Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” Fish Farm, Serbia
Authors
Molerović, NikolaRaskovic, Bozidar
Đedović, Radica
Ostojić Andrić, Dušica

Marković, Zoran
Marić, Saša
Article (Published version)
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Due to the ecological concerns and preservation of genetic
resources, the characterization of genetic structure of the brown trout (Salmo trutta)
was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of
10 individuals, and after that molecular PCR-RFLP methods were used for
distinguishing between Atlantic and Danubian lineages based on control region of
the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the
nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated
that out of 10 individuals included in this study five belonged to the allochtonous
Atlantic lineage and remaining five belonged to the native Danubian lineage of
brown trout. However, results of molecular analyses showed a high percentage of
allochthonous genes among the individuals, which confirms the hybridization
between these two lineages. Also, the results showed that the selection based on the
phenotype is not adequate. In order to continue with pr...oper broodstock
management, it is necessary to eliminate allochtonous individuals of the Atlantic
lineage from the broodstock.
Keywords:
Salmo trutta / KR mtDNK / LDH nDNK / PCR / RFLP / selekcijaSource:
Biotechnology in Animal Husbandry, 2019, 35, 3, 289-299Publisher:
- Institute for Animal Husbandry, Belgrade-Zemun