Novel toxigenic species on maize kernels in Southeastern Europe
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In recent years, global climate changes have caused the variability of agro-climatic conditions,
which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains
during the growing season and could result in economic losses in the production, as well as in
increased risk to human and animal health. These reasons and the fact that new toxigenic species
have been identified in Serbia and its neighbouring countries in a few past years, indicate the
need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of
the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly
high frequency and incidence of Aspergillus infestation of maize grain in the last few years were
caused by extremely stressful agrometeorological conditions, high temperatures and drought
over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus
species collected from di...fferent samples of cereal kernels was done by using PCR-RFLP analysis
of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme
gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the
presence of this species in the samples subjected to analysis. Characterization of Fg comlex
species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome
fragments were sequenced and analized. Sequences were compared to the data from GeneBank.
Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while
only two of them were identified as Fusarium boothii and Fusarium vorosii.
Ključne reči:
maize / Aspergillus / FusariumIzvor:
Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 2022, 194-194Institucija/grupa
RIStocarTY - CONF AU - Stanković, Slavica AU - Obradović, Ana AU - Nikolić, Milica AU - Nikolić, Ana AU - Krnjaja, Vesna PY - 2022 UR - http://r.istocar.bg.ac.rs/handle/123456789/794 AB - In recent years, global climate changes have caused the variability of agro-climatic conditions, which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains during the growing season and could result in economic losses in the production, as well as in increased risk to human and animal health. These reasons and the fact that new toxigenic species have been identified in Serbia and its neighbouring countries in a few past years, indicate the need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly high frequency and incidence of Aspergillus infestation of maize grain in the last few years were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus species collected from different samples of cereal kernels was done by using PCR-RFLP analysis of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the presence of this species in the samples subjected to analysis. Characterization of Fg comlex species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome fragments were sequenced and analized. Sequences were compared to the data from GeneBank. Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while only two of them were identified as Fusarium boothii and Fusarium vorosii. C3 - Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina T1 - Novel toxigenic species on maize kernels in Southeastern Europe SP - 194 EP - 194 UR - https://hdl.handle.net/21.15107/rcub_ristocar_794 ER -
@conference{ author = "Stanković, Slavica and Obradović, Ana and Nikolić, Milica and Nikolić, Ana and Krnjaja, Vesna", year = "2022", abstract = "In recent years, global climate changes have caused the variability of agro-climatic conditions, which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains during the growing season and could result in economic losses in the production, as well as in increased risk to human and animal health. These reasons and the fact that new toxigenic species have been identified in Serbia and its neighbouring countries in a few past years, indicate the need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly high frequency and incidence of Aspergillus infestation of maize grain in the last few years were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus species collected from different samples of cereal kernels was done by using PCR-RFLP analysis of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the presence of this species in the samples subjected to analysis. Characterization of Fg comlex species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome fragments were sequenced and analized. Sequences were compared to the data from GeneBank. Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while only two of them were identified as Fusarium boothii and Fusarium vorosii.", journal = "Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina", title = "Novel toxigenic species on maize kernels in Southeastern Europe", pages = "194-194", url = "https://hdl.handle.net/21.15107/rcub_ristocar_794" }
Stanković, S., Obradović, A., Nikolić, M., Nikolić, A.,& Krnjaja, V.. (2022). Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina, 194-194. https://hdl.handle.net/21.15107/rcub_ristocar_794
Stanković S, Obradović A, Nikolić M, Nikolić A, Krnjaja V. Novel toxigenic species on maize kernels in Southeastern Europe. in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina. 2022;:194-194. https://hdl.handle.net/21.15107/rcub_ristocar_794 .
Stanković, Slavica, Obradović, Ana, Nikolić, Milica, Nikolić, Ana, Krnjaja, Vesna, "Novel toxigenic species on maize kernels in Southeastern Europe" in Book of Abstracts of the XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Jahorina, October 06 - 09, 2022, Bosnia and Herzegovina (2022):194-194, https://hdl.handle.net/21.15107/rcub_ristocar_794 .