BACILLUS CEREUS AND SELENIUM HAVE GOOD EFFECT ON IN VITRO MATURATION OF OOCYTES

Abstract

It is well known that selenium has positive effect on the reproductive tract either in female or male individuals, but cytology and biochemistry are not well elucidated. In this paper cytologically and microbiologically is shown that bacteria Bacillus cereus and selenium have large positive and synergistic influence on maturation of oocytes in vitro. This is caused by the bactericide effect on coliform bacteria which are abundant in the digestive tract. Taking account that anus and vagina are often nearby each other and that even sterility can be caused by coliform bacteria in the upper parts of the female reproductive tract, we found microbiologically and cytologically in oocyte in the presence of coliform bacteria, and Bacillus cereus and selenium, that number of coliform bacteria decreases, and cytologically oocytes cultured in such medium in vitro have more vitality than in culture medium without at least selenium supplement. These investigations should be continued with the aim of optimization of conditions for maturation of oocytes and fertilization in vitro for embryo transfer of domestic animals as well as model for in vivo reproduction.