Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
2016
Authors
Vasić, TanjaKrnjaja, Vesna
Jevremović, Darko
Stanković, Slavica Ž.
Terzic, Dragan
Milenković, Jasmina
Marković, Jordan
Article (Published version)
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A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geog...raphical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
Keywords:
RFLP / VCGs / M. sativa / T. pratense / C. destructivumSource:
Genetika-Belgrade, 2016, 48, 1, 187-198Publisher:
- Serbian Genetics Society, Belgrade
Funding / projects:
- Improvement of genetic potential and technologies in forage crops production in function of sustainable animal husbandry development (RS-MESTD-Technological Development (TD or TR)-31057)
DOI: 10.2298/GENSR1601187V
ISSN: 0534-0012
WoS: 000376744800016
Scopus: 2-s2.0-84971219286
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RIStocarTY - JOUR AU - Vasić, Tanja AU - Krnjaja, Vesna AU - Jevremović, Darko AU - Stanković, Slavica Ž. AU - Terzic, Dragan AU - Milenković, Jasmina AU - Marković, Jordan PY - 2016 UR - http://r.istocar.bg.ac.rs/handle/123456789/484 AB - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates. PB - Serbian Genetics Society, Belgrade T2 - Genetika-Belgrade T1 - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover VL - 48 IS - 1 SP - 187 EP - 198 DO - 10.2298/GENSR1601187V ER -
@article{ author = "Vasić, Tanja and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica Ž. and Terzic, Dragan and Milenković, Jasmina and Marković, Jordan", year = "2016", abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.", publisher = "Serbian Genetics Society, Belgrade", journal = "Genetika-Belgrade", title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover", volume = "48", number = "1", pages = "187-198", doi = "10.2298/GENSR1601187V" }
Vasić, T., Krnjaja, V., Jevremović, D., Stanković, S. Ž., Terzic, D., Milenković, J.,& Marković, J.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade Serbian Genetics Society, Belgrade., 48(1), 187-198. https://doi.org/10.2298/GENSR1601187V
Vasić T, Krnjaja V, Jevremović D, Stanković SŽ, Terzic D, Milenković J, Marković J. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade. 2016;48(1):187-198. doi:10.2298/GENSR1601187V .
Vasić, Tanja, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica Ž., Terzic, Dragan, Milenković, Jasmina, Marković, Jordan, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika-Belgrade, 48, no. 1 (2016):187-198, https://doi.org/10.2298/GENSR1601187V . .