The Tuva Republic is one of the main sheep breeding centers in the Russian
Federation. The Tuvan short-fat-tailed sheep is a unique local breed of the Tuva Republic.
The studied breed has sheep of steppe type and mountain type. Each type of sheep was
having formed according to relief of the ground and environment features. The
breed originated in different parts from steppe and mountains. This study aim was to
show the genetic polymorphism of calpastatin gene (CAST) in ram populations of two
main breeding centers for tuvan short-fat-tailed sheep. Genomic DNA was isolated from
51 animals from Municipal unitary enterprise "Despen" (steppe type of sheep), and 100
animals from State unitary enterprise "Malchyn" (mountain type of sheep). Genomic
DNA was extracted using the commercial kits. The detection was done using the
restriction fragment length polymorphism of the polymerase chain reaction products
(PCR-RFLPs). We used a pair of primers: F: 5’-TGGGGCC
CAATGACGCCATCGATG-3’ a...nd R: 5’-GGTGGAGCAGCACTTCTGATCACC-3’ to
get 622 b.p. fragment of CAST gene. The PCR products are having digested with MspI restriction enzyme. Calpastatin locus digested with MspI has shown
three genotypes: MM, MN and NN. The frequency of MM, MN and NN genotypes were
80.39%, 17.65% and 1.96% in steppe type of breed and 77.00%, 22.00% and 1.00% in
the mountain type of breed. The highest allelic frequency was for allele M (0.89
in MUE "Despen" rams and 0.88 in SUE "Malchyn" rams). Allelic frequency of allele N
had lower values of 0.11 in steppe type and 0.12 in mountain type.
TY - JOUR
AU - Kulikova, Ksenia A.
AU - Yuldashbaev, Yusupjan A.
AU - Petrović, Milan P.
AU - Caro-Petrović, Violeta
AU - Ružić-Muslić, Dragana
AU - Maksimović, Nevena
AU - Ostojić Andrić, Dušica
PY - 2018
UR - http://r.istocar.bg.ac.rs/handle/123456789/725
AB - The Tuva Republic is one of the main sheep breeding centers in the Russian
Federation. The Tuvan short-fat-tailed sheep is a unique local breed of the Tuva Republic.
The studied breed has sheep of steppe type and mountain type. Each type of sheep was
having formed according to relief of the ground and environment features. The
breed originated in different parts from steppe and mountains. This study aim was to
show the genetic polymorphism of calpastatin gene (CAST) in ram populations of two
main breeding centers for tuvan short-fat-tailed sheep. Genomic DNA was isolated from
51 animals from Municipal unitary enterprise "Despen" (steppe type of sheep), and 100
animals from State unitary enterprise "Malchyn" (mountain type of sheep). Genomic
DNA was extracted using the commercial kits. The detection was done using the
restriction fragment length polymorphism of the polymerase chain reaction products
(PCR-RFLPs). We used a pair of primers: F: 5’-TGGGGCC
CAATGACGCCATCGATG-3’ and R: 5’-GGTGGAGCAGCACTTCTGATCACC-3’ to
get 622 b.p. fragment of CAST gene. The PCR products are having digested with MspI restriction enzyme. Calpastatin locus digested with MspI has shown
three genotypes: MM, MN and NN. The frequency of MM, MN and NN genotypes were
80.39%, 17.65% and 1.96% in steppe type of breed and 77.00%, 22.00% and 1.00% in
the mountain type of breed. The highest allelic frequency was for allele M (0.89
in MUE "Despen" rams and 0.88 in SUE "Malchyn" rams). Allelic frequency of allele N
had lower values of 0.11 in steppe type and 0.12 in mountain type.
PB - Savez društva genetičara, Beograd, Republika Srbija
T2 - Genetika
T1 - The polymorphism of cast gene in rams populations of tuvan breed
VL - 50
DO - https://doi.org/10.2298/GENSR1803885K
ER -
@article{
author = "Kulikova, Ksenia A. and Yuldashbaev, Yusupjan A. and Petrović, Milan P. and Caro-Petrović, Violeta and Ružić-Muslić, Dragana and Maksimović, Nevena and Ostojić Andrić, Dušica",
year = "2018",
abstract = "The Tuva Republic is one of the main sheep breeding centers in the Russian
Federation. The Tuvan short-fat-tailed sheep is a unique local breed of the Tuva Republic.
The studied breed has sheep of steppe type and mountain type. Each type of sheep was
having formed according to relief of the ground and environment features. The
breed originated in different parts from steppe and mountains. This study aim was to
show the genetic polymorphism of calpastatin gene (CAST) in ram populations of two
main breeding centers for tuvan short-fat-tailed sheep. Genomic DNA was isolated from
51 animals from Municipal unitary enterprise "Despen" (steppe type of sheep), and 100
animals from State unitary enterprise "Malchyn" (mountain type of sheep). Genomic
DNA was extracted using the commercial kits. The detection was done using the
restriction fragment length polymorphism of the polymerase chain reaction products
(PCR-RFLPs). We used a pair of primers: F: 5’-TGGGGCC
CAATGACGCCATCGATG-3’ and R: 5’-GGTGGAGCAGCACTTCTGATCACC-3’ to
get 622 b.p. fragment of CAST gene. The PCR products are having digested with MspI restriction enzyme. Calpastatin locus digested with MspI has shown
three genotypes: MM, MN and NN. The frequency of MM, MN and NN genotypes were
80.39%, 17.65% and 1.96% in steppe type of breed and 77.00%, 22.00% and 1.00% in
the mountain type of breed. The highest allelic frequency was for allele M (0.89
in MUE "Despen" rams and 0.88 in SUE "Malchyn" rams). Allelic frequency of allele N
had lower values of 0.11 in steppe type and 0.12 in mountain type.",
publisher = "Savez društva genetičara, Beograd, Republika Srbija",
journal = "Genetika",
title = "The polymorphism of cast gene in rams populations of tuvan breed",
volume = "50",
doi = "https://doi.org/10.2298/GENSR1803885K"
}
Kulikova, K. A., Yuldashbaev, Y. A., Petrović, M. P., Caro-Petrović, V., Ružić-Muslić, D., Maksimović, N.,& Ostojić Andrić, D.. (2018). The polymorphism of cast gene in rams populations of tuvan breed. in Genetika
Savez društva genetičara, Beograd, Republika Srbija., 50.
https://doi.org/https://doi.org/10.2298/GENSR1803885K
Kulikova KA, Yuldashbaev YA, Petrović MP, Caro-Petrović V, Ružić-Muslić D, Maksimović N, Ostojić Andrić D. The polymorphism of cast gene in rams populations of tuvan breed. in Genetika. 2018;50.
doi:https://doi.org/10.2298/GENSR1803885K .
Kulikova, Ksenia A., Yuldashbaev, Yusupjan A., Petrović, Milan P., Caro-Petrović, Violeta, Ružić-Muslić, Dragana, Maksimović, Nevena, Ostojić Andrić, Dušica, "The polymorphism of cast gene in rams populations of tuvan breed" in Genetika, 50 (2018),
https://doi.org/https://doi.org/10.2298/GENSR1803885K . .
