. Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed. in Comptes Rendus de l Academie Bulgare des Sciences. 2014;67(12):1701-1706.
https://hdl.handle.net/21.15107/rcub_ristocar_432 .

Manchev, Stefan, Stefanov, Rosen, Anev, Georgi, Tzvetkova, Petya, Maksimović, Nevena, "Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed" in Comptes Rendus de l Academie Bulgare des Sciences, 67, no. 12 (2014):1701-1706,
https://hdl.handle.net/21.15107/rcub_ristocar_432 .