Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia
Ostojić Andrić, Dušica
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The objective of this study was to determine the frequency of the kappa-casein and beta-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA kappa-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and beta-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for beta-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00...% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population.
Keywords:beta-lactoglobulin / kappa-casein / polymorphism / PCR-RFLP / Simmental breed
Source:Genetika-Belgrade, 2018, 50, 2, 659-668
- Serbian Genetics Society, Belgrade